首页|Atomic and molecular mechanisms underlying the osteogenic effects of bioglasscircleR materials
Atomic and molecular mechanisms underlying the osteogenic effects of bioglasscircleR materials
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The aim of this in vivo study was to determine the atomic and molecular mechanisms underlying the osteogenic effects of BioglasscircleR materials。 Sol-gel BioglasscircleR， PerioGlascircleR， and hydroxyapatite (HA) were implanted endosteally in bilateral proximal tibial condyles of twenty-four rabbits for two and eight weeks。 Surgical trauma at the same location without implant materials was included as a control。 New bone formation was evaluated histomorphometrically。 Expressions of two bone formation markers， osteocalcin (OC) and alkaline phosphatase (AKP)， were measured immunohistochemically。 Distributions of material elements at the interfaces were identified with EDAX on nondecalcified tissue sections。 The results showed that at 2 weeks， higher silica-containing sol-gel BioglasscircleR generated the greatest amount of woven bone and cartilage which was replaced by dense compact bone at 8 weeks。 PerioGlascircleR yielded a higher level of intramembranous bone than HA and surgery alone。 OC expression was significantly up-regulated by sol-gel BioglasscircleR (p<0。01) and PerioGlascircleR (p<0。05)。 AKP expression was essentially at the same level among the groups tested。 EDAX analysis indicated that solgel BioglasscircleR quickly released a high level of Si which then distributed to the areas of surrounding tissue， followed by an increased concentration of Ca and P indicating an early bone formation。 High S reading at sol-gel BioglasscircleR interfaces also indicated a higher activity of endochondral bone formation which was consistent with histological findings at 2 weeks。 The results suggested that sol-gel BioglasscircleR may have an early and significant osteogenic effect by releasing a higher level of Si and stimulating OC synthesis， resulted in a greater amount of new bone formation。
NETL
