首页|Osteogenic potential of cultured marrow stromal stem cells on the surface of zinc-containing bioceramics

Osteogenic potential of cultured marrow stromal stem cells on the surface of zinc-containing bioceramics

扫码查看
原文链接
  • NETL
We prepared zinc-containing TCP/HAp ceramics (Zn-TCP/HAp) in the shape of disk， and examined the effect of zinc on the osteogenic differentiation of cultured marrow cells on the surface of Zn-TCP/HAp。 Fresh marrow cells were obtained from the femora of Fischer rats and cultured in a medium containing 15percent fetal bovine serum to reach confluent。 After trypsinization， the cells were seeded at 20 x 10~3 cells/16 mm PHI on Falcon tissue wells with the ceramic disk for subculture。 Just before the cell seeding， three different disks (TCP/HAp containing 0， 0。126， or 0。316 wtpercent Zn) were placed in the wells。 After 2 weeks of subculture in the presence of beta -glycerophosphate， vitamin C phosphate， and dexamethasone， the cells were stained for alkaline phosphatase (ALP)。 The ALP positive area on the Zn-TCP/HAp disk was expanded with an increase in Zn content， as covering almost the entire surface of the Zn-TCP/HAp disk of 0。316 Zn wtpercent。 The results indicate that the Zn doped TCP/HAp ceramics stimulate osteoblastic differentiation in cultured marrow stromal cells。

zincTCP/HApalkaline phosphataseosteogenesis

M. Ikeuchi、A. Ito、H. Ohgushi、Y. Dohi、T. Noshi、K. Horiuchi、M. Sugimura、K. Yonemasu

展开 >

International symposium on ceramics in medicine;Workshop for ceramics in joint prostheses

Nara City(JP);Singapore(SG)

12th International Symposium on Ceramics in Medicine at Nara City, Japan 8-11 October 1999 and Workshop for Ceramics in Joint Prostheses at Singapore 13-14 October, 1999

p.207-210

1999