SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION IN PINUS ARMANDII VAR. AMAMIANA

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外文摘要：Embryogenic cell suspensions were induced from a mature seed of Pinus armandii var。 amamiana known as an endangered species。 MS liquid medium supplemented with 10 μ M 2，4-D and 3% sucrose was used for initiation of the suspensions。 The suspensions were incubated in the dark at 24℃。 Obtained suspensions were transferred to NH_4NO_3-free MS liquid medium supplemented with 10 μ M 2，4-D， 3mM L-glutamine and 3% sucrose and subcultured every 2 weeks。 In order to develop somatic embryos， the suspensions were transferred into NH_4NO_3-free MS media supplemented with 3% sucrose， lOO μ M ABA， 0-20% PEG (MW4，000)， 0-20mM L-glutamine and 0。2% activated charcoal。 After 1-2 weeks of incubation the suspension cultures were transferred onto NH_4NO_3-free media solidified with 0。4% gellan gum containing 100 μ M ABA， 0。2% activated charcoal， 0-10% PEG and 0-20mM L-glutamine。 The cultures were incubated under a 16h light / 8h dark photoperiod。 After 1-2 momths of culture， differentiation of embryos progressed and cotyledonary embryos were obtained。 These embryos were transferred on NH_4NO_3-free solid MS media containing 0-10mM L-glutamine and incubated at 24℃ and under a 16h light / dark photoperiod。 After 2-3 weeks some plantlets with roots and green cotyledons were obtained。 Plantlets were transplanted into vermiculite containing modified MS liquid medium in 200ml culture flasks。 This is the first report of somatic embryo genesis and plantlet regeneration in P。 armandii var。 amamiana。

外文关键词：

pinus armandii var. amamianaendangered speciessomatic embryogenesisMS-murashige and skoog medium

作者：

Yoshihisa Hosoi、Katsuaki Ishii

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作者单位：

Division of Bio-resources Technology, Forestry and Forest Products Research Institute, P.O.Box 16, Tsukuba Norin Kenkyu Danchi-nai, Ibaraki, 305-8687 JAPAN

会议名称：

Molecular Breeding of Woody Plants

会议地点：

Narita(JP);Narita(JP)

会议母体文献：

International Wood Biotechnology Symposium (IWBS) Mar 14-17, 2001, Narita, Chiba, Japan

页码：

p.313-318

出版时间：

2001