Pulsed Electrochemical Detection in bioanalysis: chemical fingerprinting

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外文摘要：Pulsed Electrochemical Detection (PED) is a revolutionary approach to the simple， sensitive， and direct detection of numerous polar aliphatic compounds， especially carbohydrates。 This technique exploits the electrocatalytic activity of noble metal electrode surfaces to oxidize various polar functional groups。 In PED， multi-step potential-time waveforms at Au and Pt electrodes realize amperometric/coulometric detection while maintaining uniform and reproducible electrode activity。 The response mechanisms in PED are dominated by the surface properties of the electrode， and， as a consequence， members of each chemical class of compounds produce virtually identical voltammetric responses。 Thus， the full potential is realized when combined with high performance liquid chromatography (HPLC)。 This paper reviews the fundamental aspects of PED and details a novel approach to the chemical "fingerprinting" of natural products。 Applications include the characterization of tobacco， peptones， and bacteria。

外文关键词：

pulsed electrochemical detectioncarbohydratescarbo-rypingHPLCpeptonestobaccobacteria

作者：

William R. LaCourse

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作者单位：

Dept. of Chemistry, University of Maryland Baltimore County, Baltimore, MD 21250

会议名称：

Smart Medical and Biomedical Sensor Technology

会议地点：

Providence, RI(US)

会议母体文献：

Conference on Smart Medical and Biomedical Sensor Technology; Oct 28-29, 2003; Providence, Rhode Island, USA

页码：

p.103-112

出版时间：

2003