The effect of 2,4-D and NAA concentration on callus induction and somatic embryogenesis differentiation of alfalfa

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外文摘要：The effect of 2，4-D and NAA concentration on callus induction of cotyledons and the differentiation of somatic embryogenesis was studied by tissue culture techniques。 The results suggested that 2，4-D was the main factor in the inducing period。 But NAA had the special function to induce somatic embryogenesis。 The somatic embryogenesis inducing rate increased with the increase in NAA concentration and induction rate was over 50% of Mi-lu。 Fu - ping， Gan-nong No。 3。 The effective callus inducing media was MS+2，4-D2。0 mg • L~(-1) + NAA1。0 mg • L~(-1) + sucrose 30 g • L~(-1)。

外文关键词：

2,4-DNAAcallussomatic embryogenesis

作者：

ZHANG Jingni、LU Xinshi、MA Huiling、CAO Zhizhong

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作者单位：

Natur 1 Resource and Environmental College, Beijing Forestry University, Beijing 100083, China

会议名称：

Acta Prataculturae Sinica vol.15 Suppl. July 2006: Grassland Agriculture: Balancing Production and Environmental Protection

会议地点：

Lanzhou(CN)

会议母体文献：

China-Japan-Korea Grassland Conference; 20060731-0806; Lanzhou(CN)

页码：

P.258-259

出版时间：

2006