Detection of inorganic ions on a capillary electrophoresis microchip using a conductivity technique

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外文摘要：Microchip-based electrophoretic separation systems are essential components in the development of fully integrated micro total analysis systems。 In this paper， a miniaturized analytical system for separating and detecting inorganic ions is described。 The system was based on a polycarbonate (PC) capillary electrophoresis (CE) chip and a contactless conductivity detector， both being developed at CSIRO Microfluidics and Microfabrication Laboratories， Melbourne， Australia。 The PC chip was fabricated using the soft lithography technique in conjunction with nickel plating and hot embossing。 The detector electrodes were fabricated from a PCB board and attached on the separation chip bottom surface。 The thin capping layer (20 micron) of the chip allowed for sensitive detection of conductivity change。 The system was demonstrated to separate reliably the potassium， sodium and lithium ions in a 20mM MES/His buffer within a minute at an electrical field of 28。5kV/m。 The detection limit for the current design is around 100μM。 Such a system offers great promise to be integrated into robust hand-held devices for in-situ monitoring of chemical and biological samples with high speed， reliability and low costs。

外文关键词：

microfluidic chipseparationcapillary electrophoresiscontactless conductivity detection

作者：

Petkovic-Duran、K.、Y. Zhu、A. Swallow、N. Noui-Mehidi、P. Leech、F. Glenn

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作者单位：

CSIRO Microfluidics Laboratory, CSIRO Division of Manufacturing and Materials Technology, 37 Graham Road, PO Box 56, Highett, Melbourne, VIC 3190, Australia

会议名称：

Conference on Biomedical Applications of Micro- and Nanoengineering III

会议地点：

Adelaide(AU)

会议母体文献：

Biomedical Applications of Micro- and Nanoengineering III; Progress in Biomedical Optics and Imaging; vol.7 no.39; Proceedings of SPIE-The International Society for Optical Engineering; vol.6416

页码：

64160J.1-64160J.6

出版时间：

2006