Mitochondrial Ca~(2+) Flux through Na~+/Ca~(2+) Exchange

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原文链接

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外文摘要：To clarify the property of mitochondrial Na~+/Ca~(2+) exchange in situ， we measured mitochondrial Ca~(2+) using Rhod-2 in permeabi-lized rat ventricular myocytes。 Cytoplasmic 300 nM Ca~(2+) (Ca~(2+)_c) augmented the Rhod-2 intensity by ~ninefolds without cytoplasmic Na~+ (Na~+_c)。 Increasing Na~+_c attenuated the maximum level of Rhod-2 fluorescence， probably due to the activation of forward mode of mitochondrial Na~+/Ca~(2+) exchange。 The Rhod-2 intensity decayed upon removing Ca~(2+)_c。 The decay was dependent on Na~+_c (K_(1/2) = ～1 mM) and largely abolished by an inhibitor of mitochondrial Na~+/Ca~(2+) exchange， CGP-37157。 It was suggested that Na~+ binding to the mitochondrial Na~+/Ca~(2+) exchange is saturated in the physiological concentration of Na~+_c。

外文关键词：

mitochondrial Ca~(2+)mitochondrial Na~+/Ca~(2+) exchangerCa~(2+) uniporterruthenium redCGP-37157

作者：

BONGJU KIM、SATOSHI MATSUOKA

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作者单位：

Department of Physiology and Biophysics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan

会议名称：

International Conference on Na/Ca Exchange 2006

会议地点：

Brussels(BE)

会议母体文献：

Sodium-Calcium Exchange and the Plasma Membrane Ca~(2+)- ATPase in Cell Function; Annals of the New York Academy of Sciences; vol.1099

页码：

507-511

出版时间：

2006