首页|Urinary 8-Hydroxy-2'-deoxyguanosine as a biomarker for oxidative DNA damage in populations with long-term exposure to mercury

Urinary 8-Hydroxy-2'-deoxyguanosine as a biomarker for oxidative DNA damage in populations with long-term exposure to mercury

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Mercury is a ubiquitous and highly toxic environmental pollutant. To study the relationship between mercury exposure and oxidative stress, serum and urinary Hg contents, oxidative DNA damage, and serum redox status have been evaluated in chronically Hg-exposed subiects and compared with the healthy controls. Urinary 8-hydroxy-2'-deoxyguanosine (8- OHdG) content, which was used as a biomarker of oxidative DNA damage among the Hg-exposed people, was determined by HPLC coupled with electrochemical detection (ECD). The antioxidant status was evaluated by measuring the activities of superoxide dismutase and glutathione peroxidase and the concentrations of total reduced glutathione and protein-bound thiols in serum. Our data demon strate that Hg exposure can induce oxidative DNA damage, while the antioxidative repair systems can be expected to minimize DNA lesions caused by mercury. In addition, the results suggest that the determination of the urinary 8-OHdG content is a use ful way for evaluating in vivo oxidativeDNA damage in the Hg-exposed populations.

biomarkerDNA damagemercury exposure

Shu-Chu LIU、Yin-Jing HSIEH

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Chunying CHEN@Key Laboratory of Nuclear Analysis Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China--Yu-Feng LI@Key Laboratory of Nuclear Analysis Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China--Liya Qu@Guizhou Research and Designing Institute of Environmental Sciences, Guiyang 550004, P. R. China--Mei Li@Guizhou Research and Designing Institute of Environmental Sciences, Guiyang 550004, P. R. China--Bai LI@Key L

Biogeochemistry of Trace Elements: Environmental Protection, Remediation and Human Health international Symposium

Biogeochemistry of Trace Elements: Environmental Protection, Remediation and Human Health

464-465

2007