Automated High-throughput SNP Detection using Magnetic Composite Particles

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原文链接

NETL

外文摘要：A high-throughput automated approach for single nucleotide polymorphisms (SNPs) genotyping using solid-phase PCR on magnetic composite particles (MCPs) was developed. Biotin-labeled reverse primer was captured by streptavidin magnetic composite particles (SA-MCPs), PCR products were directly amplified on the surface of SA-MCPs in a 96-well plate. The ssDNA amplified on MCPs was hybridized with a pair of dual-color probes, and then genotype of each sample can be simultaneously identified by scanning the microarray printed with the denatured fluorescent probes. All the procedures were performed on an automated workstation equipped with a magnetic separation device. The M235T polymorphism in angiotensinogen (AGT) gene from 160 samples was successfully interrogated by this method. With automated operation and microarray based “read-out” technology, this approach will be a novel, simple and labor-saving method for large-scale SNP detection.

外文关键词：

magnetic composite particlessolid-phase PCRSNP genotypingautomated workstation

作者：

Nongyue He、Song Li、Hongna Liu

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作者单位：

State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering.Southeast University, Nanjing, 210096, China

会议地点：

苏州

出版时间：

2007