Influence of B Ring Structure on Cell Cycle Inhibitory Activities of Flavonols in a Human Oesophageal Adenocarcinoma Cell Line (OE33)

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外文摘要：The effects of three structurally related flavonols (kaempferol， quercetin， myricetin) on anti-proliferation and cell cycle arrest in human oesophageal adenocarcinoma OE33 cells were determined and compared by MTT assay and flow cytometry analysis。 The results showed that three flavonols not only inhibited proliferation but also induced G2/M arrest in OE33 cells。 Moreover， G2/M arrest-inducing activity of quercetin (with two hydroxyl groups in the B ring) was better than that of kaempferol (with one hydroxyl group in the B ring) and myricetin (with three hydroxyl groups in the B ring)。 It was further corroborated by comparing G2/M arrest-inducing activity of two flavones (apigenin and luteolin) whose B rings were same to kaempferol and quercetin， respectively。 Additionally， the results of real-time RT-PCR and Western-blot analysis showed that the expressions of GADD45beta and 14-3-3sigma were upregulated but the expression of cyclin B1 was downregulated by flavonols at the mRNA and the protein levels。 It is suggested that the influence of B ring structure on cell cycle inhibitory activities of flavonols might result from the regulatory expression of these genes at the mRNA and protein levels。

外文关键词：

cancercellular biophysicsdrugsmacromoleculesproteinstumoursB ring structureGADD45betaMTT assayWestern-blot analysisanti-cell proliferationanticancer activitycell cycle inhibitory activityflavonolflow cytometry analysishuman oesophageal adenocarcinoma cell linekaempferolmRNAmyricetinprotein levelquercetinreal-time RT-PCR

作者：

Qiang Zhang、Xinhuai Zhao

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作者单位：

Key Lab. of Diary Sci. of Minist. of Educ., Northeast Agric. Univ., Harbin, China

会议地点：

Beijing(CN)

会议母体文献：

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009

页码：

1-4

出版时间：

2009

DOI：

10.1109/ICBBE.2009.5162954