Analysis of Bacterial Community Structure in Vermifiltration for Sludge Reduction by Nested PCR-DGGE Method

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外文摘要：Vermiflltration was a innovative process to sludge reduction。 The relationship of earthworm and bacterial community was not conclusive in the treatment。 The techniques of polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community structure in vermifiltration column in the paper。 Results showed bacterial diversity index (H) in the middle layer was 1。87 and 3。19， and subsequently in the lower layer was 1。24 and 1。58。 H in the upper layer was lowest (0。67 and 1。07)。 11 bacterial organism were identified in the columns， demonstrated that richness and complexity of bacterial ecology。 The important factors to determine bacterial community structure in vermifiltration was likely to be earthworm predation and cast， rather than bacterial community of raw sludge。 Earthworm biomass reduced gradually in the depth distribution。 The possible reasons were possible to be food sources and dissolved oxygen。

外文关键词：

DNAbiochemistrybiological techniqueselectrophoresisenzymesmicroorganismspattern clusteringsludge treatmentstatistical analysiswastewater treatmentPCR-DGGE methodbacterial community structurebacterial diversity indexdenaturing gradient gel electrophoresispolymerase chain reactionsludge reductionvermifiltration

作者：

Jian Yang、Xiaowei Li、Limin Zhao、Dehan Deng、Danghao Yi、Meiyan Xing

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作者单位：

State Key Lab. of Pollution Control & Resource Reuse, Tongji Univ., Shanghai, China

会议地点：

Beijing(CN)

会议母体文献：

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009

页码：

1-4

出版时间：

2009

DOI：

10.1109/ICBBE.2009.5162343