首页|EFFECT OF GENOTYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION IN SUGARCANE
EFFECT OF GENOTYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION IN SUGARCANE
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NETL
Young leaf bit explants of three sugarcane species (S。 officinanim。 S。 barberi。 S。 sinense)。 one intergeneric hybrid (Saccharum x Zeal and five varieties (Co 86032， Co 99004， Co 94012， Co 1148 and CoJ 64) was asepticaUy inoculated on MS medium containing different concentration of 2， 4-D (0， 1。0， 2。0， 3。0， 4。0 mg/l)for callus induction。 Maximum of 95% callus induction was observed in the variety Co 99004 and Saccharum x Zea showed lowest callus induction (40%)。 The actively growing calli were transferred to MS medium supplemented with different concentrations of 6-Benzyl Amino Purine (BAP)， kinetin and combination of BAP and kinetinfor shoot regeneration。 Most of the genotypes showed maximum shoot regeneration in MS medium supplemented with a combination of BAP and kinetin (0。5 mg/l each)。 S。 officinarum and Co 86032 showed maximum number of regenerated shoots in medium supplemented with 0。5 mg/lBAP + 0。5 mg/l kinetin and 1 mg/l kinetin respectively。 From the study it was concluded that callus induction and shoot regeneration is significantly affected by genotype and the growth regulators。
NETL
