Isolation and Identification of Leaf Blight Pathogen from Camellia oleifera

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外文摘要：Leaf blight of Camellia oleifera was one of the major diseases in Hunan's Camellia producing areas.The fungal pathogen infected leaves of the host plants and caused leaves drop, resulting in earlier defoliation and new treetops death.Filamentous fungal strain (No.CSUFT070160A) was isolated from the leaves of Camellia oleifera with the leaf blight disease.We observed morphological characteristics and analyzed the sequences of rDNA ITS from the strain, and concluded that the rDNA ITS of the strain was 516bp.By comparing with sequences in the GeneBank, the strain CSUFT070160A have the highest homologous to Pestalotiopsis microspora, more than 99%.The molecule phylogenetic tree indicated that the CSUFT070160A and the DQ000995 wre in the same branch with high confidence level.And combined with morphological characteristics we can confirmed that the CSUFT070160A strain belonged to Pestalotiopsis microspora.The research results lay a foundation for preventing and control the pathogens of Camellia oleifera leaf blight.

外文关键词：

pathogenicity testmorphologyrDNAcamellia oleiferaleaf blight

作者：

ZHOU Guoying、SONG Guangtao、LIU Junang、LI He

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作者单位：

Biotechnology Core Facilities Central South University of Forestry and Technology Changsha,Hunan,China

College of Forestry Central South University of Forestry and Technology Changsha,Hunan,China

会议名称：

The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议）论文集

会议地点：

Beijing(CN)

会议母体文献：

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009

页码：

1-3

出版时间：

2009