首页|ESTIMATION OP CARBOHYDRATES BY HPLC FOR DEVELOPMENT OF MOLECULAR MARKER TOOL

ESTIMATION OP CARBOHYDRATES BY HPLC FOR DEVELOPMENT OF MOLECULAR MARKER TOOL

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The objective of the study was to determine changes in glucose， fructose and sucrose content in the storage sink tissues of various co-cultivars sugarcane varieties at different time intervals and its correlation between total soluble solids with carbohydrates。 We selected three high sugar content and three low sugar content sugarcane genotypes。 Mature stalks were collected and crushed for juice analysis by conventional (Brix-pol) method and high performance liquid chromatography (HPLC) used for estimation of glucose， fructose and sucrose。 The components were eluted with acetonitrile and water (75 : 25) mobile phase at a flow rate of 0。8 ml/minfrom an Ultra Amino 3。0 um column (150× 4。6 mm- Restek， (USA)。 HPLC was performed with isocratic system and Refractive Index (RI) detector on waters instrument and the chromatographic data were collected with Empower-2 software。 Generally， during maturity of sugarcane， glucose and fructose content decreased， while that of sucrose and brix increased。 On comparison qfbrix with carbohydrate estimated by HPLC， a positive correlation was observed for sucrose (r = 0。890) and negative correlation were found for glucose and fructose (r = 0。742 and r = 0。701， respectively)。 It was found that a change in biochemical level also reveals the morphological and genetic variation in sugarcane genotypes。 This data reinforced the effectiveness of carbohydrate profiling approach to uncover molecular markers identification and determination for sugar related traits in sugarcane cultivars。

sugarcaneHPLCglucosefructosesucrosebrix and molecular markers

S.B. Kalwade、S.P. Takle、P.G. Kawar、R.M. Devarumath、K.H.Babu、S. V. Patil

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Vasantdada Sugar Institute, Manjari (Bk.), Tal. Haveli, Pune, 412307, India

Joint convention of the Sugar Technologists' Association of India & the Deccan Sugar Technologists' Association

Pune(IN)

Proceedings of the 10th joint convention of the Sugar Technologists' Association of India (STAI) & the Deccan Sugar Technologists' Association (India)

p.b26-b35

2011