Multilayered phantoms with tunable optical properties for a better understanding of light/tissue interactions

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原文链接

NETL
Spie-Int Soc Optical Engineering

外文摘要：Light/tissue interactions like diffuse reflectance， endogenous fluorescence and Raman scattering are powerful means for providing skin diagnosis。 Instrument calibration is an important step。 We thus developed multilayered phantoms for calibration of optical systems。 These phantoms mimic the optical properties of biological tissues such as skin。 Our final objective is to better understand light/tissue interactions especially in the case of confocal Raman spectroscopy。 The phantom preparation procedure is described including the employed method to obtain a stratified object。 PDMS was chosen as the bulk material。 TiO2 was used as light scattering agent。 Dye and ink were adopted to mimic， respectively， oxy-hemoglobin and melanin absorption spectra。 By varying the amount of the incorporated components， we created a material with tunable optical properties。 Monolayer and multilayered phantoms were designed to allow several characterization methods。 Among them， we can name: X-ray tomography for structural information; Diffuse Reflectance Spectroscopy (DRS) with a homemade fibered bundle system for optical characterization; and Raman depth profiling with a commercial confocal Raman microscope for structural information and for our final objective。 For each technique， the obtained results are presented and correlated when possible。 A few words are said on our final objective。 Raman depth profiles of the multilayered phantoms are distorted by elastic scattering。 The signal attenuation through each single layer is directly dependent on its own scattering property。 Therefore， determining the optical properties， obtained here with DRS， is crucial to correct properly Raman depth profiles。 Thus， it would be permitted to consider quantitative studies on skin for drug permeation follow-up or hydration assessment for instance。

外文关键词：

Optical phantomPhantom characterizationoptical propertiesSkin mimicking phantoms

作者：

Blandine Roig、Anne Koenig、Francois Perraut、Olivier Piot、Severine Vignoud、Jonathan Lavaud、Michel Manfait、Jean-Marc Dinten

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作者单位：

CEA, Leti, MINATEC Campus, 17 rue des Martyrs, F38054 GRENOBLE, Cedex 9, FRANCE

MeDIAN Biophotonique et Technologies pour la Sante, UMR CNRS 7369 MEDyC, UFR Pharmacie, 51 rue Cognacq-Jay, 51096 Reims Cedex, France

Institut Albert Bonniot - INSERM U823 - Equipe 5, BP170 38042 Grenoble cedex 9

会议名称：

Conference on design and performance validation of phantoms used in conjunction with optical measurement of tissue

会议地点：

San Francisco, CA(US)

会议母体文献：

Design and performance validation of phantoms used in conjunction with optical measurement of tissue VII

页码：

93250B.1-93250B.6

出版时间：

2015

DOI：

10.1117/12.2077212