This paper is estimated the survival ratio by the experiments and the calculations by the sterilization model under the high electric field pulse. The sample is NaCl aqueous solution containing Escherichia coli. We applied high voltage pulse (pulse width: 5 μs or 160 μs) to the sample. As results, the effect of the pulse width on the survival ratio of E. coli is showed. The calculated values of survival ratio in the sterilization mechanism with considering pulse width agreed with our experimental results.