A CRISPR/CAS9 BASED ENGINEERING TOOL TO ACTIVATE EXPRESSION OF MULTIPLE GEN INDIVIDUALLY OR IN ANY SPECIFIC COMBINATION

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原文链接

NETL

外文摘要：Engineering of cells by overexpression or knock-down/out of individual genes has demonstrated that in most cases the manipulation of single genes is not sufficient to alter a cellular phenotype. Rather, multiple genes involved in a pathway need to be manipulated. Especially in mammalian cells such as CHO, where clonal variation is large, it has been difficult to unequivocally assess whether the observed change in phenotype is due to such clonal variation or the engineered gene. This can in part be overcome by testing multiple subclones, however, once it comes to engineering multiple genes and combinations thereof, the required workload quickly becomes prohibitive. We here present a simple technology for successive and/or specific activation of multiple genes integrated into a single genomic locus, which presents a potential solution to this problem.

外文关键词：

CRISPR/Cas9CHOpathway engineering

作者：

Peter Eisenhut、Gerald Klanert、Marcus Weinguny、Daniel Ivansson、Nicole Borth

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作者单位：

Austrian Centre of Industrial Biotechnology, Vienna

GE Healthcare, Uppsala

BOKU University and Austrian Centre of Industrial Biotechnology, Vienna

会议名称：

Conference on biochemical and molecular engineering XX

会议地点：

Newport Beach(US)

会议母体文献：

Biochemical and molecular engineering XX: the next generation of biochemical engineering: from nanoscale to industrial scale

页码：

59-59

出版时间：

2017