小胸鳖甲6个几丁质酶基因的克隆、序列分析及低温表达模式研究

扫码查看

原文链接

NETL

中文摘要：Chitinase is responsible for chitin metabolism in a wide range of organisms. However, currentknowledge on insect chitinase and possible functions in relation to low temperature stress is very limited. Sixchitinase genes from cold treated desert beetle Microc}era puuctipeuuis obtained by RNA-seq technology werecharacterized, and their expression patterns in different tissues and in response to cold were investigated. Multiplesequence alignment was carried out using Clusta1W1.81 and Phylogenetic trees were generated by MEGAS. Theexpression patterns were studied by quantitative real-time PCR. These genes were assigned to three differentchitinase groups. Almost all of them were highly expressed in midgut, some are expressed in fat body or hindgut.-40C had stronger effect than 40C in inducing chitinase expression. Chitinase genes from M. puuctipeuuis exist in abig family. The tissue specific and cold inducible expressions suggest that the chitinases may have diversefunctions and play roles in insect cold adaptation.

作者：

Xueying Lu、Jieqiong Li、Xiaoning Liu、Xiaobo Li、Ji Ma

展开 >

作者单位：

Key Laboratory of Xinjiang Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi,China

Key Laboratory of Chemistry of Plant Resources in Arid Regions,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi,China

关键词：

小胸鳖甲几丁质酶基因序列分析低温表达模式

主办单位：

新疆动物学会

会议名称：

新疆动物学会2014年年会暨学术研讨会

会议时间：

2014-10-16

会议地点：

乌鲁木齐

会议母体文献：

新疆动物学会2014年年会暨学术研讨会论文集

页码：

240-252

出版时间：

2014