首页|ALLEVIATION OF COPPER-INDUCED SEED GERMINATION INHIBITION AND OXIDATIVE DAMAGE IN ALFALFA BY METHANE-RICH WATER
ALLEVIATION OF COPPER-INDUCED SEED GERMINATION INHIBITION AND OXIDATIVE DAMAGE IN ALFALFA BY METHANE-RICH WATER
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Background and aims:Recently,researchers discovered the protective effects of methane(CH4)in animals against oxidative stress.But,its possible physiological roles in plants are largely unknown.Here,we evaluate the role of copper(Cu)-stressed increase in CH4production from germinating alfalfa seeds. Methods:We adopted physiological,histochemical and biochemical approaches to study the beneficial role of CH4in the alleviation of Cu toxicity in germinating alfalfa seeds. Results:Our results showed that endogenous production of CH4was significantly increased in copper(Cu)-stressed alfalfa seeds as compared to control.Further,results showed that the pretreatment with30％saturation of MRW significantly alleviated the inhibition of seed germination and seedling growth,and oxidative stress induced by Cu stress,the latter of which were supported by the decrease of TBARS content and reduction of Cu-induced loss of plasma membrane integrity in MRW-pretreated alfalfa seedlings.Meanwhile,MRW could increaseα/βamylase activities and sugar contents,both of which were consistent with the alleviation of seed germination.Cu accumulation was also obviously decreased by MRW.In comparison with Cu stress alone,pretreatment with30％saturation of MRW significantly decreased the Cu-induced stimulation of POD and P5CS activities,and as well proline accumulation,While,the inhibition of activities of ascorbate peroxidase(APX),superoxide dismutase(SOD),catalase(CAT)and corresponding transcripts(APX1/2,PDH,Cu/Zn SOD and Mn-SOD),were eliminated. Conclusions:Together,our results provide a new evidence for the potential beneficial role of MRW in the alleviation of Cu toxicity during alfalfa seed germination.
Muhammad Kaleem Samma
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Medicagosativa Methane-rich water Copper toxicity Oxidative damage Seed germination
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