期刊,Cryobiology 2021年101卷期_国家学术搜索

Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis

Arshad, UsmanSagheer, MasroorGonzalez-Silvestry, Fatima B.Hassan, Mubbashar...

11页

查看更多>>摘要：Objectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3473) survival of Bos taurus embryos. The literature was systematically reviewed and data from 40 manuscripts including 78 experiments, and comprising 183 treatment means, were used for the analyses. The in-vitro parameters included rates of re-expansion, hatching, and survival of blastocysts either at 24 h or 72 h post-thawing/warming and total number (TN) of embryonic cells, whereas in-vivo parameters evaluated pregnancy rate between 35 and 60 d post embryo transfer (ET). Mixed models were fitted using MIXED and GLIMMIX procedures of SAS. Additionally, classical meta-analytical statistics were also fitted using METAN and METAREG procedures of STATA. The final models included the fixed effects of methods of cryopreservation and random effects of the experiment. Rates (LSM +/- SEM) of re-expansion (0.36 +/- 0.07 vs. 0.48 +/- 0.08), hatching (0.25 +/- 0.05 vs. 0.42 +/- 0.07), and survival (0.57 +/- 0.09 vs. 0.76 +/- 0.07) at 72 h post-thawing/warming were lower (P < 0.05) in SF than VF, respectively. The TN of embryonic cells (96.89 +/- 7.15 vs. 117.83 +/- 7.15) remained lower (P 0.05) in SF than VF, however, the relative risk (RR) of pregnancy rate post ET remained similar (RR = 1.0, CI = 0.8-1.2; P 0.05) between both methods. Collectively, VF technique has a short-term protective effect against cryodamage of preimplantation embryos, however, it might be dysregulating genes involved in pregnancy success post ET in cows.

原文链接:

NSTL
Elsevier

Vitrification improves in-vitro embryonic survival in Bos taurus embryos without increasing pregnancy rate post embryo transfer when compared to slow-freezing: A systematic meta-analysis

Arshad, UsmanSagheer, MasroorGonzalez-Silvestry, Fatima B.Hassan, Mubbashar...

11页

查看更多>>摘要：Objectives were to use meta-analytic approaches to compare slow-freezing (SF) and vitrification (VF) methods of cryopreservation on in-vitro (n = 12,211) and in-vivo (n = 3473) survival of Bos taurus embryos. The literature was systematically reviewed and data from 40 manuscripts including 78 experiments, and comprising 183 treatment means, were used for the analyses. The in-vitro parameters included rates of re-expansion, hatching, and survival of blastocysts either at 24 h or 72 h post-thawing/warming and total number (TN) of embryonic cells, whereas in-vivo parameters evaluated pregnancy rate between 35 and 60 d post embryo transfer (ET). Mixed models were fitted using MIXED and GLIMMIX procedures of SAS. Additionally, classical meta-analytical statistics were also fitted using METAN and METAREG procedures of STATA. The final models included the fixed effects of methods of cryopreservation and random effects of the experiment. Rates (LSM +/- SEM) of re-expansion (0.36 +/- 0.07 vs. 0.48 +/- 0.08), hatching (0.25 +/- 0.05 vs. 0.42 +/- 0.07), and survival (0.57 +/- 0.09 vs. 0.76 +/- 0.07) at 72 h post-thawing/warming were lower (P < 0.05) in SF than VF, respectively. The TN of embryonic cells (96.89 +/- 7.15 vs. 117.83 +/- 7.15) remained lower (P 0.05) in SF than VF, however, the relative risk (RR) of pregnancy rate post ET remained similar (RR = 1.0, CI = 0.8-1.2; P 0.05) between both methods. Collectively, VF technique has a short-term protective effect against cryodamage of preimplantation embryos, however, it might be dysregulating genes involved in pregnancy success post ET in cows.

原文链接:

NSTL
Elsevier

The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in Turkey semen during liquid storage at 5 degrees C

Izanloo, HasanSoleimanzadeh, AliBucak, Mustafa NumanImani, Mehdi...

8页

查看更多>>摘要：Since turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 degrees C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. A glucose-based extender was used to dilute the pooled semen. It was divided into seven equal parts with varying levels of glutathione [0.5, 1 and 2 mM), trehalose [50, 75 and 100] and control [extender without antioxidant]. Subsequently, the divided semen samples were stored at 5 degrees C for 72 h. Several sperm parameters such as motility and motion parameters, plasma membrane integrity (PMI), plasma membrane functionality, DNA integrity, and oxidative parameters were assessed following storage for 0, 24, 48, and 72 h. The obtained results indicated an improvement in the plasma membrane functionality and DNA integrity, along with the percentages of PMI in GSH-2 mM group in comparison to the control group following storage at 5 degrees C for 72 h (P < 0.05). It is also notable that the 2 and 1 mM concentrations of GSH increased the spermatozoa motility and motion parameters in comparison to the control group, respectively (P < 0.05). The study results indicated that GSH-2, 1 mM and trehalose- 100 mM concentrations reduced lipid peroxidase levels and increased total antioxidant activity, catalase, superoxide dismutase, and glutathione peroxidase in comparison to the control group (P < 0.05). Our study's data show that improvement of semen parameters and oxidative stress parameters of turkey semen can be improved by glutathione at 2 and 1 mM and trehalose at 75 mM while storing it 5 degrees C.

原文链接:

NSTL
Elsevier

The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in Turkey semen during liquid storage at 5 degrees C

Izanloo, HasanSoleimanzadeh, AliBucak, Mustafa NumanImani, Mehdi...

8页

查看更多>>摘要：Since turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 degrees C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. A glucose-based extender was used to dilute the pooled semen. It was divided into seven equal parts with varying levels of glutathione [0.5, 1 and 2 mM), trehalose [50, 75 and 100] and control [extender without antioxidant]. Subsequently, the divided semen samples were stored at 5 degrees C for 72 h. Several sperm parameters such as motility and motion parameters, plasma membrane integrity (PMI), plasma membrane functionality, DNA integrity, and oxidative parameters were assessed following storage for 0, 24, 48, and 72 h. The obtained results indicated an improvement in the plasma membrane functionality and DNA integrity, along with the percentages of PMI in GSH-2 mM group in comparison to the control group following storage at 5 degrees C for 72 h (P < 0.05). It is also notable that the 2 and 1 mM concentrations of GSH increased the spermatozoa motility and motion parameters in comparison to the control group, respectively (P < 0.05). The study results indicated that GSH-2, 1 mM and trehalose- 100 mM concentrations reduced lipid peroxidase levels and increased total antioxidant activity, catalase, superoxide dismutase, and glutathione peroxidase in comparison to the control group (P < 0.05). Our study's data show that improvement of semen parameters and oxidative stress parameters of turkey semen can be improved by glutathione at 2 and 1 mM and trehalose at 75 mM while storing it 5 degrees C.

原文链接:

NSTL
Elsevier

Toxicity of cryoprotective agents to semen from two closely related snake species: The endangered Louisiana pinesnake (Pituophis ruthveni) and bullsnake (Pituophis cantenifer)

Sandfoss, Mark R.Whittington, Oliver M.Reichling, SteveRoberts, Beth M....

8页

查看更多>>摘要：Cryopreservation of sperm is an important tool for the conservation of threatened species. Many species of reptile are under considerable threat of extinction and there has been limited investigation of sperm cryopreservation in this taxonomic group. We performed a comparative test of toxicity to sperm of six commonly used cryoprotective agents (CPAs) at three concentrations (5%, 10%, 20%) from the Louisiana pinesnake, Pituophis ruthveni (n = 11), and the closely related bullsnake, Pituophis cantenifer (n = 8). Our objective was to determine the general toxicity of CPAs for cryopreservation in snakes and the cryoprotective ability of CPAs for sperm from the endangered Louisiana pinesnake. We conducted three experiments to: 1) evaluate the short-term in vitro toxicity of common CPAs in two closely related snake species, 2) determine the effectiveness of cryoprotectants for freezing and thawing semen in the Louisiana pinesnake, and 3) test the possible reduction in toxic effects of individual CPAs on semen of the Louisiana pinesnake by combining two of them. We used measures of motility including total motility, forward motility, and forward progressive motility index to characterize toxic effects and cryoprotective ability of each CPA. The results of our three experiments provide several important findings: 1) sperm of the bullsnake and Louisiana pinesnake responded differently to CPAs, 2) few CPAs provided any cryoprotection, as measured by percent recovered motility, in Louisiana pinesnakes, and 3) using mixtures of CPAs did not reduce toxicity relative to the best performing CPA on its own. Motility was best maintained at a concentration of 5% for CPAs tested; however, cryoprotection was best achieved with glycerol at 20% followed by DMA and DMF at 10%. These results provide further insight into the challenges faced by researchers attempting to cryopreserve sperm from snakes. Further comparative studies are required to determine the generality of cryopreservation methods in reptiles and suggest caution should be taken when developing cryopreservation protocols across species, particularly in snakes. All CPAs tested in this study were permeating CPAs and showed a significant acute toxic effect on motility at concentrations that provided cryoprotection. Future work in snakes might consider additional avenues of cryoprotection and combinations of multiple approaches.

原文链接:

NSTL
Elsevier

Toxicity of cryoprotective agents to semen from two closely related snake species: The endangered Louisiana pinesnake (Pituophis ruthveni) and bullsnake (Pituophis cantenifer)

Sandfoss, Mark R.Whittington, Oliver M.Reichling, SteveRoberts, Beth M....

8页

查看更多>>摘要：Cryopreservation of sperm is an important tool for the conservation of threatened species. Many species of reptile are under considerable threat of extinction and there has been limited investigation of sperm cryopreservation in this taxonomic group. We performed a comparative test of toxicity to sperm of six commonly used cryoprotective agents (CPAs) at three concentrations (5%, 10%, 20%) from the Louisiana pinesnake, Pituophis ruthveni (n = 11), and the closely related bullsnake, Pituophis cantenifer (n = 8). Our objective was to determine the general toxicity of CPAs for cryopreservation in snakes and the cryoprotective ability of CPAs for sperm from the endangered Louisiana pinesnake. We conducted three experiments to: 1) evaluate the short-term in vitro toxicity of common CPAs in two closely related snake species, 2) determine the effectiveness of cryoprotectants for freezing and thawing semen in the Louisiana pinesnake, and 3) test the possible reduction in toxic effects of individual CPAs on semen of the Louisiana pinesnake by combining two of them. We used measures of motility including total motility, forward motility, and forward progressive motility index to characterize toxic effects and cryoprotective ability of each CPA. The results of our three experiments provide several important findings: 1) sperm of the bullsnake and Louisiana pinesnake responded differently to CPAs, 2) few CPAs provided any cryoprotection, as measured by percent recovered motility, in Louisiana pinesnakes, and 3) using mixtures of CPAs did not reduce toxicity relative to the best performing CPA on its own. Motility was best maintained at a concentration of 5% for CPAs tested; however, cryoprotection was best achieved with glycerol at 20% followed by DMA and DMF at 10%. These results provide further insight into the challenges faced by researchers attempting to cryopreserve sperm from snakes. Further comparative studies are required to determine the generality of cryopreservation methods in reptiles and suggest caution should be taken when developing cryopreservation protocols across species, particularly in snakes. All CPAs tested in this study were permeating CPAs and showed a significant acute toxic effect on motility at concentrations that provided cryoprotection. Future work in snakes might consider additional avenues of cryoprotection and combinations of multiple approaches.

原文链接:

NSTL
Elsevier

Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation

Green, Stuart R.Storey, Kenneth B.

10页

查看更多>>摘要：Hibernation is a metabolic/physiological strategy employed by many mammals to cope with periods when energy usage is greater than its input. Animals undergoing hibernation need to greatly reduce their metabolic rate and reshape their catabolic processes to survive on stored triglycerides. Citrate synthase (CS) is one of only two irreversible steps in the citric acid cycle (CAC) and forms an important regulatory checkpoint that gates the entry of acetyl-CoA formed in glycolysis or fatty acid catabolism into this critical central metabolic hub. This study investigated the regulation of citrate synthase in the muscle tissue of a small mammalian hibernator through comparison of functional and structural properties. The results demonstrated a significant decrease in the Vmax of purified torpid CS compared to the control euthermic enzyme (1.2-1.7 fold greater in the control) that was evident over a wide range of temperatures (8, 22 and 37 degrees C) that are encountered by the enzyme in hibernation. This was also reflected in the specific activity of the enzyme in crude muscle protein extracts. Analyzing the purified CS through immunoblotting demonstrated that the enzyme contained noticeably less lysine succinylation in the torpid state (about 50% of euthermic levels) and this was correlated with an increase in total levels of SIRT5, the enzyme responsible for mediating desuccinylation in the mitochondria (2.2 fold increase). Taken together, the results of this study support the idea that CS is inhibited during hibernation in the ground squirrel skeletal muscle and that this alteration could be mediated by decreases in succinylation.

原文链接:

NSTL
Elsevier

Skeletal muscle of torpid Richardson's ground squirrels (Urocitellus richardsonii) exhibits a less active form of citrate synthase associated with lowered lysine succinylation

Green, Stuart R.Storey, Kenneth B.

10页

查看更多>>摘要：Hibernation is a metabolic/physiological strategy employed by many mammals to cope with periods when energy usage is greater than its input. Animals undergoing hibernation need to greatly reduce their metabolic rate and reshape their catabolic processes to survive on stored triglycerides. Citrate synthase (CS) is one of only two irreversible steps in the citric acid cycle (CAC) and forms an important regulatory checkpoint that gates the entry of acetyl-CoA formed in glycolysis or fatty acid catabolism into this critical central metabolic hub. This study investigated the regulation of citrate synthase in the muscle tissue of a small mammalian hibernator through comparison of functional and structural properties. The results demonstrated a significant decrease in the Vmax of purified torpid CS compared to the control euthermic enzyme (1.2-1.7 fold greater in the control) that was evident over a wide range of temperatures (8, 22 and 37 degrees C) that are encountered by the enzyme in hibernation. This was also reflected in the specific activity of the enzyme in crude muscle protein extracts. Analyzing the purified CS through immunoblotting demonstrated that the enzyme contained noticeably less lysine succinylation in the torpid state (about 50% of euthermic levels) and this was correlated with an increase in total levels of SIRT5, the enzyme responsible for mediating desuccinylation in the mitochondria (2.2 fold increase). Taken together, the results of this study support the idea that CS is inhibited during hibernation in the ground squirrel skeletal muscle and that this alteration could be mediated by decreases in succinylation.

原文链接:

NSTL
Elsevier

Survival of thermophilic fungi in various preservation methods: A comparative study

Berikten, Derya

6页

查看更多>>摘要：Thermophilic fungi have several biotechnological and industrial applications such as thermostable enzyme production, biodegradation, and tobacco processing, etc. Thermophilic fungi cannot survive at temperatures below 20 degrees C. Owing to their inability to grow at low temperatures, they are not stable, so stocking is very difficult. Although a large number of different storage methods are available and described, no method can be universally applied to all fungi. Thermophilic fungi present "heat-loving" characteristics, and therefore a new challenge for its preservation and there is no universal protocol for the preservation of thermophilic fungi. The aim of this study was to evaluate the viability, contamination and stability of thermophilic fungi stored under different preservation methods. In this work, 25 thermophilic fungal isolates of species Thermomyces thermophilus, Rhizomucor pusillus, Trichocladium griseum, Melanocarpus albomyces, Malbranchea cinnamomea, Thermothelomyces thermophilus, Thermothelomyces hinnuleus, Thermothielavioides terrestris, Mycothermus thermophilus, Humicola insolens maintained constant sub-culturing at room temperature, +4 degrees C and +20 degrees C, lyophilization at +4 degrees C, freezing at -20 degrees C, freezing block at -20 degrees C and a new technique liquid preservation at room temperature for the periods ranging 5 years. We evaluated the effect of preservation methods by sub-culturing onto either sabouraud dextrose agar (SDA) or yeast extract soluble starch agar (YpSs) on growth, production and viability of spores and macro- and micromorphology. In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 degrees C method and lyophilization were better methods for long-term preservation up to 5 years.

原文链接:

NSTL
Elsevier

Survival of thermophilic fungi in various preservation methods: A comparative study

Berikten, Derya

6页

查看更多>>摘要：Thermophilic fungi have several biotechnological and industrial applications such as thermostable enzyme production, biodegradation, and tobacco processing, etc. Thermophilic fungi cannot survive at temperatures below 20 degrees C. Owing to their inability to grow at low temperatures, they are not stable, so stocking is very difficult. Although a large number of different storage methods are available and described, no method can be universally applied to all fungi. Thermophilic fungi present "heat-loving" characteristics, and therefore a new challenge for its preservation and there is no universal protocol for the preservation of thermophilic fungi. The aim of this study was to evaluate the viability, contamination and stability of thermophilic fungi stored under different preservation methods. In this work, 25 thermophilic fungal isolates of species Thermomyces thermophilus, Rhizomucor pusillus, Trichocladium griseum, Melanocarpus albomyces, Malbranchea cinnamomea, Thermothelomyces thermophilus, Thermothelomyces hinnuleus, Thermothielavioides terrestris, Mycothermus thermophilus, Humicola insolens maintained constant sub-culturing at room temperature, +4 degrees C and +20 degrees C, lyophilization at +4 degrees C, freezing at -20 degrees C, freezing block at -20 degrees C and a new technique liquid preservation at room temperature for the periods ranging 5 years. We evaluated the effect of preservation methods by sub-culturing onto either sabouraud dextrose agar (SDA) or yeast extract soluble starch agar (YpSs) on growth, production and viability of spores and macro- and micromorphology. In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 degrees C method and lyophilization were better methods for long-term preservation up to 5 years.

原文链接:

NSTL
Elsevier