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Clinica chimica acta
Elsevier
Clinica chimica acta

Elsevier

0009-8981

Clinica chimica acta/Journal Clinica chimica actaSCIISTPIC
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    National external quality assessment and direct method comparison reflect crucial deviations of Procalcitonin measurements in Germany

    Masetto, ThomasEidizadeh, AbassPeter, ChristophGrimmler, Matthias...
    9页
    查看更多>>摘要：Background and aims: Sepsis is a major concern worldwide, affecting 49 million individuals and being related to 11 million deaths. Its fast diagnosis is the key factor to guarantee a positive prognosis. Procalcitonin (PCT) has emerged as one powerful biomarker to early diagnose sepsis and for monitoring of antibiotic treatment. However, its clinical utility is jeopardized by missing standardisation. Materials and methods: Here we present a 1-year follow-up of the External Quality Assessment (EQA) in Germany, depicting substantial discrepancies among manufacturers and the used assay technology of current PCT measurements. A direct method comparison on two immunoassays (Abbott vs. DiaSys) on a set of 135 routine samples was used to analyse the causes of observed deviations. Results: All BRAHMS-licensed manufacturers (Thermo, Roche, Abbott, Siemens, Biomerieux), the Beckman and DiaSys immunoassays as well as all assay types (fluorescence, luminescence, PETIA) reveal substantial recovery differences between each other. However, upon a non-linear re-standardization of calibrators, the two directly compared methods (Abbott, DiaSys) are well interchangeable. Conclusion: This work demonstrates the heterogenic situation of PCT measurements in Germany among manufacturers and all methods. By introducing dedicated correction factors, comparable results of PCT can be achieved. This work also strengthens the inevitability of calibrator traceability and higher metrological reference materials on PCT.
      原文链接:
    • NSTL
    • Elsevier

    alpha-Synuclein in Parkinson's disease and advances in detection

    Gu, XuanWang, XiaoyingChen, Rong
    11页
    查看更多>>摘要：Parkinson's disease (PD) is a threatening neurodegenerative disorder that seriously affects patients' life quality. Substantial evidence links the overexpression and abnormal aggregation of alpha-synuclein (alpha-Syn) to PD. alpha-Syn has been identified as a characteristic biomarker of PD, which indicates its great value of diagnosis and designing effective therapeutic strategy. This article systematically summarizes the pathogenic process of alpha-Syn based on recent researches, outlines and compares commonly used analysis and detection technologies of alpha-Syn. Specifically, the detection of alpha-Syn by new electrochemical, photochemical, and crystal biosensors is mainly examined. Furthermore, the speculation of future study orientation is discussed, which provides reference for the further research and application of alpha-Syn as biomarker.
      原文链接:
    • NSTL
    • Elsevier

    Decreased serum CXCL12/SDF-1 concentrations may reflect disease severity of non-traumatic osteonecrosis of femoral head

    Zheng, Shao-WeiSun, Chun-HanWen, Zhi-JiaLiu, Wei-Le...
    9页
    查看更多>>摘要：Objective: The current study was performed to investigate the potential association of serum CXCL12 with disease severity in non-traumatic ONFH. Methods: This study enrolled 182 patients with non-traumatic ONFH and 182 age-and gender-matched healthy controls. The CXCL12 levels in serum were measured by enzyme-linked immunosorbent assay. Meanwhile, serum levels of procollagen type I (PINP) and Interleukin-33(IL-33) were also detected. The radiographic severity was determined by FICAT grade. Clinical severity was evaluated by visual analogue scale (VAS), Harris Hip Score (HHS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Among the non traumatic ONFH, 90 patients ONFH received total hip arthroplasty, the localization and expression of the CXCL12 protein and mRNA were detected by immunohistochemistry, Western blot analysis, RT-PCR and in necrotic area and adjacent non-necrotic area from lesioned femoral neck from ONFH patients and healthy femoral head from femoral neck fracture patients. Receiver operating characteristic (ROC) curve analysis was carried out to confirm the diagnostic value serum CXCL12, PINP and IL-33 with regard to the FICAT grade. Results: Serum CXCL12 levels were significantly lower in non-traumatic ONFH patients compared with healthy controls. CXCL12 mRNA and protein expressions were both significantly decreased in necrotic area in comparison with non-necrotic area and healthy femoral head. Serum CXCL12 concentrations were drastically reduced in patients with FICAT stage 4 compared with stage 3, and CXCL12 concentrations in patients with stage 3 were markedly lower than stage 2. Serum CXCL12 levels were negatively related to FICAT grading. In addition, Serum CXCL12 concentrations were also negatively related to VAS, WOMAC scores and positively correlated with HHS scores. Meanwhile, serum CXCL12 levels were positively correlated with serum PINP and negatively correlated with IL-33 levels. ROC curve analysis implicated that decrease CXCL12 in serum may act as a favorable marker for FICAT grade. Conclusions: Decreased serum CXCL12 concentrations may reflect disease severity of non-traumatic ONFH.
      原文链接:
    • NSTL
    • Elsevier

    Essential trace and toxic elemental concentrations in biological samples of male adult referent and Eunuch subjects

    Afridi, Hassan ImranKazi, Tasneem GulTalpur, Farah NazBaig, Jameel Ahmed...
    8页
    查看更多>>摘要：Background: The influence of essential nutrients such as calcium (Ca), copper (Cu), iron (Fe), magnesium (Mg), selenium (Se), and zinc (Zn) on male fertility has been extensively studied. For reproductive health, adequate spermatogenesis, sperm maturation and motility, as well as optimal sperm function, these essential elements are required. Several pathologic and male infertility disorders have been linked to an imbalance of these elements. Methods: The purpose of this study was to determine the relationship between essential and toxic elements, such as Ca, Cu, Fe, Mg Se, and Zn, as well as cadmium (Cd), lead (Pb), and mercury (Hg) in human biological samples, such as blood, serum, and seminal plasma, from 96 referent male adults aged 21 to 49 years, who were further divided into three age groups. The biological samples of Eunuch people (n = 37), ranging in age from 21 to 42 years, were also analysed for comparison. The method of acid digestion was controlled by a microwave oven. The essential and toxic elements in the oxidised biological samples were determined using atomic absorption spectrometry. Certified reference material of blood and serum was being used to validate the methodology's accuracy. Result: The Ca, Cu, Fe, Mg, Se, and Zn concentrations in blood and serum samples of Eunuch people were found to be lower, whereas Cd, Pb, and Hg levels were significantly higher than those found in data from normal adult male subjects (p 0.001). The results reveal that the concentrations of Ca, Cu, Fe, Mg, Se, and Zn in the seminal plasma of adult male participants in the younger age group (21-29) were higher than those in the older age groups (30-39) and (40-49). Although essential trace element levels in Eunuch people's biological samples were significantly lower than those observed in adult male subjects. Conclusion: Essential elemental deficiency and elevated levels of toxic heavy metals in all biological fluid samples may have significant negative effects on human reproductive health (sperm quality and function), leading to male infertility.
      原文链接:
    • NSTL
    • Elsevier

    Two rapid SARS-CoV-2 disposable devices for semi-quantitative S-RBD antibody levels determination compared with CLIA and ELISA assays at different protective thresholds

    Padoan, AndreaCosma, ChiaraGalla, LuisaBasso, Daniela...
    5页
    查看更多>>摘要：Background and aims: Performance of two disposable devices for identifying subjects with low anti-SARS-CoV-2 protection was compared with that of automated enzyme-linked immunosorbent (ELISA) and chemiluminescent (CLIA) assay. Materials and Methods: In July 2021, 123 healthcare workers (HCW), twice vaccinated by BNT162b2/Comirnaty mRNA (BioNTech-Pfizer), underwent Ab iRapid COVID-19 Quant "Neutralizing" Self-test (iRapid Self-test) and "Neutralizing" Professional-use (iRapid pro) (DIESSE, Diagnostica Senese, Siena, Italy). Simultaneously, serum Ab were determined by Maglumi 2000 plus (anti S-RBD CLIA assay, Snibe Diagnostics, Shenzhen, China) and SARS-CoV-2 "Neutralizing" Ab Chorus ELISA (DIESSE, Siena, Italy). Results were evaluated against two "protective-thresholds", 90 kBAU/L and 506 kBAU/L. Results: HCW mean age, 46.2 (+/- 12.6) years; 26 (20.5%), males, 101 (79.5%), females. The mean time interval (and standard deviation) between the first vaccine dose and Ab determination was 129.5 (+/- 36.4) days and was neither gender (p = 0.879) nor age (p = 0.341) related. With Maglumi, 114 (89.7%) and 43 (33.8%) HCW presented Ab >= 90 kBAU/L and Ab >= 506 kBAU/L, respectively; with Chorus, 96 (75.6%) presented Ab values =506 kBAU/L. CLIA and ELISA agreement was 56.7%. At 90 kBAU/L, iRapid self-test and Pro sensitivities were 98.2% (95% CI: 92.7-99.8), specificity 69.2% (95% CI: 38.6-90.9%) and 76.9% (46.2-95%), respectively. At 506 kBAU/L, iRapid sensitivities were 58.1-91.6%, and specificities, 89-96.6%. On evaluating Ab at <4 and >= 4 months, protective titers had decreased. Conclusions: iRapid semi-quantitative devices had very good overall agreements of 95.1% and 95.9% for detecting individuals with low anti-SARS-CoV-2 protection.
      原文链接:
    • NSTL
    • Elsevier

    Multisystem inflammatory syndrome in children: Inputs of BNP, NT-proBNP and Galectin-3

    Raynor, AlexandreVallee, ClarisseBelkarfa, Anne-LaureLunte, Klara...
    5页
    查看更多>>摘要：Background: Since the COVID-19 pandemic began, a cohort of Multisystem inflammatory syndrome in children (MIS-C) patients has been described. Cardiac involvement is found in 80-85% patients, typically with cardiac dysfunction with or without cardiogenic shock. Here, three cardiac biomarkers, BNP, NT-proBNP and Galectin-3 were compared for the first time in MIS-C in a unique cohort of hospitalized French children. Methods: Fourteen children with MIS-C hospitalized at Necker-Enfants Malades for cardiac management during the first three COVID-19 waves (March 2020-March 2021) were included. All had positive SARS-CoV-2 serology and proven cardiac involvement assessed by transthoracic echocardiography. NT-proBNP, BNP and Galectin-3 were measured at admission, discharge and first follow-up clinic. Results: All admission Galectin-3 measurements were comprised within the reference interval, both in patients with and without cardiogenic shock, and did not vary between admission, discharge and first follow-up clinic. Both median admission BNP and NT-proBNP were higher in children with cardiogenic shock than without. Median admission NT-proBNP was higher than its predictive positive value in heart failure in both groups of children, while median BNP was below its negative predictive value in children without cardiogenic shock but with cardiac dysfunction. Conclusions: Galectin-3 does not seem affected by MIS-C. NT-proBNP seems to increase more precociously than BNP possibly making it a more sensitive marker for screening of heart failure in MIS-C.
      原文链接:
    • NSTL
    • Elsevier