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Journal of Photochemistry and Photobiology
Elsevier Sequoia
Journal of Photochemistry and Photobiology

Elsevier Sequoia

1011-1344

Journal of Photochemistry and Photobiology/Journal Journal of Photochemistry and Photobiology
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    Collective bacterial disinfection by opto-chemical treatment on mature biofilm in clinical endoscope

    Tran, Van NamPark, SuhyunKhan, FazlurrahmanTruong, Van Gia...
    11页
    查看更多>>摘要：The present study proposes an innovative opto-chemical treatment using a basket-integrated optical device (BIOD) to disinfect mature bacterial biofilm on endoscope channels. A BIOD was designed to position an optical diffuser on the central axis of an endoscope channel and to distribute laser light concentrically to the bacterial biofilm on the channel surface. To apply thermal damage and oxidative stress to the bacterial biofilm, a low concentration of a crosslinking agent (glutaraldehyde -0.5%) was combined with 808 nm infrared (IR) and 405 nm blue (BL) laser lights. The applied irradiances of IR and BL were 10 W/cm(2) and 1.6 W/cm(2) for Teflon channel model and 20 W/cm(2) and 3.2 W/cm(2) for a clinical model, respectively. Individual irradiation of either IR or BL for 180 s induced the maximum temperatures of 62 +/- 2 degrees C and 53 +/- 3 degrees C on the biofilm, respectively. The simultaneous opto-chemical treatment reduced a significant population of the bacterial biofilms (7.5-log(10) for Staphylococcus aureus and 7.1-log(10) for Pseudomonas aeruginosa), which were 2.9-fold and 3.9-fold higher than that of the standard treatment with 2% glutaraldehyde (GA) solution, respectively. The proposed opto-chemical disinfection method can help reduce multi-drug resistant bacteria and prevent cross-infection during the clinical usage of a flexible endoscope.
      原文链接:
    • NSTL
    • Elsevier

    Effect of ionizing radiation on human myeloperoxidase: Reaction with hydrated electrons

    Ramos, Daniel R.Furtmueller, Paul G.Obinger, ChristianCanle, Moises...
    7页
    查看更多>>摘要：Myeloperoxidase (MPO) is a myeloid-lineage restricted enzyme largely expressed in the azurophilic granules of neutrophils. It catalyses the formation of reactive oxygen species, mainly hypochlorous acid, contributing to anti-pathogenic defense. Disorders in the production or regulation of MPO may lead to a variety of health conditions, mainly of inflammatory origin, including autoimmune inflammation.We have studied the effect of ionizing radiation on the activity of MPO, as measured by the capacity retained by the enzyme to produce hypochlorous acid as reactive oxygen species after exposure to successive doses of solvated electrons, the strongest possible one-e(-) reducing agent in water. Chlorination activity was still present after a very high irradiation dose, indicating that radiation damage does not take place at the active site, hindered in the core of MPO structure. Decay kinetics show a dependence on the wavelength, supporting that the process must occur at peripheral functional groups situated on external and readily accessible locations of the enzyme.These results are relevant to understand the mechanism of resistance of our innate anti-pathogenic defense system and also to get insight into potential strategies to regulate MPO levels as a therapeutic target in autoimmune diseases.
      原文链接:
    • NSTL
    • Elsevier

    Ultraviolet-A light increases mitochondrial anti-viral signaling protein in confluent human tracheal cells via cell-cell signaling

    Leite, GabrielaRezaie, AliMathur, RuchiBarlow, Gillian M....
    7页
    查看更多>>摘要：Mitochondrial antiviral signaling (MAVS) protein mediates innate antiviral responses, including responses to certain coronaviruses such as severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). We have previously shown that ultraviolet-A (UVA) therapy can prevent virus-induced cell death in human ciliated tracheal epithelial cells (HTEpC) infected with coronavirus-229E (CoV-229E), and results in increased intracellular levels of MAVS. In this study, we explored the mechanisms by which UVA light can activate MAVS, and whether local UVA light application can activate MAVS at locations distant from the light source (e.g. via cell-to-cell communication). MAVS levels were compared in HTEpC exposed to 2 mW/cm2 narrow band (NB)-UVA for 20 min and in unexposed controls at 30-40% and at 100% confluency, and in unexposed HTEpC treated with supernatants or lysates from UVA-exposed cells or from unexposed controls. MAVS was also assessed in different sections of confluent monolayer plates where only one section was exposed to NB-UVA. Our results showed that UVA increases the expression of MAVS protein. Further, cells in a confluent monolayer exposed to UVA conferred an elevation in MAVS in cells adjacent to the exposed section, and also in cells in the most distant sections which were not exposed to UVA. In this study, human ciliated tracheal epithelial cells exposed to UVA demonstrate increased MAVS protein, and also appear to transmit this influence to confluent cells not exposed to UVA, likely via cell-cell signaling.
      原文链接:
    • NSTL
    • Elsevier

    Effects of free radicals from doxycycline hyclate and minocycline hydrochloride under blue light irradiation on the deactivation of Staphylococcus aureus, including a methicillin-resistant strain

    Yuann, Jeu-Ming P.Lee, Shwu-YuanHe, SinWong, Tak-Wah...
    12页
    查看更多>>摘要：Doxycycline hyclate (DCH) and minocycline hydrochloride (MH) are tetracycline antibiotics and broad-spectrum antimicrobial agents. The changes in DCH and MH under blue light (lambda = 462 nm) irradiation in alkaline conditions (BLIA) were investigated. Deactivation caused by superoxide anion radical (O-2(center dot -)) and deactivation from DCH and MH during photolysis on Staphylococcus aureus (S. aureus), including methicillin-resistant S. aureus (MRSA), were studied. DCH is relatively unstable compared to MH under BLIA. The level of O-2(center dot -)-generated from the MH-treated photoreaction is lower than that from DCH photolysis, and the DCH-treated photoreaction is more efficient at inactivating S. aureus and MRSA at the same radiant intensity. DCH subjected to BLIA decreased the viability of S. aureus and MRSA by 3.84 and 5.15 log, respectively. Two photolytic products of DCH (PPDs) were generated under BLIA. The mass spectra of the PPDs featured molecular ions at m/z 460.8 and 458.8. The molecular formulas of the PPDs were C21H22N2O10 and C22H24N2O9, and their exact masses were 462.44 and 460.44 g/mol, respectively. These results bolster the photolytic oxidation that leads to DCH-enhanced deactivation of S. aureus and MRSA. Photochemical treatment of DCH could be applied as a supplement in hygienic processes.
      原文链接:
    • NSTL
    • Elsevier

    Eriodictyol protects skin cells from UVA irradiation-induced photodamage by inhibition of the MAPK signaling pathway

    Pourzand, ChararehYang, JingZhong, Julia LiNisar, Muhammad Farrukh...
    11页
    查看更多>>摘要：Solar UVA irradiation-generated reactive oxygen species (ROS) induces the expression of matrix metalloproteinase 1 (MMP-1), leading to photoaging, however the molecular mechanism remains unclear. In the present study, we found that eriodictyol remarkably reduces UVA-mediated ROS generation and protects the skin cells from oxidative damage and the ensuing cell death. Moreover eriodictyol pretreatment significantly downregulates the UVA-induced MMP-1 expression, and lowers the inflammatory responses within the skin cells. Pretreatment with eriodictyol upregulates the expression of tissue inhibitory metalloproteinase 1 (TIMP-1) and collagen-I (COL-1) at the transcriptional level in a dose-dependent manner. UVA-induced phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 leading to increased MMP1 expression are significantly reduced in eriodictyol-treated skin cells. In addition, eriodictyol pretreatment significantly suppresses inflammatory cytokines and inhibits the activation of MAPK signaling cascades in skin cells. Taken together, our results demonstrate that eriodictyol has both potent anti-inflammatory and antiphotoaging effects.
      原文链接:
    • NSTL
    • Elsevier

    Fabrication of Cu2MoS4 decorated WO3 nano heterojunction embedded on chitosan: Robust photocatalytic efficiency, antibacterial performance, and bacteria detection by peroxidase activity

    Lin, HaitaoLi, TaoJanani, Baadal JushiFakhri, Ali...
    7页
    查看更多>>摘要：In this study, the Cu2MoS4/WO3 supported on chitosan was prepared by precipitation method, and applied to photocatalyst, antibacterial agent and biosensor. The presence of WO3 and Cu2MoS4 crystals were confirmed by XRD analysis. The elemental information was investigated by EDS. FTIR spectra shows the presence of chitosan in nanocomposites. The as-synthesized Cu2MoS4/WO3/Chitosan nanocomposites has a bandgap of 2.18 eV and it is effective for visible light condition. The average particle size of the Cu2MoS4/WO3/Chitosan is 71 nm. The photocatalysis activity Cu2MoS4/WO3/Chitosan was higher than Cu2MoS4 or WO3.The Cu2MoS4/WO3/Chitosan nanocomposites shows the highest efficiency (100%) in photocatalysis degradation of dye under visible light irradiation in 80 min. The center dot O2- plays a main role in degradation process. The as-synthesized Cu2MoS4/WO3/Chitosan nanocomposites depicted the antibacterial activity toward G+/-bacteria. Determination of enterococcus faecalis is important for human health. The DNA template was used to the Cu2MoS4/WO3/Chitosan nanocomposites and applied in detection of enterococcus faecalis by H2O2 and 3,3 ',5,5 '-tetramethylbenzidine in peroxidase like activity. The detection limit of enterococcus faecalis by DNA-Cu2MoS4/WO3/Chitosan in peroxidase-like catalysis was about 55 CFU/mL. Therefore, the Cu2MoS4/WO3/Chitosan can be applied in the photocatalysis, bactericidal and peroxidase process.
      原文链接:
    • NSTL
    • Elsevier

    Photobiology of the keystone genus Metarhizium

    Hallsworth, John E.Corrochano, Luis M.Braga, Gilberto U. L.Brancini, Guilherme T. P....
    15页
    查看更多>>摘要：Metarhizium fungi are soil-inhabiting ascomycetes which are saprotrophs, symbionts of plants, pathogens of insects, and participate in other trophic/ecological interactions, thereby performing multiple essential ecosystem services. Metarhizium species are used to control insect pests of crop plants and insects that act as vectors of human and animal diseases. To fulfil their functions in the environment and as biocontrol agents, these fungi must endure cellular stresses imposed by the environment, one of the most potent of which is solar ultraviolet (UV) radiation. Here, we examine the cellular stress biology of Metarhizium species in context of their photo biology, showing how photobiology facilitates key aspects of their ecology as keystone microbes and as mycoinsectides. The biophysical basis of UV-induced damage to Metarhizium, and mechanistic basis of molecular and cellular responses to effect damage repair, are discussed and interpreted in relation to the solar radiation received on Earth. We analyse the interplay between UV and visible light and how the latter increases cellular tolerance to the former via expression of a photolyase gene. By integrating current knowledge, we propose the mechanism through which Metarhizium species use the visible fraction of (low-UV) early-morning light to mitigate potentially lethal damage from intense UV radiation later in the day. We also show how this mechanism could increase Metarhizium environmental persistence and improve its bioinsecticide performance. We discuss the finding that visible light modulates stress biology in the context of further work needed on Metarhizium ecology in natural and agricultural ecosystems, and as keystone microbes that provide essential services within Earth's biosphere.
      原文链接:
    • NSTL
    • Elsevier

    Nitrogen-limitation exacerbates the impact of ultraviolet radiation on the coccolithophore Gephyrocapsa oceanica

    Jiang, XiaowenZhang, YongHutchins, David A.Gao, Kunshan...
    9页
    查看更多>>摘要：To investigate effects of UV radiation (UVR, 280-400 nm) on coccolithophorids under nutrient-limited conditions, we grew Gephyrocapsa oceanica to determine its resilience to consecutive daily short-term exposures to +UVR (irradiances > 295 nm) under a range of nitrate availabilities (100, 24, 12, 6 and 3 mu M). +UVR alone significantly hampered the growth of G. oceanica, with the synergistic negative effects of +UVR and N-limitation being about 58% and 22% greater than under UVR or N-limitation alone, respectively. Most 3 mu M nitrate cultures died, but those exposed to UVR succumbed sooner. This was due to a failure of photoprotection and repair mechanisms under low N-availability with exposures to UVR. Additionally, the UVR-induced inhibition of the effective quantum yield of photosystem II (PSII) was significantly higher and was further aggravated by N limitation. The algal cells increased photoprotective pigments and UV-absorbing compounds as a priority rather than using calcification for defense against UVR, indicating a trade-off in energy and resource allocation. Our results indicate the negative effects of UVR on coccolithophorid growth and photosynthesis, and highlight the important role of N availability in defense against UVR as well as high PAR. We predict that enhanced N -limi-tation in future surface oceans due to warming-induced stratification will exacerbate the sensitivity of G. oceanica to UVR, while coccolithophores can be potentially more susceptible to other environmental stresses due to increased levels of nutrient limitation.
      原文链接:
    • NSTL
    • Elsevier

    A highly potential acyclic Schiff base fluorescent turn on sensor for Zn2+ ions and colorimetric chemosensor for Zn2+, Cu2+ and Co2+ ions and its applicability in live cell imaging

    Swamy P, Chinna AyyaJayaraj, AnjithaGayathri, M. S.Sivaraman, Gandhi...
    10页
    查看更多>>摘要：Herein, we report two acyclic Schiff base receptors CS-1 and CS-2 capable of being selective fluorescent turn on for Zn(2+)ions and colorimetric chemosensor for Zn2+, Cu2+, and Co2+ ions by showing a colour change from colourless to yellow in 1:1 ratio of acetonitrile and HEPES buffer (1:1, v/v, pH 7.4) without the interference from other metal ions screened (Cd2+, Hg2+, Sn2+, Ni2+, Cr3+, Mn2+, Pb2+, Ba2+, Al3+, Ca2+, Mg2+, K+ and Na+). The fluorescence turn on enhancement towards Zn2+ ions is ascribed to PET blocking, suppression of -C=N- isomerisation, and the ESIPT process. The selectivity, competitivity and reversibility of the synthesised probes (CS-1 and CS-2) made them promising chemosensors for the detection of Zn2+, Cu2+, and Co2+ ions. The density functional theory (DFT) calculations have theoretically endorsed the colorimetric changes in the examined absorption spectra and binding mode of both CS-1/CS-2 with metals ions. In addition, 1H NMR titrations were also consistent with the recognition mechanism of Zn2+ ions with the CS-1/CS-2. Further, the Jobs plot analysis infers a 1:1 stoichiometric ratio for both evaluating receptors CS-1 and CS-2 with Zn2+, Cu2+ and Co2+ ions and was supported by DFT, NMR (only for Zn2+ ions), UV-Visible, and fluorescence spectroscopic studies. Moreover, the detection limits of CS-1 and CS-2 for Zn2+ ions were determined to be 7.69 and 5.35 nM, respectively, which is less compared to the detection limit of Cu2+, Co2+ ions as well as the limit approved by the United State Environmental Protection Agency (US EPA). The probes CS-1 and CS-2 found to show high fluorescence quantum yields at pH = 7 during the titration with Zn2+ as compared with other pHs (5-6 and 8-11). Gratifyingly, fluorescence microscopy imaging in HeLa cells revealed that the pair of receptors can be employed as an excellent fluorescent probe for the detection of Zn2+ ions in living cells, indicating that this facile chemosensor has a huge potential in cellular imaging.
      原文链接:
    • NSTL
    • Elsevier

    Investigating the cellular responses of osteosarcoma to cisplatin by confocal Raman microspectroscopy

    Wang, HaifengLi, JingQin, JieLi, Jie...
    13页
    查看更多>>摘要：Confocal Raman Microspectroscopy (CRM) was employed to clarify the cellular response of cisplatin in osteosarcoma (OS) cells with different dosages and incubation times. The K7M2 mouse osteosarcoma cells were treated by cisplatin in 0 mu M (UT group), 20 mu M (20 T group), and 40 mu M (40 T group) doses for 24-h (24H group) and 48-h (48H group), respectively. Raman spectroscopy was utilized to analyze the drug induced variations of intracellular biochemical components in osteosarcoma cells. The spectral results shows that the main changes in its biochemical composition come from nucleic acids. By adopting three different kernel functions (linear, polynomial, and Gaussian radial basis function (RBF)), principal component analysis combined with support vector machine models (PCA-SVM) was built to address the spectral variations among all investigated groups. Meanwhile, multivariate curve resolution alternating least squares (MCR-ALS) was further utilized to discuss on the chemical interpretation on the acquired spectral results. Moreover, Raman spectral images, which is reconstructed by K-means cluster analysis (KCA) with point-scanned hyperspectral dataset, was applied to illustrate the drug induced compositional and morphological variations in each subcellular region. The achieved results not only prove the application potential of Raman based analytical technique in non-labeled intracellular studies, but also illustrate the detailed compositional and structural information of cisplatin induced OS cell responses from the perspective of multivariate analysis and imaging of Raman spectroscopy.
      原文链接:
    • NSTL
    • Elsevier