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Plant Physiology and Biochemistry
Gauthier-Villars
Plant Physiology and Biochemistry

Gauthier-Villars

0981-9428

Plant Physiology and Biochemistry/Journal Plant Physiology and BiochemistrySCIISTP
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    NtAGO1 positively regulates the generation and viral resistance of dark green islands in Nicotiana tabacum

    Han, HongyanWang, YunruZheng, TianruiPeng, Qiding...
    10页
    查看更多>>摘要：Dark green islands (DGIs) are the outcome of post-transcriptional gene silencing (PTGS) in antiviral immunity, but their characteristics related to PTGS remain largely unknown. In this study, the cucumber mosaic virus (CMV) was inoculated on Nicotiana tabacum plants to explore the PTGS features of DGIs. Our results showed that higher expressions of PTGS-associated genes, especially NtAGO1, present in DGIs. To investigate the role of NtAGO1 in the generation and the antiviral effect of DGIs, NtAGO1 was then over-expressed or knocked out in N. tabacum plants through agrobacterium-mediated genetic transformation. The results showed that more DGIs with larger areas appeared on NtAGO1 over-expressed plants, accompanied by less virus accumulation, less reactive oxygen species production, and seldom membrane damage, whereas fewer DGIs appeared on NtAGO1 knockout plants with more damage on infected plants. In addition, the NtAGO1-participated antiviral process could promote the transduction of the salicylic acid-mediated defense pathway. Taken together, our results indicate that DGIs are maintained by a stronger PTGS mechanism, and NtAGO1 positively regulates the gener-ation and viral resistance of DGIs in N. tabacum.
      原文链接:
    • NSTL
    • Elsevier

    Exogenous gibberellin treatment improves fruit quality in self-pollinated apple

    Liu, ZhiSong, GuozhuLi, WeiLv, Tianxing...
    11页
    查看更多>>摘要：Although a few apple (Malus x xdomestica) varieties are self-compatible, little is known about the differences in fruit quality between self- and cross-pollinated apple. In our current study, we compared the fruit quality of self-pollinated apple plants (cultivar 'Hanfu') in self-pollination or cross-pollinated by another cultivar 'Qinguan'. Analysis of fruit quality revealed substantial differences in the external qualities between self-and cross-pollinated apple fruit, but not in the internal qualities. Fruits harvested from self-pollinated 'Hanfu' were smaller and more asymmetrical than those harvested from the cross-pollinated plants. We developed a mathe-matical model describing how seed number and distribution affect fruit growth. According to this model, the fewer the seeds, the greater the force released from the seeds and the more asymmetrical the fruit. Detection of endogenous hormone and the associated gene expression revealed that gibberellin (GA) levels and GA trans-porter gene expression on the seedless side were significantly lower than those on the seeded side. Analysis of fruit pectin methylesterase activity and demethylated pectin levels indicated that the lack of GA limits fruit cell wall extension. Additionally, spraying the self-pollinating plants with gibberellic acid increased the fruit weight and lowered the proportion of asymmetrical fruit, recovering the exterior fruit quality to that of the cross-pollinated fruit. Furthermore, exogenous GA treatment increased the wax layer thickness and reduced the fruit water loss rate, leading to a dramatic improvement in fruit storage capacity. Therefore, exogenous GA treatment could be used to ensure regular fruit production of self-pollinated 'Hanfu'.
      原文链接:
    • NSTL
    • Elsevier

    Molecular insights into the jasmonate signaling and associated defense responses against wilt caused by Fusarium oxysporum

    Fernandes, Lizelle B.Ghag, Siddhesh B.
    13页
    查看更多>>摘要：Biotic and abiotic stress factors drastically limit plant growth and development as well as alter the physiological, biochemical and cellular processes. This negatively impacts plant productivity, ultimately leading to agricultural and economical loss. Plant defense mechanisms elicited in response to these stressors are crucially regulated by the intricate crosstalk between defense hormones such as jasmonic acid (JA), salicylic acid and ethylene. These hormones orchestrate adaptive responses by modulating the gene regulatory networks leading to sequential changes in the root architecture, cell wall composition, secondary metabolite production and expression of defense-related genes. Fusarium wilt is a widespread vascular disease in plants caused by the soil-borne asco-mycete Fusarium oxysporum and is known to attack several economically important plant cultivars. JA along with its conjugated forms methyl jasmonate and jasmonic acid isoleucine critically tunes plant defense mechanisms by regulating the expression of JA-associated genes imparting resistance phenotype. However, it should be noted that some members of F. oxysporum utilize the JA signaling pathway for disease development leading to sus-ceptibility in plants. Therefore, JA signaling pathway becomes one of the important targets amenable for modulation to develop resistance response against Fusarium wilt in plants. In this review, we have emphasized on the physiological and molecular aspects of JA and its significant role in mounting an early defense response against Fusarium wilt disease. Further, utilization of the inherent JA signaling pathway and/or exogenous application of JA in generating Fusarium wilt resistant plants is discussed.
      原文链接:
    • NSTL
    • Elsevier

    Nitrogen form differently modulates growth, metabolite profile, and antioxidant and nitrogen metabolism activities in roots of Spartina alterniflora in response to increasing salinity

    Hessini, Kamel
    8页
    查看更多>>摘要：Sodium tolerance and nitrogen-source preferences are two of the most fascinating and ecologically important areas in plant physiology. Spartina alterniflora is a highly salt-tolerant species and appears to prefer ammonium (NH4+) over nitrate (NO3-) as an inorganic N source, presenting a suite of aboveground physiological and biochemical mechanisms that allows growth in saline environments. Here, we tested the interactive effects of salinity (0, 200, 500 mM NaCl) and nitrogen source (NO3-, NH4+, NH4NO3) on some physiological and biochemical parameters of S. alterniflora at the root level. After three months of treatments, plants were harvested to determine root growth parameters and total amino acids, proline, total soluble sugars, sucrose, and root enzyme activity. The control (0 mM NaCl) had the highest root growth rate in the medium containing only ammonium and the lowest in the medium containing only nitrate. Except for NO3--fed plants, the 200 mM NaCl treatment generally had less root growth than the control. Under high salinity, NH4+-fed plants had better root growth than NO3?-fed plants. In the absence of salinity, NH4+-fed plants had higher superoxide dismutase, ascorbate peroxidase, glutathione reductase, and guaiacol peroxidase activities than NO3--fed plants. Salinity generally promoted the activity of the principal antioxidant enzymes, more so in NH4+-fed plants. Nitrogen metabolism was characterized by higher constitutive levels of glutamate dehydrogenase (GDH) activity under ammonia nutrition, accompanied by elevated total amino acids levels in roots. The advantage of ammonium nutrition for S. alterniflora under salinity was connected to high amino acid accumulation and antioxidant enzyme activities, together with low H2O2 concentration and increased GDH activity. Ammonium improved root performance of S. alterniflora, especially under saline conditions, and may improve root antioxidant capacity and N-assimilating enzyme activities, and adjust osmotically to salinity by accumulating amino acids.
      原文链接:
    • NSTL
    • Elsevier

    Postharvest melatonin dipping maintains quality of apples by mediating sucrose metabolism

    Fan, YitingLi, CanyingLi, YihanHuang, Rui...
    8页
    查看更多>>摘要：Senescence is a pivotal factor that causes quality breakdown and economic loss of fruit after harvest. In this study, 'Golden Delicious' apples were used as the materials to investigate the effect of melatonin dipping on quality parameters and sucrose metabolism during room temperature storage. Postharvest melatonin treatment inhibited respiratory intensity and ethylene release, increased flesh firmness, soluble sugar, ascorbic acid, and soluble solid contents, and titratable acid in apples. Furthermore, melatonin treatment inhibited acid invertase and neutral invertase activities, increased sucrose synthase and sucrose phosphate synthase activities, and repressed the activities of sorbitol dehydrogenase, sorbitol oxidase and sucrose synthase cleavage in apple fruit. All these findings suggest that exogenous application of melatonin could maintain quality of 'Golden Delicious' apples by mediating the enzyme activity in sucrose metabolism.
      原文链接:
    • NSTL
    • Elsevier

    HrTCP20 dramatically enhance drought tolerance of sea buckthorn (Hippophae rhamnoides L). by mediating the JA signaling pathway

    Yao, YingDong, LijunFu, XiaohongZhao, Lin...
    12页
    查看更多>>摘要：Sea buckthorn, an important ecological and economical tree species, have remarkable drought and salt resistance. The plant-specific transcription factor TCPs play important roles in plant growth, development, and stress responses. However, in sea buckthorn, the molecular mechanism of TCP proteins and their involvement in drought stress are unknown. Here, we found that the expression of HrTCP20 was significantly up-regulated in sea buckthorn under drought stress. Overexpression of HrTCP20 in Arabidopsis thaliana showed that the superoxide dismutase (SOD), polyphenol oxidase (POD), and chlorophyll (SPAD) content was significantly increased by 1.37 and 1.35 times. However, the malondialdehyde (MDA) content decreased by 0.51 times. Our studies further confirmed that silencing HrTCP20 by virus-induced gene silencing (VIGS) led to a decrease in the content of defense enzymes, relative water content (RWC), and an increase of relative electrical conductivity (REC). Silencing HrTCP20 also caused the jasmonic acid (JA) content to decrease in the VIGS-treated tree. Interestingly, we found that JA accumulation content and the expression of HrLOX2, an essential enzyme for JA synthesis, was significantly inhibited in HrTCP20-silenced sea buckthorn under drought stress. Yeast two-hybrid analysis also showed that HrTCP20 is directly bound to HrLOX2. Taken together, the HrTCP20 transcription factor was a positive regulator in drought resistance of sea buckthorn. Further, our findings will provide comprehensive insights into the forest tree defence system of drought stress.
      原文链接:
    • NSTL
    • Elsevier

    Toxicity of BPNSs against Chlorella vulgaris: Oxidative damage, physical damage and self-protection mechanism

    Li, YinghuaLi, ZheMo, FanDeng, Ningcan...
    10页
    查看更多>>摘要：Black phosphorus nanosheets (BPNSs) has extensive application prospect in the fields of optoelectronics and biomedicine, due to its unique physicochemical properties. Therefore, a systematic toxic study is necessary to assess its environmental safety. Herein, BPNSs was prepared by liquid exfoliation procedure, the primary producer Chlorella vulgaris (C. vulgaris) was used as a test subject. After the exposure for 120 h at 15, 45 and 75 mg/ L BPNSs, the cell viabilities were 45.05%, 18.86% and 4.60% for each treatment group, respectively. The extent of lipid peroxidation and peroxidative damage in C. vulgaris was confirmed by measuring reactive oxygen species (ROS) levels, superoxide dismutase (SOD) and catalase (CAT) activities, followed by determination of malondialdehyde (MDA) content. Morphological analysis results (i.e., SEM and TEM) showed that BPNSs adhered to the cell surface and enter the cell to severely damage cell structure. Furthermore, BPNSs were shown to accelerate apoptosis in C. vulgaris by flow cytometry analysis. Finally, GC-MS was used to explore the metabolic regulatory mechanism of C. vulgaris in response to BPNSs stress. The results of this study can provide theoretical support for subsequent studies on the potential enrichment risk of BPNSs in the water environmental food chain.
      原文链接:
    • NSTL
    • Elsevier

    Silicon-induced alterations in the expression of aquaporins and antioxidant system activity in well-watered and drought-stressed oilseed rape

    Saja-Garbarz, DianaLibik-Konieczny, MartaFellner, MartinJurczyk, Barbara...
    14页
    查看更多>>摘要：Progressing climate change necessitates the search for solutions of plant protection against the effects of water deficit. One of these solutions could be silicon supplementation. The aim of the study was to verify the hypothesis that silicon changes aquaporin expression and antioxidant system activity in a direction which may alleviate the effects of drought stress in oilseed rape. The accumulation of BnPIP1, BnPIP2-1-7 and BnTIP1;1 aquaporins and the expression of their genes, the level of catalase, superoxide dismutase activities and hydrogen peroxide content as well as total non-enzymatic antioxidant activity were analyzed in leaf tissue from control and silicontreated oilseed rape plants growing under well-watered and drought conditions. Silicon was applied in two forms - pure silicon and a silicon complex. It was shown that under drought conditions, both pure silicon and the silicon complex (with Fe) significantly increased the accumulation of aquaporins and improved the activity of enzymatic and non-enzymatic components of the antioxidant system, while under well-watered conditions, these effects were observed only in the case of the silicon complex. The presented study proves that silicon supplementation in oilseed rape improves the regulation of water management and contributes to the protection against oxidative stress caused by drought.
      原文链接:
    • NSTL
    • Elsevier