查看更多>>摘要：Pistia stratiotes is a cadmium (Cd) hyperaccumulating plant with strong bioaccumulation and translocation capacity for Cd. A hydroponic experiment was used to evaluate the combined effect of Zinc (Zn) and Cd at different concentrations on leaf growth and metabolism of P. stratiotes. This study revealed the physiological defense and metabolic strategy of responses to Zn-Cd co-pollution. With the Zn50Cd1, Zn50Cd10, Zn100Cd1, and Zn100Cd10 treatments for 9 d, the relative crown diameter, relative leave number, and ramet number of the plant had no significant difference with the control. Under the compound treatments containing Zn50Cd50 and Zn100Cd50, the activity of the glyoxalase system and amino acid metabolism in the leaves were inhibited. The leaf photosynthetic apparatus increased heat dissipation to reduce the damage to the photosystem II (PS II) reaction center caused by excess excitation energy under Zn-Cd stress. This safeguarded the balance between the absorption and utilization of light energy. Compared to the control, the Zn and Cd co-pollution for 9 d had no effect on the reduced glutathione (GSH) and oxidized glutathione (GSSG) contents. There was no effect on the dehydroascorbate reductase (DHAR) and glutathione reductase (GR) activities, but there was increased ascorbate peroxidase (APX) activity and oxidized ascorbic acid (DHA) content. These increased the antioxidant capacity of the ascorbate-glutathione (AsA-GSH) cycle. The treated plants also had increased levels of carnosol and substances related to lipid metabolism including 9, 10-Dihydroxystearate, Prostaglandin G2, Sphingosine, and 13-LHydroperoxylinoleic acid, maintaining the cell stability and resistance to the Zn-Cd stress.
Mendes, Gabriela Galvao MachadoMota, Thatiane RodriguesBossoni, Gabriela Ellen BarretoMarchiosi, Rogerio...
8页
查看更多>>摘要：Lignin is a technological bottleneck to convert polysaccharides into fermentable sugars, and different strategies of genetic-based metabolic engineering have been applied to improve biomass saccharification. Using maize seedlings grown hydroponically for 24 h, we conducted a quick non-transgenic approach with five enzyme inhibitors of the lignin and tricin pathways. Two compounds [3,4-(methylenedioxy)cinnamic acid: MDCA and 2,4pyridinedicarboxylic acid: PDCA] revealed interesting findings on root growth, lignin composition, and saccharification. By inhibiting hydroxycinnamoyl-CoA ligase, a key enzyme of phenylpropanoid pathway, MDCA decreased the lignin content and improved saccharification, but it decreased root growth. By inhibiting flavone synthase, a key enzyme of tricin biosynthesis, PDCA decreased total lignin content and improved saccharification without affecting root growth. PDCA was three-fold more effective than MDCA, suggesting that controlling lignin biosynthesis with enzymatic inhibitors may be an attractive strategy to improve biomass saccharification.
Khan, Muhammad RamzanSchneider, MichaelUzair, MuhammadAqeel, Muhammad...
11页
查看更多>>摘要：Drought is a big challenge for agricultural production. Root attributes are the important target traits for breeding high-yielding sustainable wheat varieties against ever changing climatic conditions. However, the transcriptomic of wheat concerning root architecture remained obscure. Here, we explored RNA-Seq based transcriptome to dissect putative genes involved in root system variations in naturally occurring six genotypes (drought-tolerant and sensitive) of wheat. Global RNA-Seq based root transcriptome analysis revealed single nucleotide poly-morphisms (SNPs) variations and differentially expressed genes. Putative 56 SNPs were identified related to 15 genes involved in root architecture. Enrichment of these genes using GO terms demonstrated that differentially expressed genes (DEGs) are divided into sub-categories implicated in molecular functions, cellular components and biological processes. The KEGG analysis of DEGs in each comparison of genotype include metabolic, biosynthesis of secondary metabolites, microbial metabolism in diverse environments and biosynthesis of anti-biotics. A deeper insight into DEGs unveiled various pathways involved in drought response and positive gravitropism. These genes belong to various transcription factor families such as DOF, C3H, MYB, and NAC involved in root developmental and stress-related pathways. Local White and UZ-11-CWA-8, which are drought-tolerant genotypes, harbor over-representation of most of DEGs or transcription factors. Notably, a microtubule-associated protein MAPRE1 belonging to RP/EB family recruited in positive gravitropism was enriched. Real-time PCR analysis revealed expression of MAPRE1 and PAL genes is consistent with RNA-seq data. The pre-sented data and genetic resources seem valuable for providing genes involved in the root system architecture of drought-tolerant and susceptible genotypes.
查看更多>>摘要：Seed germination is a crucial stage in a plant's life cycle, during which the embryo, surrounded by several tissues, undergoes a transition from the quiescent to a highly active state. Endosperm weakening, a key step in this transition, plays an important role in radicle protrusion. Endosperm weakening is initiated upon water uptake, followed by multiple key molecular events occurring within and outside endosperm cells. Although available transcriptomes have provided information about pivotal genes involved in this process, a complete understanding of the signaling pathways are yet to be elucidated. Much remains to be learnt about the diverse intercellular signals, such as reactive oxygen species-mediated redox signals, phytohormone crosstalk, environmental cue-dependent oxidative phosphorylation, peroxisomal-mediated pectin degradation, and storage protein mobilization during endosperm cell wall loosening. This review discusses the evidences from recent researches into the mechanism of endosperm weakening. Further, given that the endosperm has great potential for manipulation by crop breeding and biotechnology, we offer several novel insights, which will be helpful in this research field and in its application to the improvement of crop production.
查看更多>>摘要：Maize (Zea mays L) is an important cereal with extensive adaptability and multifaceted usages. However, various abiotic and biotic stresses limit the productivity of maize across the globe. Exposure of plant to stresses disturb the balance between reactive oxygen species (ROS) production and scavenging, which subsequently increases cellular damage and death of plants. Tolerant genotypes have evolved higher output of scavenging antioxidative defence compounds (ADCs) during stresses as one of the protective mechanisms. The glutathione peroxidases (GPXs) are the broad class of ADCs family. The plant GPXs catalyse the reduction of hydrogen peroxide (H2O2), lipid hydroperoxides and organic hydroperoxides to the corresponding alcohol, and facilitate the regulation of stress tolerance mechanisms. The present investigation was framed to study the maize GPXs using evolutionary and functional analyses. Seven GPX genes with thirteen splice-variants and sixty-three types of cis-acting elements were identified through whole-genome scanning in maize. Evolutionary analysis of GPXs in monocots and dicots revealed mixed and lineage-specific grouping patterns in phylogeny. The expression of ZmGPX splice variants was studied in drought and waterlogging tolerant (L1621701) and sensitive (PML10) genotypes in root and shoot tissues. Further, the differential expression of splice variants of ZmGPX1, ZmGPX3, ZmGPX6 and ZmGPX7 and regulatory network analysis suggested the splicing and regulatory elements mediated stress responses. The present investigation suggests targeting the splicing machinery of GPXs as an approach to enhance the stress tolerance in maize.& nbsp;
Zahra, NoreenAl Hinai, Marwa SulaimanHafeez, Muhammad BilalRehman, Abdul...
15页
查看更多>>摘要：Photosynthesis is crucial for the survival of all living biota, playing a key role in plant productivity by generating the carbon skeleton that is the primary component of all biomolecules. Salinity stress is a major threat to agricultural productivity and sustainability as it can cause irreversible damage to photosynthetic apparatus at any developmental stage. However, the capacity of plants to become photosynthetically active under adverse saline conditions remains largely untapped. This study addresses this discrepancy by exploring the current knowledge on the impact of salinity on chloroplast operation, metabolism, chloroplast ultrastructure, and leaf anatomy, and highlights the dire consequences for photosynthetic machinery and stomatal conductance. We also discuss enhancing photosynthetic capacity by modifying and redistributing electron transport between photosystems and improving photosystem stability using genetic approaches, beneficial microbial inoculations, and root architecture changes to improve salt stress tolerance under field conditions. Understanding chloroplast operations and molecular engineering of photosynthetic genes under salinity stress will pave the way for developing salt-tolerant germplasm to ensure future sustainability by rehabilitating saline areas.
查看更多>>摘要：Miracle fruit, Synsepalum dulcificum, produces a unique taste-modifying protein, miraculin (MIR), which has an attractive potential for commercial application as a novel low-calorie sweetener. To establish a stable supply system for MIR, a previous study established a platform for recombinant MIR (rMIR) production in tomato plants and demonstrated that native miraculin from miracle fruit (nMIR) and rMIR were almost identical in their protein modifications with N-glycan. However, neither N-glycosylation nor the influence of fruit maturation on the structural changes of N-glycan have been fully characterized in detail. Here, with a focus on N-glycosylation and the contribution of fruit maturation to N-glycan, we reanalyzed the N-glycosylation of the natural maturation stages of nMIR and rMIR, and then compared the N-glycan structures on MIRs prepared from the fruit at two different maturation stages. The detailed peptide mapping and N-glycosylation analysis of MIRs provided evidence that MIRs have variants, which were derived mainly from the differences in the N-glycan structure in nMIR and the N-glycosylation in rMIR and not from the cleavage of the peptide backbone. N-Glycan analysis of MIRs from the maturation stage of fruits demonstrated that N-glycan structures were similar among nMIRs and rMIRs at every maturation stage. These results indicated that the heterogeneously expressed rMIRs had the same characteristics in post-translational modifications, especially N-glycosylation and N-glycan structures, throughout the maturation stages. This study demonstrated the potential of recombinant protein expressed in tomato plants and paves the way for the commercial use of rMIR.
查看更多>>摘要：Saponaria officinalis L. is a perennial plant from the Caryophyllaceae family whose various parts are used in traditional medicine as the treatment agent of skin diseases, blood purifier, diuretic, sudorific, and bile purifier. The cultivation system of hairy roots is a proper alternative for improving the valuable pharmaceutical compounds production compared to other in-vitro methods. The extensive nanotechnology applications in hairy roots cultivation is a sustainable production foundation to produce such active elements. In this study, the effect of various concentrations of titanium dioxide nanoparticles (TiO2 NPs) (0, 10, 20, 30, 50 mg L-1) with two treatments (24 and 48 h) was examined on the growth level, antioxidant capacity, total phenol and flavonoid contents, antioxidant enzyme activities, certain polyphenol compounds and SO6 protein in hairy roots of S. officinalis. According to the results, the maximum (3.09 g) and minimum (0.96 g) fresh weight (FW) of hairy roots were observed in treated culture media with 10 and 20 mg L-1 of TiO2 NPs after 24 and 48 h of exposure times, respectively. The highest rate of total phenol (9.79 mg GLA g(-1) FW) and total flavonoid contents (1.06 mg QE g(-1) FW) were obtained in the treated hairy roots with 50 and 30 mg L-1 of nano elicitor in 24 and 48 h of treatments, respectively. The maximum level of most polyphenols, such as rosmarinic acid, cinnamic acid, and rutin, was produced in 24 h of treatment. The use of TiO2 NP for 48 h with 50 mg L-1 concentration showed the highest production level of SO6 protein.
查看更多>>摘要：Flowering means that the plant enters the reproductive growth stage, which is a crucial stage in the plant life cycle. Delaying flowering time to prolong vegetative growth is an important method to increase biomass yield and saccharification efficiency in switchgrass, It is of great significance to study the molecular mechanism of plant flowering and regulate the process of plant flowering in the process of biomass production. In this study, we identified 55 serine/threonine-protein kinase genes related to flower development from the switchgrass transcriptome database. Simultaneously, we cloned one of them, PvSTK1, whose expression level and differential fold were significantly higher than other members. PvSTK1 is located on chromosome 8N and its protein was in the cell membrane, cytoplasm, and nucleus. The spatio-temporal expression analysis of the PvSTK1 in switchgrass displayed that the PvSTK1 is crucial in vegetative period, however, not in the transition to reproductive period. Overexpression of PvSTK1 in Arabidopsis resulted in down-regulation of flower-promoting genes and up regulation of flower-suppressing genes, thereby delaying flowering. In addition, PvSTK1 caused atrophy of the ovules of the florets at the base of the inflorescence, leading to sterility of the florets. The function of PvSTK1 is to inhibit the development of floral organs, and its overexpression can prolong its vegetative period. In the future, overexpression of the PvSTK1 gene in switchgrass will change the flowering time and increase yield and utilization efficiency of biomass.
查看更多>>摘要：It is well documented that yield superiority of super hybrid rice is linked with its improved photosynthetic capacity and/or efficiency. In natural environments, the amounts of CO2 assimilated by plants was also impacted by the rapidity of leaf photosynthesis response to fluctuations of light. However, it remains unknow whether the high yield of super hybrid rice was associated with photosynthetic traits under dynamic state. Here, photosynthetic traits under steady-and dynamic state in two super hybrid rice varieties (Ylinagyou 3218 and Yliangyou 5867) with high yield and two inbred super rice varieties (Zhendao 11 and Nanjing 9108) with lower yield. Under steady state, the net photosynthetic rate (A*) in super hybrid rice was 25.3% larger compared with inbred super rice. During photosynthetic induction, there was no obvious association of the rapidity of net photosynthesis rate (A) to sunflecks with rice subpopulations. Stomatal conductance (g(s)) of super hybrid rice increased slower than that of inbred super rice. The cumulative CO2 fixation (CCF) during photosynthetic induction was 25.2% larger in super hybrid rice than that in inbred super rice. The primary limitation during induction was biochemical limitation rather than stomatal limitation. There was a significantly positive relationship between A* and CCF, while A* was not related with the induction response rate of A. Overall, A* and CCF in super hybrid rice have been improved together, which contributed to its yield superiority, whereas its yield potential still can be improved by increasing induction rate of A under fluctuations of irradiance.
NSTL
Elsevier