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Plant Physiology and Biochemistry
Gauthier-Villars
Plant Physiology and Biochemistry

Gauthier-Villars

0981-9428

Plant Physiology and Biochemistry/Journal Plant Physiology and BiochemistrySCIISTP
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    Changes in physiological responses and MTP (metal tolerance protein) transcripts in soybean (Glycine max) exposed to differential iron availability

    Haque, A. F. M. MohabubulRahman, Md AtikurDas, UrmiRahman, Md Mostafizur...
    9页
    查看更多>>摘要：Members of MTP (metal tolerance protein) family are potential metal ion transporters, but little is known about how their responses and expression are altered in response to the deficiency and excess of Fe in soybean. In this study, root and shoot length and biomass in addition to leaf chlorophyll score, PSII efficiency and photosynthetic performance index were adversely affected by Fe-deficiency and excess Fe. Fe and S concentrations in the root and shoot, as well as the increased root FCR activity, consistently decreased and increased, respectively, accompanied by elevated Zn levels under Fe deficiency and Fe toxicity. This implies that Fe-uptake of plants subjected to differential Fe availability are likely determined by S and Zn nutritional status. In qPCR analysis, GmMTP5, GmMTP7, GmMTP8, and GmMTP10 genes showed downregulation under Fe shortage, whereas GmMTP6 and GmMTP11 were significantly upregulated due to Fe-toxicity. Further, GmMTP1, GmMTP3, GmMTP6, GmMTP7, and GmMTP10 were significantly induced in response to Fe toxicity, indicating their potential role in metal tolerance. Bioinformatics analysis showed that soybean MTP genes possessed a close relationship with certain Arabidopsis genes (i.e. ZAT, MTPB1) involved in solute transport and metal sequestration. Furthermore, top five motifs of soybean MTP protein correspond to the cation efflux family exhibited strong amino acid and evolutionary similarities with Arabidopsis thaliana. These findings shed light on Fe homeostasis mechanisms in soybean and could be used to regulate Fe uptake through breeding or transgenic manipulations of MTP genes.
      原文链接:
    • NSTL
    • Elsevier

    Understanding plant stress memory response for abiotic stress resilience: Molecular insights and prospects

    Irfan, MohammadSharma, MeghaKumar, PankajVerma, Vipasha...
    15页
    查看更多>>摘要：As sessile species and without the possibility of escape, plants constantly face numerous environmental stresses. To adapt in the external environmental cues, plants adjust themselves against such stresses by regulating their physiological, metabolic and developmental responses to external environmental cues. Certain environmental stresses rarely occur during plant life, while others, such as heat, drought, salinity, and cold are repetitive. Abiotic stresses are among the foremost environmental variables that have hindered agricultural production globally. Through distinct mechanisms, these stresses induce various morphological, biochemical, physiological, and metabolic changes in plants, directly impacting their growth, development, and productivity. Subsequently, plant's physiological, metabolic, and genetic adjustments to the stress occurrence provide necessary competencies to adapt, survive and nurture a condition known as "memory." This review emphasizes the advancements in various epigenetic-related chromatin modifications, DNA methylation, histone modifications, chromatin remodeling, phytohormones, and microRNAs associated with abiotic stress memory. Plants have the ability to respond quickly to stressful situations and can also improve their defense systems by retaining and sustaining stressful memories, allowing for stronger or faster responses to repeated stressful situations. Although there are relatively few examples of such memories, and no clear understanding of their duration, taking into consideration plenty of stresses in nature. Understanding these mechanisms in depth could aid in the development of genetic tools to improve breeding techniques, resulting in higher agricultural yield and quality under changing environmental conditions.
      原文链接:
    • NSTL
    • Elsevier

    Phylogenetic analysis of PP2C proteins and interactive proteins analyze of BjuPP2C52 in Brassica juncea

    Zeng, JingZuo, TonghongLiu, YihuaTao, Hongying...
    7页
    查看更多>>摘要：Brassica juncea var. tumida Tsen et Lee (Tumorous stem mustard) is an unique vegetable in China. Its enlarged tumorous stem was used as main raw material to produce pickle (Zhacai). In practice, early-bolting happens around 15% of planting area all year and inhibits its production. Here, about 209 PP2C proteins were identified through HMMER software and divided into 13 sub-families in B. juncea. BjuPP2C52 belongs to E sub-family, was up-regulated at reproductive growth stages and interacts with BjuFKF1, a key protein in regulating plant photoperiod flowering, in vitro and in vivo. To explore interactive proteins, BjuPP2C52 was used as bait, 12 potential interactive proteins were screened from yeast library, and they are BjuCOL3, BjuCOL5, BjuAP2, BjuAP2-1, BjuSVP-1, BjuFLC-2, BjuSKP1f, BjuA014572, BjuA008686, BjuO002119, BjuB036787 and BjuA019268. Further study verified that 10 out of the 12 screened proteins interacted with BjuPP2C52 in vivo. qRT-PCR was conducted to understand the expression pattern of those 10 interactive proteins in different tissues and development stages in B. juncea. The results showed that BjuCOL3, BjuCOL5, BjuB036787 and BjuA019268 were significantly up-regulated, while BjuA008686 and BjuO002119 were down-regulated in flowers compared with other four tissues. In developmental stages, BjuCOL5, BjuAP2, BjuAP2-1, BjuA014572, BjuB036787 and BjuA019268 were significantly up-regulated, while BjuSVP-1, BjuA008686 and BjuO002119 were down regulated at reproductive stages. Based on the results, BjuCOL5, BjuAP2, BjuAP2-1, BjuSVP-1, BjuA014572, BjuB036787 and BjuA019268 may function in regulating flowering time in B. juncea.
      原文链接:
    • NSTL
    • Elsevier

    Phenotyping to dissect the biostimulant action of a protein hydrolysate in tomato plants under combined abiotic stress

    Cassan, CedricDelmas, FredericFrancesca, SilvanaOttosen, Carl-Otto...
    12页
    查看更多>>摘要：Drought and heat stresses are the main constrains to agricultural crop production worldwide. Precise and effi-cient phenotyping is essential to understand the complexity of plant responses to abiotic stresses and to identify the best management strategies to increase plant tolerance. In the present study, two phenotyping platforms were used to investigate the effects of a protein hydrolysate-based biostimulant on the physiological response of two tomato genotypes ('E42' and 'LA3120') subjected to heat, drought, or combined stress. The free amino acids in the biostimulant, or other molecules, stimulated growth in treated plants subjected to combined stress, probably promoting endogenous phytohormonal biosynthesis. Moreover, biostimulant application increased the net photosynthetic rate and maximal efficiency of PSII photochemistry under drought, possibly related to the presence of glycine betaine and aspartic acid in the protein hydrolysate. Increased antioxidant content and a decreased accumulation of hydrogen peroxide, proline, and soluble sugars in treated plants under drought and combined stress further demonstrated that the biostimulant application mitigated the negative effects of abiotic stresses. Generally, the response to biostimulant in plants had a genotype-dependent effect, with 'E42' showing a stronger response to protein hydrolysate application than 'LA3120'. Altogether, in this study a fine and multi-level phenotyping revealed increased plant performances under water-limited conditions and elevated temper-atures induced by a protein hydrolysate, thus highlighting the great potential biostimulants have in improving plant resilience to abiotic stresses.
      原文链接:
    • NSTL
    • Elsevier

    Investigation of the direct effect of a precision Ascophyllum nodosum biostimulant on nitrogen use efficiency in wheat seedlings

    Langowski, LukaszGoni, OscarIkuyinminu, ElomofeFeeney, Ewan...
    14页
    查看更多>>摘要：Reduction in the greenhouse gas (GHG) emissions and nitrogen (N) pollution of ground water by improving nitrogen use efficiency (NUE) in crops has become an intensively investigated research topic in pursuit of a more sustainable future. Although, distinct solutions have been proposed there are only a few reports documenting the detailed interplay between observed plant growth dynamics and changes in plant N related transcriptional and biochemical changes. It was previously demonstrated that the application of a formulated biostimulant (PSI-362) derived from Ascophyllum nodosum (ANE) improves N uptake in Arabidopsis thaliana and in barley. In this study, the effect of PSI-362 on the growth dynamics of wheat seedlings was evaluated at different biostimulant and N supplementation rates. Wheat grown on N deficient MS medium was also analysed from the first hour of the treatment until the depletion of the nutrients in the medium 9 days later. During this time the biomass increase measured for PSI-362 treated plants versus untreated controls was associated with increased nitrate uptake, with surplus N assimilated by the biomass in the form of glutamate, glutamine, free amino acids, soluble proteins, and chlorophyll. Phenotypical and biochemical analysis were supported by evaluation of differential expression of genetic markers involved in nitrate perception and transport (TaNRT1.1/NPF6.3), nitrate and nitrite reduction (TaNR1 and TaNiR1) and assimilation (TaGDH2, TaGoGAT, TaGS1). Finally, a comparative analysis of the precision biostimulant PSI-362 and two generic ANEs demonstrated that the NUE effect greatly differs depending on the ANE formulation used.
      原文链接:
    • NSTL
    • Elsevier

    Evidence for reduced plasmodesmata callose accumulation in Nicotiana benthamiana leaves with increased symplastic cell-to-cell communication caused by RNA processing defects of chloroplasts

    Robles-Luna, GabrielNedo, AlexanderWang, XuAttorresi, Alejandra...
    7页
    查看更多>>摘要：RNA processing defects in chloroplasts were previously associated with increased plasmodesmata (PD) permeability. However, the underlying mechanisms for such association are still unknown. To provide insight into this, we silenced the expression of chloroplast-located INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) RNA helicase in Nicotiana benthamiana leaves and determined an increase in PD permeability which is caused by a reduction of PD callose deposition. Moreover, the silencing of two other nuclear genes encoding chloroplastic enzymes involved in RNA processing, RH3, and CLPR2, also increased PD permeability accompanied by reduced callose accumulation at PD. In addition, we quantified the plastidic hydrogen peroxide levels using the chloroplasttargeted fluorescent sensor, HyPer, in ISE2, RH3, and CLPR2 silenced N. benthamiana leaves. The levels of chloroplastic hydrogen peroxide were not correlated with the increased cell-to-cell movement of the marker protein GFP2X. We, therefore, propose that defects in chloroplast RNA metabolism mediate PD gating by suppressing PD callose deposition, and hydrogen peroxide levels in the organelles are not directly linked to this process.
      原文链接:
    • NSTL
    • Elsevier

    Melatonin alleviates copper stress to promote rice seed germination and seedling growth via crosstalk among various defensive response pathways

    Li, RuiqingWu, LiquanShao, YafangHu, Qunwen...
    13页
    查看更多>>摘要：Copper (Cu) contamination dramatically affects crop growth and thus threatens crop production; while applications of melatonin (MT) serve as an effective way to tolerate Cu stress for plant development, the underlying mechanism remains largely unknown in rice. Here, we found that Cu toxicity remarkably decreased germination rates and seedling growth compared to the untreated control (CK), while seed priming with a solution of 100 mu M MT significantly alleviated the adverse effects on Cu-stressed seeds. In addition, the MT treatment decreased the accumulation of Cu in seedlings at 7 days after imbibition (DAI), possibly through enhanced Cu sequestration, and improved reserve mobilization through the promoted activity of alpha-amylase and protease in seeds under Cu stress. Interestingly, gibberellin (GA) synthesis was restored to or even exceeded the CK levels in the MT presoaking treatment, while the abscisic acid (ABA) content decreased compared to those of the Cu-stressed seeds, suggesting crosstalk between MT and other phytohormones, e.g., GA and ABA. More importantly, MT pretreatment also significantly promoted the growth of postgermination seedlings. This was largely ascribed to the MT-ameliorated antioxidant system, which consequently reduced the accumulation of Cu stress-induced oxidative products, e.g., hydrogen peroxide (H2O2), malondialdehyde (MDA), and superoxide (O-2.-). Collectively, these results demonstrate that seed priming with MT could greatly mitigate the adverse effects of Cu stress on seed germination and subsequent postgermination growth through crosstalk among various defensive response pathways. This study provides vital guidance for applications of MT in agronomic production.
      原文链接:
    • NSTL
    • Elsevier

    Ultrastructural and hormonal changes related to harmaline-induced treatment in Arabidopsis thaliana (L.) Heynh. root meristem

    Alvarez-Rodriguez, SaraLopez-Gonzalez, DavidAraniti, FabrizioSanchez-Moreiras, Adela M....
    12页
    查看更多>>摘要：Harmaline is an indole alkaloid with demonstrated phytotoxicity and recognized pharmacological applications. However, no information is available concerning its mode of action on plant metabolism. Therefore, the present work evaluated bioherbicide mode of action of harmaline on plant metabolism of Arabidopsis thaliana (L.) Heynh. Harmaline induced a strong inhibitory activity on root growth of treated seedlings, reaching IC50 and IC80 values of 14 and 29 mu M, respectively. Treated roots were shorter and thicker than control and were characterized by a shorter root meristem size and an increase of root hairs production. Harmaline induced ultrastructural changes such as increment of cell wall thickness, higher density and condensation of mitochondria and vacuolization, appearance of cell wall deposits, increment of Golgi secretory activity and higher percentage of aberrant nuclei. The ethylene inhibitor AgNO3 reversed high root hair appearance and increment of root thickness, and pTCSn:: GFP transgenic line showed fluorescence cytokinin signal in stele zone after harmaline treatment that was absent in control, whereas the auxin signal in the transgenic line DR5 was significantly reduced by the treatment. All these results suggest that the mode of action of harmaline could be involving auxin, ethylene and cytokinin synergic/antagonistic action.
      原文链接:
    • NSTL
    • Elsevier

    ATL8, a RING E3 ligase, modulates root growth and phosphate homeostasis in Arabidopsis

    Yugandhar, PoliRaghothama, Kashchandra G.Ramaiah, MadhuvanthiJain, Ajay...
    10页
    查看更多>>摘要：Ubiquitination-mediated post-translational modification of proteins is a pivotal regulatory mechanism involved in the growth and development of the plant. The Arabidopsis T ' oxicos en Levadura (ATL) family is a group of RING-type ubiquitin ligases (E3) and ATL8 is a membrane-localized protein. Here, a reverse genetics approach was used to elucidate the role of ATL8 in phosphate (Pi) homeostasis. Deficiencies of Pi and sucrose (Suc) enhanced the relative expression level of ATL8 in different tissues of the wild-type (Wt). The relative expression level of ATL8 was attenuated and augmented in the mutant (atl8) and overexpression lines (Oe1 and Oe2), respectively. There were significant reductions in different root traits, root hairs, root to shoot ratio, and total Pi content in atl8 compared with the Wt under different Pi regimes. On the contrary, Oe1 and Oe2 lines exhibited enhancement in some of these traits. Noticeably, anthocyanin content was significantly reduced in Oe1 and Oe2 compared with the Wt and atl8 under P-condition. Abscisic acid (ABA) treatment led to an increase in the primary root length of atl8 compared with the Wt, suggesting a cross-talk between ABA and ATL8 on root growth. Furthermore, the relative expression levels of the genes involved in the maintenance of Pi homeostasis (WRKY75, RNS1, E3L, and ACP5) were differentially modulated in atl8, Oe1, and Oe2 compared with the Wt under different Pi regimes. The results revealed the pivotal role of ATL8 in mediating morphophysiological and molecular adaptive responses to Pi deficiency.
      原文链接:
    • NSTL
    • Elsevier

    Color fading in lotus (Nelumbo nucifera) petals is manipulated both by anthocyanin biosynthesis reduction and active degradation

    Liu, JuanWang, YuxinZhang, MinghuaWang, Yunmeng...
    8页
    查看更多>>摘要：Flower color is a key trait that determines the ornamental quality of aquatic lotus (Nelumbo nucifera). Color fading significantly decreases the ornamental value of lotus flowers. However, the molecular mechanism underlying lotus petal discoloration remains largely unknown. Here, the anthocyanin content and global transcriptional profiling of lotus petals of cultivar 'Qiusanse' in four developmental stages were analyzed. Five anthocyanin components were detected, and the total anthocyanin content decreased as the petal color changed from red to nearly white. Moreover, the malondialdehyde (MDA) content and peroxidase (POD) activity increased during color fading. RNA-seq analysis revealed a total of 4,092 differentially expressed genes (DEGs) between petal developmental stages. Notably, oxidoreductase and hydrolase activity related genes were overrepresented in DEGs. The expression pattern of key anthocyanin biosynthesis genes including, CHS, F3H, ANS, UFGT, and transcription factor regulators, including MYBs, WRKYs and bHLHs were correlated with anthocyanin accumulation. Interestingly, DEGs associated with anthocyanin degradation and vacuolar pH regulation, including peroxidase, proton pumps regulators such as WRKY3 and MYB5-like, were significantly upregulated during the late stages of flowering. This study reveals for the first time the transcriptional dynamics during lotus petal discoloration. Our results suggest the involvement of anthocyanin biosynthesis repressors and degrading genes as well as pH regulators in controlling color fading of lotus petals. The study also provides valuable information and candidate genes for improving the lotus flower color.
      原文链接:
    • NSTL
    • Elsevier