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Plant Physiology and Biochemistry
Gauthier-Villars
Plant Physiology and Biochemistry

Gauthier-Villars

0981-9428

Plant Physiology and Biochemistry/Journal Plant Physiology and BiochemistrySCIISTP
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    Controlled atmosphere storage alleviates internal browning in flat peach fruit by regulating energy and sugar metabolisms

    Hu, YangHuan, ChenYe, ZhengwenZhang, Xianan...
    14页
    查看更多>>摘要：Flat peach fruit are cold-sensitive and vulnerable to chilling injury (CI), particularly internal browning (IB) during cold storage, which limits the consumer acceptance and market value of the fruit. Controlled atmosphere (CA) has been used to alleviate IB in fruit. However, the mechanisms of CA on IB in peach remains unknown. This study investigated the effects of CA (3-3.5% Oxygen, 3-3.5% Carbon dioxide, and 93-94% nitrogen) treatment on IB development, sugar metabolism, and energy metabolism in cold-stored (1 +/- 0.5 degrees C) peach. The CA treatment effectively inhibited the development of IB and markedly inhibited the reduction of sugar contents and energy charge. The protein expression of the V-type proton ATPase subunit was significantly inhibited by the CA treatment, accompanied by higher adenosine triphosphate (ATP) content, and energy charge than the control fruit. Notably, the expressions of the pyruvate kinase family of proteins, pyruvate decarboxylases, and sucrose synthase were induced by CA treatment that had complex protein interactions with the ATPase and the energy metabolism pathway. These results indicated that CA treatment enhanced the chilling tolerance attributed to maintaining higher levels of energy status and sugar contents by regulating the expression of key proteins involved in energy metabolism during cold storage and shelf life. Taken together, our study can provide theoretical support for the research and development of fresh-keeping and cold-chain logistics technology.
      原文链接:
    • NSTL
    • Elsevier

    CML8 and GAD4 function in (Z)-3-hexenol-mediated defense by regulating gamma-aminobutyric acid accumulation in Arabidopsis

    Vanegas, DianaMcLamore, Eric S.Jiao, ChunyangGong, Junqing...
    10页
    查看更多>>摘要：(Z)-3-hexenol, a small gaseous molecule, is produced in plants under biotic stress and induces defense responses in neighboring plants. However, little is known about how (Z)-3-hexenol induces plant defense-related signaling. In this study, we uncovered how (Z)-3-hexenol treatment enhances plant resistance to insect attacks by increasing gamma-aminobutyric acid (GABA) contents in Arabidopsis leaves. First, (Z)-3-hexenol increases the intracellular content of calcium as secondary messenger in Arabidopsis leaf mesophyll cells. Both intracellular and extracellular calcium stores regulate changes in calcium content. Then, CML8 and GAD4 transmit calcium signaling to affect (Z)-3-hexenol induced GABA content and plant resistance. Herein, CML8 interaction with GAD4 was examined via yeast two-hybrid assays, firefly luciferase complementation imaging, and GST pull-down assays. These results indicate that (Z)-3-hexenol treatment increased the GABA contents in Arabidopsis leaves based on CML8 and GAD4, thus increasing plant resistance to the insect Plutella xylostella. This study revealed the mechanism of activating plant insect defense induced by (Z)-3-hexenol, which guides the study of volatiles as biological pest control.
      原文链接:
    • NSTL
    • Elsevier

    Systematic functional characterization of cinnamyl alcohol dehydrogenase family members revealed their functional divergence in lignin biosynthesis and stress responses in mulberry

    Kang, XiaoruYidilisi, KeermulaDai, MingjieLiu, Li...
    12页
    查看更多>>摘要：Mulberry (Morus) is used as a feed additive and biofuel materials. Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.95) catalyzes the final step of monolignol biosynthesis and is responsible for various monolignols. Five MaCADs from Morus alba were cloned and functionally characterized in the present study. These MaCADs encoded proteins with 357-364 amino acids, and the putative protein sequences conservatively possessed two Zn2+ binding motifs and an NADP(H) cofactor binding motif. However, MaCAD1, 2, and 5 shared similar amino acids at substrate binding positions that differed from those possessed by bona fide CADs. MaCAD3 and 4 had conservative substrate binding sites, and both phylogenetic and expression profile analysis indicated they were bona fide CADs involved in lignin biosynthesis. The enzymatic assay showed that MaCAD1 and 5 had a high affinity to p-coumaryl aldehyde. MaCAD4 preferentially used coniferyl aldehyde and sinapyl aldehyde as substrates. His-72 and Tyr-124 in MaCAD1 stabilized p-coumaryl aldehyde, and may have resulted in the substrate preference for p-coumaryl aldehyde. Down-regulation of MaCADs in mulberry showed that MaCAD3/4 were dominant CADs that functioned in monolignol biosynthesis, and decreased MaCAD3/4 resulted in significant decreases of lignin content in both stems and leaves. MaCADs exhibited different expression patterns in response to various stresses, indicating their possible diverse roles. MaCAD2 and MaCAD5 may play positive roles in response to drought and cold stresses, respectively. These results provide a systematic functional analysis of MaCADs in mulberry and an important foundation for the genetic modification of the monolignol pathway in mulberry.
      原文链接:
    • NSTL
    • Elsevier

    Simulated microgravity contributed to modification of callogenesis performance and secondary metabolite production in Cannabis Indica

    Darigh, FarzanehIranbakhsh, AlirezaArdebili, Zahra OraghiEbadi, Mostafa...
    12页
    查看更多>>摘要：In vitro plant culture paves the way for meeting the industrial demand of pharmaceutically valuable secondary metabolites. This study intends to monitor how callus cells of Cannabis indica respond to the simulated microgravity (clinorotation; a Man-made technology). Callus initiation resulted from the culture of the leaf explant in a medium supplemented with kinetin (0.5 mgL(-1)) and 2, 4-D (2 mgL(-1)). Calli were treated with microgravity at three exposure times (0, 3, and 5 days). The microgravity treatments increased callus biomass about 2.5-fold. The clinorotation treatments transcriptionally induced the olivetolic acid cyclase (OAC) and olivetol synthase (OLS) genes about 6.2-fold. The tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) genes displayed a similar upward trend in response to microgravity. The applied treatments also stimulated the expression of the ethylene-responsive element-binding proteins (ERF1B) and WRKY1 transcription factors by an average of 7.6-fold. Moreover, the simulated microgravity triggered epigenetic modification in the DNA methylation profile. The HPLC-based assessment validated the high efficacy of the clinorotation treatments to increase the concentration of cannabinoids, including Cannabigerol (CBG) and Cannabidiol (CBD). However, the clinorotated calli contained a lower concentration of Tetrahydrocannabinol (THC) than the control group. The microgravity treatments increased concentrations of proline (79%), soluble sugars (61.3%), and proteins (21.4%) in calli. The biochemical assessment revealed that the clinorotation treatments slightly increased H2O2 concentration. The upregulation in the activities of peroxidase, catalase, and phenylalanine ammonia-lyase enzymes resulted from the microgravity treatments. Both HPLC and molecular assessments validated the significant efficacy of microgravity to enhance the production of cannabinoids.
      原文链接:
    • NSTL
    • Elsevier

    A maize spermine synthase 1 PEST sequence fused to the GUS reporter protein facilitates proteolytic degradation (vol 78, pg 80, 2014)

    Maruri-Lopez, IsraelRodriguez-Kessler, MargaritaRodriguez-Hernandez, Aida AraceliBecerra-Flora, Alicia...
    1页
      原文链接:
    • NSTL
    • Elsevier

    Co-over expression of Ascorbate Glutathione pathway enzymes improve mercury tolerance in tomato

    Bashir, SabeehaJan, NeloferWani, Umer MajeedRaja, Vaseem...
    12页
    查看更多>>摘要：The genetic modification of plants for the removal of inorganic pollutants from contaminated soil and water bodies is an emerging area for addressing environmental concerns. This approach is based on the ability of plants to take up and accumulate heavy metals, with efficiency being dependent on the underlying mechanisms of heavy metal accumulation and tolerance. A robust antioxidant pathway is determinantal for heavy metal uptake and accumulation and, therefore, in this study, we evaluated the transgenic tomato plants installed with Ascorbate Glutathione (ASA-GSH) pathway genes for uptake, accumulation, and response to mercury (Hg). We observed that ASA-GSH overexpressing lines were resilient to Hg stress as they displayed higher photosynthetic activity and increased photosynthetic gas exchange parameters with a concomitant decrease in ion leakage under Hg stress. Additionally, transgenic lines accumulated high osmolytes and showed enhanced activity of antioxidant enzymes. Moreover, the results of SEM and confocal microscopy confirmed least damage to plant tissue in ASA-GSH overexpressing lines compared to wild-type under Hg-stress which was further supported by Atomic absorption study that revealed a significant decline in Hg accumulation in the leaves of transgenic lines compared to wild-type under stress conditions. In conclusion, pyramiding of ASA-GSH pathway genes in tomato plants is an efficient approach for the development of Hg-resistant tomato plants and the reclamation of Hgcontaminated sites.
      原文链接:
    • NSTL
    • Elsevier

    Exogenous spermidine regulates the anaerobic enzyme system through hormone concentrations and related-gene expression in Phyllostachys praecox roots under flooding stress

    Gao, JianshuangQian, ZhuangzhuangZhang, YuheZhuang, Shunyao...
    15页
    查看更多>>摘要：Purpose: Acclimation to hypoxia and anoxia is important in various ecological systems, especially flooded soil. Phyllostachys pracecox is sensitive to flooding, and therefore, it is important to explore ways of alleviating hypoxia stress in the roots. In this study, we investigated the regulatory effect of spermidine (Spd) on flooded P. praecox seedlings. Methods: A batch experiment was carried out in roots treated with Spd under flooding for eight days. The following factors were subsequently measured: growth, survival rate, root respiratory activity, soluble protein and anaerobic respiration enzyme contents (pyruvate decarboxylase, PDC; alcohol dehydrogenase, ADH; lactate dehydrogenase, LDH; alanine aminotransferase, AlaAT), S-adenosylmethionine decarboxylase (SAMDC), nitrate reductase (NR), ACC oxidase (ACO) and ACC synthetase (ACS) activities, free Spd, spermine (Spm) and the diamine precursor putrescine (Put) content, indole-3-acetic acid (IAA) and abscisic acid (ABA) content, ethylene emissions and expression of hormone-related genes. Results: Application of Spd promoted root growth (root length, volume, surface and dry weight) and root respiratory inhibition, improved the soluble protein content, and reduced the O-2(middot-) production rate, H2O2 and MDA content to alleviate the damage of roots under flooding. A significant increase in SAMDC activity, and ABA and IAA contents were also observed, along with a reduction in ethylene emissions, NR, ACO and ACS activities (p < 0.05). Exogenous Spd increased the free Spd and Spm contents in the P. praecox roots, but decreased the free Put content. Taken together, these findings suggest that hypoxia stress was alleviated. Moreover, exogenous Spd up-regulated the expression of auxin-related genes ARF1, AUX1, AUX2, AUX3 and AUX4, and down-regulated the expression of ethylene-related ACO and ACS genes during flooding. In addition, correlation and RDA analysis showed that ARF1, ACO and ACS significantly promoted the expression of auxin, ACO and ACS enzyme activities, respectively (p < 0.05), while ADH, NR, AlaAT, ethylene emissions, Put, Spd, ACS and ACO were significantly correlated with ACS, ACO, and auxin-related gene expression (p < 0.05). Overall, ethylene emissions, ACS and ACO were identified as the main drivers of ethylene and auxin-related gene structure. Conclusions: These results suggest that Spd regulated hormone concentrations, the content of Spd, Spm and Put, and related gene expression, in turn regulating physiological changes such as anaerobic enzyme activity, mitigating flooding stress in the roots and improving overall growth. Spd therefore has the potential to improve the adaptability of P. praecox to flooding stress.
      原文链接:
    • NSTL
    • Elsevier

    Regulation effects of carbonized apple branches on absorption, distribution, and utilization of N-15 single-labeled ammonium nitrate ((NH4NO3)-N-15 or (NH4NO3)-N-15) in Malus hupehensis

    Shi, JunyuanXun, MiSong, JianfeiZhang, Weiwei...
    10页
    查看更多>>摘要：To improve the utilization of nitrogen fertilizer and orchard waste, the apple branches were separated and pyrolyzed into carbonized wood and carbonized bark, and then applied to root-zone soil of potted Malus hupehensis. The physiological characteristics of leaves and roots were detected, and the absorption, utilization, and distribution of (NH4NO3)-N-15 and (NH4NO3)-N-15 in plants were analyzed using the N-15 isotope tracer technique. The results indicated that the net photosynthetic rate and water use efficiency of leaves, the root growth, and the activity of nitrate reductase and glutamine synthetase were greatest increased by 1.0% (w:w) carbonized bark and carbonized wood, and the effect of carbonized bark was more effective. The carbonized bark more effectively increased nitrogen derived from fertilizer (Ndff) value in all organs, the distribution of N-15 in roots, and utilization of the (NH4NO3)-N-15 and (NH4NO3)-N-15 of Malus hupehensis compared with carbonized wood at the same application ratio, and 1.0% ratio performed better than other ratios in these terms. The Malus hupehensis treated with carbonized bark had the highest utilization ratio of (NH4NO3)-N-15 (10.54%) when the application ratio was 1.0%, and the corresponding parameter of (NH4NO3)-N-15 was 12.98%. The soil immobilization capacity of 15N was improved, and carbonized bark resulted in the greatest decrease in the loss ratios of (NH4NO3)-N-15 and (NH4NO3)-N-15 under 1.0% ratio, which decreased by 27.33% and 30.08%, respectively. For reducing nitrogen loss and improving nitrogen utilization, carbonized bark was more effective than carbonized wood, mainly because bark contained more cellulose and less lignin than wood.
      原文链接:
    • NSTL
    • Elsevier

    Cytosolic glucose-6-phosphate dehydrogenases play a pivotal role in Arabidopsis seed development

    He, WenliangSun, HaoCui, ChaiyanWang, Xiangxiang...
    13页
    查看更多>>摘要：Embryo development is essential for seed yield and post-germination growth. Glucose-6-phosphate dehydroge-nase (G6PD), the rate-limiting enzyme in oxidative pentose phosphate pathway (OPPP), is widely involved in plant development and stress tolerance by providing nicotinamide adenine dinucleotide phosphate (NADPH). In this study, the double mutant (g6pd5/6), overexpression line (G6PD5/6OE) and complementation line (g6pd5/ 6Comp) of cytosolic glucose-6-phosphate dehydrogenases (Cyt-G6PD) were used to investigate Cyt-G6PD roles in embryo development of Arabidopsis. The results showed that the germination rate of g6pd5/6 seeds was delayed in comparison with that of Col-0; moreover, 11.5% of g6pd5/6 seeds did not germinate. The dysfunction of Cyt-G6PD resulted in decreased fresh weight and primary root length of g6pd5/6 seedlings. The height and silique length of g6pd5/6 plants were also decreased. Moreover, the abortion rate of siliques and seeds of g6pd5/6 plants were increased compared with those of Col-0, G6PD5/6OE and g6pd5/6Comp lines. However, the dysfunction of Cyt-G6PD did not affect pollen activity; but in g6pd5/6, the embryo development was partially delayed or inhibited. The contents of fatty acids and storage proteins, two main storage materials in Arabidopsis seeds, were decreased in g6pd5/6 seeds. Exogenous application of fatty acids (C18:2; C18:3) alleviated the delayed germi-nation of g6pd5/6 seeds. RT-qPCR results further demonstrated that the early embryo development genes were down-regulated in g6pd5/6. Taken together, Cyt-G6PD plays a pivotal role in plant seed development by regu-lating the transcriptions of early embryo development genes and the accumulation of storage materials (espe-cially fatty acids).
      原文链接:
    • NSTL
    • Elsevier

    Genome-wide association analysis and transcriptome reveal novel loci and a candidate regulatory gene of fatty acid biosynthesis in sesame (Sesamum indicum L.)

    Zhou, WangyiSong, ShengnanDossou, Senouwa Segla KoffiZhou, Rong...
    12页
    查看更多>>摘要：The regulatory mechanisms of fatty acid (FA) biosynthesis and triacylglycerols (TAGs) assembly remain largely misunderstood in sesame. Gas chromatography was used to analyze the natural variation in FA compositions and oil content (OC) in 400 sesame accessions grown in three different environments. The phenotypic data was associated with the newly released SNP data from whole-genome resequencing, and 43 significant loci for FA and OC were identified. Comparative transcriptomics analysis of high-OC and low-OC materials was performed, and 515 differentially expressed genes (DEGs) were identified across three seed developmental stages. By integrating the genome-wide association study (GWAS) and DEGs analysis, twenty candidate genes were identified, of which SiTPS1 (trehalose-6-phosphate synthase 1) has emerged as a key regulatory gene of FAs and TAGs metabolism in sesame. Overexpression of SiTPS1 in transgenic Arabidopsis influenced FA composition and significantly increased OC. Our study provides resources for the markers-based improvement of OC and quality in sesame and other crops.
      原文链接:
    • NSTL
    • Elsevier