查看更多>>摘要：High-performance determination of pyrophosphate ions (PPi) and pH is an important goal in biological systems. In this work, Cu-doped carbon dots (Cu-CDs) were synthesized rapidly and simply via a one-pot hydrothermal method. The as-obtained Cu-CDs, with an average size of 2.55 nm, exhibit an excitation-independent fluorescence emission and possess desirable functional groups of carboxyl and amine, which can be served as fluorescence nanoprobes for detection of PPi based on surface passivation. Under the optimal condition, the linear range for detection of PPi was 0.05-20 mu M, and the corresponding limit of detection (LOD) was 0.013 mu M, indicative of a promising assay for the PPi. Moreover, the fluorescent intensity of the Cu-CDs is linear against pH value from 6 to 8.7 in buffer solution, suggesting the feasibility as a pH sensor. The synthesized Cu-CDs coated fluorescent paper indeed can monitor pH in urine with satisfaction by naked eyes through ultraviolet irradiation. The successful detection of PPi and the visual detection of pH value suggest a highly promising application of Cu-CDs in the field of biosensing. (C) 2021 Elsevier B.V. All rights reserved.
Kuwik, MartaGorny, AgataPisarski, Wojciech A.Pisarska, Joanna...
7页
查看更多>>摘要：Spectral properties of lead-free glasses doped with Dy3+ ions in function of glass formers and glass mod-ifiers were studied. The glass systems in a function of concentration GeO2 and CaO/SrO/BaO which was partially or totally replaced by CaF2/SrF2/BaF2 were synthesized. The visible luminescence spectra for obtained glasses were registered and value of parameters such as ratio of yellow-to-blue luminescence and the Commission Internationale de I'Eclairage (CIE) chromaticity coordinates (x, y) were analyzed in detail. The Y/B increase from 2.97 to 3.8 in systems with increasing of concentration glass former (GeO2), whereas ratio Y/B slightly decreases when the of content fluoride glass modifiers increases. The obtained results confirm that impact on visible luminescence of lead-free borate glasses doped with Dy3+ ions is greater for glass former than glass modifier. Moreover, studied systems can be used for opto-electronic as yellowish emitters. (C) 2021 Elsevier B.V. All rights reserved.
查看更多>>摘要：High-precision spectral data is a necessary prerequisite for quantitative analysis of complex solution components. In order to improve the accuracy of spectral data, this paper proposes a method of synchronous modulation and demodulation. This article also combines the "M + N" theory, cleverly uses the excitation fluorescence of the components in the serum and its self-absorption phenomenon, collects the fluorescence spectrum of the serum sample, and then uses the partial least squares (PLS) method and the cubic optimization model method to establish a model to analyze the urea concentration of serum. At the same time, in order to verify the effectiveness of synchronous modulation and demodulation method, the unmodulated fluorescence spectrum is used to establish the regression model of urea concentration. Compared with the unmodulated fluorescence spectrum modeling results, the fluorescence spectrum modeling results after modulation and demodulation have been significantly improved. In the modeling results of fluorescence spectrum after synchronous modulation and demodulation, the Rc is 0.916753, the RMSEC is 2.05848 mmol/L, the Rp is 0.79663, and the RMSEP is 3.16812 mmol/L, the Rp - all is 0.88879, and the RMSEP-all is 2.32114 mmol/L. The results show that the method of synchronous modulation and demodulation proposed in this paper not only reduces the influence of dark current, ambient light and background noise on the signal-to-noise ratio of the spectral data, but also effectively avoids the error caused by the non-synchronization of the chopper and the spectrometer. Therefore, the method used in this paper not only improves the signal-to-noise ratio and accuracy of spectral data, but also improves the accuracy of spectral quantitative analysis of complex solutions. (C) 2021 Elsevier B.V. All rights reserved.
查看更多>>摘要：Citrate is a tricarboxylate, plays vital role in prostate cancer (PC) and the level of citrate is an indicator for PC identification. Herein, triaminoguanidine carbon dots (TAG-CDs) prepared by one step hydrothermal method and used as a citrate receptor. Notably the TAG-CDs without alkaline treatment were highly fluorescent at pH 7 with high quantum yield (11.3%). TAG-CDs were characterized through TEM, XRD, FT-IR, UV-vis and spectrofluorimetry. It is noted that the average size was of 2.8 nm, the presence of highly disordered carbon, retain the functionality of TAG. The absorbance maxima obtained at 294 nm and good emitting response observed at 396 nm. The Y-aromaticity of receptor guanidinium moiety acts as Lewis acid and have peculiar interaction with Lewis base citrate via electrostatic interaction and also protons in the TAG participate hydrogen bonds with citrate, which causes quenching of TAG-CDs. From the obtained linear quenching equation the LOD was found to be 4 nM. The probe expressed high selectivity, high interference tolerance (500 - fold), fast response in 15 mins and good biocompatible. Finally, TAG-CDs utilized for the intracellular imaging of citrate in live MCF-7 cells, it showed good cytotoxicity and delivered contrast images in presence, absence of citrate. TAG-CDs detected the citrate level in human urine samples, the obtained results are validated with HPLC method. (C) 2021 Elsevier B.V. All rights reserved.
查看更多>>摘要：With the widespread application of pesticide in agriculture, pesticide residues in food have posed serious health risks to human. There is an urgent requirement to detect pesticide residues in food. In this work, a sensitive and effective method was employed to measure thiram residues in fruit using surface-enhanced Raman scattering (SERS) technique. Silver ink was written on photographic paper (AgNPs-photographic paper) directly by robotic writing technique. The AgNPs-photographic paper substrates possessed good SERS activities and high stability among four months. A good linear response between the peaks intensities and the logarithmic concentrations of thiram was obtained with the limit of detection (LOD) of 0.024 ppb. The substrates also exhibited excellent reproducibility with relative standard deviation (RSD) value less than 10% from ten different substrates. SERS mapping was tested to characterize the uniformity of AgNPs-photographic paper, and the RSD value was calculated to be 14.34% at 1377 cm (1) measured by 120 points. The LOD values of apple and peach juice adulterated with thiram were 0.0024 and 0.024 ppm, respectively. The LOD values of thiram residues on apple and peach peels were both 0.25 ng/ cm(2). It was demonstrated that the substrates prepared by robotic writing technique had great potential for practical application in food safety inspection. (C) 2021 Elsevier B.V. All rights reserved.
de Carvalho, Fernando Rodriguesda Silva, Fernandode Lima, RicardoBellotto, Andrezza Correia...
13页
查看更多>>摘要：The charge-transfer complexes (CTC) between electron donor iodide and pyridinium dimers and monomers as acceptors have been studied spectrophotometrically in acetonitrile, DMSO and water. The dimers were: N,N'-alkyldiyl-bis(4-cyanopyridinium) and N,N'-alkyldiyl-bis(2-bromopyridinium), and the monomers were: N-alkyl-4-cyanopyridinium and N-alkyl-2-bromopyridinium, bridged by n methylene spacers. The formation constant (K-CTC), molar absorptivity (eCTC), fluorescence-quenching constant (K-SV) were determined. The results indicate that the stoichiometry of the CTC for dimers and monomers is 1:1 (equimolar ratio), and its formation is favored for dimers more than for monomers, especially dimers with short methylene spacers forming a "sandwiched type-complex" in which the iodide is close to the two pyridinium rings. Solvents with low polarity favored the complex, which was destroyed by the presence of water. The experimental studies were complemented with theoretical studies with quantum mechanics (QM) calculations using density functional theory (DFT) and molecular mechanics (MM) simulations with Molecular Dynamics for identify the most stable conformers in acetonitrile solution. The electronic excitations were calculated with sequential QM/MM hybrid method, showing a charge transfer wavelength in agreement with the UV-Vis absorption spectra obtained experimentally. It confirms the "sandwiched type-complex" conformers favoring the interaction of the iodide with one pyridinium rings and simultaneously forming one, or two, hydrogen bonds with the alkyl chain and addi-tionally, for the case of dimers, with the other pyridinium ring. (C) 2021 Elsevier B.V. All rights reserved.
查看更多>>摘要：Hydrazine plays an important role in chemistry, pharmaceuticals, agriculture and aerospace. However, it is not to be underestimated and has been identified as harmful to the human body. Therefore, it is significant and urgent to develop the detection of hydrazine in vivo and in vitro. Here, the probe TAN was synthesized by using benzothiazole derivatives as the fluorophore and 2,3-diaminomaleonitrile as the identified group to detect hydrazine. The presence of hydrazine resulted in a colorimetric and ratiometric fluorescence response of the probe based on the formation of hydrazone. The detection limit of TAN was 0.31 mu M for hydrazine. In addition, the probe TAN was successfully used to visualize hydrazine in living HepG-2 cells and mouse with low cytotoxicity and excellent biocompatibility. (C) 2021 Elsevier B.V. All rights reserved.
查看更多>>摘要：It is urgently needed to develop NIR-fluorescent probe for detection of human serum albumin (HSA) since the interference of short-wavelength-fluorescence from endogenous species in real serum and urine. However, most previous reports were located in the short-wavelength region (<600 nm). In this work, a series of dicyanoisophorone (DCO)-based fluorophores 1-4 with different donor groups have been designed and investigated. A systematic study of their photophysical properties has been carried out. Among these probes, 4 exhibited NIR emission with the highest fluorescence brightness and the most sensitive signal response to HSA. Further studies demonstrated that 4 could strongly bind into the DS1 pocket of HSA with a 1:1 ratio. Importantly, the method based on 4 has been proven to be capable of sensing HSA in real serum and urine samples. (C) 2021 Published by Elsevier B.V.
查看更多>>摘要：Selective and straightforward kinetic spectrophotometric methods were developed to quantify nateglinide (NTG) in pharmaceutical dosage forms. Fixed time (Delta A) and the equilibrium methods utilized the reaction of NTG with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethyl sulfoxide (DMSO) with heating at 80 degrees C for 25 min to form a stable yellow-coloured Meisenheimer complex, which absorbs maximally at 421 nm. The optimization was achieved by utilizing the Box-Behnken experimental design (BBD) combined with response surface methodology (RSM). In which three significant factors were studied, namely, CDNB volume (A), heating temperature (B) and heating time (C) against the absorbance as a response. Method validation presented the International Conference on Harmonisation (ICH) parameters such as specificity, selectivity, linearity, precision, accuracy, limit of detection (LOD), limit of quantitation (LOQ), robustness and solution stability. The LOD and LOQ values were 0.48, 1.46, and 0.21, 0.62 mu g/ml, respectively, for a fixed time (Delta A) and equilibrium methods with the linear dynamic range of 115 mu g/ml. Furthermore, Youden's robustness test using factorial combinations of the selected analytical parameters was performed and investigated its influence with alternative conditions. All results were reproducible and quickly adopted for routine analysis of NTG in pharmaceutical formulations and laboratory preparations. (C) 2021 Elsevier B.V. All rights reserved.
查看更多>>摘要：Aflatoxin is a highly toxic substance dispersed in peanuts, which seriously harms the health of humans and animals. In this paper, we propose a new method for aflatoxin B1(AFB1) detection inspired by quantitative remote sensing. Firstly, we obtained the relative content of AFB1 at the sub-pixel level by subpixel decomposition (endmember extraction, non-negative matrix decomposition). Then we modified the transfer learning models (LeNet5, AlexNet, VGG16, and ResNet18) to construct a deep learning regression network for quantitative detection of AFB1. There are 67,178 pixels used for training and 67,164 pixels used for testing. After subpixel decomposition, each aflatoxin pixel was determined to contain content, and each pixel had 400 hyperspectral values (415-799 nm). The experimental results showed that, among the four models, the modified ResNet18 model achieved the best effect, with R-2 of 0.8898, RMSE of 0.0138, and RPD of 2.8851. Here, we implemented a sub-pixel model for quantitative AFB1 detection and proposed a regression method based on deep learning. Meanwhile, the modified convolution classification model has high predictive ability and robustness. This method provides a new scheme in designing the sorting machine and has practical value. (C) 2021 Elsevier B.V. All rights reserved.
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