查看更多>>摘要：The small molecule biological thiols, such as Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play crucial roles in maintaining various cellular vital activities. In the organism, abnormal levels of small-molecule biological thiols have been associated with a variety of diseases. Therefore, quantitative determination of biological thiols, especially Cys, is significant for understanding their functions in various biological processes. Thus, in this work we designed a new fluorescent probe Ty-Cys1 with a large Stokes shift of 207 nm to monitor Cysteine. The maximum absorption wavelength of Ty-Cys1 was 418 nm, and the maximum emission wavelength was 625 nm. Significantly, the novel probe Ty-Cys1 was effectively in detecting of Cys changes in living cells, zebrafishes, and living mice. (c) 2022 Elsevier B.V. All rights reserved.
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