Li, MotingLi, XueqiMcClements, David JulianShi, Meirong...
9页
查看更多>>摘要：The molecular characteristics and functional properties of lactoferrin (LF)-hyalumnic acid (HA) non-covalent complexes and conjugates formed by physical mixing and chemical conjugation were compared. LF and HA interactions were characterized using phase behavior, particle size, zeta-potential and isothermal titration calorimetry (ITC) analysis. The physicochemical and functional properties of the LF-HA complexes and conjugates were also measured, including their foaming, emulsifying, and antioxidant capacities. LF and HA formed molecular complexes over a wide range of pH values, resulting in changes in particle size, charge and structure. The net charge and thermal stability of the proteins in the LF-HA complexes and conjugates were higher than that of free proteins. Covalently attaching HA to LF significantly (p < 0.05) improved its emulsifying capacity, with the highest emulsifying activity index (47.7 +/- 0.1 m(2)/g) and emulsion stability index (83.2 +/- 0.4 min) being obtained, but it did not improve its foaming properties. The antioxidant capacity of LF in complexes and conjugates was increased by around 9% and 10%, respectively. This study showed that the functional performance of LF could be enhanced by forming complexes or conjugates with HA, which may extend its use in a variety of food applications.
查看更多>>摘要：Mannitol is a polyol commonly used in food and pharmaceutical industries. Its biological large-scale production is hindered by the elevated costs of fructose-rich feedstocks. Grape must could be an interesting substrate for this process, due to its bulk production and to the commercial imbalance caused by unsold wine stocks. In the present work, red must and white must were assessed as feedstocks for mannitol production employing three Lactobacillales species. It was experimentally determined that nutrient supplementation could be limited to manganese and yeast extract, and that grape must should be diluted with water to improve mannitol production. Under optimal conditions, Lactobacillus intermedius NRRL B-3693 produced 68.9 g/L mannitol from red must and 79.8 g/L mannitol from white must in 48 h, attaining yields of 0.888 and 0.895 mol/mol, respectively. In order to further reduce costs, yeast extract was replaced by wine lees, resulting in mannitol concentrations of 59.4 g/L for red must and 65.6 g/L for white must in 144 h by using red wine lees and L. intermedius NRRL B-3693. Therefore, winery surplus and by-products could be used to produce mannitol, thus opening new biorefinery opportunities.
查看更多>>摘要：Plant-based feedstock nutritionally and functionally rich are evermore requested in the food industry, although sustainability is a must. An untapped and sustainable source is hemp seed bran (HPB), which is a byproduct of industrial hemp seed flour. In this research we have studied the fermentation of HPB with different beneficial bacteria with the intention to valorize HPB for further food applications as a fiber supplement. Prebiotic activity was tested in vitro, and microbiological features were monitored and studied, as fermentation process and release of volatile organic compounds (VOCs). Results indicate that when HPB is fermented there is an in increase in the number of terpenes (7 compounds more at the end of fermentation) and in the abundance of organic acids (26.0-fold more of Butyric acid), particularly when fermentation is conducted by a bacterial pool. Besides, p-Cymene, Myrcene, and Eugenol are the VOCs majorly correlated to prebiotic activity. Although other studies must be conducted, this paper suggests that HPB should be valorized as a substrate to produce sustainable and chemical free prebiotics.
查看更多>>摘要：The aim of this study was to investigate the extraction effects of the simultaneous extraction and separation of pumpkin seed oil (PSO), pumpkin seed protein (PSP) and pumpkin seed polysaccharide (PSPS) using ultrasonicassisted three phase partitioning (UTPP). The effects of (NH4)2SO4 addition, t-butanol to slurry ratio, pH, sonication power, irradiation time and duty cycle on the extraction yields of PSO, PSP and PSPS were investigated, and the fatty acid profiles and basic physicochemical properties of PSO extracted by the UTPP process were examined. Based on the single-factor tests, the Box-Behnken design and response surface methodology were selected for numerical optimization to obtain the best response values. The optimal extraction yields of PSO, PSP and PSPS were 39.79 %, 14.30 % and 1.97 % at an (NH4)2SO4 addition of 30 g/100 mL, a t-butanol to slurry ratio of 1.0:1.0 (mL:mL), pH 5, an ultrasonic power of 118 W, an irradiation time of 20 min, and a duty cycle of 60 %. The fatty acid composition and physicochemical properties of PSO extracted by UTPP were similar to those obtained by other methods. Therefore, UTPP is an efficient technique for the simultaneous extraction and separation of PSO, PSP and PSPS from pumpkin seeds.
查看更多>>摘要：The optimization of microwave-assisted phosphorylation modification by sodium tripolyphosphate (STP) on mung bean protein (MBP) and its effect on functional and structural characterizations was studied. The results illustrated that the optimum phosphorylation conditions were: STP/protein ratio of 0.063 g/g, microwave power of 590 W, microwave time of 155 s, and pH of 7.8. As a result, the maximum phosphorylation degree of MBP, 3.27%, was reached at the optimized conditions. Also, structural characterization by X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) revealed that the phosphate groups of STP were bound to the -NH2 and -OH groups of MBP with covalent interactions (C-O-P and C-N-P bounds). The enhanced functional properties of phosphorylated MBP may be due to the introduction of phosphate groups, which may form a large number of hydrogen bonds with water molecules, thus increasing the binding between proteins and water molecules. Furthermore, the introduction of phosphate groups increased the electronegativity of the protein system, improved the electrostatic repulsion between protein molecules, and made them disperse in the solution system more efficiently. This study proposed that microwave-assisted phosphorylation appeared to be a promising technique to expand its application in food systems.
查看更多>>摘要：This study was conducted to evaluate the combined effects of brown algal polysaccharides, low-molecular weight (LMW)-alginate and laminaran, and their responsible gut indigenous bacteria (RIB) on the host. The effects of the alginate- and laminaran-degrading bacterial strains isolated from caecum of mice (Bacteriocides acidifaciens PS4 and Bacteriocides intestinalis ALB-11) and human faeces (Bactroides xylanisolvens H-Alg-1, Bactroides uniformis H-Lam-1, Bactroides finegoldii JCM 13345(T), and Erysipelatoclostridium ramosum JCM 1298(T)) on DPPH radical scavenging capacity and NO secretion from murine macrophage RAW264.7 cells were determined. The DPPH radical scavenging capacities of the culture with polysaccharides, particularly laminaran, was 1.8-12 times higher than that of the culture without polysaccharides. NO secretion was increased by 1.4-3.5 times from RAW264.7 cells by the bacterial cells (final optical density at 600 nm = 1.0) incubated with the polysaccharides, particularly LMW-alginate. The NO secretion induced by Escherichia coli O111 lipopolysaccharide was reduced by 34-57% in the culture supernatant incubated with laminaran. These results suggest that fermentable polysaccharides in brown algae have antioxidant and immunomodulation capacities in the gut environment composed of RIB, which may be useful for preventing lifestyle- and ageing-related diseases in the host.
查看更多>>摘要：Biofilm with compact structure was assembled through layer-by-layer casting technology. Herein, triple-deckerlike films were prepared using alginate (ALG) and TEMPO-oxidized nanocellulose (TOCN), followed by the comparisons of film properties before and after Ca2+ crosslinking. Addition of TOCN as a reinforcement increased the mechanical properties of ALG film significantly, e.g. addition of 7.5 % of TOCN increased the tensile strength (TS) of the film from 92.22 to 112.12 MPa. After Ca2+ treatment, the properties of the crosslinked film were further enhanced where the TS increased to 126.23 MPa compared with the pristine film (99.13 MPa). Besides, Ca2+ treated film revealed a higher thermal stability than the non-crosslinked ones. Scanning electron microscopy and energy dispersive spectrum, and flourier transform infrared spectroscopy showed the uniform distribution of Ca2+, and the enhanced hydrogen and ionic interactions. This phenomenon further revealed that the carboxylic groups on the surface of TOCN might participate in the construction of structure of ALG-based film. The increased insolubility and mechanical strength as well as the reduced water vapor permeability of the crosslinked films could remarkably extend the use of polysaccharide in various applications, especially food packaging.
查看更多>>摘要：The present study aimed to conduct co-pigmentation of anthocyanin in pomegranate juice with the extracted pomegranate peel extract (PPE) by the beta-cyclodextrin (beta-CD) assisted method. The formation of the complex between phenolic compounds and beta-CD was approved by FT-IR, DSC, and SEM analyses. The results showed that fortification with PPE significantly enhanced the stability of total phenolic, total flavonoid content, and antioxidant activity during the storage time. Furthermore, a kinetic study of anthocyanin degradation indicated that fortification of juices with PPE decelerated the degradation rate while enhancing their half-life. At the end of the 60-day storage period, the sample containing 600 mg/L PPE showed a higher a* color index than the control sample. Thus, it can be concluded that the PPE- beta-CD complex could be used as a co-pigmentation agent in anthocyanin-rich juices to stabilize the color and phenolic compounds.
查看更多>>摘要：In this study, bilayer emulsion loading lycopene was prepared using lycopene oil solution (0.8 wt%) as oil phase, whey protein isolate (WPI) solution (0.6 wt%) as the first layer and the chitosan solutions (0-1.50 wt%) as the second layer. The effect of chitosan concentration on droplet size, zeta (zeta)-potential and physical stability of the bilayer emulsion was evaluated and the bilayer emulsion stabilized by chitosan solution of 0.5 wt% concentration showed the best physical stability. In addition, the effects of environmental stress, pH and ionic strength on the bilayer emulsion were measured and the result proved that the bilayer emulsion could provide a better protection for lycopene encapsulated than the WPI monolayer emulsion. Besides, different antioxidants (oil soluble: oil-soluble tea polyphenol, ascorbyl palmitate and vitamin E; hydrosoluble: bamboo leaf flavonoid, D sodium erythorbate and vitamin C) were added into the oil phase, the first layer and the second layer of the lycopene bilayer emulsions, respectively. The physical characteristics, storage stability and light stability of the bilayer emulsion were then evaluated to determine the effects of antioxidants in different locations on the emulsion.
查看更多>>摘要：The present work reports an experimental study on the optimization of phenolic compounds extraction from Citrus x paradisi peels and the development of active packaging. Response surface methodology (RSM) was applied to investigate the effect of microwave-assisted extraction (MAE) for the recovery of total phenolic compounds (TPC). The TPC in the optimized extract were 31.10 mg gallic acid equivalent/100 g of dry weight (mg GAE/100 g dw) with strong antioxidant capacity. The bioactive compounds in the optimized extract were determined by ultrahigh performance liquid chromatography coupled to quadrupole time-of-flight with high energy mass spectrometry (ILPLC-QTOE-MSE). Forty-five compounds were detected and qualified. The optimized extract obtained by MAE was used as an active antioxidant in multilayer food packaging films. The multilayer low-density polyethylene (LDPE)/polyethylene terephthalate (PET) containing 10% of MAE optimized extract provided the most antioxidant power acting as a free radical scavenger.
NSTL
Elsevier