首页期刊导航|Cytologia : International journal of cytology
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Cytologia : International journal of cytology
International Society of Cytology
Cytologia : International journal of cytology

International Society of Cytology

0011-4545

Cytologia : International journal of cytology/Journal Cytologia : International journal of cytology
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    Natural and Artificial Photosymbiosis in Vertebrates

    Okabe, YojiMatsunaga, Sachihiro
    4页
    查看更多>>摘要：Photosymbiosis occurs in symbiotic relationships between heterotrophs and photosymbiotic organ-isms, where a photosynthetic symbiont provides photosynthate to a host. While almost all known host animals are invertebrates, experiments have determined that artificial symbiotic relationships can be established between vertebrates and phototrophs. The ability to generate photosymbiotic relationships in vertebrates has important applications for medical treatments and commercial resource production.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Cell Cycle Delay, Pro-Metaphase Arrest and C-Metaphase Inducing Effects of Petroleum Ether Fraction of Leaf Aqueous Extract of Clerodendrum viscosum Vent.

    Kundu, Lalit MohanRoy, Gobinda ChandraBarman, ManabenduRay, Sanjib...
    7页
    查看更多>>摘要：Clerodendrum viscosum is a traditionally used medicinal plant. The present study aimed to analyze cell cycle delay, pro-metaphase arrest, and c-metaphase inducing effects of the petroleum ether fraction (AQPEF) of leaf aqueous extract of C. viscosum (LAECV). The LAECV was fractionated with petroleum ether and its metaphase arrest, cell cycle delay, and c-metaphase inducing activities were tested on A. cepa root tip cells. The AQPEF induced cell cycle delay, and colchicine like metaphase, c-metaphase, in A. cepa root tip cells. Thus, the present study explores AQPEF as an active fraction of LAECV having metaphase arresting, cell cycle delay, and c-metaphase inducing potentials.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Mitotic Index Reduction and Cytotoxic Effects of Leaf Aqueous Extract of Maesa macrophylla (Wall.) A. DC. in Allium cepa Root Tip Cells

    Barman, AnuvaRay, Sanjib
    5页
    查看更多>>摘要：Maesa macrophylla (Wall.) A. DC. is a traditionally used medicinal plant. The present study aimed to analyze the mitotic index reducing and cytotoxic potentials of the leaf aqueous extract of M. macrophylla (LAEMM) in Allium cepa root tip cells. The 48-h aged A. cepa mots were treated with LAEMM (0-4mg mL(-1)) for 2-72h and the mot growth retardation (RGR), mitotic index (MI) reduction, and mitotic abnormalities (MAs) like sticky and vagrant chromosomes, :anaphase bridge, polar deviation at metaphase or anaphase, etc. were analyzed. The LAEMM induced concentration and time-dependent RGR effects, MI reduction, and the formation of different types of MAs. Thus, the present work explored here cytotoxic effects of LAEMM in A. cepa mot tip cells.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Cytotoxic Effects of Scutellaria discolor Colebr. Aqueous Extract in Allium cepa Root Tip Cells

    Chakraborty, TistaRay, Sanjib
    5页
    查看更多>>摘要：Scutellaria discolor Colebr. is a traditionally used annual herb that occurs in the Himalayan regions of India. The present study aimed to analyze the cytotoxic effects of S. discolor aqueous extract on Allium cepa root tip cells. The equal-sized roots, 48 h aged, were treated with the aqueous extract of S. discolor (AESD) (0.5-4 mg mL(-1)) and colchicine (0.4 mg mL(-1)) for 4 h continuously and then recovered for 16 h (4+16 h R) in distilled water. The AESD induced colchicine-like mitotic abnormalities; sticky chromosome, vagrant and laggard chromosomes, chromosomal fragments, anaphase bridges, stellate anaphase, micronucleus, and polyploidy, indicating it may have like colchicine prospective therapeutic and cytotoxic applications.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Micronuclei and Polyploidy Inducing Effects of Leaf Aqueous Extract of Clerodendrum inerme (L.) Gaertn. in Allium cepa Root Apical Meristem Cells

    Ray, SanjibBarman, Manabendu
    6页
    查看更多>>摘要：Clerodendrum inerme is a widely used medicinal plant in tropical and subtropical regions of the world. The present study aimed to analyze the micronuclei (MN) and polyploid (PP) cell-inducing potentials of the leaf aqueous extract of C. inerme (LAECI) in Allium cepa root tip cells and compared with colchicine induced MN and PP in A. cepa root tip cells. The LAECI induced increased frequency of MN and PP in root tip cells at 4h treatment followed by 32 h water recovery and the effects were found to be comparatively higher than the colchicine effects. Thus, LAECI may contain bioactive phytoconstituents having MN and PP inducing effects on A. cepa root tip cells, suggesting its colchicine-like application in karyotyping and plant breeding programs.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Comparative Analysis of Different Chemical Mutagens in Inducing Chromosomal Aberrations in Meiotic Cells of Triticum aestivum L.

    Alam, QamreKhah, Mushtaq AhmadAzad, Z. R. Azaz Ahmad
    7页
    查看更多>>摘要：Chromosomal aberration assessment is an important index in mutation breeding for determining the mutagen potency, which helps to deduce an optimum level of mutagen dose tolerable by the species. In this study, the genotoxic effects of ethyl methane sulphonate (EMS), methyl methane sulphonate (MMS) sodium azide (SA), and colchicine were studied on the meiotic cell division of Triticum aestivum L. The results demonstrated that these chemical mutagens cause various types of meiotic anomalies, such as univalents, multivalents, chromosome stickiness, unoriented chromosomes, precocious chromosome movements, chromatin bridges, lagging chromosomes, acentric fragments, and micronuclei. A substantial influence of chromosomal aberrations on the post-meiotic product was also experienced. Irregular sporads in the form of triads, dyads, monads, and poly-ads, along with regular tetrads were witnessed. The maximum chromosomal aberrations were observed at higher concentrations of the mutagenic treatments. The highest proportion of pollen mother cells showing meiotic aberrations was induced by EMS followed by colchicine, MMS, and SA. The frequency of fertile pollens was seen to decline from control to the highest concentration of mutagens due to anomalies of preceding stages. In addition, mutagenic treatments decreased gennination and survival percentage as well. The treatment concentrations ranging from 0.2-0.3% were found favorable in creating significant cytogenetic variability and average lethality and thus, could be beneficial in future cytological and mutation breeding programs.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Cytotoxic and Cytostatic Effects of Leaf Aqueous Extract of Crinum asiaticum in Allium cepa Root Apical Meristem Cells

    Das, RiaGoswami, SourenGhosh, PoulomiRoy, Sujit...
    6页
    查看更多>>摘要：Crinum asiaticum is a well-known medicinal plant of the family Amaryllidaceae. The present study aimed to analyze the cytotoxic and cytostatic effects of leaf aqueous extract of C. asiaticum (LAECA). The 48-h aged Allium cepa roots were treated with LAECA (0.25-2.0 mg mL(-1)) and root growth inhibition, mitotic index (MI), chromosomal and cytological aberrations were analyzed. The LAECA induced a concentration-dependent root growth retardation, decreased MI, and increased the various cytological aberrations, like giant cells, hyperchromasia, sticky chromosome, chromosome break, polar deviation, and binucleated cells in A. cepa root apical meristem. Thus, the present study explores the cytotoxic and cytostatic effects of LAECA.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Chromosome Complements and Karyological Studies in Medicago Species

    Rajpal, Vijay RaniRaina, Soom NathRao, Satyawada RamaSharma, Suman...
    6页
    查看更多>>摘要：The genus Medicago encompasses several significant and economically important annual and perennial taxa, each possessing distinct cytogenetical characteristics. Existing reports on chromosome numbers and karyology of the genus are quite intriguing and indicate the possible existence of underlying genetic variations. The present investigation on 18 taxa included three Lucerne varieties for the study of their chromosomal complements and karyological evolution. Twelve out of the 18 taxa analyzed were observed to be diploids with ten species having chromosome number of 2n= 16 and two, Medicago constricta and M. rigidula contained 2n= 14 chromosomes each. The remaining four species showed chromosome numbers of 2n= 30 and 2n= 32. Nucleolar organizing regions (NORs) have been a characteristic feature of the first pair of chromosomes in all the species analyzed. While the majority of the species analyzed show a basic number of x= 8, the polybasic nature of the genus becomes apparent with x=7 and 8 and occurrence of polyploid series with 2n= 14, 16, and 32. No heteromorphic pairs were observed in the chromosome complements of any of the analyzed species. This, along with the predominance of submetacentric chromosomes in the karyotypes validates the opinion that no significant structural changes have occurred to alter the karyotypes in the evolution of the genus. In the present study, numerical changes were encountered in M. arborea, M. scutellata, M. rugosa, and all three varieties of M. sativa.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Behavior of B-Chromosomes During Meiosis in Cousinia lactiflora Rech. f. (Cynaroideae, Asteraceae) from Iran

    Ghaffari, Seyed MahmoodGhaffari, Seyed-BehnamHejazi, Seyed Mohsen HesamzadehZare, Abbas Ghamari...
    4页
    查看更多>>摘要：Two accessions were recognized in the species of Cousinia lactifelora Rech. f. Accession A had n=12 and accession B showed the presence of 0 to 10 B-chromosomes at various stages of meiosis in addition to n=12 chromosomes. These B-chromosomes were smaller than the A-chromosomes and there existed as univalent and bivalents. Association of B-chromosomes with A-chromosomes occurred in less than 0.5% at metaphase I. This phenomenon is reported here for the first time. Analysis of chromosome behavior at meiosis showed that the presence of B-chromosomes decreases chiasma frequency in A-chromosomes. The effects of B-chromosomes in odd and even numbers are different. Comparison between plants with B-chromosomes and plants without B-chromosomes suggested that the effect of B-chromosomes was on pollen stainability. It seems that the presence of B-chromosomes may be responsible for the reduction of pollen stainability in C. lactiflora.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia

    Karyological Study of Five Species Boesenbergia (Zingiberaceae) in Thailand

    Saensouk, PiyapornSaensouk, Surapon
    6页
    查看更多>>摘要：The karyology of five species of Boesenbergia (B. collinsii, B. pulcherrima, B. rotunda, B. thorellii and B. xyphostachya) (Zingiberaceae) in Thailand are investigated. The chromosome number and karyotype for-mula of the five species are 2n= 20 (8m+ 10sm+2st) in B. collinsii, 2n= 36 (8m+18sm+10st) in B. pulcherrima, 2n= 38 (18m+14sm+6st) in B. rotunda, 2n= 38 (30m+8sm with one satellite) in B. thorellii and 2n= 22 (6m+6sm+ 10st with two satellites) in B. xyphostachya, respectively. Three species of B. collinsii, B. pulcherrima and B. thorellii have new chromosomal counts. The fundamental number (NF) and karyotype of all species in this study were reported for the first time. Satellites were observed in the chromosome of B. thorellii and B. xyphostachya.
      原文链接:
    • NSTL
    • Univ Tokyo Cytologia