Maria V. AlvanouDimitrios K. PapadopoulosAthanasios LattosIoannis Georgoulis...
14页
查看更多>>摘要：Abstract Freshwater crayfish are among the most important aquatic inhabitants in terms of ecosystem integrity retention. Although Astacus astacus and Pontastacus leptodactylus are characterized by the IUCN as vulnerable and least concern, respectively, Greek official data and local authorities report them as highly endangered or threatened at local levels. Therefore, all freshwater crayfishing activities from the main producing areas in Greece have been forbidden for 1?year since August 2021. Since aquaculture in Greece remains primarily focused on marine freshwater fish, freshwater crayfish aquaculture establishment is expected to provide a promising alternative for regional economic development and a solution to unemployment. Under this prism, the present study represents a comprehensive review of the most important features related to biology, distribution, aquaculture methods, economic importance and research progress considering both stocking perspectives and conservation strategies of those two crayfish species. Recent measurements by local authorities for the protection of the freshwater crayfish populations are described and evaluated. Freshwater crayfish aquaculture is expected to be beneficial for Greece, a country that covers all requirements for this sector. The updated information regarding these two species, summarizing the existing literature towards aquaculture conditions, poses suggestions and presents future perspectives on this field.
查看更多>>摘要：Abstract Cyanobacteria can form intense and sometimes toxic blooms in all kinds of aquatic ecosystems, typified as cyanobacterial bloom. Cyanobacterial blooms can create a range of harmful toxins, which may disturb water sources, successively posing serious health threat to living organisms, and at this stage they are termed as cyanobacterial harmful algal blooms (CyanoHABs). The occurrence of cyanobacterial blooms and cyanobacterial toxins are globally reported, so is the evident in Bangladesh, mainly trigged by increased anthropogenic eutrophication and global climate change. Cyanobacterial blooms and the accumulation of several toxins in human's drinking water, recreational water and aquatic biota such as zooplankton, fish larvae and juveniles interrupt aquaculture, aquatic ecosystem and public health in Bangladesh. As the management measures of toxic cyanobacteria and cyanotoxin are insufficient in Bangladesh, and the proper management for mitigating the worldwide occurrence of toxic cyanobacterial blooms is crucial for maintenance and sustainable development of functional ecosystems, some recommendations are illustrated. Further appropriate research on biology and ecology of cyanobacteria, cyanobacterial toxins and their relationship with environmental factors as well as nutrient management in fish ponds to manage noxious and harmful algae is essential to minimize their adverse impacts on aquaculture and public health for the country.
查看更多>>摘要：Abstract A 56‐day feeding trial was conducted to evaluate the effects of native and pregelatinized starches on feed quality, digestibility, growth, nutrient utilization, intestinal α‐amylase activity, plasma biochemical parameters and liver and intestine histology of Barbonymus schwanenfeldii juveniles. Six isonitrogenous and isocaloric diets were prepared using native and pregelatinized corn, sago and tapioca starches. Each diet was randomly assigned to triplicate groups of 20 fish (3.32?±?0.02?g). Results revealed an interactive influence of starch source and pregelatinization (p?<?0.05) on pellet expansion, sinking velocity, durability, water absorption index and water stability. However, the diets did not affect feed intake, growth and feed efficiency of fish. Accordingly, whole‐body composition, nutrient retention and body indices were also insignificant. Digestibility of dry matter, protein and carbohydrate depended on both starch sources and forms (p?<?0.05) while α‐amylase activity varied with starch sources and gut regions (p?<?0.05). Intestinal villus height of fish varied (p?<?0.05) with starch sources and forms, whereas villus width and area differed among combinations of starch sources and forms (p?<?0.05). Likewise, hepatocyte size of fish was different among dietary starch source and form combinations. However, the diets did not alter the plasma biochemical composition of fish. Overall, it was concluded that though pregelatinized starch improved pellet quality and nutrient digestibility, tinfoil barb juveniles could equally utilize 19.25% native and pregelatinized corn, tapioca and sago starches.
查看更多>>摘要：Abstract MINCLE is a C‐lectin receptor mainly expressed in macrophages to recognize carbohydrates enhanced by the innate immune response in mammals and fish. Toxin A (ToxA) from Vibrio parahaemolyticus N16 was evaluated as an antigenic protein; it was observed to induce IgM production, increase cytokine gene expression, and offer immunoprotection against bacterial challenges in fish. This study was aimed at analysing the MINCLE receptor from the Pacific red snapper (Lutjanus peru) through bioinformatics tools, and the stimulating effects of β‐glucans from Sterygmatomyces halophilus 4?N16 (β‐Glu/Sh) combined with an antigenic protein on head–kidney leukocytes (HKLs) before and on challenge with Vibrio parahaemolyticus N16. LpMincle was mainly expressed in the intestine, eyes and head–kidney tissues. Before and after V.?parahaemolyticus challenge, an up‐regulation of the receptors (Mincle, Marco and tlr2) and pro‐inflammatory cytokines (il‐1β and il‐6) gene expression was observed in HKLs incubated with β‐Glu/Sh. Respiratory burst activity was mainly enhanced in cells incubated with ToxA before and after V.?parahaemolyticus infection. Nitric oxide (NO) production increased in leukocytes incubated with β‐Glu/Sh or ToxA, whereas myeloperoxidase (MPO) activity augmented only after ToxA stimulation before the bacterial challenge. In conclusion, MINCLE from L.?peru was characterized in silico and its effects on HKLs was identified. Receptors and pro‐inflammatory cytokine genes up‐regulated by β‐Glu/Sh alone or in combination with ToxA can be elicitors of innate immune parameters. These findings evidence the function and recognition of novel β‐glucans from yeast either alone or in combination with antigenic proteins by β‐glucan receptors and their cytokines response.
查看更多>>摘要：Abstract The objective of this study was to investigate the potential of enhancing the dietary substitution of fishmeal (FM) with corn protein concentrate (CPC) in the diets of red hybrid tilapia, Oreochromis sp., by blending CPC with soy protein concentrate (SPC) and adding betaine‐HCl and dried basil leaves (DBL; feeding attractants). Seven isonitrogenous diets [FM100‐CPC0‐SPC0 (control); FM25‐CPC75‐SPC0 (75% of FM replaced by CPC); FM25‐CPC50‐SPC25 (50% and 25% of FM replaced by CPC and SPC, respectively); FM25‐CPC50‐SPC25B (with 0.5% betaine and 2% DBL); FM0‐CPC100‐SPC0 (100% diet protein from CPC); FM0‐CPC75‐SPC25 (75% and 25% of FM replaced by CPC and SPC, respectively) and FM0‐CPC75‐SPC25B (with 0.5% betaine and 2% DBL)] were formulated. Triplicate groups of tilapia fingerlings (average initial weight, 7.31?±?0.03?g) were fed one of the diets for 56?days. The effects of high dietary levels of plant protein concentrate (PPC) on growth, nutrient utilization, body composition, body colouration and gut histology of tilapia were investigated. High levels of dietary CPC significantly (p?<?0.05) reduced fish growth and feed intake. Blending CPC with SPC restored the growth performance of tilapia at the 75% PPC inclusion level. The addition of palatability enhancers further improved the growth of tilapia fed high PPC‐based diets.
Süleyman ?engülDevran Yazir?ebnem AtasaralMehmet Kocaba?...
11页
查看更多>>摘要：Abstract Turbot (Psetta maxima) is a commercially valuable species in aquaculture, and the new strategies and models for growth are essential for rapid growth in aquaculture operations. Hence, the objectives of this study were to assess the best feeding protocol and growth model for turbot in culture conditions. Over a period of 2?years, growth data were recorded for the determination of the most suitable growth model. Starvation and subsequent refeeding were realized in juvenile fish. The fish were exposed to three feeding regimes. Control groups (C1 and C2) were fed daily to satiation throughout the weeks 11 and 14. Fish in the two treatments were deprived of food for 1‐week followed by 2?weeks or 1 week of refeeding (T1 and T2) in repeated cycles. In the second experimental group, fish were deprived of food for 1?day followed by 2 or 1?day of refeeding (T3 and T4) in repeated cycles. Length and weight were determined at daily/weekly feeding (C2/C1), feeding every other day/week (T4/T2), feeding at two‐day/week intervals (T3/T1) in all treatments. The results indicated that growth in the T1 and T4 groups exhibited similar results with the control group. Overall, alternative feeding strategies can be used instead of continuous feeding.
Sheryll S. Santander‐Avance?aRex Ferdinand M. TraifalgarHarold M. MonteclaroJose Louis A. Castellano...
10页
查看更多>>摘要：Abstract Polychaete is considered the best maturation diet for penaeids; however, fluctuating supply and quality warrant detailed studies to understand the specific maturation‐promoting factors present in polychaete. Indian white prawn, Penaeus indicus, was fed diets supplemented with different fractions of Marphysa sp. extracts. Fractions, such as the total soluble fraction (TSF), neutral lipid fraction (NLF), and polar lipid fraction (PLF), were incorporated in the maturation diet at 0.25, 0.50, and 1.00% following a 3?×?3 factorial design. One group was fed with basal diet (BD), and another was fed with fresh squid, mussel, and polychaete, serving as the control. After a 30‐day feeding trial, results showed that the inclusion of polychaete extracts in the diet significantly improved P. indicus maturation compared to groups fed BD and control with 40% maturation rates (MR) (p?=?3.4?×?10?4). MR was optimum in groups receiving diets supplemented with ≥0.5% TSF (70.00%?±?0.00) and NLF (60.00%?±?5.77). Similar improved MR was achieved in treatments receiving ≥0.25% PLF supplementation (60%?±?0.00). Accordingly, relative expression of ovarian vitellogenin mRNA of broodstock fed under 0.25 and 0.50% PLF group was 4.44 and 3.96 folds higher than BD, respectively (p?=?0.003). No significant differences were detected in the broodstock survival, latency period, hepatosomatic, and gonadosomatic indices. Biochemical content analyses showed no significant differences among the nine treatments except for broodstock's higher ovary protein content in the TSF group (p?=?0.037). This study highlights PLF as the most potent component of the polychaete extract in promoting gonad maturation in P.?indicus maturation supplemented at a 0.25% optimum inclusion level.
查看更多>>摘要：Abstract The roe enhancement of sea urchins from barrens requires suitable transport techniques to enable transport of live sea urchins to enhancement facilities. For the green sea urchin Strongylocentrotus droebachiensis, the maximum period that the ‘out of water’ techniques described in this manuscript could be used is temperature dependent. When transported at 3.0°C, this should be no longer than 44‐hours, 36‐hours at 5.0°C and 14‐hours at an average temperature of 14.3°C. The survival results from the ‘in water’ transport system described in this manuscript indicate that this type of transport method will be suitable for transporting sea urchins for periods up to 22‐days and possibly longer if ammonia‐stripping (denitrifying) capacity is included in the transport system. The results of the post transport enhancement trial show that it is feasible to hold sea urchins at the densities tested for periods up to 14‐days and then to re‐immerse them in seawater holding systems and enhance the roe of the sea urchins successfully. There was a significant increase in gonad index in the sea urchins held in all the transport treatments compared to the initial wild GI, except in the sea urchins held at high density for 7?days. However, higher mortalities occurred in all transported sea urchins, and these were higher in sea urchins held at higher densities and for longer periods. The authors recommend the development of stress and welfare indicators for captive sea urchins to enable the ‘fitness’ of sea urchins during transport and captivity.
José Manuel Mazón‐SuásteguiGuadalupe Fabiola Arcos‐OrtegaCarlos Noé Contreras‐MendozaJesús Ramón Medina‐Sánchez...
13页
查看更多>>摘要：Abstract Most studies using Integrated Multi‐Trophic Aquaculture (IMTA) systems in shrimp aquaculture have assessed the feasibility of culturing non‐native bivalve species. However, studies including native species are limited and could represent a better alternative since these organisms are adapted to local environmental conditions and do not represent a sanitary risk. Therefore, we assessed the growth performance of the native oyster Crassostrea corteziensis in IMTA system with a Mexican shrimp farm. The study included three grow‐out cycles (starting in October, November and December), three stocking densities (low, medium and high), and three positions of the Nestier?‐like tray (top, middle and bottom), in a drainage channel connected to a shrimp farm located in a mangrove zone. From all experimental conditions, the larger wet weight (WW) and shell length (SL) values were registered in October grow‐out cycle, associated to warmer temperatures, from oysters at medium stocking density and at the top position (WW: 110.1?±?3.6?g; SL: 91.3?±?1.5?mm). These results suggest that grow‐out cycle, stocking density and position of oysters in the Nestier?‐like trays highly influence the performance of the cultured oyster. Additionally, C. corteziensis growth can be improved by using IMTA systems consisting of shrimp farm effluents.
查看更多>>摘要：Abstract Epidermal growth factor‐like domain protein (EGFL) 6, a member of the epidermal growth factor (EGF) domain protein superfamily, has been reported to regulate various physiological processes, including cell adhesion, proliferation, migration and inflammation in mammals. Up to date, the study of invertebrate EGFL6 expression pattern and functional analysis of EGFL6 involved in proliferation is lacking. In this study, a novel EGFL6 homologue was identified from Apostichopus japonicus (designated as AjEGFL6) by rapid amplification of cDNA ends. The full‐length cDNA of AjEGFL6 was 3915?bp, with a putative open reading frame of 2490?bp encoding 829 amino acid (aa) residue proteins. Structural analysis revealed that AjEGFL6 processed characteristic five EGF repeat domains and subclass of EGF_CA domain (89–338), UPF domain (504–607) and complement control protein (CCP, 755–808) domain. Multiple sequence alignment and phylogenetic analysis supported that AjEGFL6 belongs to a new member of the EGFL6 protein subfamily. Spatial expression analysis indicated that AjEGFL6 mRNA transcripts in the polian vesicle are expressed at a high level compared with that in coelomocytes. For Vibrio splendidus‐challenged sea cucumbers, the peak expression of AjEGFL6 mRNAs in coelomocytes was detected at 24?h (12.29‐fold), and it remained at high levels (1.52‐fold) until 72?h. Functional investigation via MTT and EdU assays revealed that 60 and 120?ng of recombinant EGFL6 administration significantly upregulated cell viability by 19.72% and 46.13% respectively. Taken together, these findings suggest that AjEGFL6 may serve as a coelomocyte proliferation regulator in the sea cucumber.
NSTL
Wiley