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Scientia horticulturae
International Society for Horticultural Science
Scientia horticulturae

International Society for Horticultural Science

0304-4238

Scientia horticulturae/Journal Scientia horticulturaeSCIISTP
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    Effect of hypobaric storage on Northland blueberry bioactive compounds and antioxidant capacity

    James, ArmachiusYao, TingMa, GuoweiGu, Zuchen...
    12页
    查看更多>>摘要:This paper studies the effects of hypobaric storage on the physiochemical quality of the Northland blueberry fruits, as well as their bioactive compounds, pre- and pro-antioxidant enzymes and reactive oxygen species (ROS). Different hypobaric pressures at 0.025, 0.05 and 0.075 MPa were applied to stored blueberry at 0 degrees C. Hypobaric treatment resulted in lower fruit decay rate compared to control, maintained respiration rate and pH of the fruit for 0.05 and 0.075 MPa treatments, which implied that fruit freshness was maintained following hypobaric storage. Also, hypobaric storage significantly retained high bioactive compounds (P < 0.05), especially total flavonol and anthocyanins after 50 days storage at 0.025 MPa compared to control. The retained bioactive compounds were reflected as high antioxidant capacity indicated by DPPH, FRAP and TEAC. The treatment 0.025MPa displayed the lowest MDA activity on day 23 and highest on day 50, which reflected delayed oxidation by hydrogen peroxide. Evidently, high levels of POD, APX, PPO and GSH antioxidant enzymes in the 0.025 MPa stored blueberries may be due to delayed ROS metabolism. Partial least square regression (PLSR) model showed that, ROS were negatively correlated with anthocyanin, POD, CAT, SSC, flavonol, TEAC, FRAP, DPPH as well as hydroxyl radical scavenging ability. Similarly, path analysis (PA) revealed that MDA was negatively correlated with TEAC, TA and fruit pH, while hydrogen peroxide was negatively correlated with GSH and protein. Of the pressures, 0.025 MPa was selected as the suitable hypobaric pressure for Northland blueberry storage as significantly retained studied indexes at relatively high values throughout 50 days storage.

    Foliar application of pig blood-derived protein hydrolysates improves antioxidant activities in lettuce by regulating phenolic biosynthesis without compromising yield production

    Zhou, WeiweiZheng, WenlongLv, HaofengWang, Qunyan...
    9页
    查看更多>>摘要:The application of natural biostimulants is proposed as an interesting approach to ensure sustainable yields and product quality in agricultural and horticultural crops. Here, the effects of foliar application of a protein hydrolysate derived from pig blood (Ppb) on biomass production, phenolic biosynthesis, and the correlation between phenolic profiles and antioxidant activities in lettuce were investigated. The Ppb application significantly enhanced the phenolic content and antioxidant properties in two genotypes of hydroponically cultivated lettuce by increasing the gene expression of enzymes (PAL, CHS, F3H, DFR, F35H, and UFGT) involved in phenolic biosynthesis. However, these changes occurred in a genotype-dependent manner. The results of HPLC and correlation analyses implied that the most contributory phenolic compositions to the increased antioxidant activities in Ppb-treated lettuce were flavonoids and anthocyanins. Meanwhile, no changes were observed in shoot and root fresh weights following Ppb application. These results clearly suggest that Ppb application is a useful strategy to improve antioxidant activities of lettuce by enhancing the biosynthesis of unique phenolic compounds without reducing plant yield.

    Drip irrigation with nanobubble oxygenated treated wastewater improves soil aeration

    Baram, ShaharWeinstein, MayaEvans, Jacob F.Berezkin, Anna...
    8页
    查看更多>>摘要:Long-term drip irrigation with treated wastewater (TWW) is known to enhance oxygen deficiency (hypoxia), especially in fine-textured soils. Hypoxia conditions around plant roots negatively affect growth and yield. Experiments were conducted to evaluate the response of lettuce grown in well-aerated (sandy) and poorly aerated (clayey) soils to surface and subsurface drip irrigation with nanobubbles oxygenated TWW (ONB-TWW). Oxygen mass balances show that irrigation with ONB-TWW supplied about 1% of the daily O-CO2 emissions. Nevertheless, our results show that both surface and subsurface drip irrigation with ONB-TWW increased the lettuce yield regardless of the soil type or the amount of oxygen added to the soil out of the daily oxygen consumption in it. Irrigation with ONB-TWW significantly reduced membrane leakage and osmotic potential in the roots and leaves in both well-aerated and poorly aerated soils, concomitantly improving root viability and chlorophyll content in the leaves of the plants grown in the poorly aerated clayey soil. The results suggest that drip irrigation with ONB-TWW, should be considered as a viable method to improve oxygen availability in soils and to alleviate soil hypoxia. Further study is needed to elucidate the mechanisms by which ONB promote plant health and growth.

    Quantifying the nitrogen allocation and resorption for an orchid pseudobulb in relation to nitrogen supply

    Zhang, WeiZhang, Shi-BaoFan, Ze-Xin
    9页
    查看更多>>摘要:As the main organ for storing water and nutrients, pseudobulbs are of central importance to the growth and survival of orchids. Quantifying nitrogen allocation and resorption of pseudobulbs is important for understanding the survival and adaptation strategies of orchids, but studies in this area are still lacking due to the complicated integration effect between pseudobulbs of different ages. In this study, nitrogen allocation to each organ and nitrogen resorption of Pleione aurita, an epiphytic orchid characterized by annually renewed pseudobulbs, were examined quantitatively under three nitrogen levels using 15N labeling. We found the old pseudobulb can act as a sink for exogenous nitrogen, although it had a much lower sink activity than the developing new pseudobulb. The single leaf accounted for the largest proportion of both exogenous and pseudobulb-stored nitrogen among all organs and in three nitrogen treatments, and the fruit tended to use pseudobulb-stored nitrogen preferentially regardless of exogenous nitrogen supply. The resorption of nitrogen in the leaf and pseudobulb was highly efficient, but decreased with increasing nitrogen supply. Overall, positive biomass accumulation was only observed in plants with exogenous nitrogen supply, while all fruit-bearing plants lost more than 30% of their biomass after a growing season regardless of nitrogen supply. The P. aurita plants exhibited a strong low nitrogen tolerance with only a slight loss of biomass for those nitrogen deficient and fruitless plants. Surprisingly, an increase in the nitrogen pool of the whole-plant was always observed regardless of biomass loss in fruit-bearing plants after nitrogen addition. Our results suggest an important role of the pseudobulb in the nitrogen economy of P. aurita, and the high dependence of the reproduction process on the pseudobulb-stored nitrogen. The findings will help to understand the adaptive strategies of orchids, and contribute to the conservation of endangered species.

    Nitrogen nutrition and fertilization of onions (Allium cepa L.)-A literature review

    Geisseler, DanielOrtiz, Roberto SotoDiaz, Jairo
    11页
    查看更多>>摘要:Nitrogen (N) is an essential nutrient, which often limits yield in crop production. Due to their sparse and shallow root system, the N fertilizer use efficiency in onion (Allium cepa L.) production is often low and the risk of nitrate leaching losses high. This review provides an overview of the scientific literature on efficient N management practices in onion and the effects of N availability on onion growth and bulb quality. Nitrogen demand of onions strongly depends on yield. On average 65% of the total N in the aboveground biomass is in the bulbs at harvest while 35% is in the leaves. The N concentration in the biomass decreases during the growing season. At harvest, bulbs contain on average 1.7 kg N Mg-1 total bulb yield. In addition to plant demand, the optimal fertilizer application rate depends on the availability of N from non-fertilizer sources, such as residual soil nitrate, nitrate in the irrigation water and N mineralized from organic material during the growing season. All of these sources are site-specific and can vary considerably. Therefore, recommended optimal N application rates need to be developed locally. There is no consensus on the best form of N fertilizer for onions. Differences in growth and yield found in individual studies appear to be due to the other ions included in the fertilizer materials and the effects of the fertilizers on the uptake of other nutrients and on soil properties. However, the N use efficiency can be improved with slow- or controlled-release fertilizers. Split applications can be timed to match the N available with crop N demand. This reduces the residence time of fertilizer N in soil and thus the risk of N being lost. Band application of N is another practice that can significantly increase yield and N uptake compared to broadcasting. Nitrogen availability can also affect bulb quality. Increasing N availability generally increases bulb size and decreases incidence of bolting. In contrast, increasing N availability, especially when it exceeds N demand, can contribute to a higher occurrence of thick neck, sprouting and bulb decay during storage. The overview of onion N use and N fertilizer management practices presented in this article can help choose optimal N fertilization practices based on site-specific conditions to achieve high N use efficiency and minimize losses.

    Uncovering the mechanism preliminarily of formation and development of taro corm in vitro by morphological physiology and transcriptomic analysis

    Zhu, QianglongLi, BicongLiu, XingyueShan, Nan...
    9页
    查看更多>>摘要:In vitro virus-free corms as propagules provide a feasible alternative to the convenient transport and the high survival rate of transplant for typical asexual crop of taro (Colocasia esculenta (L.) Schott). However, the mechanism of formation and expansion of in vitro corm remains unclear. In this study, in vitro corms were obtained from virus-free test-tube plantlet of taro in optimized induced medium (Murashige-Skoog medium + 2 mg L-1 6-Benzylaminopurine + 0.5 mg L-1 1-naphthalene acetic acid + 3% sucrose) (OIM), and then inoculated into the medium with Murashige-Skoog medium + 8% sucrose (MS8) for corm expansion. During this process, morphological observations by paraffin section, plant growth regulators (PGRs) content determination by enzyme-linked immunosorbent assay, and transcriptome assay by Illumina platform were carried out at days 0 (T0), 45 (T1), 90 (T2), and 135 (T3) under different corm expansion stages. The results showed that parenchymal cells enlarge continuously, accompanied with starch particles increase during corm expansion. Besides, three PGRs of indoleacetic acid (IAA), zeatin-riboside (ZR), and abscisic acid (ABA) were positively correlated with corm expansion, the contents of IAA and ABA remained at a high level. Moreover, the transcriptome results showed that plant hormone signal transduction and the starch and sucrose metabolism pathways are enriched significantly based on Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The genes (SBE2.2, GBSS, SUS4), related to starch biosynthesis were up-regulated, meanwhile, three genes of AMYs involved in starch metabolism were down-regulated, which probably promote starch accumulation and corms expansion. Eight genes from plant hormone signal transduction and the starch and sucrose metabolism pathway were selected randomly to validate the result of RNA-seq, and the RT-qPCR results were consistent with the RNA-seq data. These results will provide valuable information for uncovering the mechanism of in vitro corm expansion.

    Melatonin is a potential target for improving horticultural crop resistance to abiotic stress

    Zhao, ChenxuNawaz, GhazalaCao, QingheXu, Tao...
    9页
    查看更多>>摘要:Crop yield and quality are greatly affected by abiotic stresses, such as salinity, high and low temperature, drought and heavy metals, which pose major threat to human survival and development. Melatonin (N-acetyl-5methoxytryptamine), an indoleamine that widely exists in plants and animals, is a bio-stimulator involved in the regulation of plant growth and productivity. It scavenges oxidative stress caused by reactive oxygen species, potentially increases photosynthetic rate, chlorophyll content and is involved in the regulation of auxin, salicylic acid and nitric acid levels in plants. Genomic studies have explored melatonin-induced signaling, enzymes, transcription factors and the expression of stress- and metabolism-related genes in plants. Although much has been determined about the function of melatonin in plants, its role in inducing tolerance to abiotic stress in crop species is largely unknown. This review focuses on a comprehensive study of melatonin-induced physiological and molecular events in horticultural crops, which are important to the development of tolerance and enhancement of productivity in horticultural crops under abiotic stress conditions.

    Valeric acid suppresses cell wall polysaccharides disassembly to maintain fruit firmness of harvested 'Waizuili' plum (Prunus salicina Lindl)

    He, MingyangWu, YanfeiWang, YongHong, Min...
    7页
    查看更多>>摘要:Cell wall polysaccharides are closely related to fruit firmness. Firmness loss results in softening and restricts postharvest storage of plum fruit at ambient temperature. This study aimed to investigate the effects of valeric acid (VA) treatment on fruit firmness and cell wall polysaccharides metabolism in plum fruit. Results showed that 10 mg L-1 VA treatment delayed the decline of plum fruit firmness. VA-treated plum maintained higher contents of protopectin, cellulose, hemicelluloses, and lignin but lower water-soluble pectin (WSP) amount. Lower activities of polygalacturonase (PG), pectinate lyases (PL), pectinesterase (PE), 8-galactosidase (8-GAL), cellulase, hemicellulase but higher activities of phenylalanine ammonia lyase (PAL), 4-coumaroyl-CoA ligase (4CL) and peroxidase (POD) were observed in VA-treated fruit. These results suggested that VA treatment delayed the depolymerization of cell wall polysaccharides via inhibiting activities of degrading-enzymes. Moreover, lignin metabolism was regulated by VA treatment. Altogether, VA treatment regulated cell wall polysaccharides metabolism, then subsequently alleviated firmness loss and prolong storage-life of postharvest plum fruit.

    Comparative transcriptome analysis provides novel insights into the molecular mechanism of berberine biosynthesis in Coptis chinensis

    Liu, Xiao-MengTan, Jun-PingCheng, Shui-YuanChen, Ze-Xiong...
    13页
    查看更多>>摘要:Berberine is the primary medicinal component of Coptis chinensis, a perennial herb in Ranunculaceae. It has pharmacological effects, such as clearing away heat and dampness, purging fire and detoxifying, and resisting oxidation, and is widely used in clinical treatment. Anatomical analysis of the organs showed that berberine is mainly deposited in the xylem of the adventitious roots and rhizomes, as well as in thick-walled tissue layers containing vascular bundles in the leaves and petioles of C. chinensis. The content of total alkaloids in C. chinensis was highest in the rhizomes, among which berberine (54.44%) and coptisine (20.62%) were the main alkaloids. Furthermore, comparative transcriptome analysis of the leaves, petioles, rhizomes, and adventitious roots of C. chinensis was used to reveal the molecular regulation of alkaloid biosynthesis. A total 38 differentially expressed structural genes (DEGs) and 22 differentially expressed transcription factors were related to berberine biosynthesis in C. chinensis. Among these DEGs, the expression level of 13 genes was positively correlated with berberine content, including candidate structural genes (TYR, TYDC, TH, and PPO) and transcription factors (bHLH and WRKY), which may play key roles in berberine accumulation in C. chinensis. The results provide resources and technical support for improving the content of berberine in C. chinensis by genetic engineering, thus providing a theoretical foundation for enhancing the medicinal value of C. chinensis.

    The effect of hot water treatment on the storage ability improvement of fresh-cut Chinese cabbage

    Badelek, EwaSzczech, MagdalenaKosson, RyszardWrzodak, Anna...
    10页
    查看更多>>摘要:Fresh-cut Chinese cabbage (Brassica rapa ssp. pekinensis) was subjected to hot water treatment (HWT) at different temperatures (38-57 degrees C) and duration (3 s - 20 min.), followed by ambient air cooling. After treatment, the cabbage was stored at different temperatures: 0 degrees C, 5 degrees C and 15 degrees C. Fresh-cut cabbage (untreated) served as the control object. During storage, quality assessments and sensory evaluation were performed. Additionally, nutrient content and microbial contamination were determined. Chinese cabbage responded in a variety of ways to hot water treatment. Positive and stable reactions were obtained for cabbage after short treatment in a water bath at 53 degrees C and 55 degrees C for 3 s. These treatments delayed unfavourable quality alterations of fresh-cut cabbage, such as cut surface browning and rotting of leaf pieces at all storage temperatures. The development of off-odors and off-tastes was significantly inhibited in cabbage after HWT and short storage at 15 degrees C. In general, the treatments did not influence the nutritional value of the cabbage. The concentrations of ascorbic acid, total sugars, soluble phenols and antiradical activity did not differ significantly in treated and non-treated material. Only the content of nitrates in cabbage stored at 15 degrees C decreased significantly in all objects and the lowest amount was found for cabbage dipped in tap water. The intensive multiplication of yeasts and coliforms was noticed on fresh-cut Chinese cabbage after 3 days of storage at 15 degrees C. A lower storage temperature (0 degrees C) significantly inhibited microbial development in the cabbage, and especially yeasts and coliforms. The dipping of cut cabbage in tap water had no significant effect on the number of yeasts and coliforms, but increased mesophilic bacteria and Enterobacteriaceae compared to cut, untreated cabbage. HWT at 53 degrees C and 55 degrees C decreased the number of Enterobacteriaceae over 7 days of storage at 5 degrees C.