查看更多>>摘要：Cynara cardunculus L. is an Asteraceae member widely diffused in the Mediterranean Basin, rich in phenolic acids (caffeoylquinic acids and derivatives), flavones (luteolin, apigenin and their conjugates), anthocyanins, inulin and vitamin C. Thanks to their numerous biological activities, these compounds are in high demand for food and no-food applications. To match such request, in this research we evaluated the effect of three water-supply (100%, 75% and 50% of plant requirement) and light (24 h, 12 h and 0 h) treatments on the polyphenols profile of C. cardunculus seedlings, with the aim of developing a production system under controlled conditions. Overall, the 100% of plant water requirement increased the amount of caffeoylquinic acids (+28%), luteolines (+27%) and total measured polyphenols (+26%) respect to water-stressed plants (75% and 50% of plant requirement), with cultivated cardoon showing a higher concentration than the globe artichoke. Concerning the light treatment, the trend 0 < 12 < 24 h was found for all phytochemical compounds. In particular, 24 h of light strongly induced the biosynthesis of caffeoylquinic acids (+119%), luteolines (+273%) and total measured polyphenols (+129%) compared to 0 h of light. In both experiments, the most abundant compounds were 5-O- caffeoylquinic acid and 1,5-O-dicaffeoylquinic acid. Regardless of experiment, the genetic background showed a significant role, since the responses were genotype-dependent. From these results clearly emerged the possibility of producing polyphenols-enriched C. cardunculus seedlings in controlled conditions.
查看更多>>摘要：Anthocyanins have been found to be responsible for the pigmentation of red, purple and blue colors in plants. MicroRNA (miRNA) plays an important role in the regulation of anthocyanin biosynthesis in plants. However, few miRNAs and their corresponding functions related to anthocyanin biosynthesis in ornamental kale (Brassica oleracea var. acephala) have been reported. In this study, small RNA (sRNA) and degradome libraries from inner and outer leaves (IL and OL) of ornamental kale line "Pigeon Red" were constructed, respectively. Totally, 139 known and 14 novel miRNAs were identified by sRNA sequencing, and 304 target genes cleaved by 92 bomiRNAs were identified by degradome sequencing. Of these, 5 miRNAs and their corresponding targets were potentially involved in the anthocyanin biosynthesis. And 35 miRNAs were differently expressed between IL and OL. Integrated analysis of sRNA-Seq, degradome sequencing and RNA-Seq revealed that bo-miR828 and its targets potentially play a key role in the regulation of anthocyanin biosynthesis in ornamental kale leaves. Finally, an inverse correlation between the expression of bo-miR828 and its targets was revealed by both qRTPCR and high-throughput sequencing. These results represented a comprehensive expression profiling of miRNAs related to anthocyanin accumulation in B.oleracea var. acephala and provided important clues for understanding the regulatory network of anthocyanin biosynthesis mediated by miRNA in Brassica crops.
NSTL
Elsevier