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Scientia horticulturae
International Society for Horticultural Science
Scientia horticulturae

International Society for Horticultural Science

0304-4238

Scientia horticulturae/Journal Scientia horticulturaeSCIISTP
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    Major gene with polygene inheritance analysis of shoot architecture traits in Viola cornuta

    Du X.Wang H.Liu J.Zhu X....
    8页
    查看更多>>摘要:? 2022The shoot architecture of Viola cornuta affects its uniformity, aesthetics, and lodging resistance. Understanding the genetic variance in the traits of the shoot architecture has practical significance for V. cornuta breeding. However, not much is currently known about these traits. In this study, we analyzed the genetic basis of seven traits (plant height-PH, plant width-PW, plant width/plant height-PW/PH, internode length-IL, shoot number-SN, shoot angle-SA, and pedicel length-PL) in V cornuta using major gene plus polygene mixed inheritance analysis based on four generations (P1, P2, F1, and F2). These seven traits had varied coefficients of variation (CVs), among which that of the IL was the highest. Significant positive correlations (correlation coefficient > 0.5) were observed for two groups: 1) PH and PW/PH and 2) IL and PH and PW. Genetic analysis revealed that V. cornuta PH adhered to the A-1 model via one additive major gene. The optimal model for PW, PW/PH, and SA was the B-4 model, namely two equally additive major genes model. IL, SN, and PL could be described by the B-2 genetic model via two additive major genes. Additionally, the heritability of these major genes was close to (and sometimes exceeded) 50%, indicating that the shoot architecture traits of V. cornuta were mainly controlled by genetic factors. Our findings may provide new theoretical data for quantitative trait locus mapping and breeding of desirable shoot architectures in V. cornuta.

    Effect of moderate salinity on Golden Thistle (Scolymus hispanicus L.) grown in a soilless cropping system

    Daliakopoulos I.N.Kontaxakis E.Sabathianakis M.Manios T....
    11页
    查看更多>>摘要:? 2022Selecting salt-tolerant crop species for cultivation might be a feasible option to maintain crop productivity in arable lands affected by salinity. In this context, here we examine the responses of the wild edible green golden thistle (Scolymus hispanicus L.) to moderate levels of salinity when grown in a soilless system for culinary use. Treatments were obtained by establishing four NaCl concentrations (0.5, 5.0, 10.0, and 15.0 mM) in a standard nutrient solution, resulting in initial solution electrical conductivities (EC) of 2.2, 2.8, 3.2, and 3.8 dS m ? 1, respectively. By the end of the experiment, 120 days after transplanting (DAT) drainage EC reached 2.65, 3.33, 3.96, and 4.51 dS m ? 1, respectively. At the first harvest, 90 DAT, the increase of the NaCl concentration had no significant impact on the number of leaves per plant, leaf fresh and dry weight [g plant?1], and root dry weight [g plant?1]. At the second harvest (120 DAT), 15.0 mM NaCl significantly decreased root and leaf fresh and dry weight without affecting the number of leaves per plant compared to 0.5 and 5.0 mM NaCl, while 10 mM NaCl had no impact on any of these parameters. The concentrations of K+, Ca2+, Mg2+, and P in the leaves were not influenced by the tested salinity levels, while those of organic-N and NO3?-N were significantly reduced by 10 and 15 mM NaCl, 90 DAT. Furthermore, 10 and 15 mM NaCl significantly increased the Cl? and Na+ concentrations in leaves, but not in the roots. In the root tissues, only the K concentration was reduced by salinity while P increased at the highest salinity level (15 mM NaCl). Based on these results, we conclude that S. hispanicus L. is resilient to moderate salinity levels and can be considered a promising candidate plant for introduction to soilless cropping systems.

    Bioactive constituents of Allium vineale L. accessions from Eastern Turkey

    Cakmakci O.Sensoy S.Alan A.R.
    8页
    查看更多>>摘要:? 2022Edible parts of Alliums are rich sources of health promoting bioactive compounds such as polyphenols, flavonoids, and antioxidants. These compounds are known to promote human health by reducing the risk of various cancer, cardiovascular and brain and diabetes diseases. Allium vineale is an edible wild Allium species collected from nature and used in herbed cheese production. Quality of herbed cheese is associated with A. vineale leaves, the main ingredient used as fresh or dried in the process of cheese production. The aim of this study was to evaluate bioactive constituents in above ground organs of 10 accessions from an A. vineale germplasm collected from Van province of Turkey. Each accession was represented with 10 plants in the analyses for bioactive compounds. It was found that A. vineale accessions were significantly different from each other for their bioactive content levels. Statistically significant differences were found among the accessions for total phenolic content and antioxidant activities. On average, A. vineale samples contained 60.11 g GAE kg?1 total phenolic compound and 98.84 mmol TR kg?1 total antioxidant capacity. The main phenolic compound detected in A. vineale was protocatechuic acid (~221 g kg?1). The findings obtained from this study provide an opportunity for selecting genetically superior accessions with high bioactive compound production as starting materials in the improvement of new A. vineale cultivars suited for controlled cultivation.

    Identification and expression analysis of AP2/ERF superfamily in pecan (Carya illinoensis)

    Liu H.Wang L.Lan Y.Wu M....
    15页
    查看更多>>摘要:? 2022 Elsevier B.V.AP2/ERF is a large transcription factors superfamily whose members are participated in the control of plant development, metabolism and response to various biotic and abiotic stresses. Nevertheless, few studies have been reported in pecan. In this study, a total of 170 CiAP2/ERF members were identified and divided into 4 main subfamilies, AP2 (16 genes), RAV (6 genes), Soloist (1 gene), as well as ERF (147 genes) families, and ERF subfamily was further classified into group I to VI-L. Gene structures and conserved motifs indicated that individual groups were highly conservative. A large number of cis-elements such as Box 4, ABRE, and MYB were detected in the promoter region of CiAP2/ERF genes. Transcriptional levels in four tissues were analyzed using available transcriptome data. qRT-PCR analysis of the ERF VII group revealed that the subfamily had a positive response to waterlogging stress. The subcellular localization experiments on CiAP2/ERF21, CiAP2/ERF65, and CiAP2/ERF106 manifested that they were localized in nuclear. Additionally, CiAP2/ERF65 and CiAP2/ERF106 had the ability of self-activation in yeast, while the CiAP2/ERF21 gene did not. These results would conduce to understanding the AP2/ERF superfamily and functional roles of CiAP2/ERF TFs under different stresses.

    Productive performance of ‘Pera’ sweet orange grafted onto 37 rootstocks in tropical cohesive soils under rainfed condition

    Passos O.S.Filho W.D.S.S.de Carvalho H.W.L.de Carvalho L.M....
    11页
    查看更多>>摘要:? 2022 Elsevier B.V.In tropical cohesive soils of Brazil, ‘Rangpur’ lime provide high fruit yield to ‘Pera’ sweet oranges. However, the predominance of a single rootstock puts the citrus industry in this region at risk. To identify promising rootstocks for diversification of ‘Pera’ sweet orange orchard's in the tropical cohesive soils of Brazil, a trial was established with 37 citrus rootstocks on the northeast coast of Brazil. The horticultural performance, fruit yield up to the tenth year, and physic-chemical fruit quality attributes were evaluated. The rootstocks induced changes in the tree size, cumulative fruit yield fresh mass and size of fruit, but had not significant influence on chemical fruit quality. The Hierarchical Cluster Analysis allowed group rootstocks into three classes, in which the accumulated fruit yield was the attribute that most contributed. A group with 15 rootstocks was chosen due to the best productive performance and fruits with 193.75–218.5 g, 57.36–62.65% of juice with 9.85–13.08° brix, which meets the requirement of the Brazilian market. Among these 15, the hybrid of ‘Volkamer’ lemon (VKL) with ‘Rangpur’ lime (RL), ‘Maravilha Sunki’ mandarin, ‘Indio’ citrandarin and the local selection of RL stood out by inducing the highest yield. VKL × RL – 030 produced fruits later, but favored the highest fruit yield at the tenth year. Trees grafted on these four rootstocks and also onto RLCNPMF, TSKC × CTQT1434 – 001, MXWL × LHA – 004, ‘Santa Cruz’ Rangpur, ‘San Diego’ citrandarin,– 004 and trifoliate TRH-208 had greater production regularity across the harvests, although the last three showed lower cumulative yield. The earliest fruit bearing was induced by TSKFL × CTC13 – 012, followed by the ‘Sunki' mandarin hybrids TSKC × CTQT1434 – 001, RL, TSKC x TRFD -007 and TSKC x CTSW-041. It is concluded that the most promising rootstocks for diversification of citrus orchards under rainfed condition on the northeast coast of Brazil are VKL x RL-030, ‘Maravilha Sunki’, ‘Indio’, TSKFL × CTC13 – 012 and TSKC × CTQT1434 – 001, RLCNPMF – 004, TSKC x TRFD -007, TSKC x CTSW-041 and MXWL × LHA – 004.

    Bioactive copper and Bacillus subtilis for the control and resistance induction against citrus canker in sweet orange [Citrus sinensis (L.) Osbeck] orchard establishment

    Ramos Y.G.Duin I.M.da Silva M.R.L.Junior R.P.L....
    12页
    查看更多>>摘要:? 2022Resistance is the plant ability to prevent or delay the infection and the development of a pathogen. The objective of this study was to determine the efficiency of treatments with chemical and biological products for the control of citrus canker during the establishment of citrus orchards through resistance induction. The treatments consisted of a combination of foliar and drench applications of products based on Bacillus subtilis (Bs), bioactive copper (BioCu), copper hydroxide (HCu) and acibenzolar-S-methyl (ASM), at the transplanting of the nursery trees to the field and throughout the first year of establishment of the citrus orchard. Evaluations were carried out on mature branches determining the incidence of citrus canker, defoliation, and citrus leafminer (CLM) injures. The vegetative growth of the citrus trees, i.e. tree height and trunk and canopy diameters, were also determined. Under controlled conditions, studies were carried out to determine the activation of the systemic acquired resistance (SAR) by treatments with ASM and Bs applied via drench, and by BioCu and copper sulfate (SCu) applied by spraying on the leaves of the citrus trees. The expression of the PR-2 gene was evaluated by RT-qPCR at 24, 42, and 72 h, and seven days after the application of the treatments. In the field, the ‘IAPAR 73′ sweet orange trees treated via drench with Bs and foliar applications of Bs + HCu, Bs + BioCu and HCu with and without ASM showed reductions in the incidence of citrus canker. HCu treatments reduced defoliation, while foliar treatments with Bs decreased the incidence of CLM injures. The ‘IAPAR 73′ sweet orange trees treated with ASM showed a reduction in the vegetative growth. The BioCu and Bs products were effective for the control of citrus canker in young citrus trees during the orchard establishment. In addition, the BioCu product increased the expression of the PR-2 gene, which encodes the β-1,3 glucanase, a protein associated with the systemic acquired resistance (SAR) in plants.

    Penicillium growth inhibition, fruit decay reduction, and polymethoxyflavones and scoparone induction in satsuma mandarin irradiated with ultraviolet-A light-emitting diodes

    Yamaga I.Nakamura S.
    9页
    查看更多>>摘要:? 2022In this study, we investigated the effects of ultraviolet (UV)-A light-emitting diodes (LEDs) on the inhibition of fungal spore germination, fruit decay, and accumulation of secondary metabolites, including phytoalexins, in satsuma mandarin. We examined the growth and development of blue (Penicillium italicum) and green (Penicillium digitatum) molds exposed to UV-A LED irradiation in vitro and found that UV-A LEDs with an emission peak of 375 nm and light intensity of 9.4 W m?2 significantly reduced the growth of both these fungi. In addition, wounded fruits inoculated with P. digitatum were irradiated with UV-A LEDs for 6 days, and we monitored the subsequent changes in green mold symptoms. The results indicated that low-intensity UV-A LED treatment significantly reduced green mold symptoms in fruit, being the effect most pronounced inhibitory on Penicillium sporulation. Furthermore, wounded fruits subjected to UV-A LED treatment were found to have significantly higher contents of the coumarin compound scoparone than fruits maintained in the dark. Contrastingly, scoparone was barely detectable in non-wounded fruits, irrespective of UV-A LED irradiation. The contents of nobiletin, tangeretin, and hesperidin were likewise found to be higher in wounded fruits treated with UV-A LED than in either non-wounded or non-irradiated fruits. These results indicate that treatment with low-intensity UV-A LED irradiation is sufficient to reduce mold symptom development, and accordingly could represent a promising safe approach to the control of post-harvest decay in citrus fruits. On the basis of our observations, we speculate that the biosynthesis of scoparone, polymethoxyflavones, and flavanones could be induced when the peel is injured and irradiated with UV-A contributing to a reduction in fruit decay.

    Different miRNAs and hormones are involved in PEG-induced inhibition of adventitious root formation in apple

    Zhang X.Khan A.Ma J.Tahir M.M....
    17页
    查看更多>>摘要:? 2022 Elsevier B.V.In apples, adventitious root (AR) formation plays a crucial role in the life cycle, but drought stress limits this process. Multiple hormones and miRNAs play key roles in regulating ARs under drought conditions, but they are lacking in GL-3 apple clones. Therefore, to identify physiological and molecular changes behind the phenotype, GL-3 apple cuttings were treated with 6% polyethylene glycol (PEG) during the activation, induction, initiation, and emergence stages of AR formation and compared with a PEG-free control. Anatomical analysis indicates that 6% PEG treatment delays AR primordia formation and produces fewer thin ARs than control plants by decreasing the indole-3-acetic acid (IAA) endogenous content and promoting the abscisic acid (ABA), methyl jasmonate (JA-me), and zeatin riboside (ZR) content at all time points. Furthermore, 32 known miRNAs belonging to 22 families and 60 novel miRNAs were found to be differentially expressed. A total of 81 target genes of known miRNAs and 62 targets of novel miRNAs were identified using degradome sequencing. The known miRNA targets were classified into 50 GO terms. In biological processes, various terms related to the response to water deprivation; salt, heat, and cold stress; abscisic acid-activated signaling pathways; and so on, were observed. The KEGG analysis indicates 20 pathways from which plant hormone signal transduction, ribosomes, etc., were the most enriched. Further analyses indicated that miRNAs (miR167b, miR393g, and miR477a) related to IAA, ABA, and JA were induced; various stress-responsive miRNAs (miR171c, miR169o, miR159a, miR10979, and miR167a) were also promoted; and cell division and development-related miRNAs (miR396a and miR482a) were repressed by 6% PEG treatment. These results indicate that AR formation is an intricate biological process in apples under stressed conditions regulated by various hormones, related miRNAs, and target genes. This study delivers new information about the underlying regulatory mechanisms of AR formation in GL-3 and other plants.

    Glycinebetaine facilitates the photosynthesis of albino tea under low temperature by regulating related gene methylation

    Huang S.Yao L.Luo L.Zuo T....
    9页
    查看更多>>摘要:? 2022The frequent occurrence of adverse weather conditions in early spring caused by global climate change has seriously restricted the physiological metabolism and growth of plants in recent years. As a low temperature sensitive albino tea cultivar, white leaf No. 1 (WL-1) has been seriously affected. Regulation of DNA methylation (an epigenetic regulator) due to global climate change by glycinebetaine (GB, a bioactive methyl donor) has been often observed in animals/humans, but little is known about whether it is linked to gene methylation priming responses of plants to low temperature. Herein, the effects of foliar application of GB on photosynthesis, photosynthetic pigment synthesis and related genes methylation of WL-1 functional leaves under low temperature were investigated. Results showed that the transpiration efficiency and chlorophyll contents of WL-1 functional leaves under low temperature condition were significantly improved by the application of GB (P < 0.05), and the net photosynthetic rate was increased by 45.3%. In addition, the application of GB promoted the methylation of most methyltransferase and demethylase genes, PSⅡ component genes and some chlorophyll synthesis genes in WL-1 functional leaves under low temperature. It was verified that the changes of photosynthetic parameters and chlorophyll content may be correlative to related gene methylation. Together, present study suggests important roles of GB in facilitating photosynthesis of albino tea functional leaves under low temperature by regulating related gene methylation.

    Season and genotype effect on whole plant water use efficiency of coconut (Cocos nucifera L.) seedlings grown in a hydroponic system

    Hebbar K.B.Kannan S.Sukumar. P A.Ramesh S.V....
    11页
    查看更多>>摘要:? 2022 Elsevier B.V.A study was conducted to understand the influence of climatic factors on nutrient use, and whole plant water use efficiency (WUE) of hydroponically grown coconut seedlings. Two dwarf (Chowghat green dwarf, CGD and Malayan yellow dwarf, MYD) and two tall (Kalpa Pratibha, KP and Kalpatharu, KT) coconut genotypes were grown in triplicates and their water use, and biomass production were measured and WUE was analyzed across summer, monsoon and winter seasons, characterized with distinct climatic variability. In comparison to monsoon, during summer the morning temperature was (6 ℃) high, and relative humidity (RH) was low during summer resulting in two folds increase in vapour pressure deficit on leaf surface (VPDL). Increasing VPDL had direct influence on water consumption of the plant per day which was significantly high during summer (3.14 L) compared to monsoon (1.83 L), but the difference in biomass gain was not significant. This had led to a weakly negative [R2(144) =0.12, p <.0001] relation between water use and WUE, the WUE was only 3.35 g L?1 during summer as against 6.6 g L?1 of monsoon. The response of genotypes to increasing VPDL was also significantly different. The sensitive stomata of talls could conserve water and maintain significantly high WUE while insensitive stomata of dwarfs allowed greater water loss without a concomitant increase in biomass resulted in low WUE. The early photosynthetic light saturation at 1400 μ mole photon m?2 s?1 might have also contributed to low biomass production in dwarfs. This study divulges that, at high temperature and low humidity in general, coconut WUE is low and especially dwarfs are not suitable for those regions with dry weather or regions expected to become dry under future climate scenario.