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Industrial Crops and Products
Elsevier Science Publishers
Industrial Crops and Products

Elsevier Science Publishers

0926-6690

Industrial Crops and Products/Journal Industrial Crops and ProductsSCIISTPEI
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    Comparative full-length transcriptome analysis provides novel insights into the regulatory mechanism of natural rubber biosynthesis in Taraxacum kok-saghyz Rodin roots

    Qin, BiChen, QiuhuiZhang, JichuanZhang, Liqun...
    11页
    查看更多>>摘要:Taraxacum kok-saghyz Rodin (TKS) is a promising alternative rubber-producing plant due to the high-quality natural rubber (NR) synthesized in its root. Nevertheless, the comprehensive molecular mechanisms of NR biosynthesis in TKS roots remains segmented and elusive. In the current study, to elucidate the regulatory mechanism of NR synthesis at the transcriptional level, high-quality full-length root transcriptomic data of the TKS were generated based on Oxford Nanopore Technology (ONT). The transcripts with alternative splicing events, polyadenylation sites, and simple sequence repeats (SSRs) were screened. Then, comparative transcriptome analysis was conducted between the lines containing high rubber (HR) and low rubber (LR) contents, and a total of 3134 differentially expressed genes (DEGs) were identified. Among the genes, 1157 DEGs were upregulated, and 1977 DEGs were downregulated in the HR lines vs. the LR lines. The genes involved in NR synthesis were upregulated in HR lines. In contrast, the key genes involved in the synthesis of squalene, phenylpropanoid, fatty acids and ketone, which require the same substrates (isopentenyl pyrophosphate or acetylCoA) as NR biosynthesis, were downregulated in HR lines. The results indicated that the expression levels of NR synthesis-related genes were proportional to the NR production, and competition between NR and some metabolites synthesis existed when common substrates were used. Hence, this work provides novel insights into the regulatory mechanism of NR biosynthesis in TKS. Furthermore, the genes or pathways related to NR synthesis identified in this study will be important targets for genetic modification and molecular breeding in further.

    Nano silver decorating three-dimensional porous wood used as a catalyst for enhancing azo dyes hydrogenation in wastewater

    Yu, YuanyuanHuo, HuashuangZhang, QingtongChen, Yangyang...
    9页
    查看更多>>摘要:The natural wood can be used as a multifunctional material e.g. a reducing agent and a stabilizer due to the reducing functional groups and three-dimensional network structure. In this work, a wood catalyst decorated with silver nanoparticles (Ag NPs) was prepared and used to catalyze the hydrogenation of azo dyes. The systematic characterizations revealed that the Ag NPs were uniformly present on the wood surface. The Ag NP/ wood exhibited an excellent catalytic activity for hydrogenating methyl orange (MO), congo red (CR) and erichrome black T (EBT), and their reaction rate constants were calculated as 1.533, 0.969 and 0.707 min-1, respectively. The factors affecting the hydrogenation efficiency of azo dyes were elucidated by the density functional theory (DFT). The catalyst had a good reusability, for instance the hydrogenation efficiency of MO retained as 90% in 5 min after six recycles. In addition, the mechanism of MO hydrogenated by NaBH4 catalyzed with the Ag NPs/wood was proposed.

    Oxidative coupling of Kraft lignin mediated with hypervalent iodine reagent (III) for enhanced removal of dye in water

    Chen, QinPeng, ChangLiu, WujunNing, Siyang...
    11页
    查看更多>>摘要:One of the most attractive routes to valorize lignin is to prepare lignin-based materials which can be used as high-value products, such as dye absorbents. Various processes have been investigated to improve the properties of lignin. In this study, a hypervalent iodine reagent (III) was employed to achieve oxidative coupling of Kraft lignin (KL). The structural changes were investigated by FTIR, quantitative P-31 NMR, 2D-HSQC, and GPC. Oxidative coupling between lignin aromatic C(sp(2))-H bonds and aromatic C(sp(2))-H functionalization with aliphatic hydroxyl group occurred via inter- or intramolecular modes, with resultant decreases of the aromatic protons and hydroxyl groups. In addition, selective modification of aliphatic OH groups in beta-O-4' linkages occurred leading to the formation of aromatic carbonyl groups. Increases in molecular weights with the appearance of conjugated C=O groups were also observed. The oxidative crosslinked lignin (KL-2.0) exhibited better adsorptive features for brilliant green (251.2 mg/g) than that of unmodified KL (59.4 mg/g). Their adsorption kinetics were investigated by pseudo-first-order, pseudo-second-order and mixed-order models. Furthermore, the Langmuir and Freundlich models were employed to study the adsorption isotherms.

    The use of widely targeted metabolomics profiling to quantify differences in medicinally important compounds from five Curcuma (Zingiberaceae) species

    Ye, YuanjunZhang, XiaoniChen, XueqingXu, Yechun...
    8页
    查看更多>>摘要:The genus Curcuma is widely recognized for its diversity of medicinal and culinary uses yet metabolomic dif-ferences among Curcuma species are largely unknown, due to the lack of broadly targeted analytical studies. Here, Ultra-Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (UPLC-MS/MS) anal-ysis was employed to interrogate the metabolomes of five Curcuma species which are commonly used in Chinese herbal medicine (C. aromatica, C. elata, C. longa, C. caesia and C. phaeocaulis). By processing these results through a pathway enrichment analysis, 432 metabolites (some associated with the curcumin pathway) were identified that varied by species. The quantity of curcuminoids in C. longa were found to be higher than that in the other four Curcuma species analyzed in this study. In addition, C. longa was found to have a greater diversity of phenolic acids, amino acid derivatives, and flavonoids associated with the curcumin biosynthesis pathway. However, medicinal compounds such as 6-gingerol were found in lower quantities in C. longa compared to all other species in this study. This study provides new insights into Curcuma phytochemical pathways and allows for the development of functional foods tailored to specific needs from these species.

    Ginsenosides accumulation and related genes expression in different underground parts of Panax notoginseng during maturation stage

    Tang, QingyanLu, YingchunZhang, GuanghuiTang, Junrong...
    12页
    查看更多>>摘要:Ginsenosides, which mainly exist in rhizomes and roots of Panax notoginseng (Burk) F. H. Chen, exhibit various physiological functions and medicinal values. However, the accumulation process of ginsenosides in the rhizome, epidermis, and xylem of the main root is unknown. Herein, the temporospatial distribution and mechanism of variation of ginsenosides in the underground parts of three-year-old P. notoginseng were investigated. The results showed that the accumulation of ginsenosides in rhizomes accelerated from May. In 12-month growth stages (except May), the content of ginsenosides in the xylem and epidermis were higher than that in the rhizome. However, the ginsenosides in rhizomes increased rapidly, whereas the ginsenosides in the main root decreased from May to July. The expression levels of genes encoding squalene epoxidase, dammarenediol synthase, and uridine diphosphate-dependent glycosyltransferase (UGTPg1) in rhizomes were increased in May, whereas genes encoding squalene synthase, protopanaxadiol synthase, protopanaxatriol synthase, UGTPg45, and UGTPg29 were expressed at considerably increased levels in June. Distinct expression levels of genes related to ginsenoside biosynthesis were the main reasons for the temporospatial fluctuation of ginsenoside accumulation in the two tissues. weighted gene co-expression network analysis demonstrated that many transcripts regulated ginsenoside accumulation in underground tissues. These results provide essential data for farmers and processors participating in P. notoginseng cultivation and utilisation.

    Irrigation with ionized brackish water affects cotton yield and water use efficiency

    Wei, KaiZhang, JihongWang, QuanjiuGuo, Yi...
    12页
    查看更多>>摘要:Irrigation agriculture, which is commonly applied in the arid area in Northwest China, faces multiple threats, including drought, shortage of freshwater supply to irrigation districts and soil salination. Reducing salinity damage to plant growth and, improving irrigation water productivity with ionized brackish water are crucial for sustaining the agricultural production in the region. In the current study, we determined the responses of cotton (Gossypium hirsutum L.) growth, yield and, water productivity for several irrigation amounts and ionized treatments at southern Xinjiang, China. We applied five drip irrigation treatments: (W1, 262.5 mm; W2, 337.5 mm; W3, 412.5 mm; W4, 487.5 mm; and W5, 562.5 mm) of non-ionized and ionized brackish water, resulting in total of 10 treatments, (NIW1, NIW2, NIW3, NIW4, NIW5, IW1, IW2, IW3, IW4 and IW5). The experimental design incorporated 10 irrigation treatments in a randomized block design with four replicates. Results demonstrated the improvements in plant height, leaf area index, shoot dry matter, boll number per plant and chlorophyll content resulting from the ionized treatment. Moreover, the ionized treatment effectively reduced salt accumulation amount from late seedling stage to harvest by 12.8%-65%, especially in 562.5 mm of ionized brackish water treatment. The maximum cotton yield was obtained in 487.5 mm of ionized brackish water treatment, with increases of an average of 19% compared to 487.5 mm of non-ionized brackish water treatment. Water use efficiency increased significantly under the ionized brackish water treatments. A quadratic relationship was observed between irrigation amount and the cotton yield. Based on the soil salt, cotton yield and irrigation water use efficiency, the irrigation amount was optimized at 487.5 mm for ionized brackish water-drip irrigation plastic film mulching systems. This study provides valuable guidance on the use of brackish water irrigation to improve water productivity while maintaining an acceptable cotton yield in the arid area.

    Fine-tune of lignin properties by its fractionation with a sequential organic solvent extraction

    Izaguirre, NagoreRobles, EduardoLlano-Ponte, RodrigoLabidi, Jalel...
    12页
    查看更多>>摘要:In this work, different lignins were obtained from two different extraction methods (kraft and organosolv) but from the same raw material (Eucalyptus globulus sp.). They were subsequently fractionated to determine the differences of each extraction method and their corresponding physicochemical properties found in fractionation sequence and obtained fractions. The goal of the fractionation was to obtain lignin fractions with narrower molecular weight distribution and lower polydispersity index (PI). The solvent sequence was designed based on the environmental friendly properties, health and safety assessments of the selected organic solvents: (methanol (MeOH), ethanol (EtOH), propan-2-one (DMK), ethyl acetate (EtOAc), propan-1-ol (nPrOH), propan-2-ol (iPrOH), butan-2-one (MEK), and butan-1-ol (tBuOH)). The different fractions obtained were characterised to determine their chemical structure by several analytical techniques, such as Fourier Transformed Infrared Spectroscopy (FTIR), Ultraviolet (UV), Phosphorus-31 Nuclear Magnetic Resonance (P-31 NMR), Pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS), Thermogravimetric analysis (TGA), and Differential scanning calorimetry (DSC). In addition, Gel Permeation Chromatography (GPC) was used to obtain the molecular weight distribution. This study showed an effective method for obtaining homogeneous lignins with specific structures and properties depending on the solvent and molecular weight attained. Moreover, the method designed was found to be effective regardless of the lignin extraction process employed; besides, various lignin fractions were obtained which were different from each other, having specific target applications depending on their structure and chemical properties, ranging from small molecules with abundant reactive sites to act as active materials or copolymer reagents for many applications, to larger and more inactive molecules with higher thermal resistivity.

    Micromorphology control of the lignin-based activated carbon and the study on the pyrolysis and adsorption kinetics

    Liao, ZhouZhu, Ya-HongSun, Guo-TaoQiu, Ling...
    9页
    查看更多>>摘要:The waste corncob-based lignin (AL) treated by alkali was applied as feedstock to prepared activated carbon (AC) for the methylene blue (MB) adsorption. To explore the pyrolysis mechanism of ALs, the pyrolysis kinetics were discussed by the methods of Flynn-Wall-Ozawa (FWO) and Kissinger-Akahira-Sunose (KAS) equations at different heating rates, which are widely applied in the determination of dynamic parameters with reduction of the errors of multi-step dynamics. In the process of preparing lignin-based activated carbon (LC), the relationship between pyrolysis condition and structure of LCs was systematically investigated. The maximum specific surface area of LCs reached 1215.83 m2/g with high the mesoporous proportion (77.3%) at optimal activation conditions including activation temperature (550 degrees C), activation duration (120 min) and phosphoric acid concentration (50%). For the adsorption of methylene blue (MB) solution, the fitted kinetic model was in accordance with the pseudo-second-order kinetic model. The adsorption capacity of MB could achieve as high as 493.28 mg/g (+/- 5 mg/g), and the highest removal rate of MB could reach 99.0% (+/- 0.2%) during the secondary adsorption process. This work offers a prospective proposal to provide theoretical reference for producing LC based on pyrolysis of alkaline lignin, and utilize the LC as potential adsorbent in the dyeing wastewater treatment.