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Industrial Crops and Products
Elsevier Science Publishers
Industrial Crops and Products

Elsevier Science Publishers

0926-6690

Industrial Crops and Products/Journal Industrial Crops and ProductsSCIISTPEI
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    Characterization of the sugarcane MYC gene family and the negative regulatory role of ShMYC4 in response to pathogen stress

    Ren, YongjuanZou, WenhuiFeng, JingfangZhang, Chang...
    15页
    查看更多>>摘要:As a core regulator of the jasmonic acid (JA) signaling pathway, myelocytomatosis (MYC) transcription factors play a significant role in plant stress responses. However, there are currently no report on the MYC genes in sugarcane (Saccharum spp.), an important sugar and energy crop worldwide. Here, 23 SsMYC genes in Saccharum spontaneum, 3 ShMYC genes in Saccharum spp. hybrid R570 and 8 SbMYC genes in Sorghum bicolor were identified and classified into four groups (A-1, A-2, B, and C). Synteny analysis showed a close relationship among the MYC genes of S. spontaneum, R570, and S. bicolor. The cis-regulatory elements (CREs) in the promoter regions of the MYC genes were related to light, plant growth/development, hormones, and stress. RNA sequencing and realtime quantitative polymerase chain reaction analysis demonstrated that MYC genes were differentially expressed in sugarcane tissues and under Sporisorium scitamineum stress. A novel ShMYC4 gene was cloned from Saccharum spp. hybrid ROC22 and validated to be a nuclear-localized group A-1 member without transcriptional selfactivating activity. The decreased transcripts of ShMYC4 in six different sugarcane cultivars may have been associated with its negative response to S. scitamineum. Additionally, transient expression showed that ShMYC4 negatively regulated the defense response of Nicotiana benthamiana to Ralstonia solanacearum and Fusarium solani var. coeruleum by mediating the hypersensitive response, salicylic acid, JA and ethylene pathways. This study provided comprehensive information for the MYC gene family in sugarcane, which should be helpful for functional characterization of sugarcane MYC genes in the future.

    Agro-morphological and phytochemical studies of spearmint landraces (Mentha spicata L.) in Iran

    Mokhtarikhah, GhasemAyyari, MahdiEbadi, Mohammad-Taghi
    11页
    查看更多>>摘要:Spearmint (Mentha spicata L.) is one of the most important species of the Mentha genus, many of which are produced commercially on a large scale. Agro-morphological and phytochemical properties of 25 Iranian spearmint landraces were evaluated. The landraces were found to be significantly diverse phenotypically in terms of the following: plant height, main stem diameter, main stem length, number of lateral branches, number of leaves, length and width of fully grown leaves, length and width of inflorescence, fresh and dry weight, essential oil (EO) content and yield. The average dry weight yield, EO content, and yield of spearmint landraces were 520 g/m(2), 1.12% (v/w), and 5.8 mL/m(2), respectively. Overall, 40 constituents were recognized in the EOs, among which carvone (0.1-73.4%), menthone (0.1-62%), limonene (1.9-30.3%), neo-dihydro carveol (0.2-54.7%), pulegone (0.2-32.2%) and iso-menthone (0.03-26.3%) were the main components of all landraces, however, significant variation among them was detected. Among the tested landraces, Vardavard produced the highest content of EO, EO yield and indicated the highest carvone, respectively. Main stem length, fresh and dry weight yields significantly correlated with EO yield. Carvone showed a negative correlation with limonene. Morphological properties were not significant in terms of EO content and its constituents. In this research, it was obvious that the dendrogram differed more in morphological traits than phytochemical variables, which indi-cated the high potential of studied landraces for breeding programs.

    Improvement of xylo-oligosaccharides dissolution from Caragana korshinskii through liquid hot water pretreatment with tiny choline chloride

    Tian, ShenglongZhang, HuiFu, Shiyu
    9页
    查看更多>>摘要:Xylo-oligosaccharides (XOS) is a kind of high-value substance with bioactivity, extracted from plants with liquid hot water (LHW) treatment that is usually applied for biorefinery. Herein, we reported a system of LHW with tiny choline chloride (ChCl) to treat a shrub Caragana korshinskii to improve XOS extraction. It is found that the ChCl acted as a buffer in LHW pretreatment promotes the XOS extraction with a yield of 36.59% at 160 degrees C for 120 min. Compared with LHW/NaOH pretreatment, it also showed that the content of xylan in the residue was decreased by about 14% after LHW/ChCl pretreatment. The concentrations of furfural and 5-HMF in the hydrolysate were decreased after LHW/ChCl pretreatment. Moreover, the cellulose conversion rate of the residue treated with ChCl was 41.30% when using 25 FPU/g-substrate cellulase and 25 CBU/g-substrate beta-glucosidase, an increase of 38% compared with the untreated one. The LHW/ChCl pretreatment provided a strategy to utilize the sandy growth biomass for high value products.

    AMF and PGPR enhance yield and secondary metabolite profile of basil (Ocimum basilicum L.)

    Karik, UnalYilmaz, Abdurrahim
    8页
    查看更多>>摘要:Basil (Ocimum basilicum L.), one of the valuable fresh vegetables and medicinal plants, is widely cultivated in warm areas of Africa, Asia, and the Mediterranean. The essential oil of basil has spacious use in fragrances, food flavor, and the cosmetics sector. This study aimed to determine the effects of AMF (Arbuscular Mycorrhizal Fungi), PGPR (Plant Growth-Promoting Rhizobacteria), and their combination on yield and essential oil composition of basil in Bolu (Turkey) ecological conditions. In the research, plant height, fresh herbal yield, fresh leaf yield, dry herbal yield, dry leaf yield, and essential oil percentage and content were determined. Relationships among the properties and applications were evaluated by multivariate analysis. Microorganism applications yielded statistically superior values compared to the control in all properties. The AMF application granted higher essential oil yield and percentage (0.77%, and 1.23-ml plant(-1) respectively) than the other applications. Eugenol and gamma-cadinene, which are two of the highest ratio components of the essential oil composition, were higher (7.22% and 5.06%, respectively) in the AMF. Correlation analysis revealed the importance of germacrene-D and camphor on the terpenoid synthesis pathway. The results proved that AMF increases not only the yield properties but also some of healthy secondary metabolites.

    Characterization of the sugarcane MYC gene family and the negative regulatory role of ShMYC4 in response to pathogen stress (vol 176, 114292, 2022)

    Su, WeihuaZhao, ZhennanWang, DongjiaoSun, Tingting...
    2页

    Transcriptomic and physiological analyses of Miscanthus lutarioriparius in response to plumbum stress

    Wang, JiaDuan, XuchuWang, YaozhouSheng, Jiajing...
    9页
    查看更多>>摘要:Soil plumbum (Pb) contamination has become a global environmental issue, which threaten soil quality, food security and human health. Miscanthus species have exhibited high tolerance and restoration capacity to heavy metals stress. However, the mechanisms of Pb resistance in Miscanthus species have not been fully elucidated. In the current report, transcriptome changes of Miscanthus lutarioriparius response to different Pb treatment time were analyzed to investigate the key genes and pathways involved in Pb uptake patterns. Based on the assembled transcriptome data, totally 19332 differentially expressed genes (DEGs), in which 200-11313 DEGs in each comparison group were identified respectively. Gene ontology and pathway enrichment analysis found that different expression genes under Pb stress are predominantly involved in the pathways of phenylpropanoid biosynthesis and metabolism, biosynthesis of secondary metabolites, photosynthesis and metal ion transporters. The results in the current study could serve as a ground work for future studies to determine the exact expression of genes in plants under heavy metal stress, the processes involved and how they can be efficiently utilized to improve plant tolerance to toxic metals and facilitate the remediation process.

    Effect of gamma radiation on gentiopicroside production in Gentiana kurroo Royle in vitro cultures

    Thiagarajan, KalaivaniFulzele, Devanand P.Pillay, MichaelSatdive, Ramesh K....
    8页
    查看更多>>摘要:Gentiopicroside (GPD) is one of the vital bioactive molecules present in the rhizomes, roots and aerial parts of Gentiana kurroo Royle. In this study, callus and in vitro shoots cultures of G. kurroo were treated with different doses of gamma radiation to assess its effect on the concentration of GPD. The callus was established on MS media fortified with Indole-3-butyric acid (IBA) and Indole-3-acetic acid (IAA) and exposed to variable radiation doses (0, 10, 20, 30, 40, 50, or 60 Gy Co-60 gamma rays). Similarly, in vitro shoots raised in MS media supple-mented with 6-Benzylaminopurine (BAP) and IAA were irradiated at (0, 10, 20, 30, 40, 50, 60, or 70 Gy Co-60 gamma rays). In vitro cell growth and GPD content were assessed during M1V1, M1V2, M1V3, and M1V4 (Mutation and Vegetative) generations. The lethal dose (LD50) for both callus and shoots were observed at 35 Gy. The results revealed that low radiation doses favored growth and showed increased GPD content in the callus cultures during M1V1 generation, but both parameters were reduced in the M1V2, M1V3 and M1V4 generations. However, shoots irradiated at low doses boosted the growth and GPD level during M1V2, M1V3 and M1V4 generations. This is the first report that used gamma radiation to show the effect of GPD elicitation in the genus Gentiana.

    Biodegradable starch / polyvinyl alcohol composites produced by thermoplastic injection containing cellulose extracted from soybean hulls (Glycine max L.)

    Bortolatto, RubianeStival Bittencourt, Paulo RodrigoYamashita, Fabio
    11页
    查看更多>>摘要:Soybean hull is an agro-industrial residue of extensive generation in the soybean oil-producing industry, where approximately 5% of the processed volume of soy beans is converted into hulls. The main use for soy hulls is animal feed, being of great importance to find more ways of consumption for the hulls. This research proposed a noble application for soy hulls: using the hulls as a source for extracting cellulose fibers. Cellulose fibers were extracted from the hulls by alkaline treatment, ground (< 600 mu m) and added to thermoplastic starch (TPS). Polyvinyl alcohol (PVA) was also added to the materials produced in order to promote better compatibility between the components. Ten formulations were produced, containing between 4 and 19 wt% of cellulose, and 2 controls. Half of the formulations contained 6 wt% PVA. Cellulose fibers and soybean hulls were characterized by FTIR-ATR and SEM, and the analyzes showed partial purification of the cellulose fibers, with a purity of 92 wt%. The composites were produced by extrusion followed by injection, and characterized by stress-strain, FTIR-ATR and dimensional stability after injection (shrinkage and warpage) tests. Cellulose fibers did not increase the tensile strength (TS) of the composites, but reduced the deformation capacity by 62% (to 19% of fibers) compared to the control. Cellulose fibers together with PVA increased the TS by up to 22% (to 19% fibers and 6% PVA in the composition), making the material more mechanically resistant. For this same formulation, the deformation capacity was reduced by a maximum of 75%, and the modulus of elasticity was increased by a maximum of 230% (the composite became more resistant and rigid). Cellulose fibers reduced the shrinkage value of the specimens, reducing the shrinkage value to less than 1% for the formulations containing 19% cellulose fibers. The cellulose fibers associated with 6% PVA had a synergistic action in the shrinkage of the specimens, further reducing the shrinkage in length (0.4% length shrinkage for 15% cellulose fibers; 0.1% length shrinkage for 19% cellulose fibers). The addition of cellulose fibers and 6% PVA to TPS made the composites more rigid and mechanically reinforced, and also with greater dimensional stability after injection.

    Changes of lignin structure of poplar wood chips in autohydrolysis pretreatment and bleachability of chemi-thermomechanical pulp

    Chen, YunjingYu, ShiyunHou, QingxiWu, Ming...
    9页
    查看更多>>摘要:In this work, poplar wood chips (PWCs) were subjected to autohydrolysis pretreatment with different levels of intensity, and the autohydrolyzed PWCs were used to make chemi-thermomechanical pulp (CTMP). The changes of the lignin structure of PWCs and the bleachability of the CTMP with autohydrolyzed PWCs as raw materials were both investigated. The results showed that the lignin structure changed to some extent in both CTMP pulping and the following bleaching process. The contents of conjugated carbonyl and quinone groups in the lignin structure were negatively correlated with the CTMP bleachability. Compared to the quinone groups, the conjugated carbonyl groups had a much greater impact on the bleachability. The present work would play a vital role for the application of autohydrolysis to the poplar CTMP pulping and the subsequent bleaching processes.

    Light-colored lignin isolated from poplar by ultrasound-assisted ethanol extraction: Structural features and anti-ultraviolet and anti-oxidation activities

    Su, XiuruFu, YingjuanShao, ZhiyongQin, Menghua...
    9页
    查看更多>>摘要:Ethanol extraction of lignocellulosic biomass under mild conditions has re-attracted considerable attention since a light-colored lignin with low molecular weight can be obtained. Unfortunately, the yield of isolated lignin for the ethanol extraction process is very limited. To enhance the extraction efficiency of lignin from poplar, ultrasound was used to assist the ethanol extraction and the isolated lignin was analyzed in terms of structure features and functional properties. As expected, increasing the ultrasonic power and ultrasonic time led to higher lignin yields during the ultrasound-assisted ethanol extraction (UAEE) process. The chemical structure of the isolated lignin (UAE-L) was almost completely retained, the C-O (mainly beta-O-4) linkages in the lignin only exhibited a slight decrease, while the C-C (beta-beta, beta-5, and beta-1) linkages were nearly unchanged. The UAE-L was light in color and had more phenolic hydroxyl groups, less carbohydrate impurities, lower molecular weight and narrower polydispersity as compared to the poplar MWL. The UAE-L could scavenge more 2,2-diphenyl-1-picryl-hydrazyl radicals (DPPH center dot), displaying a stronger antioxidant activity. Moreover, the UAE-L showed good UV-absorbing properties in both UVA and UVB regions. These results indicated that the UAE-L has great potential for replacing the commercial synthesized antioxidant and anti-UV ingredients.