首页期刊导航|Industrial Crops and Products
期刊信息/Journal information
Industrial Crops and Products
Elsevier Science Publishers
Industrial Crops and Products

Elsevier Science Publishers

0926-6690

Industrial Crops and Products/Journal Industrial Crops and ProductsSCIISTPEI
正式出版
收录年代

    Isolation and characterization of a novel xanthone from the hairy root cultures of Swertia chirayita (Roxb.) H. Karst. and its biological activity

    Shanker, KarunaRahman, Laiq UrMahendran, GanesanVerma, Narsingh...
    11页
    查看更多>>摘要：Swertia chirayita (Roxb.) H. Karst. is a well-documented traditional plant used to cure liver diseases, malaria fever, jaundice, hepatitis and diabetes. Xanthones are produced by the plant and provide a variety of health benefits. The goal of this research is to develop an efficient and reliable approach for hairy root induction and to explore the biosynthesis of xanthones in hairy root lines. Infection of S. chirayita leaves with Rhizobium rhizogenes strain (LBA 9402) resulted in distinct hairy root lines. The rolB gene integration in the root was validated by polymerase chain reaction (PCR) in four hairy root lines. Hairy root line SA produced the maximum biomass of 13.32 +/- 0.50 g/flask FW (fresh weight) and 1.29 +/- 0.05 g/flask DW (dry weight) after 60 days. A novel 1,2,5,6tetrahydroxyxanthone (2) was isolated and characterized from SA hairy root line using flash chromatography. Swerchirin (1) (0.382 +/- 0.089 mg/g DW) and 1,2,5,6-tetrahydroxyxanthone (2) (0.935 +/- 0.02 mg/g DW) along with swertianin (0.0195 +/- 0.007 mg/g DW) were accumulated higher amount in SA line. 1,2,5,6-tetrahydroxyxanthone (2) exhibited strong antioxidant activity in DPPH (IC50 = 1.60 +/- 0.004 mu g/mL) and FRAP (4.83 +/- 0.02 Eq. to FeSO4) assay. Further, swerchirin (1) exhibited anti-proliferative activity by suppressing the growth of MDA-MB-231 cell line with IC50 of 18.82 mu g/mL (65.83% at 50 mu g/mL) and 1,2,5,6-tetrahydroxyxanthone (2) inhibited the growth of K562 cell line with IC50 of 22.83 mu g/mL (67.82% at 50 mu g/mL). The characterized compounds with promising biological activity could be taken up for its commercial production.
      原文链接:
    • NSTL
    • Elsevier

    Meta-analysis of QTLs controlling seed quality traits based on QTL alignment in Brassica napus

    Bilgrami, SayedehsabaLiu, LiezhaoFarokhzadeh, SaraNajafabadi, Ahmad Sobhani...
    15页
    查看更多>>摘要：A growing demand in oilseed use for food, feed and fuel has caused added pressure on rapeseed breeders to improve higher-quality cultivars. To gain a better understanding of the genetics of oil, fatty acids, sterols, protein and amino acids contents in Brassica napus, 2138 QTLs in 16 populations were collected from 22 studies published since 2000. The physical position of 1567 initial QTLs could be found in the physical map of Darmor-bzh that finally revealed 294 QTL overlapping regions from different populations potentially associated with seed quality traits, distributed unequally in most of the chromosomes. We detected enrichment regions with more than 10 initial QTLs in some traits, which could provide clues in discovering varied or protected areas. Additionally, 111 overlapping QTLs regions were found, controlling at least two traits. These regions with broad pleiotropic effects or tight linkages might have merit in improving B. napus in targeting more than one desirable trait simultaneously. Furthermore, to validate our findings, candidate genes were subjected to three types of gene set analyses: KEGG pathway and interaction networks for Arabidopsis thaliana genes, and expression enrichment analysis for candidate genes of B. napus. The results indicated key known genes, novel genes, and genes with unknown functions in the overlapping regions that were involved in different biological processes of quality traits, such as highly expressed genes in seed, including BnaA08g13680D, BnaA08g13750D, BnaA08g13730D, BnaA08g14030D, BnaA08g14060D, BnaA08g12340D and BnaC05g42910D, which were observed in overlapping QTLs of multiple traits. Further validation also depicted that 107 overlapping QTLs were co-located with 366 SNP peak positions reported based on the genome-wide association studies (GWAS) for quality traits in rapeseed. Our results will serve as a resource for future researches in functional gene analysis, genetic engineering and breeding programs.
      原文链接:
    • NSTL
    • Elsevier

    De novo transcriptome analysis of bamboo in vitro shoots for identification of genes differentiating juvenile and aged plants

    Thapa, PoojaSareen, BhuvneshSwarnkar, Mohit KumarSood, Anil...
    15页
    查看更多>>摘要：The industrially important bamboo, Dendrocalamus hamiltonii Nees et Arn. ex Munro is monocarpic and flowers gregariously only once after 40-60 years. The age of most of the plants growing in the wild is not known. This makes it impossible to distinguish between the juvenile and aged plants that are about-to-flower and die. Conservation of juvenile plants, but utilization of aged ones is therefore, extremely difficult. Since identification of genes specific to about-to-flower plants was envisaged to facilitate this differentiation between juvenile and aged plants, de novo transcriptome analysis of vegetative, about-to-flower and flowering in vitro shoots of D. hamiltonii was undertaken. A total of 195 differentially-expressed-genes were identified. Key genes specific to about-to-flower stage (AFS) were identified and validated by q-PCR in plants growing in wild. The q-PCR expression patterns of selected genes including the key regulators of floral transition i.e., CONSTANS-like (COL), phytochrome interacting factor 5 (PIF5), VERNALIZATION INSENSITIVE 3 (VIN3), flowering time control protein (FCA), transcription factor GAMYB (GAMYB), squamosa promoter-binding-like protein 3 (SPL3), heading date 3a (Hd3a), apetala 2 (AP2) and MADS box genes matched exactly in the about-to-flower shoots (AFS) of in vitro and sporadic about to flower shoots (SAFS) of bamboos growing in the wild. These can serve as markers of 'floral transition'. Establishment of a close homology of D. hamiltonii with D. latiflorus Munro, Phyllostachys edulis (Carriere) J. Houzeau, Bambusa oldhamii Munro and B. multiplex (Lour.) Raeusch. ex Schult. through multiple sequence alignment and phylogenetic analysis of PIF5, VIN3, SPL3, and MADS-box transcription factor 15 (MADS15) suggested the potential applicability of identified genes in other industrially-important-bamboos. Transcriptional corepressor LEUNIG (LUG), transcription regulatory protein SWI3D (SW13D), protein HOTHEAD (HTH) and E3 ubiquitin ligase BIG BROTHER-related (BBR) were identified in D. hamiltonii for the first time. The q-PCR analysis of identified genes can serve as potential tool for distinguishing juvenile and about-to flower aged bamboo plants growing in the wild. The study is a step towards the conservation of juvenile bamboos as a sustainable resource base for future industrial applications.
      原文链接:
    • NSTL
    • Elsevier

    RNA-seq analysis revealed key genes associated with salt tolerance in rapeseed germination through carbohydrate metabolism, hormone, and MAPK signaling pathways

    Mohamed, Ibrahim A. A.Shalby, NesmaEl-Badri, Ali MahmoudBatool, Maria...
    18页
    查看更多>>摘要：Seedling survival, growth, and yield are highly associated with seed germination performance. Germination of rapeseed differs under NaCl stress among cultivars, but the molecular mechanism of salinity tolerance in rapeseed is poorly understood. In the current study, in contrast to the trend of the hydrogen peroxide level, the activities of superoxide dismutase, catalase, and ascorbate peroxidase were increased in the salt-tolerant (Yangyou9; YY9) cultivar as compared with the salt-sensitive one (Zhongshuang11; ZS11). Integrative transcriptome analysis of four seed germination phases was carried out on the two rapeseed cultivars, which showed a substantial transcriptional change at 24 and 48 h. Different pairwise comparisons were performed to provide a comprehensive view of the biochemical processes that underpin salt tolerance and seed germination. Plant hormone signal transduction, MAPK signaling, glycolysis/gluconeogenesis, fermentation, and tricarboxylic cycle pathways were significantly enriched. Genes related to auxin (Aux) signaling, i.e., BnaAUX1, BnaTIR1, BnaGH3.5/17, and BnaSAUR were associated with salt tolerance through activating the germination process. Genes related to cytokinin (Ck; BnaAHP1/3/4 and BnaA-ARR3/4/8/9), gibberellin (GA; BnaPIF3/4/5), brassinosteroids (BRs; BnaBAK1, BnaBSK, BnaBZR1/2, BnaTCH4, and BnaCYCD3), and salicylic acid (SA; BnaNPR1/3/ 5, BnaPR1, and BnaTGA2/4) signaling transductions were linked to salt tolerance at the germination stage. While genes related to abscisic acid (ABA; BnaPP2C and BnaABF) and ethylene (Eth; BnaEIN3 and BnaERF1/2) were relatively upregulated in the dry seeds and salt-stressed sensitive cultivar, therefore, these genes may participate in salt sensitivity and seed dormancy. Moreover, the enzymatic activities (fructose-bisphosphate aldolase and isocitrate dehydrogenase) and content of phytohormones (indole-3-acetic acid (IAA), Ck, and GA) were significantly (p < 0.05) higher in YY9 than in ZS11, while ABA content showed a reverse trend, indicating the reliability of the RNA-seq data. Overall, this is the first study that provides new insights about transcripts involved in the salt tolerance during seed germination of rapeseed.
      原文链接:
    • NSTL
    • Elsevier

    Phytochemical fidelity and therapeutic activity of micropropagated Curcuma amada Roxb.: A valuable medicinal herb

    Behera, ShashikantaMonalisa, KumariMeher, Rajesh K.Mohapatra, Soumyajit...
    11页
    查看更多>>摘要：Curcuma amada Roxb. is a valuable medicinal herb with food, economic, pharmaceutical, and industrial value. This plant is commonly propagated through rhizome which is season dependent and slow process. Moreover, rhizomes are easily infected by different soil-borne pathogen, which deteriorates the quality and quantity of production. Hence, this study was carried out to develop one step plant regeneration protocol using rhizomatic bud on meta-Topolin (mT) supplemented medium. About 600 plants were produced on Murashige and Skoog (1962) (MS) medium added with 3.0 mg/L mT after twelve weeks of culture. Well rooted plants were successfully hardened (95%) and subsequently all the plants were established in the field. Quantitative phytochemical analysis and assessment of chemical composition of essential oil by Gas Chromatography - Mass Spectroscopy (GC-MS) confirmed the biochemical fidelity of the field-established tissue culture plants. Robust therapeutic activities such as antioxidant, anticancer, and antimicrobial activities were found in the rhizome extract and essential oil of the field-established tissue culture plants. It was concluded that tissue cultured plants are at par with the mother plant in terms of phytochemicals composition and therapeutic activity. Thus, this protocol could be useful for the production of a large number of plants aiming for its conservation, propagation as well as sustainable utilization in food, beverage, and pharmaceutical industries.
      原文链接:
    • NSTL
    • Elsevier

    Application of UF adhesives containing unmodified and phosphate-modified cellulose microfibers in the manufacturing of particleboard composites

    Benhamou, Anass AitBoussetta, AbdelghaniKassab, ZinebNadifiyine, Mehdi...
    15页
    查看更多>>摘要：In recent years, the application of cellulose derivative materials as reinforcement in wood adhesives has attracted considerable attention. This study aimed to explore the characteristics of cellulose microfibers (CMF) and phosphorylated cellulose microfibers (P-CMF) successfully extracted from giant reed plant, and their potential effect as binders in the formulation of particleboard panels-based urea-formaldehyde (UF) adhesive. The influence of fiber content (5%, 7.5%, and 10%) on the mechanical and physical properties of the prepared composites were assessed. Moreover, the fire-retardant behaviour of panels containing the optimal amount of phosphate was evaluated to study phosphate groups' efficiency in improving the resistance character of the panels towards the fire. Results showed that the mechanical properties were improved by the addition of 7.5% of CMF and P-CMF. The physical and fire-retardant properties were found to be significantly enhanced for panels containing 7.5% of P-CMF as a result of the chemical phosphorylation.
      原文链接:
    • NSTL
    • Elsevier

    Comparative metabolomics applied for valorization of Mesquite (Prosopis juliflora (Sw. DC.) by-products as potential source of anti-inflammatory functional constituents

    Yassin, Abdelrahman M.Ghareeb, Doaa A.Harraz, Fathallah M.Shawky, Eman...
    11页
    查看更多>>摘要：Mesquite (Prosopis juliflora (Sw.) DC.) is widely used in food, biotechnology, pharmaceutical, and cosmetic industries. Every part of mesquite is used for medicinal purposes. In this work, stems, leaves, flowers and pods of P. juliflora were chemically profiled via UPLC/MS/MS analysis for their valorization as forest by-products with tentative identification of 44 metabolites. Multivariate statistical analysis was implemented to investigate inbetween and within class discrimination of the different parts of mesquite where stems and leaves extracts were separately segregated indicating great variation in their chemical profile, while pods and flowers were clustered together. Ex-vivo anti-inflammatory activity of the tested extracts on four pro-inflammatory markers (TNF-alpha, IL-1 beta, IL-6 and INF-gamma) were performed where stems extracts showed significant inhibition to the tested cytokines. Orthogonal projection to latent structures (OPLS) models and coefficient plots of each proinflammatory marker unraveled the important functional constituents that positively correlated to inhibitory activity of each cytokine. 3 '-oxojuliprosine, isojuliprosine, and palmitic acid have high coefficient correlation values regarding inhibiting TNF-alpha and IL-1 beta pro-inflammatory markers, where they showed spatial correlation to flowers extracts. Meanwhile, stems and leaves were spatially correlated to downregulation of IL-6 and INF-gamma levels with retusin-7-neohesperidoside, tryptamine and schaftoside, being the most significant coefficients. Results indicate the possible use of different parts of mesquite a potential source of functional components as highvalue-added byproducts.
      原文链接:
    • NSTL
    • Elsevier

    Preparation and antibacterial properties of hemp cellulose-based material based on Schiff base between lysine grafted N-halamine and dialdehyde hemp

    Wang, RuiqiangXue, HongyingLeng, JinpengZhang, Jialiang...
    7页
    查看更多>>摘要：Bacteria have exerted a great threat to the human living environment. Therefore, many industries put forward higher requirements for the antibacterial properties of cellulose-based materials, especially the applications in food packaging, biomedical, and textile industry. In this paper, through using synthetic lysine grafted N-halamine organic antibacterial agent and hemp fiber as raw materials, the lysine grafted N-halamine Schiff base type hemp cellulose-based antibacterial material is prepared by simple and mild Schiff base reaction. This hemp cellulose-based antibacterial material combines various sterilization methods of hemp fiber, amino acid type Schiff base and N-halamine. The synergistic cooperation of different sterilization methods greatly improves the antibacterial performance of material and reduces the possible bacterial resistance. The antibacterial examination shows that S. aureus and E. coli could be killed totally by this material in 5 min, and the inhibition zone is increased to 18.4 mm. Meanwhile, this hemp cellulose-based antibacterial material has low cost, green preparation, excellent storage stability and regeneration ability, which provides a novel way for the development of synergistic application of different antibacterial agents and the use of cellulose-based materials in bacterial environment.
      原文链接:
    • NSTL
    • Elsevier

    Application of deep eutectic solvent as novel co-solvent for oil extraction from flaxseed using sonoenergy

    Hayyan, AdeebSamyudia, Adrian, VHashim, Mohd AliHizaddin, Hanee F....
    9页
    查看更多>>摘要：The extraction of oil from flaxseeds were carried out using deep eutectic solvents (DESs) and organic solvents with ultrasound assisted extraction. Enhancement of solvent extraction was investigated by adding novel cosolvents in the form of DESs to tetrahydrofuran (THF) and n-hexane. COSMO-RS, UFF and DFT computational method was used to study the solubility of organic solvents and DESs for oil extraction from flaxseed. It was noted that the solvent share the same non-polar region on the sigma-profiles with the flaxseed oil model. Different organic solvents were screened and it was found that n-hexane and THF can achieve the highest oil yield. The addition of DES to THF negatively impacted oil yield. On the other hand, the addition of DES to n-hexane can slightly improve the extraction yield and significantly reduce the extraction temperature in comparison to nhexane alone. Application of DES as novel co-solvent with other organic solvents can be translated to economic benefits through reducing the energy consumption and operational risks associated with the use of flammable solvent such as n-hexane.
      原文链接:
    • NSTL
    • Elsevier

    Acidolysis mechanism of lignin from bagasse during p-toluenesulfonic acid treatment

    Feng, ChengqiZhu, JiatianCao, LimingYan, Li...
    11页
    查看更多>>摘要：Lignin was efficiently separated from lignocellulosic biomass using p-toluenesulfonic acid (p-TsOH) treatment. However, lignin depolymerization and acidolysis have not been studied to date. In this study, the mechanism of lignin acidolysis was investigated. The structure of lignin in bagasse before and after the p-TsOH treatment was analyzed. The efficiency and selectivity of lignin depolymerization were evaluated. The lignin removal rate reached 88.81%. Hemicellulose was completely removed from lignin, and cellulose destruction was inhibited. The structure of lignin in bagasse was analyzed using nuclear magnetic resonance (NMR). The results revealed that alpha-methoxylated beta-O-4 intermediates were formed during lignin depolymerization. This indicated that the benzyl ether bonds were effectively catalyzed and broke, thereby promoting lignin dissolution. The lignin-carbohydrate complex structure was destroyed. The insoluble and soluble lignin in the hydrolysate were analyzed using NMR and gas chromatography-mass spectrometry. The results indicated that the catalyst promoted breaking of the C beta-O bonds in the beta-O-4 structures. Phenols and Hibbert ketones were formed. In addition, lignin sulfonation was confirmed by the presence of di-o-tolusulfone and di-p-tolusulfone. The low contents of beta-beta and beta-5 bonds in the reaction products indicated that lignin condensation was inhibited. The results demonstrated that lignin acidolysis was synergistically catalyzed by H+ and p-TsOH. It provides important theoretical support for efficient and clean separation of lignin using the p-TsOH treatment.
      原文链接:
    • NSTL
    • Elsevier