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Industrial Crops and Products
Elsevier Science Publishers
Industrial Crops and Products

Elsevier Science Publishers

0926-6690

Industrial Crops and Products/Journal Industrial Crops and ProductsSCIISTPEI
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    Phytohormone and transcriptome of pericarp reveals jasmonate and LcMYC2 are involved in neral and geranial biosynthesis in Litsea cubeba

    Wang M.Zhao Y.Gao M.Wu L....
    10页
    查看更多>>摘要：? 2021 Elsevier B.V.Litsea cubeba (Lour.) Person (Lauraceae) is a famous essential oil tree that produces L. cubeba essential oil (LCEO) in the pericarp. LCEO, mainly containing neral and geranial (~80%), has been widely used in medical treatment, cosmetics, food and other fields. Knowledge of the development of the L. cubeba pericarp would provide an important basis for LCEO production and genetic breeding. Here, according to the characteristics of the pericarp development process, we provide the phytohormone profiles, including auxin, gibberellin (GA), abscisic acid (ABA), cytokinin (CK), and jasmonate (JA), at 8 timepoints covering the development process of fruit growth, maturation and fruit ripening periods. The results indicated that only the dynamics of JA were consistent with the increasing trend of LCEO, neral, and geranial. Meanwhile, RNA-seq was used to investigate the expression of the genes, especially the genes involved in signal transduction pathways of phytohormones, during L. cubeba pericarp development. The crosstalk among these phytohormone-responsive genes indicated that the hub transcription factor LcMYC2 was also involved in the JA signalling pathway. Furthermore, treatment with MeJA and transient overexpression of LcMYC2 both resulted in significant accumulation of neral and geranial in L. cubeba. Finally, LcMYC2 was verified to promote neral and geranial biosynthesis by transient expression assay, and LcMYC2 enhanced the transcription of LcTPS42 and LcGPPS.SSU1 promoters are key enzymes responsible for the biosynthesis of the above component by yeast one-hybrid (Y1H) and dual-luciferase (dual-LUC) assays. Our data not only provided the profiles of phytohormones and the transcriptome during the development process of the pericarp but also identified the important roles of JA and the hub regulator LcMYC2 in the accumulation of LCEO in the L. cubeba pericarp.
      原文链接:
    • NSTL
    • Elsevier

    Effect of Olive Mill Wastewater on the composition of the essential oil of bergamot-mint under semi-arid climate

    El Hassani F.Z.Aissam H.Merzouki M.Benlemlih M....
    7页
    查看更多>>摘要：? 2021 Elsevier B.V.In this work, we investigate the changes in Mentha aquatica var. citrata (bergamot-mint) characteristics and essential oil composition regarding the modification of the amended dose of Olive Mill Wastewater (OMW) in pots at three different doses; D1 = 9 ml/100 g, D2 = 22.5 ml/100 g and D3 = 54 ml/100 g of soil, seven months before plantation of bergamot-mint cuttings. OMW application didn't affect plant biomass production (leaves, stems, roots) and chlorophylls in the leaf, while the amount of leaf phenols showed a dose dependent increase. Bergamot-mint essential oil major compound is linalool acetate for control, trans-pulegol when OMW is applied at 9 ml/100 g, isopulegyl acetate when OMW is applied at 22.5 ml/100 g, and verbenone when OMW is applied at 54 ml/100 g. When OMW was applied to soil at doses D1, D2 and D3, some of bergamot-mint essential oil compounds such as decanone, citronellol, piperitone epoxide, guaiacol acetate, methyl cinnamate and citronellyl propanoate, totally disappeared. OMW spreading at 9 ml/100 g allowed appearance of menthone in Mentha aquatica var. citrata essential oil while menthol and terpineol appeared only when OMW was applied at the high dose 54 ml/100 g.
      原文链接:
    • NSTL
    • Elsevier

    Acaricidal, ovicidal and fagoinhibition activities of seed extracts from Swietenia humilis against Tetranychus urticae under laboratory conditions

    Peraza-Campos A.L.Parra-Delgado H.Chan-Cupul W.Maldonado-Michel M.A....
    8页
    查看更多>>摘要：? 2022Two-spotted spider mite (Tetranychus urticae Koch) has been considered among the most important phytophagous pest both in field and greenhouse crops around tropical areas of the globe. Although synthetic pesticides have remained as the main method to control mite populations, alternatives strategies as biopesticides based on secondary metabolites derived from plants should be considered. Ascending polarity seed extracts of Swietenia humilis Zucc. (Meliaceae: Magnoliopsida) were evaluated for their egg and adult female toxicity, fagoinhibition as well as repellent activities against T. urticae under laboratory conditions. Ethyl acetate (EtOAc) medium polarity extract showed the highest ovicidal activity (92.54% at 1% concentration), adult female corrected mortality (EC50 = 0.0738%) as well as fagoinhibition (100% at 0.25% concentration). On the other hand, high polarity Methanol (MeOH) extract exhibits a predominant repellent profile (62% repellency) that was observed at 0.125% concentration with no significant toxicity. Our findings suggest different activity of extracts depending on their polarity. Otherwise, 13C NMR of a fraction from EtOAc extract led to detection of two major mexicanolide-type limonoids named humilinolides G and H. Finally, this study provides important bases for further research on the value of S. humilis seed extracts (ShSE) as an acaricide and the comprehensive use of the seeds for industrial multipurpose.
      原文链接:
    • NSTL
    • Elsevier

    Enzyme-deep eutectic solvent pre-treatment for extraction of essential oil from Mentha haplocalyx Briq. leaves: Kinetic, chemical composition and inhibitory enzyme activity

    Li Z.Wang H.Pan X.Guo Y....
    11页
    查看更多>>摘要：? 2021 Elsevier B.V.In this study, an improved method, enzyme-deep eutectic solvent pre-treatment followed by microwave-assisted hydrodistillation (EDP-MAHD), was applied to extract essential oil from the leaves of M. haplocalyx Briq. The synergistic effects of enzymatic hydrolysis, deep eutectic solvent, and microwave irradiation on the extraction kinetics and composition of essential oil were systematically evaluated. The yield of essential oil was improved under the pre-treatment conditions of mixed enzymes (cellulase and pectinase of 1:1) and choline chloride/glucose. By investigating the pre-treatment and microwave-assisted hydrodistillation processes, the yield of essential oil was enhanced to 2.19%. The results of GC-MS showed that menthol was the most important component in essential oil, with the content of 62.43%, and other components including menthone, piperitone, isomenthone, germacrene D, etc. Compared with other methods, EDP-MAHD could produce a higher yield of essential oil and make the essential oil have higher inhibitory activity on acetylcholinesterase and α-amylase (IC50 value of 6.36 and 34.32 mg/mL, respectively).
      原文链接:
    • NSTL
    • Elsevier

    Co-modification of corn straw lignin and its enhancement on glue-free fiberboard based on freezing activated wood fibers

    Zhang K.Liu Y.Guo Z.Wang J....
    8页
    查看更多>>摘要：? 2021 Elsevier B.V.Glue-free fiberboard, as a substitute for traditional fiberboard, does not contain a large amount of synthetic resins, which can avoid harm to human health and environment. However, glue-free fiberboard still has some disadvantages of insufficient mechanical strength and excessive thickness swelling of water absorption. Here, corn straw lignin was co-modified by demethylation/hydrothermal degradation to obtain high hydroxyl content increased by 210.85% compared with raw lignin. The co-modified lignin was then introduced into freezing activated wood fibers to prepare the environment-friendly medium density fiberboard (MDF). The effect of co-modified lignin content on the properties of MDF was investigated. MDF with 3 wt% co-modified lignin exhibited the best comprehensive performance with the modulus of elasticity (MOE) of 5809 MPa, modulus of rupture (MOR) as high as 70 MPa, internal bonding strength (IB) of 2.29 MPa and thickness swelling (TS) as low as 16.65%, completely matching the requirements of the Chinese national standard GB/T 11718-2009 and EU national standard BS EN 622–5–2009. The interaction mechanism of co-modified lignin on the wood fibers was further explored by Fourier infrared spectroscopy (FTIR), differential scanning calorimeter (DSC) and scanning electron microscope (SEM) analysis. The phenolic structures of co-modified lignin could be chemically crosslinked with wood fibers during the hot-pressing process and the large amount of hydroxyl groups provided strong hydrogen bond interactions for the glue-free fiberboard. The glue-free fiberboard enhanced by co-modified lignin with excellent comprehensive performance exhibits a broad application prospect.1
      原文链接:
    • NSTL
    • Elsevier

    D-Lactic acid production from Cistus ladanifer residues: Co-fermentation of pentoses and hexoses by Escherichia coli JU15

    Alves-Ferreira J.Carvalheiro F.Duarte L.C.Ferreira A.R.P....
    12页
    查看更多>>摘要：? 2022 Elsevier B.V.In this study, glucan-rich solids, and xylose-rich hydrolysates obtained from Cistus ladanifer distillery residues (CLR) were used for D-lactic acid (D-LA) production by the D-lactogenic Escherichia coli strain JU15. Firstly, hemicellulosic hydrolysates obtained by the autohydrolysis process were submitted to dilute sulfuric acid-catalysed post-hydrolysis. The influence of operational conditions on oligosaccharides hydrolysis was assessed by the combined severity parameter (CS) in the range of 1.1–2.3. The optimum post-hydrolysis conditions were found for CS of 1.6 (300 mM H2SO4, 15 min, 121 °C). Subsequent detoxification procedures on post hydrolysed liquors were carried out, where 9.1% (w/v) powdered activated charcoal enabled a full removal of furfural, 5-hydroxymethylfurfural (HMF), and phenolic compounds together with a reduction of acetic acid (37%), and formic acid (27%). Diverse fermentation modes using detoxified and non-detoxified hydrolysates, as well as using previously NaOH delignified glucan-rich solids alone (SHF or SSF) or together with pentoses liquors (SSCF) (5% loading) were performed. For all the tested conditions, both hemicellulose- and cellulose-derived sugars can be efficiently used as the carbon source to produce D-lactic acid by E. coli JU15 with a D-LA yield always surpassing 92 gd-LA/100 g sugars.
      原文链接:
    • NSTL
    • Elsevier

    Agro-industrial byproducts as modification enhancers of the bacterial cellulose biofilm surface properties: An inverse chromatography approach

    Faria M.Aguiar R.Cordeiro N.Mohammadkazemi F....
    9页
    查看更多>>摘要：? 2021 Elsevier B.V.Bacterial cellulose (BC) has remarkable and excellent properties. However, the high-cost production and the use of chemicals for BC modification make its application unattractive. A simple approach to modify the properties of BC during biosynthesis, by using alternative low-cost carbon sources from agro-industrial byproducts, was studied. The carbon source (date syrup, mannitol, sucrose, food-grade sucrose, and glucose) significantly changed the BC network morphology and structure. Date syrup source induces the highest modifications in the surface properties of BC: smaller area (SBET = 4.04 m2/g), higher hydrophobic (γsd = 45.79 mJ/ m2) and basic character (Kb/Ka = 1.10), at 25 oC. Food-grade sucrose source resulted in the lowest yield of BC production (37% less), however caused an increase in the BC network reticulation and a high crystalline structure (IC = 82.3%). This sustainable and simple methodology presents a low-cost and efficient approach allowing the modulation of the surface properties of BC.
      原文链接:
    • NSTL
    • Elsevier

    Identification of favorable haplotypes/alleles and candidate genes for three plant architecture-related traits via a restricted two-stage multilocus genome-wide association study in upland cotton

    Wang C.Xie X.Yang D.Su J....
    12页
    查看更多>>摘要：? 2021 Elsevier B.V.Plant architecture-related traits are extremely crucial for cotton plants and are closely linked to their ability to adapt to reasonably close planting and to their suitability for mechanized harvesting. However, the genetic basis for cotton plant architecture-related traits remains poorly understood. To identify favorable haplotypes/alleles (FHs/FAs) and candidate genes for target traits, we performed a restricted two-stage multilocus genome-wide association study (RTM-GWAS) using a natural population consisting of 315 upland cotton accessions and 9244 single-nucleotide polymorphism linkage disequilibrium blocks (SNPLDBs), which were explored and established using the specific-locus amplified fragment sequencing (SLAF-seq) technique. Totals of 68, 34 and 55 significant SNPLDB loci associated with the plant height (PH), fruit branch angle (FBA) and fruit branch length (FBL), respectively, were identified via the RTM-GWAS method. We then estimated the allele effects of the significant loci associated with the target traits and further generated SNPLDB allele matrices to provide a brief inheritance constitution of the population. Among the significant SNPLDB associations, five involved in four major loci (LDB_1_21982826, LDB_9_51578589_51578819, LDB_16_37952328 and LDB_19_52309050_52309284) for the PH or FBL were concurrently detected in a multiple-environment combined model and in at least one single-environment model. A comparison of FHs/FAs frequencies for the four major SNPLDB loci revealed that the frequencies of FHs or FAs that were hidden in short and compact cotton accessions were much higher than those in tall and noncompact cotton accessions. We also observed that cotton accessions from the northern super-early-maturation region (NSER) had relatively short and compact plants compared with those from the Yangtze River region (YZRR) and northwestern inland region (NIR), and the high FA frequencies of the four major SNPLDB loci were consistent with the relatively short and compact plant architecture in five different geographic regions. In the neighboring genome fragments of the four major SNPLDB loci, ultimately, the five candidate genes that were highly expressed during the early stages of the growth and were responsible for cotton PH and FBL through the auxin signaling pathway were explored by RNA-seq data and bioinformatics analysis. This study identified FHs/FAs and candidate genes for PH and FBL, which could provide key insights into the genetic basis for the plant architecture of upland cotton and could promote the breeding of new cotton varieties with ideal plant architecture suitable for mechanized harvesting.
      原文链接:
    • NSTL
    • Elsevier

    Improvement of salt tolerance in Stevia rebaudiana by co-application of endophytic fungi and exogenous spermidine

    Bahari Saravi H.Gholami A.Baradaran Firouzabadi M.Asghari H....
    12页
    查看更多>>摘要：? 2021 Elsevier B.V.Stevia (Stevia rebaudiana Bertoni) is an important and salt-sensitive medicinal plant used as a natural sweetener in food products. In this work, we used a pot experiment to investigate the role of microbial inoculation and exogenous polyamine spermidine (Spd) in conferring salt stress tolerance in stevia by analyzing plant growth, chlorophyll (Chl) content, Chl a fluorescence, antioxidant defense system and steviol glycosides. The inoculated plants with Piriformospora indica (Pi), Tricuderma virens (Trich) or Pi+Trich were grown in optimum conditions. At the late of vegetative stage (BBCH growth stage 47), plants sprayed with different concentrations of Spd (0, 0.75, and 1.5 mM) and then were exposed to salinity stress by irrigation with saline treatments (mix of Caspian Sea and distilled water; 0, 6, and 12 dS m?1). The results revealed that salt stress increased hydrogen peroxide (H2O2) and lipid peroxidation (Malondialdehyde: MDA), and consequently plant growth characteristics and maximum photochemical quantum yield of PSII (Fv/Fm) as well as Chl content decreased, but antioxidant enzymes activity including superoxide (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) increased in the plants. Exposure to moderate salinity increased stevioside (Stv) and rebaudioside A (Reb A) content, while these compounds were decreased in the presence of severe salinity. The endophytic symbiosis and foliar spray of Spd (especially in Pi+Trich and Spd 0.75 mM) mitigated salt stress by significant increases in antioxidant enzymes activity and a decrease in H2O2 content and lipid peroxidation. Inoculation with Pi, Trich, and especially Pi+Trich increased the Chl content, Fv/Fm, shoot dry weight, and Stv and Reb A content at all salinity levels. Consequently, combined inoculation of Pi and Trich and foliar application of Spd can be a good approach to improve tolerance, growth and steviol glycosides production of stevia in salt stress conditions.
      原文链接:
    • NSTL
    • Elsevier

    Barrier packaging solutions from residual biomass: Synergetic properties of CNF and LCNF in films

    Trifol J.Moriana R.
    6页
    查看更多>>摘要：? 2022 The AuthorsIn this paper, for the first time, it is studied the synergetic properties of two different grades of nanocelluloses with different chemical compositions (cellulose nanofibrils-CNF with less than 1% of lignin and lignocellulose nanofibrils-LCNF with 16% of lignin). CNF and LCNF were mixed in different ratios to obtain bi-component films. Their performance in terms of transparency, bioactivity, thermo-mechanical and gas barrier properties was evaluated and compared with the performance of the neat CNF films. The presence of LCNF in the formulations conferred antioxidant and UV blocking properties to the films, as well as improved mechanical and barrier properties. Specifically, the incorporation of 25% LCNF to the CNF films increased the mechanical properties (94% increase in tensile stress and a 414% increase in strain at break) and decreased the water vapor transmission rate by 16% and the oxygen transmission rate by 53%. This performance improvement was attributed to the coexistence of nanocelluloses with different chemical composition and morphology. LCNF contributed to increment the interfacial adhesion between cellulose nanofibrils due to the presence of lignin and promote the creation of more tortuous paths for gas molecules. These synergetic properties shown by the CNF/LCNF bi-component films demonstrate high potential to be used as gas barrier packaging solutions.
      原文链接:
    • NSTL
    • Elsevier