首页期刊导航|Journal of Asia-Pacific Entomology
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Journal of Asia-Pacific Entomology
Elsevier
Journal of Asia-Pacific Entomology

Elsevier

1226-8615

Journal of Asia-Pacific Entomology/Journal Journal of Asia-Pacific EntomologySCI
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    Predation rates of mixed instar Odonata naiads feeding on Aedes aegypti and Armigeres moultoni (Diptera: Culicidae) larvae

    Weterings, RobbieUmponstira, ChaninBuckley, Hannah Linda
    8页
    查看更多>>摘要：In Thailand, several important diseases are transmitted by mosquitoes. Many vector control programs focus on the reduction of these medically important mosquitoes through the application of pesticides, bed-nets and the introduction of biological control agents. Odonates naiads are important, naturally occurring predators of vector mosquitoes. To estimate the predation rates of odonate species in Thailand, we conducted an experiment in which the predation rates were compared across a range of predator and prey densities. We used seven different predator species from different instars that represented the composition of naiads in our study area. Body sizes ranged between 2.6 mm and 15.9 mm. Two different prey species were used, larvae of the mosquito Armigeres moultoni Edwards, 1914 and Aedes aegypti L 1762. Predation rates showed a positive non-linear relationship with prey densities and a negative non-linear relationship with predator densities. The mean +/- SE predation rates per predator were 6.2 +/- 0.8 individuals per 24 h for dragonfly naiads and 5.1 +/- 0.7 for damselfly naiads. Predation rates were very low compared to previously recorded rates. However, unlike previous research, we did not focus on single species in a late stage of developmentbut on multiple species in all stages that resembled the natural odonata community composition. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Optimization of detection of black queen cell virus from Bombus terrestris via real-time PCR

    Choi, Na RaeJung, ChuleuiLee, Dae-Weon
    4页
    查看更多>>摘要：The bumblebee, Bombus terrestris, plays an important role as one of alternative pollinators since the outbreak of honeybee colony collapse disorder. Recently, pathogens and parasites such as viruses, bacteria and mites affecting the life span and fecundity of their host have been discovered in B. terrestris. In this study, in order to detect viral infection in B. terrestris, we collected B. terrestris adults and isolated total RNA for diagnostic PCR. The PCR primers specific for pathogenic viruses were newly designed and applied to gene amplification for cloning and detection. Capsid protein gene of black queen cell virus (BQCV) among examined viral genes was only successfully amplified from collected bumblebee adults and sequenced. To optimize the detection of capsid protein gene of BQCV, 4 regions in the capsid protein gene were selected and further analyzed in quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis revealed that capsid protein gene was directly detected with below 200 ng total RNA. This result suggests that an optimized detection via qRT-PCR can be applied for the rapid and sensitive diagnosis of BQCV infection in the field population as well as risk assessment of B. terrestris. (C) 2014 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    AvCystatin, a novel cysteine protease inhibitor from spider (Araneus ventricosus) venom

    Wan, HuKang, TinghaoKim, Bo YeonLee, Kwang Sik...
    6页
    查看更多>>摘要：Cystatins are involved in various physiological and cellular processes, including immune responses, protein homeostasis, signaling pathways, and apoptosis. Thus far, no Cystatins have been identified from spider venom. In this study, we report the cloning and characterization of a spider venom-derived Cystatin from Araneus ventricosus (AvCystatin). The AvCystatin gene contains an open reading frame of 405 bp encoding a predicted protein of 134-amino acids with a 16-amino acid signal peptide. Sequence alignment and structural modeling indicated that AvCystatin is closely related to family 2 Cystatins. Endogenous AvCystatin was present as an 18-kDa peptide in spider venom. Recombinant AvCystatin, expressed in baculovirus-infected insect cells, showed inhibitory activity against papain (K-i 86.73 nM), but not trypsin and chymotrypsin, defining a role for AvCystatin as a spider venom-derived cysteine protease inhibitor. Furthermore, recombinant AvCystatin exhibited stability against high temperatures and pH extremes, but had no effects on the growth of the entomopathogenic fungi Beauveria bassiana. These data demonstrate that AvCystatin is a novel member of the family 2 Cystatins and provide new insight for the future investigations of spider venom-derived Cystatins. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Functional characterization of sugar receptors in the western honeybee, Apis mellifera

    Jung, Je WonPark, Kun WoongAhn, Young-JoonKwon, Hyung Wook...
    8页
    查看更多>>摘要：The sense of taste is responsible for the choice of profitable food sources and nest mate recognition in honeybees. Taste detection for food sources occurs within cuticular hairs located on the antennae, on the mouthparts, and on the tarsi of the forelegs. The gustatory sensilla, which are composed of cuticular hair, respond to sugars, salts, and amino acids. In the honey bee, although sugar detection is a crucial factor in determining the acceptability of nectar and pollen for collection, little is known about the molecular and neural correlates underlying sugar perception. Here we identified the function of sugar receptors in Apis mellifera that is most closely related with sugar receptors in Drosophila melanogaster such as DmGr64a-f and DmGr5a. We determined that gustatory receptor 1 of A. mellifera (AmGr1) responded to sucrose, glucose, trehalose, and maltose in a dose-dependent manner, and that the expression patterns of AmGr1 and AmGr2 are distinct from those in Drosophila. AmGr1 alone showed full functionality, but it showed different sensitivity from a heterodimer of AmGr1 and AmGr2. That is, co-expression of AmGr1 and AmGr2 demonstrated higher sensitivity to glucose and lower sensitivity to sucrose, trehalose, and maltose compared with AmGr1 expression alone. AmGr1 and AmGr2 were co-localized or not in the antennal neurons, and especially AmGr1 was highly expressed at the distal segment of the antennae. Our study suggests that sugar receptors of the honey bee function as heterodimers (or monomer or mono-dimers), indicating that AmGr2 is required for providing honeybees with variability of sugar perceptions. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Calreticulin in Cotesia plutellae suppresses immune response of. Plutella xylostella (L.)

    Cha, Wook HyunKim, YonggyunLee, Dae-Weon
    5页
    查看更多>>摘要：An endoparasitoid wasp, Cotesia plutellae, parasitizes young larvae of the diamondback moth, Plutella xylostella, which is a major pest in cruciferous crops. Successful parasitization requires both maternal and embryonic factors of C. plutellae, such as polydnavirus, ovarian proteins, teratocytes and venom proteins. In this study, we identified calreticulin (Cp-CRT) gene from transcriptome data of the venom gland in C plutellae which encodes 403 amino acids harboring several structural motifs such as CRT family motif I and II, repetitive sequence (DP(X)(3)KPEDW), and endoplasmic reticulum-recognizing domain (-HDEL). Phylogenetic analysis showed that the Cp-CRT gene formed a unique cluster with other hymenopteran CRT genes. To examine the physiological function of Cp-CRT, recombinant Cp-CRT, fused with 6X-His at N-terminus, was expressed in Escherichia coli. Recombinant Cp-CRT was successfully expressed and suppressed significant nodule formation when co-injected with E. coli as immune response inducer. These results suggest that the Cp-CRT contributes to suppression of cellular immune response in the host. (C) 2014 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Effects of habitat specialization on population genetic structure of black fly Simulium weji Takaoka (Diptera: Simuliidae)

    Pramual, PairotPangjanda, Sirinapa
    5页
    查看更多>>摘要：Ecologically specialized species often show disjunctive distribution because suitable habitats have patchy occurrence. Consequently, a high level of genetic differentiation is expected because gene flow is limited. In this study, we examined the population genetic structure of the habitat-specialized black fly Simulium weji using mitochondrial cytochrome c oxidase subunit II sequences. A high level of genetic structure was observed with all F-ST values showing highly significant differences. The median joining haplotype network revealed two genetically divergent lineages that associated with the different microhabitats. Therefore, our results indicate the important role of the ecological conditions in the habitats on black fly evolution. As the genetic differentiation has not been observed in previous cytogenetic studies, our results support an integrated approach as being necessary for black fly biodiversity assessments. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Cold hardiness characteristic of the overwintering pupae of fall webworm Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) in the northeast of China

    Xu, Xiao-RuiZhu, Ming-MingLi, Liang-LiangZhang, Guang-Cai...
    7页
    查看更多>>摘要：The fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae), is an invasive and important pest in China. Understanding the physiological-biochemical characteristics of the overwintering pupae is essential for its cold tolerance and potential distribution in the northeast of China. Results indicated that the supercooling point (SCP) of H. cunea overwintering pupae varied significantly among Jilin city (43 degrees N, 126 degrees E), Jilin Province, Shenyang (41.8 degrees N, 123.4 degrees E), Liaoning Province and Yingkou (40.39N, 122.13E), Liaoning Province. The average SCP in Jilin was the lowest (-23.49 +/- 0.46 degrees C) in those of three areas. The water content in overwintering pupae had a significant positive correlation with the SCP (R = 0.848,P = 0.03). Contents of alanine (ALA) (26.00%), lysine (LYS) (30.51%) and arginine (ARG) (22.88%) in the overwintering pupae's hemolymph were higher than others. During the overwintering, the fat content in pupae's bodies decreased gradually. Analysis of variance (ANOVA) indicated that the content of trehalose in the body of H. cunea was remarkably different (P = 0.007) month by month, with the maximum in March (2.88 mg.mL(-1)) and the minimum (0.40 mg.mL(-1)) in November. Pearson's correlation test indicated that SCP had a significant negative correlation with the SOD enzyme activity (R = 0.850; P = 0.008). Thermal hysteresis activity (THA) of hemolymph was 0.68 degrees C and 0.7-0.8 degrees C for H. cunea pupae and larva, respectively, using differential scanning calorimetry, which suggests that the H. cunea can improve their cold tolerance through producing antifreeze proteins. These results could help us to predict the distribution of the H. cunea in the northeast of China. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Termiticidal activity of Acorus calamus Linn. rhizomes and its main constituents against Coptotermes curvignathus Holmgren

    Adfa, MorinaLivandri, FioMeita, Neva PutriManaf, Syalfinaf...
    4页
    查看更多>>摘要：The termiticiclal activity of n-hexane and methanol fractions of Acorus calamus Linn rhizomes against Coptotermes curvignathus Holmgren was investigated. Both fractions have demonstrated strong termiticidal activity. The activity of n-hexane fraction was stronger than that of the methanol fraction. Based on GC-MS data,beta-asarone was found to be a major constituent of n-hexane fraction (70.1%) and isolation of beta-asarone was carried out on column chromatography to afford as a yellow oil in 59.1% yield. Furthermore, the antitermite properties of beta-asarone and alpha-asarone of the isomer were investigated. Our data suggested that the two asarone isomers have insect control potential. These findings lead to the discovery of more environmentally friendly termiticides against C. curvignathus. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Flower and leaf damage of grapevines caused by the grape rust mite, Calepitrimerus vitis (Nalepa)

    Lee, Sun KyungIm, Jae SeongJung, Jong KookKim, Dong-Hwan...
    4页
    查看更多>>摘要：The symptoms of damage on flower buds and leaves of grapevines caused by the grape rust mite, Calepitrimerus vitis (Nalepa) were examined in this study. The damaged flower buds displayed retarded growth and became dried up and some of the damaged flower clusters were curved. Not only symptom of damaged leaves such as malformed and bronzed leaves, but also retarded shoot growth was observed. Flower clusters (each flower cluster consisted of one main cluster and one side cluster) and leaves were collected to determine the relationship between degree of damage symptom's and density of the grape rust mite, which was measured by a modified washing and sieving method. The highest mean density (+/- SE) of C vitis was found to be 672.6 +/- 112.9 per flower cluster with the mid-level damage, while the lowest mean density was found to be 327.6 +/- 55.5 per flower cluster with the high level damage. This was probably due to significant dispersal of grape rust mites from the highly damaged flower buds. Significantly higher numbers of C vitis were found on the distorted leaves than on healthy ones. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology

    Life table parameters of Plodia interpunctella (Lepidoptera: Pyralidae) on four commercial pistachio cultivars

    Razazzian, SaraHassani, Mohammad RezaImani, SohrabShojai, Mahmoud...
    5页
    查看更多>>摘要：Life table parameters of Indianmeal moth, Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae), on four pistachio cultivars (Akbari, Ahmad-Aghaii, Fandoghi and Kalehghouchi) were studied in laboratory at 28 +/- 0.5 degrees C, 50 +/- 5% RH and a photoperiod of 16:8 h (L:D). The larvae successfully developed on all cultivars. The shortest and longest larval periods were recorded on Akbari and Kalehghouchi, respectively. The fecundity of females varied from 109.19 to 157.81 eggs on Kalehghouchi and Akbari, respectively. The adult longevity varied from 6.19 to 6.75 days on Fandoghi and Akbari, respectively. The intrinsic rate of increase (r(m)) ranged from 0.0961 to 0.1382 (female/female/day) on Kalehghouchi and Akbari, respectively. The mean generation time (T) was found 35.67 to 31.83 days on Kalehghouchi and Akbari, respectively. The Kalehghouchi cultivar was less suitable, and the Akbari cultivar was the most suitable host for P. interpunctella in comparison with other cultivars. These results provide important information on population growth potential of P. interpunctella on four commercial pistachio cultivars. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Korean Soc Applied Entomology