查看更多>>摘要：Nodulation and symbiotic nitrogen fixation are important factors that determine legume growth. A pot experiment was carried out to determine the effects of Zn-Pb contamination on nodule apoplast (cell walls and intercellular spaces) of bird's foot trefoil (Lotus corniculatus L.) that spontaneously colonized old calamine wastes. The plants were grown in pots filled with sterile calamine substrate (M, metal treated) or expanded clay (NM, untreated) and inoculated with calamine-derived Lotus-nodulating Bradyrhizobium liaoningense. Apoplast reorganization in the nodules was examined using specific dyes for cellulose, pectin and lignin detection, and immuno-histochemical techniques based on monoclonal antibodies against xyloglucan (Lm25), pectins (Jim5 and Jim7), and structural proteins (arabinogalactan protein -Lm14 and extensin -Jim12). Microscopic analysis of metal-treated nodules revealed changes in the apoplast structure and composition of nodule cortex tissues and infected cells. Wall thickening was accompanied by intensified deposition of cellulose, xyloglucan, esterified pectin, arabinogalactan protein and extensin. The metal presence redirected also lignin and suberin deposition in the walls of the nodule cortex tissues. Our results showed reorganization of the apoplast of cortex tissues and infected cells of Lotus nodules under Zn-Pb presence. These changes in the apoplast structure and composition may have created actual barriers for the toxic ions. For this reason, they can be regarded as an element of legume defense strategy against metal stress that enables effective functioning of L. corniculatus-rhizobia symbiosis on Zn-Pb polluted calamine tailings.
Xavier, Lucas R.Almeida, Felipe A.Pinto, Vitor B.Passamani, Lucas Z....
12页
查看更多>>摘要：Plant embryogenic cell culture allows mass propagation and genetic manipulation, but the mechanisms that determine the fate of these totipotent cells in somatic embryos have not yet been elucidated. Here, we performed label-free quantitative proteomics and phosphoproteomics analyses to determine signaling events related to sugarcane somatic embryo differentiation, especially those related to protein phosphorylation. Embryogenic calli were compared at multiplication (EC0, dedifferentiated cells) and after 14 days of maturation (EC14, onset of embryo differentiation). Metabolic pathway analysis showed enriched lysine degradation and starch/sucrose metabolism proteins during multiplication, whereas the differentiation of somatic embryos was found to involve the enrichment of energy metabolism, including the TCA cycle and oxidative phosphorylation. Multiplication related phosphoproteins were associated with transcriptional regulation, including SNF1 kinase homolog 10 (KIN10), SEUSS (SEU), and LEUNIG_HOMOLOG (LUH). The regulation of multiple light harvesting complex photosystem II proteins and phytochrome interacting factor 3-LIKE 5 were predicted to promote bioenergetic metabolism and carbon fixation during the maturation stage. A motif analysis revealed 15 phosphorylation motifs. The [D-pS/T-x-D] motif was overrepresented during somatic embryo differentiation. A protein-protein network analysis predicted interactions among SNF1-related protein kinase 2 (SnRK2), abscisic acid responsive element-binding factor 2 (ABF2), and KIN10, which indicated the role of these proteins in embryo genic competence. The predicted interactions between TOPLESS (TPL) and histone deacetylase 19 (HD19) may be involved in posttranslational protein regulation during somatic embryo differentiation. These results reveal the protein regulation dynamics of somatic embryogenesis and new players in somatic embryo differentiation, including their predicted phosphorylation motifs and phosphosites.
查看更多>>摘要：Canavalia rosea (Sw.) DC is a perennial twining herb distributed in the semi-arid and saline-alkali areas of coastal regions and has evolved halotolerance. In this study, we present the first comprehensive survey of the metal-lothionein (MT) gene family in C. rosea. MT proteins belong to a family of low-molecular-weight polypeptides with a high content of cysteine residues, which have an affinity to bind with heavy metal ions. MTs also play important roles in stress responses as reactive oxygen species (ROS) scavengers. A total of six CrMTs were identified in the C. rosea genome and classified into four subgroups by phylogenetic analysis. An analysis of the cis-acting elements revealed that a series of hormone-, stress-, and development-related cis-acting elements were present in the promoter regions of CrMTs. The expression of CrMTs also showed habitat-and environmental stress-regulated patterns in C. rosea. CrMT overexpression in yeast enhanced tolerance to heavy metals and ROS, as well as high osmotic and alkalinity stress, which is consistent with their predicted roles as metal-chelating proteins and ROS scavengers. Our results indicate that the CrMT genes might contribute to the detoxification of plants to metals and provide marked tolerance against abiotic stress. The expression patterns of CrMTs in C. rosea also indicate that CrMTs play important roles in this species' response to extreme environments on tropical islands and reefs, probably by improving the thermotolerance of C. rosea plants.
查看更多>>摘要：Reproductive development is critical for completion of plant life cycle and realization of crop yield potential. Reproductive organs comprise multiple distinctive or even transgenerational tissues, which are symplasmically disconnected from each other for protection and better control of nutrition and development. Cell wall invertases (CWINs) and sugar transporters are often specifically or abundantly expressed in these apoplasmic interfaces to provide carbon nutrients and sugar signals to developing pollens, endosperm and embryo. Emerging evidence shows that some of those genes were indeed targeted for selection during crop domestication. In this Opinion paper, I discuss the functional significance of the localized expression of CWINs and sugar transporters in reproductive organs followed by an analysis on how their spatial patterning may be regulated at the molecular levels and how the localized CWIN activity may be exploited for improvement of reproductive output.
查看更多>>摘要：Rice is generally sensitive to chilling stress, which seriously affects growth and yield. Since early in the last century, considerable efforts have been made to understand the physiological and molecular mechanisms underlying the response to chilling stress and improve rice chilling tolerance. Here, we review the research trends and advances in this field. The phenotypic and biochemical changes caused by cold stress and the physiological explanations are briefly summarized. Using published data from the past 20 years, we reviewed the past progress and important techniques in the identification of quantitative trait loci (QTL), novel genes, and cellular pathways involved in rice chilling tolerance. The advent of novel technologies has significantly advanced studies of cold tolerance, and the characterization of QTLs, key genes, and molecular modules have sped up molecular design breeding for cold tolerance in rice varieties. In addition to gene function studies based on overexpression or artificially generated mutants, elucidating natural allelic variation in specific backgrounds is emerging as a novel approach for the study of cold tolerance in rice, and the superior alleles identified using this approach can directly facilitate breeding.
查看更多>>摘要：The upcoming climate change presents a great challenge for plant growth and development being extremes temperatures among the major environmental limitations to crop productivity. Understanding the repercussions of these extreme temperatures is of high importance to elaborate future strategies to confront crop damages. Tomato plants (Solanum lycopersicum L.) are one of the most cultivated crops and their fruits are consumed worldwide standing out for their organoleptic characteristics and nutritional value. Tomato plants are sensitive to temperatures below 12 degrees C and above 32 degrees C. In this study, Micro-Tom cultivar was used to evaluate the effects of extreme temperatures on the plant of tomato and the fruit productivity and quality from the stressed plants, either exposed to cold (4 degrees C for three nights per week) or heat (32 degrees C during the day, seven days per week) treatments. Total productivity and the percentage of ripe fruits per plant were evaluated together with foliar stress markers and the contents of photosynthetic pigments and tocochromanols. Fruit quality was also assessed determining lycopene contents, total soluble solids, total acidity and ascorbate contents. High temperatures altered multiple physiological parameters indicating a moderate stress, particularly decreasing fruit yield. As a response to this stress, plants enhanced their antioxidant contents both at leaf and fruit level. Low temperatures did not negatively affect the physiology of plants with similar yields as compared to controls, suggesting chilling acclimation. Both high and low temperatures, but most particularly the former, increased total soluble solids contents indicating that temperature control may be used as a strategy to modulate fruit quality.
查看更多>>摘要：Elevated [CO2] can increase rice biomass and yield, but the degree of this increase varies substantially among cultivars. Little is known about the gene loci involved in the acclimation and adaptation to elevated [CO2] in rice. Here, we report on a T-DNA insertion mutant in japonica rice exhibiting a significantly enhanced response to elevated [CO2] compared with the wild type (WT). The root biomass response of the mutant was higher than that of the WT, and this manifested in the number of adventitious roots, the average diameter of roots, and total root length. Furthermore, coarse roots ( 0.6 mm) and thin lateral roots (<0.2 mm) were more responsive to elevated [CO2] in the mutant. When exposed to lower light intensity, however, the response of the mutant to elevated [CO2] was not superior to that of the WT, indicating that the high response of the mutant under elevated [CO2] was dependent on light intensity. The T-DNA insertion site was located in the promoter region of the OsGF14b gene, and insertion resulted in a significant decrease in OsGF14b expression. Our results indicate that knockout of OsGF14b may improve the response to elevated [CO2] in rice by enhancing carbon allocation to coarse roots and to fine lateral roots.
Fernandez, Javier A.Nippert, Jesse B.Prasad, P. V. VaraMessina, Carlos D....
11页
查看更多>>摘要：Nitrogen (N) metabolism is a major research target for increasing productivity in crop plants. In maize (Zea mays L.), yield gain over the last few decades has been associated with increased N absorption and utilization efficiency (i.e. grain biomass per unit of N absorbed). However, a dynamical framework is still needed to unravel the role of internal processes such as uptake, allocation, and translocation of N in these adaptations. This study aimed to 1) characterize how genetic enhancement in N efficiency conceals changes in allocation and trans location of N, and 2) quantify internal fluxes behind grain N sources in two historical genotypes under high and low N supply. The genotypes 3394 and P1197, landmark hybrids representing key eras of genetic improvement (1990s and 2010s), were grown under high and low N supply in a two-year field study. Using stable isotope N-15 labelling, post-silking nitrogen fluxes were modeled through Bayesian estimation by considering the external N (exogenous-N) and the pre-existing N (endogenous-N) supply across plant organs. Regardless of N availability, P1197 exhibited greater exogenous-N accumulated in leaves and cob-husks. This response was translated to a larger amount of N mobilized to grains (as endogenous-N) during grain-filling in this genotype. Furthermore, the enhanced N supply to leaves in P1197 was associated with increased post-silking carbon accumulation. The overall findings suggest that increased N utilization efficiency over time in maize genotypes was associated with an increased allocation of N to leaves and subsequent translocation to the grains.
查看更多>>摘要：In the early growth stage, nutrient uptake by rice roots is weak. However, rice tillering at this stage would require high N input. Thus, it is vital to clarify the mechanism involved in tillering capacity with low N inputs. In this report, two widely-planted japonica cultivars (cvs Yangyujing 2 and Nanjing 45) were selected mainly because, unlike cv. Nanjing 45, cv. Yangyujing 2 shows low-N-induced tiller outgrowth. Responses of tillers in two rice cultivars to mixture of N forms versus sole NH4+ supply were similar, suggesting that NH4+ plays a pivotal role in N-modulated rice tillering. Under low NH4+ supply, higher expression of OsAMT1.2, OsAMT1.3, OsGS1;2, and OsGS2 was recorded in the roots of cv. Yangyujing 2 in comparison with cv. Nanjing 45, ultimately resulting in higher N content and dry weight in cv. Yangyujing 2. Stronger H-3-IAA export from tiller stems was observed in cv. Yangyujing 2, mainly due to higher expression level of auxin efflux transporters. Moreover, tillers in auxin efflux transporter mutant ospin9 did not respond to NH4+ supply relative to wild-type plants. These findings can be used in the molecular breeding of rice varieties to simultaneously improve rice population productivity and reduce N fertilizer input.
Moreno-Garcia, BeatrizLopez-Calcagno, Patricia E.Raines, Christine A.Sweetlove, Lee J....
13页
查看更多>>摘要：The communication between chloroplasts and mitochondria, which depends on the inter-organellar exchange of carbon skeletons, energy, and reducing equivalents, is essential for maintaining efficient respiratory metabolism and photosynthesis. We devised a multi-transgene approach to manipulate the leaf energy and redox balance in tobacco (Nicotiana tabacum) while monitoring the in vivo cytosolic redox status of NAD(H) using the biosensor cPeredox-mCherry. Our strategy involved altering the shuttling capacity of the chloroplast by (1) increasing the chloroplast malate valve capacity by overexpression of the chloroplast malate valve transporter pOMT from Arabidopsis (AtpOMT1) while (2) reducing the activity of the chloroplast triose-phosphate/3-phosphoglycerate shuttle by knocking down the cytosolic NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (NtGAPC). This was accompanied by (3) alterations to the export of reducing equivalents in the mitochondria by knocking down the mitochondrial malate dehydrogenase (NtmMDH) and (4) an increased expression of the mitochondrial fission regulator FIS1A from Arabidopsis (AtFIS1A). The multi-transgene tobacco plants were analysed in glasshouse conditions and showed significant increases in the cytosolic NADH:NAD+ in the dark when transcript levels for NtGAPC or NtmMDH were knocked down. In addition, principal component analysis and Spearman correlation analyses showed negative correlations between average transcript levels for the gene targets and parameters related to chlorophyll fluorescence and plant growth. Our results highlight the importance of the shuttling of energy and reducing equivalents from chloroplasts and mitochondria to support photosynthesis and growth and suggest an important role for the dual 2-oxoglutarate/malate and oxaloacetate/ malate transporter (pOMT).
NSTL
Elsevier