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Food Control
Elsevier Science Ltd.
Food Control

Elsevier Science Ltd.

0956-7135

Food Control/Journal Food ControlSCIISTP
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    Desorption of nutrients and flavor compounds formation during the cooking of bone soup

    Meng, QianZhou, JianweiGao, DeXu, Enbo...
    11页
    查看更多>>摘要:In order to identify the effects of varying cooking time on the quality of bone soup, the diversification of nutrient profiles and flavor compounds in bone soup prepared within 10 h' cooking was studied. The Kjeldahl method, inductively coupled plasma mass spectrometry (ICP-MS), automatic amino acid analyzer (AAA analyzer), high performance liquid chromatography (HPLC), solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) were applied to determine the changes of crude protein, minerals, free amino acids (FAAs), 5 '-nucleotides, and volatile compounds in the soup, respectively. During the 10 h cooking, the concentration of nutrients and flavor compounds reached their maximum and then decreased, except for minerals, which continued to increase in concentration. 18 FAAs were identified in all samples, which were related with Icollagen hydrolysis. Taste FAAs contributed little to the flavor of soup. Bitter amino acids increased significantly (P < 0.05) and IMP + GMP reached maximum concentration at the seventh hour of cooking. The main flavor compounds of bone soup were aldehydes, which increased significantly (P < 0.05) during boiling. Samples taken at the fourth and fifth hours contained the richest profile of volatile flavor compounds. In general, for highquality bone soup, cooking time should be limited to achieve an appropriate balance between nutrient dissolution and flavor formation.

    Situating curriculum in context: Using Glatthorn's Standards-Based Curriculum Development Model to contextualize food safety learning competencies

    Limon, Mark R.Vallente, John Paul C.Chua, Consuelo T.Rustia, Abigail S....
    11页
    查看更多>>摘要:Despite the increasing cases of foodborne disease outbreaks involving high school students in the Philippines, the country's basic education lacks a dedicated space for food safety education (FSED). This study problematizes such an issue where education and health intersect. Stemming from benchmarking efforts conducted in the United States of America (USA), FSED learning competencies, specifically intended for Filipino junior high school (JHS) students, were formulated using Glatthorn's Standards-Based Curriculum Development Model. A researcher-developed instrument with Cronbach Alpha of .96 and an interview protocol were employed to collect data from 40 foreign and 100 local participants. These data were analyzed using content analysis, descriptive statistics, and Fleiss' kappa statistics. It was found that USA middle and high schools demonstrated outstanding curricular and pedagogical practices in FSED concerning learning objectives, number of teaching hours, content description and focus, instructional activities, assessment methods, educational resources, and required certifications and trainings. These variables were considered in formulating contextualized learning competencies in food safety for JHS students. Results of the statistical analysis yielded weighted mean, standard deviation, and Fleiss' kappa of 1.00 +/- 1.19 (Kappa = 0.97), 1.00 +/- 1.23 (Kappa = 0.96), 1.00 +/- 1.62 (Kappa = 0.94), and 1.33 +/- 1.38 (Kappa = 0.96) in personal health and hygiene, hazards in food, cleaning and sanitation, and good manufacturing practices, respectively. With overall rating of 1.08 +/- 1.04 (Kappa = 0.96 at p < .05), topics embedded in the learning competencies are considered to be highly beneficial. It is recommended that these contextualized and localized learning competencies are incorporated into the country's present K to 12 Curriculum to educate the Filipino youth about food safety.

    Isolation, purification, identification, and discovery of the antibacterial mechanism of LD-phenyllactic acid produced by Lactiplantibacillus plantarum CXG9 isolated from a traditional Chinese fermented vegetable

    Zhang, ChengchengLei, PengXin, XiaotingLiu, Daqun...
    9页
    查看更多>>摘要:Phenyllactic acid (PLA) is an antimicrobial compound and has been rarely reported to naturally occur in food sources with the exception of honey, sourdough, and some pickles. In this study, a traditional Chinese fermented vegetable, Stinky xiancaigeng, was found to contain high concentrations of PLA. A high PLA level-producing Lactiplantibacillus plantarum (L. plantarum) CXG9 was subsequently isolated and subjected to genomic analysis. Six L-and two D-lactate dehydrogenase genes were predicted, indicating that PLA produced by L. plantarum CXG9 contained L-and D-isomers. The ratio of L-and D-isomers was 11:1 by chiral high performance liquid chromatography analysis. LD-PLA from L. plantarum CXG9 cultures were then purified by a three-step procedure and identified by mass spectrometry. LD-PLA exhibited strong anti-Listeria monocytogenes activity, with a minimum inhibitory concentration of 2.4 mg/mL. The results of anti-Listeria monocytogenes mechanism demonstrated that LD-PLA damaged cell membrane integrity, increased membrane permeability, induced pore formation, and triggered intracellular material leakage by interacting with cell membrane proteins. These results presented here contribute to the use of PLA in further development and application as an antibacterial agent in the food industry.

    Critical comparison of direct analysis in real time orbitrap mass spectrometry (DART-Orbitrap MS) towards liquid chromatography mass spectrometry (LC-MS) for mycotoxin detection in cereal matrices

    Tsagkaris, A. S.Hrbek, VDzuman, Z.Hajslova, J....
    9页
    查看更多>>摘要:Foodstuffs contaminated by mycotoxins have been reported around the globe resulting in significant financial losses and health-threating cases due to dietary exposure. Commonly, two analytical approaches are used in mycotoxin analysis, namely screening bioanalytical methods such as immunoassays and confirmatory chro-matographic analysis which are related to certain merits and challenges. In this study, to provide an alternative combining rapidness and unequivocal analyte identification, a direct analysis in real time ion source coupled to orbitrap mass spectrometry (DART-Orbitrap MS) method was developed and validated for the detection of 19 mycotoxins in cereal flour matrices. Excellent detectability expressed as the lowest calibration level (LCL) ranging between 25 mu g kg-1 and 250 mu g kg-1 depending on the analyte was achieved as well as satisfactory precision and trueness. To identify advantages and drawbacks of the in-house DART-Orbitrap MS method against the golden standard methods, a critical comparison towards liquid chromatography (LC) coupled to high and low resolution MS was performed. DART-Orbitrap MS high throughput and rapidity was revealed while LC-MS proved its superior detectability, which is of indispensable importance for mycotoxin regulatory control. In conclusion, the developed DART-Orbitrap MS method can be applied to intensify and accelerate the monitoring of specific mycotoxins in cereal matrices.

    Differences in code terminology and frequency of findings in meat inspection of finishing pigs in seven European countries

    Vieira-Pinto, MadalenaMeemken, DianaMaurer, PatricGhidini, Sergio...
    13页
    查看更多>>摘要:The overall objectives of meat inspection are to contribute to food safety, animal welfare, and animal health. In the European Union (EU), there is a request for a modernised meat inspection system that addresses these objectives in a more valid, feasible and cost-effective way than does the traditional system. One part of the modernisation deals with the coding system to register meat inspection findings. Although unified standards are set at the EU level for judgement criteria regarding fitness of meat for consumption, different national systems are in force. The question is the extent of the differences and whether there is a basis for harmonisation. To investigate this, information was gathered about the code systems in Denmark, Finland, Germany, Italy, Norway, Portugal and Spain. Moreover, meat inspection data covering pigs slaughtered in 2019 were collected. A comparison of the number of codes available, the terminology and the frequencies of the findings registered was undertaken. Codes with a similar meaning were grouped. Hereby, two lists were compiled showing the most common codes leading to total and to partial condemnation. Substantial variations in the percentage of condemned pigs and in the terms used were identified, and possible reasons behind this are discussed. Moreover, a strengths-weaknesses-opportunities-threats (SWOT)-like analysis was applied to the coding systems. Finally, the reasons for unfitness of meat given in the EU Food Inspection Regulation 2019/627 were compared to the national code lists. The results show the systems in force varied substantially, and each system had its advantages and disadvantages. The diverse terminology observed made it a challenge to compare data between countries. Development of harmonised terminology for meat inspection findings is suggested, enabling comparison of data between abattoirs, regions, and countries, while respecting the national epidemiological situation, the local food safety culture, and the trade agreements in force.

    Determination of sulfur dioxide in food by liquid chromatography with pre-column derivatization

    Mu, LiLi, GangKang, QinXu, Ying...
    10页
    查看更多>>摘要:A derivatization method to determine the sulfur dioxide content in food was established. Under weakly acidic conditions, fluorescent 2H-isoindole-1-sulfonate was synthesized by a derivatization reaction using an ammonium salt and o-phthalaldehyde as the derivative reagent. High-performance liquid chromatography and a highsensitivity fluorescence detector were used for detection, in which a fluorescence spectrophotometer was used to determine the maximum excitation wavelength and emission wavelength of2H-isoindole-1-sulfonate. When the excitation wavelength was 323 nm and the emission wavelength was 386 nm, the linear range of fluorescence intensity of the sulfur dioxide standard was 0-10 mu g/mL, and the correlation coefficient was 0.996. The standard recoveries of six kinds of food were approximately 80-110%, the RSD was 0.2-2.89, the detection limit was 0.2 mg/kg, and the quantitative limit was 0.7 mg/kg. The results of this research indicate that this method is suitable for determining sulfur dioxide in food.

    Colorimetric immunoassay via smartphone based on Mn2+-Mediated aggregation of AuNPs for convenient detection of fumonisin B1

    Yu, YaoLi, YansongZhang, QiZha, Yonghong...
    8页
    查看更多>>摘要:Fumonisin B1 (FB1) is one of the most toxic and widely distributed subtypes of the 20 fumonisins, which has attracted much attention in food and feed safety. In this study, a convenient colorimetric immunoassay was proposed for the detection of FB1 based on Mn2+-mediated aggregation of gold nanoparticles (AuNPs). After hydrolyzing of ascorbic acid 2-phosphate (AAP) by alkaline phosphatase (ALP), the generated ascorbic acid (AA) decomposes brown MnO2 shell to Mn2+, which growth onto AuNPs and inducing the aggregation of AuNPs. The color change of the solution induced by aggregating of AuNPs can be read by smartphone to avoid subjective differences. The limit of detection (LOD) of this assay was 0.15 ng/mL and the limit of quantification (LOQ) was 0.50 ng/mL. The method has not cross-reaction with other mycotoxins. The average recoveries of FB1 in the maize flour samples ranged from 86.4 % to 110.9 %, which met the food and Drug Administration (FDA) restrictions on FBS in agricultural products. The colorimetric immunoassay can be potentially used for mycotoxin-related food safety monitoring.

    Identification of coriander oil adulteration using a portable NIR spectrometer

    Kaufmann, Karine CristineSampaio, Klicia AraujoGarcia-Martin, Juan F.Barbin, Douglas Fernandes...
    7页
    查看更多>>摘要:Coriander oil is a vegetable oil extracted from coriander seed that has about 70% of petroselinic acid, apart from anti-inflammatory and anti-aging properties, thus gaining the status of new food ingredient. Due to its properties and added value, it can become the target of adulteration as occurs with other edible vegetable oils of high market value. Therefore, the objective of this work was to identify the authenticity of coriander oil and adulteration with other commercial vegetable oils such as palm olein, canola oil and soybean oil. Principal component analysis (PCA) differentiated the matrices of pure oils using 3 principal components, which explained 87% of the variance. Linear discriminant analysis (LDA) and k-nearest neighbors algorithm (k-NN) were used to classify pure oil samples and adulterated coriander oils. Partial Least Squares (PLS) regression models presented coefficient of determination (R2) of 0.98, 0.99 and 0.99, for coriander oil adulterated with palm olein soybean oil and canola oil, respectively. RPD was between 7.1 and 10, which indicates robust models that can be used for quality control during the processing of coriander oil.

    Antibacterial mechanism of linalool against L. monocytogenes, a metabolomic study

    He, RongrongChen, WeijunChen, HaimingZhong, Qiuping...
    12页
    查看更多>>摘要:Listeria monocytogenes (L. monocytogenes) is a highly pathogenic microorganism that can cause food contamination and a series of foodborne diseases. Linalool is widely used in the food processing industry due to its excellent antibacterial properties. But its antimicrobial mechanism against L. monocytogenes has scarcely been reported based on metabolomics technology. In this study, the activity of linalool against L. monocytogenes and its effect on cell membrane damage were investigated. Meanwhile, the activity of key enzymes in respiratory metabolism was determined. Moreover, untargeted metabolomics was applied to evaluate the inhibiting mechanism on L. monocytogenes. Our results indicated that linalool showed a strong inhibitory activity against L. monocytogenes by cell damage with a minimum inhibitory concentration (MIC) of 1.5 mL/L. In addition, the enzymes activity including pyruvate kinase (PK), succinic acid dehydrogenase (SDH), malic acid dehydrogenase (MDH) and respiratory chain dehydrogenase were significantly reduced, revealing that linalool can inhibit respiratory metabolism of L. monocytogenes. After treated with linalool, a significant change in metabolites was found based on multivariate statistical analysis, which involved in amino acid metabolism, central carbon metabolism, lipid metabolism, nucleic acid metabolism. Moreover, the disturbance of central carbon metabolism corresponded to the decrease in the activity of key enzymes in respiratory metabolism. These results provided a new insight into the mechanism of linalool inhibiting L. monocytogenes, and implied that linalool has the potential to prevent bacterial contamination by L. monocytogenes in the food industry.

    Synergistic effects of monocaprin and carvacrol against Escherichia coli O157:H7 and Salmonella Typhimurium in chicken meat preservation

    Ma, MaomaoZhao, JunxinYan, XianghuiZeng, Zheling...
    11页
    查看更多>>摘要:Escherichia coli O157:H7 and Salmonella Typhimurium are both foodborne pathogens commonly found in contaminated chicken meat. In this study, the combined effects of monocaprin and thymol, carvacrol, citral, and cinnamaldehyde against both pathogens were investigated to evaluate their effects in chicken meat preservation. Among the combinations, only the combination of monocaprin and carvacrol exhibited distinct synergistic effects on both pathogens in vitro. This combination showed a stronger synergistic biofilm inhibition and bactericidal effect than monocaprin or carvacrol alone. SEM and TEM microscopy showed that the combined treatment synergistically damaged the morphology of E. coli O157:H7 and S. Typhimurium. DAPI/SYTOX Green double-staining, outer and inner membrane permeability, membrane potential, and reactive oxygen species (ROS) generation experiments were performed to further assess the synergistic action. Results indicated that carvacrol rapidly increased the outer membrane permeability, allowing monocaprin to enter the cell of both pathogens, and damage the plasma membrane, and monocaprin increased the ROS accumulation effect of carvacrol, resulting in cell death. Monocaprin and carvacrol also showed synergistic effect on retarding the growth of E. coli O157:H7 and S. Typhimurium in chicken breast meat during storage at 4 degrees C. Besides, the results of sensory evaluation, including appearance, odor, firmness and overall acceptance, showed that the combined treatment reduced deterioration of the sensory properties of chicken breast meat. These findings suggest that this combination may be used as a novel approach to the preservation of chicken meat.