Sravya, V.Pavithra, V. R.Thangadurai, T. DanielNataraj, D....
9页
查看更多>>摘要：N-GQDs with an average size of ca. 20-30 nm are utilized for the picomolar detection of inhibitory neurotransmitters, glycine (Gly), in pH ca. 7.0. The crystalline nature, morphology, elemental composition, and chemical state of N-GQDs are investigated by XRD, FE-SEM, HR-TEM, XPS, and FT-IR techniques. The addition of Gly (100 x 10(-9) M; 0 -> 1.0 mL) steadily quenches the fluorescence intensity of N-GQD (1 x 10(-6) M) at 432 nm (lambda ex 333 nm) due to inner filter effect (IFE) through the formation of ground-state complex, N-GQD center dot Gly. The excitation-independent N-GQDs showed an outstanding selectivity and sensitivity towards Gly with binding constant (Ka = 8.97 x 10(-3) M-1) and LoD (21.04 pM; S/N = 3). Time-correlated single-photon counting experiment confirms the static quenching of N-GQD (8.77 -> 8.85 ns) in the presence of Gly. The interference of other amino acids on the strong binding of the N-GQD center dot Gly complex in H2O is examined. Combinatorial Ex-OR and NOT gate logic circuits that could be useful in neuromorphic computing are developed based on the reversible fluorescence intensity changes of N-GQD upon the addition of Gly (Phi(F) 0.54 -> 0.39). The real-time application of N-GQD was investigated using commercially available relevant milk samples. Remarkably, not less than 99% cytotoxic reactivity of N-GQDs is attained against HeLa cells.
查看更多>>摘要：Aflatoxin B-1 (AFB(1)) contamination is a severe threat to food safety and human health, and requires continuous monitoring. In this study, we developed a biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) by using biotinylated nanobody Nb26 and streptavidin-conjugated polymerized horseradish peroxide (SA-PolyHRP) for sensitive and rapid detection of AFB(1) in cereal. Under the optimal condition, the IC50 value of the BA-ELISA was improved to 0.21 ng mL(-1) for AFB(1), satisfying the requirement of detection limit in practical applications. The total assay time of our strategy is reduced to 50 min from 2 h in conventional competitive ELISA. Additionally, the BA-ELISA saves as much as 98% of the antibody in comparison to the previous classic ELISA. Our work also demonstrated an interesting phenomenon that the biotinylated Nb26 achieved better selectivity to AFB(1), which could possibly result from the steric hindrance that interferes reaction between the Nb26 and the AFB(1) analogs. Furthermore, the assay was used to detect AFB(1) in two cereal samples, and the results were in good agreement with that obtained by high performance liquid chromatography. The developed BA-ELISA can be used for routine screening analysis of AFB(1), and offers a promising strategy for measuring low concentrations of food contaminants.
查看更多>>摘要：The present scenario in the world largely demands affordable, fast, recyclable, and flexible electronic devices for bio sensing. Varieties of paper-based devices such as microfluidics paper electrodes, paper diodes, and paperbased transistors etc. have been developed and validated. Most of the fabrication techniques published so far have focused on economic, environment-friendly straightforward methods to develop paper-based field-effect transistors (PFET) sensors, additionally, explored their applications. The synthetic-free, mechanically flexible, biocompatible, and signal amplification capability render PFET based sensors for wearable device makers. Modified organic/inorganic PFETs identify target analytes based on the electrical signal and endow them as perfect transducers. In the field of PFET bio sensing technology, numerous challenges are needed to be discussed to proceed forward in biomedical and other analytical applications. Realizing biologically or chemically modified PFET having an exceptional signal to noise ratio, specificity, with rapid detection ability is challenging. This review recapitulates the fabrication techniques, performances of various PFET sensors, and summarizes the report by concluding remarks including the limitations of the existing PFET based sensors and the future holds in regards to the sustainable nature of PFET.
Andries, Jan P. M.Tinnevelt, Gerjen H.Vander Heyden, Yvan
8页
查看更多>>摘要：In this study, a new approach for PLS modelling for low-correlated multiple responses, called Common-Subset-ofIndependent-Variables Partial-Least-Squares, denoted as CSIV-PLS1, is proposed and evaluated. In CSIV-PLS1, for each response vector, individual PLS1 models with individual model complexities are developed, based on one common set of independent variables, obtained after variable selection by the Final Complexity Adapted Models method, using the absolute values of the PLS regression coefficients, denoted as FCAM-REG. CSIV-PLS1 combines a common variable set for all response vectors, which is a characteristic of PLS2, with the individual model complexity for each response, which is a characteristic of PLS1. These characteristics make CSIV-PLS1 more flexible than PLS2. The selective and predictive abilities of the proposed CSIV-PLS1 method are investigated using one simulated and four real data sets with low-correlated multiple responses from different sources. The simulated data set is used to test the general applicability of the CSIV-PLS1 method. The predictive abilities, measured by the RMSEP values, resulting from CSIV-PLS1 models, are statistically compared with those of the corresponding PLS1 and PLS2 models, using one-tailed paired t-tests. The selective ability of the CSIV-PLS1 method is good, because mostly variables with an informative meaning to the responses are selected. The RMSEP values resulting from the CSIV-PLS1 method are (i) significantly lower at the 95% confidence level than those of the corresponding PLS2 method, and (ii) borderline significantly lower at the 90-95% confidence level than those of the corresponding PLS1 methods. In case of low-correlated multiple responses, the predictive ability of the CSIV-PLS1 method is significantly better than that of the PLS2 method, and borderline significantly better than those of the corresponding PLS1 methods. Therefore, CSIV-PLS1 modelling may be an alternative for PLS1 or PLS2.
查看更多>>摘要：Chlortetracycline (CTC) is a widely used broad-spectrum antibiotic, its residue likely occurs in the environment and foods, bringing some negative effects to human health. Hence the detection and quantification of CTC in environmental and food samples is relevant. Herein, a novel electrochemical sensor based on surface molecularly imprinted polymer (SMIP) was constructed for the quantitative detection of CTC. The SMIP was synthesized by using ionic liquid (IL) functionalized MWCNT (MWCNT-IL) as supporter, 1-carboxymethyl-3-vinylimidazolium bromide (IL1) as functional monomer, CTC as template, ethylene glycol dimethyl acrylate as crosslinker, and azobisisobutyronitrile as initiator. The obtained composite IL1-SMIP exhibited high adsorption capacity for CTC and the imprinting factor was ca. 4.1. It was found that IL played an important role in improving the property of SMIP, which was also evaluated by DFT-based calculation. The resulting sensor IL1-SMIP/MWCNT-IL/GCE showed high selectivity, sensitivity and reproducibility. CTC could be quantified from 0.4 mu M to 55 mu M with a detection limit of 0.08 mu M (S/N = 3) under the optimized conditions. The practical applicability of the sensor was demonstrated successfully by determining CTC in real samples.
查看更多>>摘要：Changing a detection analyte into a colored material is a key challenge for visual discrimination of isomers. In this work, a novel fluorescent probe incorporating Cu-doped carbon dots (Cu-CDs), for the first time, was developed for naked-eye discrimination of phenylenediamine isomers and highly sensitive ratiometric fluorescence detection of H2O2. In this strategy, Cu-CDs were synthesized by a facile hydrothermal approach using citric acid, formamide, and CuCl2 as reactants. The prepared Cu-CDs exhibited outstanding peroxidase-like activity and stability. Consequently, a chemosensor platform based on Cu-CDs was constructed to enable naked-eye discrimination of phenylenediamine isomers through the H2O2-mediated oxidation reaction. Moreover, a Cu CDs-based ratiometric fluorescence sensor was proposed as a means to sensitively detect H2O2 with a detection limit of 5.0 nM. The sensor was further employed for monitoring H2O2 in human serum, indicating its potential applications in other biologically related study.
查看更多>>摘要：Through its Active Pharmaceutical Ingredient Working Group (API-WG) the General European Official Medicines Control Laboratory (OMCL) Network (GEON), co-ordinated by the European Directorate for the Quality of Medicines & HealthCare (EDQM), regularly organises market surveillance studies for specific APIs for conformity to their monograph in the European Pharmacopoeia. During the past years some studies were combined with a fingerprint study of the APIs. The idea is to obtain a fingerprint for each manufacturer of the API under investigation, allowing the OMCL network to identify future samples as well as to detect substandard and falsified APIs. This paper reports the results of the latest fingerprint study, organised on sildenafil citrate API samples. Seventy-nine samples from 14 different manufacturers were collected throughout the Network. Fingerprint data was collected through Mid-Infrared spectroscopy, Raman spectroscopy, liquid chromatography for related substances, gas chromatography for residual solvents, X-ray diffraction and Nuclear Magnetic Resonance (NMR) spectroscopy. Chemometrics applied to the collected data showed that all manufacturers could be discriminated based on the data of only three of these tests, i.e. gas chromatography for residual solvents, X-ray diffraction and proton NMR. Suspicious API samples for sildenafil citrate will therefore be analysed in the future with the selected techniques in order to link the sample to a manufacturer or demonstrate the absence of such link. If the sample cannot be attributed to one of the manufacturers, further analysis and research on provenance and identity will be required. Of course, if the suspected sample claims to originate from one of the manufacturers included in the study, analysis can be limited to the test distinguishing this manufacturer.
Safian, Muhammad Taqi-uddeenSekeri, Siti HajarYaqoob, Asim AliSerra, Albert...
19页
查看更多>>摘要：With each passing year, the agriculture and wood processing industries generate increasingly high tonnages of biomass waste, which instead of being burned or left to accumulate should be utilized more sustainably. In parallel, advances in green technology have encouraged large companies and nations to begin using eco-friendly materials, including eco-friendly emulsifiers, which are used in various industries and in bio-based materials. The emulsion-conducive properties of lignocellulosic materials such as cellulose, hemicellulose, and lignin, the building blocks of plant and wood structures, have demonstrated a particular ability to alter the landscape of emulsion technology. Beyond that, the further modification of their structure may improve emulsion stability, which often determines the performance of emulsions. Considering those trends, this review examines the performance of lignocellulosic materials after modification according to their stability, droplet size, and distribution by size, all of which suggest their outstanding potential as materials for emulsifying agents.
查看更多>>摘要：Ten-eleven translocation (TET) family proteins play key roles in multiple cellular processes by mediating the oxidation of 5-methylcytosine to directly participate in DNA demethylation, and often aberrantly expressed in various diseases. In this research, we develop a single-quantum-dot (QD)-mediated fluorescence resonance energy transfer (FRET) biosensor for amplification-free measurement of ten-eleven translocation 2 (TET2). When TET2 is present, it catalyzes the oxidation of 5-vinylcytosine in dsDNA to 5-formylmethylcytosine, and the subsequent labeling of dsDNA with Cy5 generates a biotinylated Cy5-dsDNA complex. Biotinylated Cy5-dsDNA complexes are conjugated to the streptavidin-coated 605QDs to obtain a Cy5-dsDNA-605QD nanostructures, inducing FRET from 605QD to Cy5. FRET signal can be simply measured by single-molecule counting. This biosensor enables homogeneous detection of TET2 with a limit of detection (LOD) of 0.042 ng/mu L, and it can accurately measure cellular TET2 down to 1 cell. Moreover, this biosensor can be used to screen TET2 inhibitors, offering a new platform for TET2-related medical research and clinical diagnostics.
Li, DanleiBatchelor-McAuley, ChristopherCompton, Richard G.
6页
查看更多>>摘要：An amperometric method was applied for the electroanalytical measurement of oxygen content in sheep's blood. This method was based on a bare platinum microdisc electrode coupled with the use of chronoamperometry. A linear relationship between the chronoamperometric current and the oxygen concentration was observed in both saline solution and sheep's blood. The developed method was able to measure the oxygen percentage with an error of ca. 1.3% in sheep's blood. In addition, this article presents the first study on direct voltammetry in sheep's blood and a dissociative CE process was proposed to explain the electrochemical behaviour of oxygen reduction in blood on a platinum electrode in which the 'free' oxygen was first dissociated from oxyhaemoglobin prior to electron transfer with the magnitude of the observed current controlled by the diffusion of oxyhaemoglobin to the electrode where for sufficiently large electrodes (greater than ca. 1 mu m in radius) the dissociation proceeds to completion on the voltammetric timescale allowing quantitative measurements.
NSTL
Elsevier