Ruiz-Medina, A.Jimenez-Lopez, J.Llorent-Martinez, E. J.
7页
查看更多>>摘要：We propose a modification of lanthanide-sensitized luminescence (LSL) to increase the selectivity and sensitivity of analytical methods based on this detection. LSL consists in the formation of complexes of lanthanide ions and organic compounds. Then, an intramolecular energy transfer occurs from the excited state of the ligand (organic analyte) to the emitting level of the lanthanide. The utilization of luminescent nanoparticles (carbon quantum dots, CQDs) in LSL systems can enhance their sensitivity and selectivity. CQDs can react with lanthanide ions through their carboxylic groups. These systems can thus be used as time-resolved luminescent probes. Propineb (PPN), a well-known dithiocarbamate fungicide, has been selected as the target analyte to show the advantages of using CQDs in LSL systems. The method proposed is based on the quenching produced by PPN in europiumCQDs luminescence, obtaining a detection limit of 0.03 mu g mL(-1) PPN and a method detection limit of 3 mg kg(-1) in capers (bud and fruit), fulfilling the maximum residue limit in these samples (25 mg kg(-1)). The results showed that the use of nanoparticles in LSL systems may provide novel and simple analytical methods for the screening of contaminants in the agri-food sector.
查看更多>>摘要：This study details the development of on-line two-dimensional liquid chromatography (2D-LC) methods combining cation-exchange chromatography (CEX) and reversed-phase liquid chromatography (RPLC) for the separation of the charge variants of a lysine-linked antibody-drug conjugate (ADC). This combination gives an excellent example of the potential benefits of 2D-LC approaches for the analysis of such complex protein formats. CEX is considered the reference technique for the separation of protein charge variants but its retention mechanism usually requires the use of a high concentration of non-volatile salts, which impedes its compatibility with MS detection. In this context, the use of an on-line 2D-LC-MS approach not only allows on-line desalting and indirect coupling of CEX with mass spectrometry (MS) detection but it also provides increased and complementary information within a single analysis. The first part of this study was devoted to the choice of stationary phases and the optimization of chromatographic conditions in both dimensions. Based on the results obtained in 1D-CEX with ultraviolet detection (UV) and 1D-RPLC with UV and high-resolution mass spectrometry (HRMS) detections, an on-line comprehensive two-dimensional liquid chromatography method combining CEX and RPLC was developed. The last part of this study was devoted to the identification of the separated species using HRMS detection and in the comparison of three ADC samples exposed to different durations of thermal stress.
查看更多>>摘要：Sweat sensor has become one of the most important developing directions of in vitro wearable diagnostic device in recent years. Stable sweat collecting device is the key to realize sweat component analysis. In order to ensure that the collected sweat is not subject to component analysis errors caused by evaporation or environmental pollution, mechanical micro-valves were adopted for microfluidic sweat collection devices to realize sealed storage of sweat. However, this poses a challenge to the stability of machining and reusability of the acquisition device. In this work, the Tesla valve without any mechanical structure were introduced into the design of sweat collection chip. And made full use of its diodicity to improve the collection to a certain extent, prevent backflow at the entrance, and restrain the flow at the exit to contact with the outside world. In addition, through optimizing the shunt angle, branch channel parameters of Tesla valve, boosted its diodicity under low flow rate. Furthermore, a sweat storage chamber with baffle structure that can achieve maximum static storage area was adopted to form a whole sweat collection chip. The design was verified through the flow experiment of methylene blue and methyl red indicators on the chip. Through modification of the filter paper fixed in the collection chamber, the colorimetric analysis of glucose and pH was realized. This device may provide new inspirations for the development of wearable sweat sensor.
查看更多>>摘要：Hydrogen peroxide (H2O2) detection with high sensitivity plays an important role in biomedical research and food engineering. By combining colorimetry and surface enhanced Raman spectroscopy (SERS), we synthetize a novel H2O2 dual-sensor constructed via TMB-Fe3O4@AuNPs. In the presence of H2O2, the peroxide model enzyme might catalyze the oxidation of 3,3',5,5' - tetramethylbenzidine (TMB) as blue charge transfer complex (CTC) for colorimetry, and then facilitate the sensitivity improvement of SERS detection. The achieved results show that in colorimetry, the linear range is from 40 mu M to 5.5 mM with the detection limit of 11.1 mu M; in SERS detection, the linear range is from 2 nM to 1 mu M with the detection limit of 0.275 nM. Clearly, this mutual reference strategy improves both the detection limit of colorimetry and the sensitivity of SERS detection. Moreover, this colorimetry/SERS dual-sensor constructed via TMB-Fe3O4@AuNPs is successfully applied to the H2O2 detection in plasma and milk, indicating the excellent performance and flexibility.
查看更多>>摘要：This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARSCoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)(3)](2+) anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)(3)](2+)/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.
查看更多>>摘要：A simple and low-cost method of fabricating an optical fiber for a surface plasmon resonance (SPR) sensor was proposed. The method is based on the electroless nickel plating and subsequent displacement gold plating of the core of the optical fiber. The thickness of the nickel and gold thin films deposited on the core of the optical fiber could be controlled by measuring the reflected light intensity from the tip of the optical fiber during the plating processes. The sensitivity and resolution of the SPR sensor with the fabricated optical fiber in the refractive index range from 1.333 to 1.348 were 1324.3 nm/RIU and 7.6 x 10(-4) RIU, respectively. The developed SPR sensor was successfully used in the determination of immunoglobulin A (IgA) in human saliva. The IgA quantification results obtained by the SPR sensor were in excellent agreement with those obtained by conventional enzyme-linked immunosorbent assay using a 96-well microtiter plate.
查看更多>>摘要：As one of primary biomarkers of ovarian cancer in early stages, beta-galactosidase (beta-Gal) is significant in the discovery and diagnosis of the disease. In this work, we constructed a multi-signal sensing platform based on silicon nanoparticles (Si NPs) for beta-Gal activity determination. When beta-Gal was introduced to the sensing system, 2-Nitrophenyl beta-D-galactopyranoside (ONPG) could be converted to o-Nitrophenol (o-NP), which had a characteristic absorption peak at 416 nm and the colorless solution turned yellow. The fluorescence emission of Si NPs at 450 nm can be greatly quenched by o-NP as a consequence of inner filter effect (IFE). This dual-signal fluorometric and colorimetric determination approach could be utilized to detect beta-Gal in the range of 2-120 U/L and 6-120 U/L. The limits of detection were 1.36 U/L and 1.07 U/L, respectively. This sensing platform could be successfully utilized to detect beta-Gal in real samples. Additionally, a visual detection method was designed to achieve quantitative analysis of beta-Gal with the assistance of the smartphone.
查看更多>>摘要：Magnetic nanoparticles (NPs) cloaked with cell membranes expressing high levels of the epidermal growth factor receptor (EGFR) have been used to screen for EGFR-targeting active compounds in traditional Chinese medicine (TCM) formulations. However, previous strategies involved physical immobilization of the biomaterials on the surface of the nanocarrier, resulting in highly unstable platforms since the biological materials could dislodge easily. Chemical bonding of biomaterials to the nanoparticles surface can improve the stability of the biomimetic platforms. In this study, membrane fragments from cells expressing SNAP-Tag-EGFR (ST-EGFR) were immobilized on the surface of magnetic NPs. The ST-EGFR magnetic cell membrane nanoparticles (ST-EGFR/MCMNs) showed greater stability, and higher binding capacity, selectivity adsorption of gefitinib after 7 days compared to the un-immobilized magnetic cell membrane nanoparticles (EGFR/MCMNs). The ST-EGFR/MCMNs were used to screen for the EGFR-targeting active compounds of Zanthoxyli Radix (ZR), and identified toddalolactone and nitidine chloride. The latter significantly inhibited the proliferation of EGFR-overexpressing cancer cells, and was more effective compared to gefitinib. This innovative technology can be used to rapidly screen for active compounds from complex extracts, and aid in drug discovery.
查看更多>>摘要：It is of great significance to reveal the molecular distribution images in biological tissues, which has led to the bloom of mass spectrometry imaging. Unfortunately, its application is encountering the resistance of high technical barriers and equipment cost, as well as the inability to image substances that cannot be desorbed or ionized, or cannot be separated by their mass-to-charge ratios. Herein presented is a complementary and costeffective method called capillary array electrophoresis (CAE) imaging. To have the information of molecules and their spatial location, a gridding cutter was fabricated to orderly dissect a tissue section into a leakproof array of micro wells enclosed by the grid-blade arrays. After in situ extraction and fluorophore-labeling of analytes, the samples in the wells were directly subjected to CAE-LIF (laser-induced fluorescence), and the molecular distribution images were depicted with the separated peaks. The practicability was demonstrated by CAE imaging of rat brain tissue sections with amino acid neurotransmitters (e.g., glutamine, 4-aminobutyric acid, alanine, glutamic acid and aspartic acid) as targets. The resultant images showed the global differences of molecular distributions, with a spatial resolution of 1000 mu m that was presently determined by the well width but ultimately by the bore size of capillary (down to 10-50 mu m). CAE imaging can hence be promising for its low cost, low technical barriers and abundant mechanisms to separate the charged and non-charged, chiral and nonchiral substances.
查看更多>>摘要：The use of magnetic nanoparticles shows a steadily increasing technical importance. Particularly in medical technology disciplines such as cancer treatment, the potential of these special particles is increasing rapidly. Magnetic nanoparticles are particles with a submicron size, and consist mostly of magnetite-containing composites. An important quality parameter of such particles is a particle size distribution as narrow as possible, which can only be obtained to a certain degree by synthesis. Apart from ultracentrifugation, there are so far only methods on an analytical scale to narrow the size distribution as a post-processing step. We present a method based on magnetic chromatography, by which high separation efficiencies at yields of up to 99.9% are achieved. The novel technique is based on a competition between the magnetic interaction of the nanoparticles and the separation matrix, as well as the hydrodynamic forces. Furthermore, the method is extended using a continuous mode, namely simulated moving bed chromatography, to obtain potent space-time yields of up to 2.94 g/(L*h). For those reasons, this novel continuous magnetic chromatography method offers high potential for large-scale refinement of magnetic nanoparticles while fulfilling sophisticated quality criteria for high-technology applications.
NSTL
Elsevier