查看更多>>摘要：? 2021Sensitive and accurate early detection of fetal growth restriction (FGR) is of vital importance in the development of the fetus during pregnancy and even the health of future life. Here, an ultrasensitive and straightforward surface-enhanced Raman scattering (SERS)-based double targets detection in pregnancy is implemented by utilizing functionalized Ag nanoparticles (AgNPs). Through fabricating 4-MPA antibody-modified AgNPs on the lateral flow assay (LFA) strips as the SERS nanotags, the target proteins in blood samples from pregnancy were accurately captured, which further quantizing PI3K and CRAF in unprocessed blood. This strategy warrant excellent selectivity and sensitivity with the limits of detection (LODs) are 0.76 fg mL?1 for PI3K and 0.61 fg mL?1 for CRAF, leading reliable quantification for these two targets. Meanwhile, the feasibility of this assembly was testified by comparing with conventional ELISA method, and the results showed that a high degree of consistency was obtained in these two detection assays. This SERS-colorimetric dual-signal LFA strip can provide a novel strategy for early diagnosis of fetal-related disorders, which is essential for disease diagnosis and treatment guidance during pregnancy.
查看更多>>摘要：? 2022 Elsevier B.V.Nanozymes with multiple activities have drawn immense interest owing to their great prospect in biochemical analysis. Fabricating nanomaterials-based artificial enzymes for multiple-enzyme mimetic activity is a significant challenge. This paper reports a sensitive biosensing platform to mimic the peroxidase, oxidase, and catalase-like activity by bimetallic CuPd embedded holey carbon nitride (CuPd@H–C3N4). Owing to the combination of porous H–C3N4 and bimetallic CuPd nanoparticles, the CuPd@H–C3N4 exhibited a large specific surface area, extremely high mobility and catalytic activity of electrons, resulting in remarkable triple-enzyme mimetic activity. Owing to the excellent oxidase/peroxidase-like activities of CuPd@H–C3N4, a visual colorimetric and ultrasensitive fluorometric biosensing platform was established for the discriminatory detection of glutathione (linear range: 2–40 μM) and glucose (linear range: 0.1–40 μM) in physiological fluids, respectively. The fluorescence detection system showed ultrahigh sensitivity toward H2O2, with a linear range of 30–1500 nM. In addition, a one-step glucose detection strategy was proposed to replace the traditional, complicated two-step detection method, which simplifies the operation steps and improves the detection efficiency. The assay presented in this paper offers an effective multiple-enzymes mimicking detection platform that broaden its promising applications in biomedicine analysis and monitoring.
查看更多>>摘要：? 2022Viral diseases are the primary source of death, making a worldwide influence on healthcare, social, and economic development. Thus, diagnosis is the vital approach to the main aim of virus control and elimination. On the other hand, the prompt advancement of nanotechnology in the field of medicine possesses the probability of being beneficial to diagnose infections normally in labs as well as specifically. Nanoparticles are efficiently in use to make novel strategies because of permitting analysis at cellular in addition to the molecular scale. Henceforth, they assist towards pronounced progress concerning molecular analysis at the nanoscale. In recent times, magnetic nanoparticles conjugated through covalent bonds to bioanalytes for instance peptides, antibodies, nucleic acids, plus proteins are established like nanoprobes aimed at molecular recognition. These modified magnetic nanoparticles could offer a simple fast approach for extraction, purification, enrichment/concentration, besides viruses’ recognition precisely also specifically. In consideration of the above, herein insight and outlook into the limitations of conventional methods and numerous roles played by magnetic nanoparticles to extract, purify, concentrate, and additionally in developing a diagnostic regime for viral outbreaks to combat viruses especially the ongoing novel coronavirus (COVID-19).
查看更多>>摘要：? 2022Aristolochic acid I (AAI) as one of the major aristolochic acids (AAs) can cause progressive aristolochic acid nephropathy (AAN), which has been widely investigated since the early 1990s. Besides renal diseases, it has been recently revealed that AAI can induce liver damage. In this study, we report the molecular mapping of liver tissue sections from AAI-exposed mice using atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI MS) and show the distinct metabolic alterations when compared to the control group. We first used renal tissue sections to evaluate the performance of AP-MALDI MSI in spatial discrimination of different morphological regions. Then, the hepatic tissues from the AAI-induced and the control mice were analyzed, displaying rich metabolic profiles from both groups. Orthogonal partial least squares-discriminant analysis (OPLS-DA) is used to show complete separation of the two groups. A machine learning algorithm–least absolute shrinkage and selection operator (lasso) is used for statistical analysis of a total of 11,726 pixels of imaging data extracted from 3 normal liver and 3 AAI-exposed liver tissue sections, generating a classifier with high accuracy (99.81%). In total, 16 m/z values, including small metabolites and lipid species, are selected to discriminate AAI-exposed liver tissues. Finally, we explore the potentially impacted pathways using metabolomics pathway analysis (MetPA), indicating multiple metabolic pathway alterations including taurine and hypotaurine metabolism, glycerophospholipid metabolism, D-Glutamine and D-glutamate metabolism, and arachidonic acid metabolism, which provides new insights in AAI-induced hepatotoxicity.
查看更多>>摘要：? 2022 Elsevier B.V.The work presents innovative quadruple disk iridium, platinum, and iridium-platinum voltammetric electrodes with a special design, dedicated to the testing of samples with a complex organic composition. Noble metal wires are tightened in one silver rod, and therefore each of them acts as a single sensor. It was demonstrated that the signals of the iridium-platinum sensor combine the electrode responses constructed from one metal, which increases the possibilities and range of applications of this sensor, and it can be used as an electronic tongue. These single and combined noble metal electrodes were successfully verified to profile the seasonal variability of honey collected from an apiary in Ma?opolska (voivodeship in Poland). Data obtained by the differential pulse voltammetry, according to the principles of green chemistry, without using any reagents, were interpreted by principal component analysis, preceded by the optimized variable selection procedure. The best results in distinguishing 12 honeys were obtained using a multimetallic electrode. The classification model calculated using deep convolutional neural networks indicated the proper belonging of honeys to the groups with 100% accuracy for the training and validation set. The proposed solution proved that noble metals quadruple disk electrodes are a promising tool supporting voltammetric profiling of samples and this strategy, considering deep learning, can be developed to a large extent.
查看更多>>摘要：? 2022 Elsevier B.V.A novel and disposable biosensor based on superoxide dismutase (SOD) immobilized on gold metallized polycaprolactone electrospun polymeric fibers (PCl/Au) has been developed for the determination of superoxide (O2??) in cell culture media. SOD biosensors were constructed employing three immobilization methods: cross-linking with EDC/NHS at a cysteine self-assembled monolayer (PCl/Au/SODCYS), biopolymer encapsulation with chitosan (PCl/Au/SODCHI) and cross-linking with glutaraldehyde (PCl/Au/SODGA). Scanning electron microscopy was performed at the three different biosensors to evaluate their surface morphologies. Biosensors were employed for the electrochemical detection of superoxide by fixed potential amperometry at different applied potentials, with two distinct enzymatic mechanisms being proposed: i) the reduction of the enzymatically generated peroxide, at ?0.3 V, for which the PCl/Au/SODCHI biosensor presented the highest value of sensitivity of 40.1 μA mM?1 cm?2, and ii) the regeneration of the enzyme catalytic copper centre, at +0.3 V, for which the PCl/Au/SODCYS biosensor had the highest sensitivity value of 16.1 μA mM?1 cm?2. The proposed recognition mechanisms were further confirmed by cyclic voltammetric measurements, which enabled also to determine the amount of immobilized electroactive SOD, with highest value corresponding to the PCl/Au/SODCYS biosensor. The biosensors with best analytical performance, PCl/Au/SODCYS and PCl/Au/SODCHI, were further investigated for stability and selectivity, with best results for the PCl/Au/SODCYS, chosen for superoxide monitoring in cell culture media. The study is promising for future application of PCl/Au/SODCYS for the on-line superoxide monitoring of superoxide in cell cultures, grown directly on the biosensor itself.
查看更多>>摘要：? 2021O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). O-GlcNAc turnover influences the dynamic interactions of CAPs with chromatin and thereby regulates gene expression. Therefore, both global profiling of O-GlcNAc chromatin-associated proteins (OCAPs) and genome-wide mapping of their DNA binding sites are invaluable for understanding the functions of OCAPs and the regulatory machinery of O-GlcNAcylation on gene transcription. However, it is difficult to conduct genome- and proteome-wide OCAP studies using the widely adopted chromatin immunoprecipitation (ChIP) method due to the lack of highly O-GlcNAc-specific panantibodies. Therefore, we developed a chemical enrichment method (AFT-OCAP) for simultaneously profiling OCAPs and mapping their binding DNA via mass spectrometry (MS) analysis and DNA sequencing. In our method, we developed an alkynyl-functionalized trimethylpiperidine (AFT) reagent to perform highly efficient chemical derivatizations of azide-labeled OCAP-DNA complexes. The reversible affinity between the immobilized anti-trimethylpiperidine antibody resin and AFT reagent leads to specific enrichment and efficient elution of the OCAP-DNA complexes for both MS identification and sequencing. Deep coverage of OCAPs was achieved from HeLa cells, including 1951 O-GlcNAc peptides from 1136 O-GlcNAc chromatin-associated transcription factors and cofactors (TFCs) using HCD fragmentation and 669 O-GlcNAc sites using EThcD fragmentation. In addition, the distributions of O-GlcNAcylation across the genome and the dynamic interactions of OCAPs upon O-GlcNAc regulation were obtained.
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