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Brazilian Archives of Biology and Technology
Instituto de tecnologia do Parana
Brazilian Archives of Biology and Technology

Instituto de tecnologia do Parana

1516-8913

Brazilian Archives of Biology and Technology/Journal Brazilian Archives of Biology and TechnologySCIISTP
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    Enteromorpha intestinalis Derived Seaweed Liquid Fertilizers as Prospective Biostimulant for Glycine max

    Mathur, ChetnaRai, SaumyaSase, NikhilKrish, Suthindhiran...
    8页
    查看更多>>摘要：In the present study, the potential of seaweed liquid fertilizer (SLF) of marine algae Enteromorpha intestinalis was evaluated for its effect on seed germination, yield, biochemical parameters and pigment characteristics of Glycine max. E. intestinalis was collected form Mandapam coast of Gulf of Mannar, Tamil Nadu, and the dried seaweeds were used for the preparation of SLF. G. max seeds were germinated with four different concentrations (20, 40, 60, and 100%) of SLF; its growth and yield parameters were evaluated and compared with chemical fertilizer and control. The morphological and bio-chemical parameters such as seed germination (100%), root (6.6cm) and shoot length (5.4 cm), carbohydrates (0.098 mg/g), protein (0.56 mg/g), pigment (0.444 mg/g chl a; 1.073 mg/g chl b; 3.70 mg/g carotenoids) of the plant was found maximum at a concentration of 60% SLF. The phenol content (3.25 mg/g) was maximum in 40% SLF. The GC-MS analysis of SLF revealed the presence of notable benzoic compounds involved in plant growth promotion. Results showed that E. intestinalis derived SLF was potential biostimulant for G. max. Thus, marine algae based fertilizer could be an effective and alternate to the chemical fertilizers emphasizing the need for systematic evaluation programme for SLF on various crops.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    In vitro Antimicrobial Assay of Actinomycetes in Rice Against Xanthomonas oryzae pv. oryzicola and as Potential Plant Growth Promoter

    Hata, Erneeza MohdSijam, KamaruzamanAhmad, Zainal Abidin MiorYusof, Mohd Termizi...
    12页
    查看更多>>摘要：The aim of this work was to in vitro assay the antimicrobial activity of actinomycetes in rice against Xanthomonas oryzae pv. oryzicola and as potential plant growth promoter. A total of 92 actinomycete strains were isolated from different rice plant components and field locations. Of these, only 21.74% showed antagonistic activity against the Xoc pathogen. Molecular identification via 16s rRNA amplification revealed that 60% of the active antagonistic strains belonged to the genus Streptomyces. Isolates that demonstrated the highest antagonistic activity were also able to produce hydrolytic enzymes and plant growth-promoting hormones. Combination of preliminary screening based on in vitro antagonistic, hydrolytic enzyme and plant growth hormone activity facilitated the best selection of actinomycete candidates as evidenced by strains classification using cluster analysis (Ward's Method). Results from the preliminary screening showed that actinomycetes, especially Streptomycetes, could offer a promising source for both biocontrol and plant growth-promotion agents against BLS disease in rice.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    Assessment of the Potential Role of Silymarin Alone or in Combination with Vitamin E and/ or Curcumin on the Carbon Tetrachloride Induced Liver Injury in Rat

    Faddah, LailaSharaf, Iman A.Mohamed, Azza M.Al-Rasheed, Nawal...
    10页
    查看更多>>摘要：The aim of this study was to investigate the effective role of silymarin either alone or in a combination with vitamin E and/or curcumin against the toxic impact of carbon tetrachloride (CCl4) induced liver injury The results revealed that administration of silymarin alone or in a combination with vitamin E and/or curcumin for 21 consecutive days, 24 h after CCl4 injection to rats, markedly ameliorated DNA damaged and apoptosis markers in rat livers, roinflammatory markers including tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein (CRP) in rat livers as well as interleukin 6 (IL-6), interferon gamma (IFN-gamma) and vascular endothelial growth factor (VEGF) in rat sera. These treatments also could ameliorated the alteration in cytochrome P450 2E1 (CYP2E1) activity in livers of CCl4 intoxicated rats as well as the increase in the serum alanine aminotransferase (ALT) compared with CCl4 intoxicated untreated rats. The present biochemical results are supported by histo-pathological examination. In conclusion, silymarin in a combination with vitamin E and curcumin was the most effective treatment in alleviating CCl4 induced liver damage and this may support the use of this combination as an effective treatment against liver damage induced by toxic agents.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation

    Guelfi, MarieliMaioli, Marcos AntonioTavares, Marco AurelioMingatto, Fabio Erminio...
    11页
    查看更多>>摘要：The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in a dose-dependent manner and dissipated the mitochondrial membrane potential that was accompanied by a reduction in ATP concentration and a disruption of intracellular Ca2+ homeostasis. The cell viability was affected by fipronil with higher potency in hepatocytes that were isolated from the normal rats, which indicated that the metabolism of this insecticide increased its toxicological potential. The results of this study indicated that the toxicity of fipronil to the hepatocytes was related to the inhibition of mitochondrial activity, which led to decreased ATP synthesis and a consequent alteration in intracellular Ca2+ homeostasis and ultimately resulted in cell death.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    Genes Regulating Maternal Recognition of Pregnancy in Domestic Animals: an Update

    Mor, AvantikaMondal, SukantaReddy, Ippala JanardanaSoumya, N. P....
    10页
    查看更多>>摘要：Early embryonic mortality is one of the main sources of reproductive wastages and major constraints for full exploitation of the production potential of livestock. The survivality of embryo during early embryonic life is mostly dependent on the efficiency with which the maternal recognition of pregnancy (MRP) is established. Maternal recognition of pregnancy involves molecular dialogue between the trophoblast of conceptus and uterine endometrium. Embryonic development to the blastocyst stage and uterine differentiation to the receptive environment are crucial for successful establishment of the embryo-uterine cross-talk that leads to the initiation and progression of successful implantation. Unravelling the complex intricate molecular and cellular dialogues between the conceptus and uterine environment will facilitate development of strategies to augment early embryo survivality.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    Godoy & Godoy Compression Sleeve in the Treatment of Arm Lymphedema: New Concepts for Materials

    de Godoy, Jose Maria Pereirade Godoy, Ana Carolina PereiraGodoy, Maria de Fatima Guerreiro
    5页
    查看更多>>摘要：The aim of this work was to study on a new low-elastic textile that fulfilled the criteria of fabrics for the manufacture of compression garments to treat lymphedema. The evolution of Godoy & Godoy compression sleeves was performed by following the patients treated for arm lymphedema. Sixty-six patients with ages ranging from 35 to 83 years and a mean of 58.8 years were included in this study. Diagnosis, by the clinical evaluation confirmed by the volumetry, was defined as a volume difference of more than 200 mL between the arms. All the participants were submitted to treatment sessions once, or two times weekly in an outpatient setting. The material used for the compression sleeve in this study was commercialized in Brazil under the name of Gorgurao (R). When high alterations in the pattern of sleeves were made, the patients were monitored by weekly volumetric assessments. The criterion to maintain the modifications in the design was that the hand did not present with edema. By the end of the study, the design of the sleeve was changed so as not to use compression therapy of the hands in 81.8% of the cases; 12.2% continued with compression of the hand, 3.0% stopped using compression completely and 3.0% used only a glove. Thus, Godoy & Godoy compression sleeves could be an efficient option for compression in the treatment of arm lymphedema as they provided better independence in day-to-day activities.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    In vitro Anticancer Property of Yellow Pigment from Streptomyces griseoaurantiacus JUACT 01

    Prashanthi, KuruvalliVaralakshmi, Kilinger NadumaneSuryan, Sandeep
    8页
    查看更多>>摘要：Despite the complications in isolation of pigments, microbial pigments are increasingly gaining the attention of researchers because of their broad range therapeutic potentials, especially against cancer. In this study the cytotoxic and anti proliferative potentials of yellow pigment from Streptomyces griseoaurantiacus JUACT 01 isolated from soil are investigated. The effect of pigment treatment on the growth and proliferation of in vitro cervical cancer cells (HeLa) and liver cancer cells (Hep G2) was tested by various methods. Significant cytotoxicity was observed with IC (50) values as low as 1.5 and 1.8 mu g /mL with HeLa and Hep G2 cells respectively. The pigment exhibited non toxic effects on human lymphocytes. Decrease in the number of viable cells, presence of apoptotic bodies, nuclear condensation and sheared DNA were distinctly observed in pigment treated cancer cells. The biochemical test and the infrared (IR) spectra indicated the probable carotenoid presence in the TLC purified pigment fraction. High Performance Liquid Chromatography (HPLC) analysis of the TLC purified yellow pigment showed a single large peak with a retention time of 9.90 min and m/z value corresponding to the peak was found to be 413.22 showing 100% relative abundance.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    Morphological Plasticity and Gas Exchange of Ligustrum lucidum WT Aiton in Distinct Light Conditions

    Larcher, LeticiaNogueira, GuilhermeBoeger, Maria Regina
    9页
    查看更多>>摘要：The objective of this study was to understand the different morpho-physiological strategies of Ligustrum lucidum, an invasive species occurring in Brazilian forest fragments under heterogeneous light conditions. Ten individuals of L. lucidum were selected and evaluated for morphological of the leaves and physiological traits. For morphological parameters were evaluated: length, width, area, angle, petiole length, dried mass, total thickness, palisade parenchyma and spongy parenchyma thickness, abaxial and adaxial epidermis thickness, stomata density, leaf density and specific leaf area. The physiological traits were vapor-pressure deficit, assimilation rate, CO2 substomata concentration, intrinsic water-use efficiency, transpiration rate and stomatal conductance. All the physiological variables and most morphological variables presented significant differences between light conditions. Phenotypic plasticity indexes were not high as expected. However, phenotypic integration among the morphological and physiological attributes appeared to explain better these results, as observed on the relationship among assimilation rates, palisade parenchyma thickness and SLA. Phenotypic integration could increase the species adaptive responses efficiency, making it more competitive to occupy and to establish in new niches.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    Skin Doses on the Lens for Temporomandibular Joint Exam in Cone Beam Computed Tomography

    Linhares de Oliveira, Marcus ViniciusAlmeida Andrade, Marcos ElyBatista, Wilson OttoFlores Campos, Paulo Sergio...
    5页
    查看更多>>摘要：The aim of this study was to evaluate the kerma at the surface of the lens in TMJ CBCT and to derive the equivalent dose. An anthropomorphic phantom of the head and neck manufactured by Radiation Support Devices (model RS-230) was used. The dosimetric measurements were obtained by using fourteen thermoluminescent dosimetry (TLD) dosimeters (LiF: Mg, Ti), divided in two pairs (one pair for each eye) and positioned on the surface of the phantom, per scanner evaluated. The tomographic images were acquired in three types of CBCT equipment (CS 9000, Gendex GXCB 500 and i-CAT). Values of equivalent dose obtained were: 5.82 mSv (CS 9000); 5.38 mSv (Gendex GXCB 500) and 7.98 mSv (i-CAT), which varied in accordance with the scanner and the exposure factors used in the image acquisition. The Gendex GXCB 500 used larger FOV and higher kV, resulting in levels close to those obtained on the CS 9000, while larger doses were associated with the i-CAT. The dose values associated with TMJ radiological procedures should be performed with awareness and appropriateness due to sensitivity of the lens.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana

    Sperm Quality and Testicular Histomorphometry of Wistar Rats Supplemented with Extract and Fractions of Fruit of Tribulus terrestris L.

    Pinho Muniz Oliveira, Nelma NeylanneRodrigues Felix, Matheus AugustoSilva Pereira, Tauany CostaPessoa Rocha, Luiz Gustavo...
    7页
    查看更多>>摘要：The aim of this study was to assess the sperm quality and testicular histomorphometry of Wistar rats supplemented with extract and fractions of fruits of Tribulus terrestris L. The ethanolic extract was obtained by dynamic maceration of spray-dried fruit. This extract was fractionated by liquid-liquid partition, using increasing polarity solvents. Twenty male rats were separated in four groups, with five rats in each group. The control was supplemented with distilled water, while the others were daily given the ethanolic extract, hexanic or aqueous fraction soluble in methanol in a dose of 42 mg.kg(-1).day(-1) for 70 days. Sperm was obtained from the right epididymal tail for the analysis of motility, count, morphology and viability. The testicular weight of groups supplemented with ethanolic extract and aqueous fraction soluble in methanol was higher when compared to the control. The gonadosomatic index increased in the group supplemented with ethanolic extract. The nuclear, cytoplasmic and individual volume of Leydig cells increased in supplementation with hexanic and aqueous fractions soluble in methanol. It was concluded that the extract influenced the spermatogenesis, while hexanic and aqueous fractions soluble in methanol promoted the changes in the intertubular compartment. Therefore, Tribulus terrestris did not improve the sperm quality of the rats.
      原文链接:
    • NSTL
    • Inst Tecnologia Parana