De Araujo, Valter LoureiroDos Santos, Marcio FerreiraSchnadelbach, Alessandra SelbachDe Castro Nunes, Jose Marcos...
13页
查看更多>>摘要：Two Leptolyngbya-like cyanobacterial strains were isolated from two marine benthic environments on the Brazilian coast. These strains were cultured and characterized based on their morphology, molecular, and ultrastructural data. The two taxa were identified mainly by 16S rRNA gene phylogeny and 16S-23S ITS secondary structures since their morphology is similar to members of Leptolyngbya senso lato. The phylogenetic analysis indicated that our strains belong to Euryhalinema genus (Leptolyngbyaceae), with one strain close to type species E. mangrovii AP9F (MK402979), and the other described as a new species, E. epiphyticum sp. nov. Morphologically, both strains form green mats, had trichomes without sheath, and their thylakoid disposition is the same as described for Leptolyngbyaceae. The secondary structures Box B and D1-D1' of the internal transcribed spacer (16S-23S ITS) also corroborated our proposal of the new species E. epiphyticum. These findings constitute the first description of a new-to-science species for this genus outside Indian marine environments. Also, it expands the knowledge on Euryhalinema systematic.
查看更多>>摘要：On the basis of expansive molecular phylogenetic analyses of the genus Miliusa (Annonaceae) utilising up to seven plastid DNA regions, four major clades are identified: clades A, B, C and D. Members of clade C possess axillary inflorescences and crescent-shaped to semicircular glandular structures at the base or (slightly) higher inside the inner petals. In this clade, accessions of M. mollis are recovered as a monophyletic group, divided into two major clades. One of them is recognised as a new species, M. microphylla, which differs from the other (M. mollis) in the following characters: pedicel length, as well as glandular structures and indumentum on the inner petals. The new species is endemic to southeastern Thailand, whereas M. mollis has a much wider distribution (central, eastern, northeastern, northern and peninsular Thailand plus Cambodia and Vietnam). In addition, M. glandulifera, a new record for Thailand, is retrieved as the sister group of a clade composed of M. microphylla and M. mollis. The name M. glandulifera is lectotypified and the description ofM. glandulifera is emended, with the information on mature monocarps added. The conservation status of M. microphylla and M. glandulifera is provisionally assessed. A revised key to the species in clade C in Thailand is provided. The phylogenetic position of M. nakhonsiana and M. sessilis in clade C, as well as of M. chantaburiana and M. eupoda in clade B is confirmed for the first time.
Antonio Vazquez-Garcia, J.Santiago Rosales-Martinez, C.Acevedo-Rosas, Raul
13页
查看更多>>摘要：Graptopetalum trujilloi (subg. Glassia, Crassulaceae), a new haplostemonous species from San Gabriel municipality, Jalisco, Mexico is described and illustrated. Its morphologically closest species is G. rosanevadoense. It differs from the latter in having a smaller habit, more numerous primary branches per inflorescence, larger mean flower density per 10 cm of the scape, fewer flowers per basal branch, smaller flower diameter, petals greenish with transversal inconspicuous red stripes, narrower petals, filaments greenish to distally reddish and carpels abaxially obtuse. A key to the haplostemonous species of Graptopetalum is provided and, according to IUCN criteria, the species was assessed as critically endangered (CR).
Kim, Do-HyunLee, Nam-JuYoon, Sung-RoKim, Taehee...
13页
查看更多>>摘要：Porphyrosiphon annulatus sp. nov. belonging to Microcoleaceae, Oscillatoriales was collected from the moist soil under the rock in the Gwanggyo Mountain of Suwon City, Gyeonggi Province, Korea and cultured. Using the raw and cultured samples of the collected strains, we observed their morphological features through LM and TEM. Furthermore, their 16S rRNA gene and 16S-23S rRNA intergenic transcribed spacer (ITS) sequences were determined to be used phylogenetic analyses. We affirmed that thick sheath of the cells possessed transversely annular and longitudinal lamellations. The ultrastructure of thylakoids was a radial arrangement, which corresponds with a characteristic of the Microcoleaceae. In addition, P. annulatus displays the most similar morphology with type species P. notarisii; however, it differently holds the transversely annular lamellations with a shorter cell length and relatively dense trichome. Our 16S rRNA gene phylogeny showed that P. annulatus formed a clade with P. notarisii, and their DNA similarity was calculated to be 96.2%. The family Microcoleaceae was found to be paraphyletic with a distinct linage of the genus Porphyrosiphon. The ITS sequence of P. annulatus was unique in length and nucleotide composition, with different secondary structures of D1-D1MODIFIER LETTER PRIME and Box-B helices compared to those of close relatives. Overall, morphology and genetic traits of the Korean Porphyrosiphon suggest that P. annulatus should be considered to be a novel species.
NSTL
Magnolia Press