Grunert, Ryan K. A.Kunkel, Melanie R.Radisic, RebeccaNemeth, Nicole M....
4页
查看更多>>摘要：Mange is a contagious skin disease caused by different mite species affecting numerous domestic and wild animals, worldwide. This report details notoedric mange in an eastern cottontail (Sylvilagus floridanus) and in a marsh rabbit (Sylvilagus palustris) from Florida, USA. Clinical examination revealed similar gross lesions including poor nutritional condition, multifocal alopecia and hyperkeratosis. Skin scrapings from both rabbits revealed numerous subcutaneous mites identified as Notoedres centrifera, a species previously only associated with rodents, primarily squirrels. Mites from both rabbits were identified based on morphology and confirmed by sequencing the internal transcribed spacer-2 (ITS-2) region. These cases emphasize the need for continued surveillance and accurate diagnostic evaluation to determine the cause and characterization of the skin disease, while distinguishing it from other potential pathogens that may manifest similarly in rabbits, such as Notoedres cati, Sarcoptes scabiei or Psoroptes cuniculi.
Rauff-Adedotun, A. A.Syafyqah, S. NuurSoleha, R. NurShahida, S. Noranis...
5页
查看更多>>摘要：Blastocystis is an enigmatic intestinal protist of humans and many animals. There is growing interest in its potential as an enteric pathogen, and the possible role of domestic and in-contact animals as reservoirs for human infection. The purpose of this study was to determine the prevalence of Blastocystis infection in fishes, poultry, and caprine in Penang, Malaysia. A total of 353 faecal samples/intestinal contents, consisting of 123 intestinal contents from freshwater fishes, 96 intestinal contents of commercially barn-reared chickens, 84 intestinal contents of barn-reared quails, and 50 faecal samples of caprine (29 from meat goats and 21 from dairy goats) were collected. Faecal sample/intestinal content from each animal was subjected to in-vitro cultivation method using Jones' medium supplemented with 10% horse serum. The respective colonization frequencies for dairy and meat goats were 47.6% and 31.0%, whereas 26.1% was recorded for chickens and 16.7% for quails. None of the freshwater fishes were found infected with Blastocystis. The organism was most commonly seen as spherically shaped vacuolated forms and cell diameter was significantly larger in poultry than in caprine. For further studies, molecular characterization of Blastocystis in poultry and livestock animals in the study area is highly recommended.
Romero-Salas, DoraSanchez-Montes, SokaniBravo-Ramos, Jos L.Sanchez-Otero, Maria G....
4页
查看更多>>摘要：Zoological gardens represent specialised centres for the preservation of biological inventories and genetic diversity, allowing the recognition of multiple species in critical conservation categories. However, the close coexistence of multiple species of vertebrates that may be associated with various species of ectoparasites may be the cause of the transmission of multiple infectious agents, among which tick-borne pathogens stand out. In these areas, several animal species usually live in a small space and proximity to other wildlife, visitors and keepers. In Mexico, little is known about the disease agents transmitted by arthropods in zoological gardens. For this reason, the aim of this study was to identify the presence of Babesia/Theileria in animals maintained in captivity. As a part of a project identifying vector-borne pathogens in wildlife, 24 animals were sampled in the Miguel Angel de Quevedo zoo. Molecular identification of Babesia/Theileria was realised through amplification of a fragment of the mitochondrial cytB gene and the ribosomal 18S-rDNA. Two neotropical camelids (Lama glama) tested positive (2/3 = 66.6%) to Babesia bigemina. Our results represent the first record of B. bigemina in animals in captivity in a zoological garden in Mexico and the first finding of this haemoparasite in neotropical camelids in Mexico.
查看更多>>摘要：Human and dog health are inextricably linked, and although our relationship with dogs brings numerous benefits for our well-being, it is known that they can transmit disease. Bariloche is a Patagonian tourist city with het-erogeneous social composition. This cross-sectional study evaluates the population of free-roaming dogs and their intestinal parasites in relation to the socioeconomic level of the city's human population. Census areas were used as survey units, stratified in three levels according to socioeconomic status. The free-roaming dog popu-lation was estimated by walking around each census area. Eight fresh feces per census count area were collected and analyzed using coproparasitological flotation tests, and CoproELISA was used to detect Echinococcus sp. A total of 858 free-roaming dogs were registered along 40.9 km, with significant differences between socioeco-nomic strata: the highest numbers were found in the lowest income strata. Of the feces collected, 39.2% tested positive for parasites, those associated with a lower socioeconomic level having higher percentages of positive feces and a greater number of species. Eight species of helminths were found, some of which were zoonotic, such as Echinococcus sp., Toxocara canis, and Dibothriocephalus latus. The presence of parasites can be explained by the number of free-roaming dogs per census count area. The free-roaming dogs generally have owners, and their parasitic infection is strongly associated with the socioeconomic level of the population. The main problem is irresponsible pet care, which generates healthy conditions for both dogs and humans. Thus, both dogs and humans deserve effective ethical public policies.
Waine, KatieBartley, Paul M.Cox, AlistairNewsome, Reuben...
9页
查看更多>>摘要：Eosinophilic myositis in bovine striated muscle thought to be caused by a hypersensitivity reaction to the degradation of Sarcocystis tissue cysts, is a rare reason for carcase condemnation in the United Kingdom. This paper describes the identification of Sarcocystis cruzi associated with lesions of generalised eosinophilic myositis in three English beef carcases, by gross and histopathological examination followed by PCR with subsequent sequencing. Samples from two unaffected animals were also examined. Although sarcocystosis caused by S.cruzi is not considered a public health risk, the clinically affected carcases were deemed unfit for human consumption due to the extensive lesions affecting meat quality. We believe this to be the first report from the UK describing the molecular-based identification of Sarcocystis cruzi in meat affected and unaffected with eosinophilic myositis.
查看更多>>摘要：Tick-borne pathogens (TBPs) pose an increased health and productivity risk to livestock in sub-Saharan Africa. Information regarding TBPs infecting small ruminants in Kano metropolis is scarce. Therefore, we investigated the molecular epidemiology of tick-borne pathogens of economic importance from sheep and goats in Kano, Nigeria using Polymerase chain reaction (PCR). A total of 346 blood DNA samples were collected from small ruminants and analyzed for TBPs using PCR and sequencing. Risk of infection was determined for age, sex, breed and animal species. Our results indicate the absence of piroplasmids (Babesia/Theileria) and Rickettsia spp. infections. The overall prevalence for Anaplasma spp. was 9.25% (32/346) with a higher prevalence in goats 13.59% (25/184) compared with sheep 4.32% (7/162). With respect to age of animals, goats >4 years had the highest prevalence of 32.45% (11/37) which differs significantly (P = 0.0059) compared with other age categories. Cross breed goats had a prevalence of 15.63% (5/32) compared with Kano brown breed 14.08 (20/142). Sex significant difference (P = 0.029) was observed in the goats with females having the highest prevalence 20.89% (14/67) compared with males 9.40% (11/117). Furthermore, with regards to sheep, no significant difference (P > 0.05) was observed with respect to age and breed. Finally, no significant difference (P > 0.05) was observed with the prevalence of Anaplasma spp. due to Body condition score (BCS) in both sheep and goats. Conclusively, the occurrence of TBPs in small ruminants is low. Continuous efforts in tick control must be sustained to ensure high productive yield and reduced disease burden associated with TBPs of sheep and goats in Kano metropolis.
查看更多>>摘要：Ascaris roundworms are of public health and socio-economic importance worldwide. They are conventionally attributed to two taxa - A. lumbricoides infecting principally human and A. suum infecting principally pig. Phylogenomic analysis has revealed that Ascaris worms from both human and pig are represented in Clades A and B. A recent study indicates that the Ascaris worms from human and pig in Thailand belong to Clade A. We examined adult Ascaris worms from human and pig in Thailand by means of the partial sequences of three mitochondrial genes (cox1, cox2 and nad1) and concatenation of these genes. Phylogenomic analysis indicates that two isolates (H1,H2) of A. lumbricoides from human belonged to Clade B; one isolate (H3) belonged to Clade A (based on cox1, cox2 and concatenated sequences) or as an outlier to Clades A and B (based on nad1 sequences). All the eight isolates of A. suum from pig clustered in Clade A. The partial nad1 and the concatenated sequences revealed two lineages of A. suum isolates which were distinct from the two A. lumbricoides isolates of Clade B. It is evident that greater genetic diversity, and a more robust phylogeny, could be uncovered by the application of multiple genes. In sum, the present study reveals the presence in Thailand of A. lumbricoides from human in Clades A and B which necessitates appropriate treatment and control measures; Clades A and B have been reported to contain haplotypes of Ascaris worms from both human and pig in other parts of the world. A country wide study is needed to elucidate the identity, distribution, prevalence, cross transmission, genetic diversity and phylogeny of the Ascaris worms in Thailand.
Gakuya, F.Maina, L. G. M.Maingi, N.Ng'ang'a, C. J....
6页
查看更多>>摘要：The diversity, prevalence and intensity of parasitic infections are influenced by factors such as movement patterns and land area available to the host. Zebras are known to harbour various genera of gastrointestinal helminths and facilitate parasite movement across large geographic areas through migration. Despite the harmful effects of helminths and their consequences on host population dynamics, little is known regarding helminth infection patterns in migratory, resident, and sedentary zebras. This study compared the diversity, prevalence, and intensity of helminth infections in zebras with different host movement patterns and space availability. Faecal samples were collected from migratory, resident, and sedentary groups of zebras between 2014 and 2015 and screened for helminth infection. They were infected by diverse groups of helminths including nematodes, trematodes and cestodes with the most prevalent being strongyle type nematodes. No significant difference in the diversity and prevalence was observed between the "three groups". Larvae were also recovered and identified species, cyathostomes, Strongylus edentatus, S.vulgaris, S. equinus, and Trichostronglus in order of abundance. The intensity of infection with the strongyle type nematodes, was highest in the migratory, then sedentary and lowest in resident zebras. It is plausible that the migratory zebras have higher energy demands and poor nutrition, resulting in reduced immune function hence high nematode burdens. Similarly, the sedentary population had high nematode burdens possibly due to high pasture contamination and depressed immunity due to the artificially restricted movement. The latter observation is particularly important in the design and size of sanctuaries in relation to parasitism.
NSTL
Elsevier