查看更多>>摘要：Affinity chromatography is a cornerstone of bispecific antibody (bsAb) purification, with resin selection playing a critical role in developing downstream processes to ensure both process efficiency and product quality. Unlike monoclonal antibody purification, where Protein A chromatography is the gold standard for antibody capture, affinity chromatography in bsAb purification is often employed not only for capture but also for the removal of hard-to-eliminate product-related impurities. This study demonstrates that affinity resin selection can be effectively guided by analysing the domain composition of the target bsAb molecule and its potential impurities. Using faricimab, a CH1-CL CrossMab, as a model, Protein L - an affinity resin targeting the variable region of the light chain - was predicted to be the most effective affinity chromatography due to its different binding avidity towards faricimab from its major product-related impurities. Validation through screening four different types of affinity chromatography, each binding to distinct regions of faricimab, confirmed this prediction. Under optimized elution conditions, the purification process achieved ~73% purity from -30% in the culture with ~86 % monomeric yield, as well as decent removal of host cell proteins and host cell DNA.
查看更多>>摘要：Affinity chromatography is a cornerstone of bispecific antibody (bsAb) purification, with resin selection playing a critical role in developing downstream processes to ensure both process efficiency and product quality. Unlike monoclonal antibody purification, where Protein A chromatography is the gold standard for antibody capture, affinity chromatography in bsAb purification is often employed not only for capture but also for the removal of hard-to-eliminate product-related impurities. This study demonstrates that affinity resin selection can be effectively guided by analysing the domain composition of the target bsAb molecule and its potential impurities. Using faricimab, a CH1-CL CrossMab, as a model, Protein L - an affinity resin targeting the variable region of the light chain - was predicted to be the most effective affinity chromatography due to its different binding avidity towards faricimab from its major product-related impurities. Validation through screening four different types of affinity chromatography, each binding to distinct regions of faricimab, confirmed this prediction. Under optimized elution conditions, the purification process achieved ~73% purity from -30% in the culture with ~86 % monomeric yield, as well as decent removal of host cell proteins and host cell DNA.
查看更多>>摘要：Deoxynivalenol (DON) and zearalenone (ZEN) are toxic Fusarium mycotoxins that frequently contaminate food and feed, posing significant public health risks. Among mitigation strategies, biological methods using lactic acid bacteria (LAB; e.g., Lactobacillus spp.) show particular promise, as these microorganisms can effectively biode-grade mycotoxins into less toxic or inactive metabolites. This study explored the binding and biotransformation capabilities of Lactobacillus brevis components (S-layer, aryl-alcohol dehydrogenase, carboxylesterase, and lip-oteichoic acid repeats) using computational docking and Fourier transform infrared (FTIR) spectroscopy. Docking simulations revealed stronger binding affinities for ZEN than DON, involving interactions with hydroxyl groups, oxygen atoms, alkyl chains, and aromatic rings. Enzymes formed stable complexes with mycotoxins, suggesting biotransformation potential. FTIR spectra after 24 hours of incubation revealed mycotoxin adsorption through interactions with bacterial cell wall components, indicated by C-H and C-C peak shifts. For DON, changes in OH and C=O peaks suggested oxidation-reduction, likely mediated by aryl-alcohol dehydrogenase, producing 3-keto-DON and 3-epi-DON. For ZEN, shifts in OH, C-O, and C=O peaks indicated lactone ring hydrolysis, likely catalyzed by carboxylesterase. These findings highlight L. brevis as a promising biocontrol agent that detoxifies DON and ZEN through binding and biotransformation, offering a potential strategy to mitigate mycotoxin contamination in food and feed.
查看更多>>摘要：Deoxynivalenol (DON) and zearalenone (ZEN) are toxic Fusarium mycotoxins that frequently contaminate food and feed, posing significant public health risks. Among mitigation strategies, biological methods using lactic acid bacteria (LAB; e.g., Lactobacillus spp.) show particular promise, as these microorganisms can effectively biode-grade mycotoxins into less toxic or inactive metabolites. This study explored the binding and biotransformation capabilities of Lactobacillus brevis components (S-layer, aryl-alcohol dehydrogenase, carboxylesterase, and lip-oteichoic acid repeats) using computational docking and Fourier transform infrared (FTIR) spectroscopy. Docking simulations revealed stronger binding affinities for ZEN than DON, involving interactions with hydroxyl groups, oxygen atoms, alkyl chains, and aromatic rings. Enzymes formed stable complexes with mycotoxins, suggesting biotransformation potential. FTIR spectra after 24 hours of incubation revealed mycotoxin adsorption through interactions with bacterial cell wall components, indicated by C-H and C-C peak shifts. For DON, changes in OH and C=O peaks suggested oxidation-reduction, likely mediated by aryl-alcohol dehydrogenase, producing 3-keto-DON and 3-epi-DON. For ZEN, shifts in OH, C-O, and C=O peaks indicated lactone ring hydrolysis, likely catalyzed by carboxylesterase. These findings highlight L. brevis as a promising biocontrol agent that detoxifies DON and ZEN through binding and biotransformation, offering a potential strategy to mitigate mycotoxin contamination in food and feed.
查看更多>>摘要：Peanut shell powder was washed with water and then calcined to produce biochar, which acted as an adsorbent and biofilm supporter for the removal of atrazine (ATR). Biochar sample prepared by washing 6 h (named BC-W) achieved the highest biodegradation amount (Q_m, 12.48 mg/g). The adsorption process of ATR on BC-W involved hydrogen bonding and π-π interactions. We found that the removal rate of ATR by biodegradation was 6.34-6.75 times that of adsorption, with an ATR concentration of 30 mg/L under half aeration. The results of high-throughput sequencing showed that there were two dominant bacterial phyla in the BC-W samples after biofilm culture: Firmitcutes (51.89 %) and Proteobacteria (47.79 %), among which Bacillus, Clostridium (sensu stricto), and Pseudomonas, played a major role in the biodegradation of ATR. These findings showed that biochar could be employed as a low-cost adsorbent for the removal of pesticides from wastewater, also a biofilm supporter for enhanced biodegradation of organic pollutants.
查看更多>>摘要：Peanut shell powder was washed with water and then calcined to produce biochar, which acted as an adsorbent and biofilm supporter for the removal of atrazine (ATR). Biochar sample prepared by washing 6 h (named BC-W) achieved the highest biodegradation amount (Q_m, 12.48 mg/g). The adsorption process of ATR on BC-W involved hydrogen bonding and π-π interactions. We found that the removal rate of ATR by biodegradation was 6.34-6.75 times that of adsorption, with an ATR concentration of 30 mg/L under half aeration. The results of high-throughput sequencing showed that there were two dominant bacterial phyla in the BC-W samples after biofilm culture: Firmitcutes (51.89 %) and Proteobacteria (47.79 %), among which Bacillus, Clostridium (sensu stricto), and Pseudomonas, played a major role in the biodegradation of ATR. These findings showed that biochar could be employed as a low-cost adsorbent for the removal of pesticides from wastewater, also a biofilm supporter for enhanced biodegradation of organic pollutants.
Olga BabichSvetlana IvanovaAlina BakhtiyarovaOlga Kalashnikova...
35-51页
查看更多>>摘要：Plant-based cosmetics are made with a variety of cosmetic ingredients as the base, but they do not contain harmful synthetic chemical compounds that are toxic to humans. Plant-based cosmetics are safer and more natural than traditional cosmetics. The market for natural beauty products is constantly expanding, in parallel with consumer demand. These cosmetics have therapeutic properties ranging from anti-aging to hair care products. Plant-based cosmetics appeal to consumers due to their greater affordability and fewer side effects. This paper aims to analyze the plants that appeal to the cosmetic industry, as well as their primary applications. Currently, there is a lack of research reports on the antioxidant, anti-inflammatory, and antimicrobial potential of plants. In this study, plant bioactive compounds that showed significant bactericidal activity against antibiotic-resistant Gram-positive bacteria in vitro were investigated. Plant extracts act as adjuvants to antibiotics, enhancing the activity of several commonly used antibiotics against both Gram-positive and Gram-negative antibiotic-resistant bacteria without cytotoxicity to cells. In addition, biologically active compounds from plants show significant effects on wound treatment and healing in vivo. It is known that medicinal plants with biological antioxidant, anti-inflammatory, and antimicrobial components can be used in the treatment of skin infections and wound healing.
Olga BabichSvetlana IvanovaAlina BakhtiyarovaOlga Kalashnikova...
35-51页
查看更多>>摘要：Plant-based cosmetics are made with a variety of cosmetic ingredients as the base, but they do not contain harmful synthetic chemical compounds that are toxic to humans. Plant-based cosmetics are safer and more natural than traditional cosmetics. The market for natural beauty products is constantly expanding, in parallel with consumer demand. These cosmetics have therapeutic properties ranging from anti-aging to hair care products. Plant-based cosmetics appeal to consumers due to their greater affordability and fewer side effects. This paper aims to analyze the plants that appeal to the cosmetic industry, as well as their primary applications. Currently, there is a lack of research reports on the antioxidant, anti-inflammatory, and antimicrobial potential of plants. In this study, plant bioactive compounds that showed significant bactericidal activity against antibiotic-resistant Gram-positive bacteria in vitro were investigated. Plant extracts act as adjuvants to antibiotics, enhancing the activity of several commonly used antibiotics against both Gram-positive and Gram-negative antibiotic-resistant bacteria without cytotoxicity to cells. In addition, biologically active compounds from plants show significant effects on wound treatment and healing in vivo. It is known that medicinal plants with biological antioxidant, anti-inflammatory, and antimicrobial components can be used in the treatment of skin infections and wound healing.
David Spressao de Lima JuniorIlana SessakDaniela RemonattoJulian Paul Martinez Galan...
52-62页
查看更多>>摘要：This study presents a novel 3D-printed poly(lactic acid) (PLA) carrier for the covalent immobilization of a commercial glucoamylase. Enzyme carriers were functionalized by aminolysis with ethylenediamine (EDA) and activated with glutaraldehyde. Since covalent immobilization using glutaraldehyde as activating agent involves the formation of imine bonds which are inherently unstable, the use of borohydride as a reducing agent to stabilize these imine bonds after the functionalization step was considered. The highest enzymatic activity (13.68 U g~(-1) carrier) was obtained when immobilization was performed at pH 10 using NaBH_4 (immobilization yield of 32.80 % ± 0.51 %). Optimal activity conditions were pH 4.92 and 50 ℃ for the soluble enzyme and pH 5.5 and 50 ℃ for the immobilized enzyme. The operational stability of immobilized glucoamylase was evaluated for 10 consecutive reaction cycles (of 10 minutes each) and the enzyme maintained 65.19 % of its original activity at the end of the third cycle. Starch saccharification in a stirred tank reactor by immobilized glucoamylase reached 95 % conversion after 12 h and 74 % conversion after 12 h in the first reuse cycle. These findings demonstrate the potential of PLA for glucoamylase immobilization, offering promising prospects for efficient and sustainable starch hydrolysis in industrial enzymatic processes.
David Spressao de Lima JuniorIlana SessakDaniela RemonattoJulian Paul Martinez Galan...
52-62页
查看更多>>摘要：This study presents a novel 3D-printed poly(lactic acid) (PLA) carrier for the covalent immobilization of a commercial glucoamylase. Enzyme carriers were functionalized by aminolysis with ethylenediamine (EDA) and activated with glutaraldehyde. Since covalent immobilization using glutaraldehyde as activating agent involves the formation of imine bonds which are inherently unstable, the use of borohydride as a reducing agent to stabilize these imine bonds after the functionalization step was considered. The highest enzymatic activity (13.68 U g~(-1) carrier) was obtained when immobilization was performed at pH 10 using NaBH_4 (immobilization yield of 32.80 % ± 0.51 %). Optimal activity conditions were pH 4.92 and 50 ℃ for the soluble enzyme and pH 5.5 and 50 ℃ for the immobilized enzyme. The operational stability of immobilized glucoamylase was evaluated for 10 consecutive reaction cycles (of 10 minutes each) and the enzyme maintained 65.19 % of its original activity at the end of the third cycle. Starch saccharification in a stirred tank reactor by immobilized glucoamylase reached 95 % conversion after 12 h and 74 % conversion after 12 h in the first reuse cycle. These findings demonstrate the potential of PLA for glucoamylase immobilization, offering promising prospects for efficient and sustainable starch hydrolysis in industrial enzymatic processes.
NETL
NSTL
Elsevier