期刊,Cytology and genetics 2025年59卷3期_国家学术搜索

Changes in Interleukin-17A Content in the Blood of Patients with Diabetes after COVID-19

V. M. PushkarevL. K. SokolovaN. I. LevchukO. I. Kovzun...

241-247页

查看更多>>摘要：The level of interleukin-17A (IL-17A) was studied in the blood of 82 patients with diabetes mellitus (DM) who had contracted coronavirus disease 2019 (COVID-19) 2-4 years ago. IL-17A was determined using enzyme immunoassay kits. It was shown that the concentration of the cytokine was significantly higher in diabetic patients and in people who had COVID-19. A difference in cytokine levels in the blood of patients who had mild and severe forms of COVID-19, as well as a positive effect of vaccination, was noted. IL-17A concentration increased with diabetes duration of more than 15 years, with body mass index more than 30kg/m~2 and glycated hemoglobin (HblAc) > 7.5%. COVID-19 additionally increased these indicators. There is a positive effect of metformin on IL- 17A concentration in patients with diabetes and high interleukin response to COVID-19 in the absence of biguanide treatment. A positive effect of insulin and type 2 sodium-dependent glucose cotransporter inhibitors (iSGLT-2) was also noted. The amount of IL-17A increases in blood plasma with pathological changes in the left ventricular ejection fraction, glomerular filtration rate, and albuminuria. The study of the concentration of IL-17A in blood plasma depending on the age of patients showed a tendency for decrease in the amount of interleukin with age. A high level of IL-17A is observed in the blood plasma of patients with diabetes who had COVID-19 2-4 years ago comparable to an acute disease. In the absence of treatment of patients with diabetes with glucose-lowering drugs in COVID-19, the level of IL-17A reaches high values. The study of the concentration of IL-17A in blood plasma depending on the age of patients showed a tendency to a decrease in the amount of interleukin in older age groups.

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Changes in Interleukin-17A Content in the Blood of Patients with Diabetes after COVID-19

V. M. PushkarevL. K. SokolovaN. I. LevchukO. I. Kovzun...

241-247页

查看更多>>摘要：The level of interleukin-17A (IL-17A) was studied in the blood of 82 patients with diabetes mellitus (DM) who had contracted coronavirus disease 2019 (COVID-19) 2-4 years ago. IL-17A was determined using enzyme immunoassay kits. It was shown that the concentration of the cytokine was significantly higher in diabetic patients and in people who had COVID-19. A difference in cytokine levels in the blood of patients who had mild and severe forms of COVID-19, as well as a positive effect of vaccination, was noted. IL-17A concentration increased with diabetes duration of more than 15 years, with body mass index more than 30kg/m~2 and glycated hemoglobin (HblAc) > 7.5%. COVID-19 additionally increased these indicators. There is a positive effect of metformin on IL- 17A concentration in patients with diabetes and high interleukin response to COVID-19 in the absence of biguanide treatment. A positive effect of insulin and type 2 sodium-dependent glucose cotransporter inhibitors (iSGLT-2) was also noted. The amount of IL-17A increases in blood plasma with pathological changes in the left ventricular ejection fraction, glomerular filtration rate, and albuminuria. The study of the concentration of IL-17A in blood plasma depending on the age of patients showed a tendency for decrease in the amount of interleukin with age. A high level of IL-17A is observed in the blood plasma of patients with diabetes who had COVID-19 2-4 years ago comparable to an acute disease. In the absence of treatment of patients with diabetes with glucose-lowering drugs in COVID-19, the level of IL-17A reaches high values. The study of the concentration of IL-17A in blood plasma depending on the age of patients showed a tendency to a decrease in the amount of interleukin in older age groups.

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Activation of Autophagy During Microsporogenesis and Tapetogenesis in Angiosperms

O. A. KravetsS. G. PlokhovskaT. V. ChugunkovaA. I. Yemets...

248-258页

查看更多>>摘要：In this research the involvement of autophagy in the development of microsporogenesis in shepherd's purse (Capsella bursa-pastoris) as a representative of dicotyledons and in Siebold's plantain lily (Hosta sieboldiana) and Virginia spiderwort (Tradescantia virginiana) as representatives of monocotyledons was investigated. It was shown that microsporogenesis in the studied species is accompanied by the activation of autophagy, which is associated with both the onset and completion of meiosis and corresponds to the accumulation and degradation of regulators involved in meiotic division and tapetogenesis. The presence of cyto-mixis in meiosis prophase may serve as additional confirmation of the regulatory role of autophagy in meiosis. It was confirmed that autophagy is involved in the functioning and degradation of the tapetum. The activation of autophagy may accompany the formation of tetrads of microspores (C. bursa-pastoris), the functioning and degradation of the tapetum (H. sieboldiana), as well as the formation of tetrads and the final degradation of the tapetum (T. virginiana). This may be because the studied species differ in the type of tapetum (secretory and plasmodial). This difference between tapetum types is not clearly diagnosed, and varieties are differentiated in terms of reorganization, intensity of autophagy, and time of degradation of the tapetum tissue within the secretory type. The obtained results allow for the conclusion that, in general, the functioning and degradation of the tapetum in the studied monocots are accompanied by more intense autophagy than in the representative of dicot.

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Activation of Autophagy During Microsporogenesis and Tapetogenesis in Angiosperms

O. A. KravetsS. G. PlokhovskaT. V. ChugunkovaA. I. Yemets...

248-258页

查看更多>>摘要：In this research the involvement of autophagy in the development of microsporogenesis in shepherd's purse (Capsella bursa-pastoris) as a representative of dicotyledons and in Siebold's plantain lily (Hosta sieboldiana) and Virginia spiderwort (Tradescantia virginiana) as representatives of monocotyledons was investigated. It was shown that microsporogenesis in the studied species is accompanied by the activation of autophagy, which is associated with both the onset and completion of meiosis and corresponds to the accumulation and degradation of regulators involved in meiotic division and tapetogenesis. The presence of cyto-mixis in meiosis prophase may serve as additional confirmation of the regulatory role of autophagy in meiosis. It was confirmed that autophagy is involved in the functioning and degradation of the tapetum. The activation of autophagy may accompany the formation of tetrads of microspores (C. bursa-pastoris), the functioning and degradation of the tapetum (H. sieboldiana), as well as the formation of tetrads and the final degradation of the tapetum (T. virginiana). This may be because the studied species differ in the type of tapetum (secretory and plasmodial). This difference between tapetum types is not clearly diagnosed, and varieties are differentiated in terms of reorganization, intensity of autophagy, and time of degradation of the tapetum tissue within the secretory type. The obtained results allow for the conclusion that, in general, the functioning and degradation of the tapetum in the studied monocots are accompanied by more intense autophagy than in the representative of dicot.

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Genetic Polymorphism of Invasive Species of Knotweed (Reynoutria) Assessed by the matK and rpl32-trnL (UAG) Regions of Chloroplast DNA

Y. O. TynkevichA. S. CherkazianovaI.I. ChorneyI.I. Panchuk...

259-269页

查看更多>>摘要：An important model system for studying the role of genetic diversity and hybridization in plant invasions is the species complex of the genus Reynoutria Houtt. Within the secondary distribution range, two species of this genus are widespread, R.japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai, as well as their derivatives, the hexaploid R. × bohemica Chrtek & Chrtkovd and the tetraploid R. x moravica (Hodalovd and Mereda) Olshanskyi and Antonenko, which are recognized as separate species. The genetic diversity of the species of the genus Reynoutria in Ukraine is still almost unexplored by molecular methods. In this work, we determined chloroplast haplotypes for samples of R. japonica, R. sachalinensis and R. × bohemica from Ukraine and other European countries and compared them with haplotypes of Reynoutria from the primary distribution range in China and Korea. The genetic diversity of R.japonica from the primary distribution range was significantly higher compared to European samples, which are mainly represented by the haplotype J 1.1. At the same time, we identified haplotypes J1.2 and J1.3 specific to the Eastern European area, which probably arose as a consequence of the divergence of the chloroplast genome within the secondary distribution range. Of the five samples morphologically identified as R. × bohemica, three carry the haplotype Jl.l, which is consistent with the idea that R.japonica var. japonica was involved as a maternal form in the formation of R. × bohemica. However, a chloroplast haplotype identical to R. sachalinensis was detected in two samples from the Alpine region of Europe. These samples likely represent another hybrid species of R. × moravica. Therefore, the use of chloroplast DNA markers is crucial for identifying the donor of maternal subge-nomes in hybrid forms of the genus Reynoutria.

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Genetic Polymorphism of Invasive Species of Knotweed (Reynoutria) Assessed by the matK and rpl32-trnL (UAG) Regions of Chloroplast DNA

Y. O. TynkevichA. S. CherkazianovaI.I. ChorneyI.I. Panchuk...

259-269页

查看更多>>摘要：An important model system for studying the role of genetic diversity and hybridization in plant invasions is the species complex of the genus Reynoutria Houtt. Within the secondary distribution range, two species of this genus are widespread, R.japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai, as well as their derivatives, the hexaploid R. × bohemica Chrtek & Chrtkovd and the tetraploid R. x moravica (Hodalovd and Mereda) Olshanskyi and Antonenko, which are recognized as separate species. The genetic diversity of the species of the genus Reynoutria in Ukraine is still almost unexplored by molecular methods. In this work, we determined chloroplast haplotypes for samples of R. japonica, R. sachalinensis and R. × bohemica from Ukraine and other European countries and compared them with haplotypes of Reynoutria from the primary distribution range in China and Korea. The genetic diversity of R.japonica from the primary distribution range was significantly higher compared to European samples, which are mainly represented by the haplotype J 1.1. At the same time, we identified haplotypes J1.2 and J1.3 specific to the Eastern European area, which probably arose as a consequence of the divergence of the chloroplast genome within the secondary distribution range. Of the five samples morphologically identified as R. × bohemica, three carry the haplotype Jl.l, which is consistent with the idea that R.japonica var. japonica was involved as a maternal form in the formation of R. × bohemica. However, a chloroplast haplotype identical to R. sachalinensis was detected in two samples from the Alpine region of Europe. These samples likely represent another hybrid species of R. × moravica. Therefore, the use of chloroplast DNA markers is crucial for identifying the donor of maternal subge-nomes in hybrid forms of the genus Reynoutria.

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Modulating the Biological Effect of Berberine via Its Immobilization on Different Polymer Nanocarriers

N. SkorokhydR. PanchukO. ZaichenkoN. Mitina...

270-280页

查看更多>>摘要：Berberine is an isoquinoline alkaloid obtained from different medicinal herbs, including Berberis spp., Coptis spp., and Hydrastis spp. (Imanshahidi and Hosseinzadeh, 2008). It is widely used in medicine and the pharmaceutical industry to prevent and treat diseases. However, despite the prominent pharmacological properties of the berberine, there are some limitations to its therapeutic application. Polymer nanoparticles may be an effective platform for overcoming these limitations. This study is the first to obtain stable aqueous complexes of berberine using three types of polymer carriers. Their synthesis involved polymer-analogue transformations and copolymerising PEG-methacrylate (PEGMA) (Riabtseva et al., 2016). This approach helped control the structural and molecular mass characteristics of novel nanocomposites of berberine, their ability to form micellae and their colloid-chemical properties, affecting the biocompatibility of the obtained composites. In this work, a comparative in vitro study of the cytotoxic activity of berberine complexes on 3 different branched polymeric carriers was performed: (1) poly(VEP-co-GMA)-graft-mPEG, (2) poly(VEP-co-GMA)-graft-pEtOX, and (3) poly(PEGMA-co-DMM). The investigation of cell viability in vitro demonstrated that the used berberine nanocomposites on the polymer carriers had higher toxicity to the tumor cells than free berberine. The degree of a decrease in cell viability under the effect of berberine nanocomplexes (PC-PEG-Berb, PC-pEtOx-Berb, PC-PEGMA-Berb) depends on the type of the polymer carrier. At the same time, native polymer carriers (PC-PEG, PC-pEtOx, PC-PEGMA) in a free form do not induce a considerable decrease in cell viability in the concentrations required for the delivery of 50 μM berberine, which demonstrates their biocompatibility. The obtained results indicate the high potential of using berberine complexes with polymeric nanocarriers in cancer chemotherapy.

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Modulating the Biological Effect of Berberine via Its Immobilization on Different Polymer Nanocarriers

N. SkorokhydR. PanchukO. ZaichenkoN. Mitina...

270-280页

查看更多>>摘要：Berberine is an isoquinoline alkaloid obtained from different medicinal herbs, including Berberis spp., Coptis spp., and Hydrastis spp. (Imanshahidi and Hosseinzadeh, 2008). It is widely used in medicine and the pharmaceutical industry to prevent and treat diseases. However, despite the prominent pharmacological properties of the berberine, there are some limitations to its therapeutic application. Polymer nanoparticles may be an effective platform for overcoming these limitations. This study is the first to obtain stable aqueous complexes of berberine using three types of polymer carriers. Their synthesis involved polymer-analogue transformations and copolymerising PEG-methacrylate (PEGMA) (Riabtseva et al., 2016). This approach helped control the structural and molecular mass characteristics of novel nanocomposites of berberine, their ability to form micellae and their colloid-chemical properties, affecting the biocompatibility of the obtained composites. In this work, a comparative in vitro study of the cytotoxic activity of berberine complexes on 3 different branched polymeric carriers was performed: (1) poly(VEP-co-GMA)-graft-mPEG, (2) poly(VEP-co-GMA)-graft-pEtOX, and (3) poly(PEGMA-co-DMM). The investigation of cell viability in vitro demonstrated that the used berberine nanocomposites on the polymer carriers had higher toxicity to the tumor cells than free berberine. The degree of a decrease in cell viability under the effect of berberine nanocomplexes (PC-PEG-Berb, PC-pEtOx-Berb, PC-PEGMA-Berb) depends on the type of the polymer carrier. At the same time, native polymer carriers (PC-PEG, PC-pEtOx, PC-PEGMA) in a free form do not induce a considerable decrease in cell viability in the concentrations required for the delivery of 50 μM berberine, which demonstrates their biocompatibility. The obtained results indicate the high potential of using berberine complexes with polymeric nanocarriers in cancer chemotherapy.

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COMP, TLR2, TLR4, and NFKB1 Gene Expression in Synovial Fluid Cells of Patients with Osteoarthritis after SARS-CoV-2 Infection

A. S. HuetK. O. DvorshchenkoD. M. GrebinykS. V. Borodin...

281-288页

查看更多>>摘要：Coronavirus disease 2019, induced by SARS-CoV-2 virus (severe acute respiratory syndrome-related coronavirus 2), has led to a huge negative impact on people's health all around the globe, including in Ukraine. The potential effects of coronavirus infection on the course of osteoarthritis, one of the most widespread chronic degenerative joint illnesses, remains unclear. The aim of this work was to analyze the expression of COMP, TLR2, TLR4, and NFKB1 genes in synovial liquid cells as well as to establish the concentration of cytokines (IL-6, IL-8), TLR-2, and COMP in blood plasma of osteoarthritic patients that have had the SARS-CoV2 infection. The research included 75 men, aged from 45 to 55 years. The volunteers were divided into the following groups: the first group (n= 25) was conditionally healthy people, the second group (n = 25) was Ⅱ-Ⅲ degree knee joint osteoarthritis patients, and the third group consisted of 25 patients with Ⅱ-Ⅲ degree knee joint osteoarthritis after having had COVID-19. The expression levels of COMP, TLR2, TLR4, and NFKB1 genes in knee joint synovial liquid cells was measured by RT-qPCR. The concentration of IL-6, IL-8, TLR-2, and COMP was estimated with enzyme-linked immunosorbent assay. More significant decrease in the COMP gene expression in osteoarthritic patients that had COVID-19 was shown compared to the group with knee joint osteoarthritis alone on the background of more intensive COMP concentration increase in patients with osteoarthritis after SARS-CoV-2 infection. At the same time, the increase in expression levels of TLR2, TLR4, and NFKB1 was also detected as more evident in osteoarthritic patients after having COVID-19 disease if compared to the group of patients with knee joint osteoarthritis on the background of more substantial increase in IL-6, IL-8, and TLR-2 in osteoarthritic patients after having SARS-CoV-2 infection. Exacerbation of systemic inflammation due to the body's response to viral invasion can cause such a pathological connection. The results indicate the intensification of destructive processes in the cells of the synovial fluid of patients with osteoarthritis after SARS-CoV-2 infection, which may indicate the risk of a more severe course of this disease.

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COMP, TLR2, TLR4, and NFKB1 Gene Expression in Synovial Fluid Cells of Patients with Osteoarthritis after SARS-CoV-2 Infection

A. S. HuetK. O. DvorshchenkoD. M. GrebinykS. V. Borodin...

281-288页

查看更多>>摘要：Coronavirus disease 2019, induced by SARS-CoV-2 virus (severe acute respiratory syndrome-related coronavirus 2), has led to a huge negative impact on people's health all around the globe, including in Ukraine. The potential effects of coronavirus infection on the course of osteoarthritis, one of the most widespread chronic degenerative joint illnesses, remains unclear. The aim of this work was to analyze the expression of COMP, TLR2, TLR4, and NFKB1 genes in synovial liquid cells as well as to establish the concentration of cytokines (IL-6, IL-8), TLR-2, and COMP in blood plasma of osteoarthritic patients that have had the SARS-CoV2 infection. The research included 75 men, aged from 45 to 55 years. The volunteers were divided into the following groups: the first group (n= 25) was conditionally healthy people, the second group (n = 25) was Ⅱ-Ⅲ degree knee joint osteoarthritis patients, and the third group consisted of 25 patients with Ⅱ-Ⅲ degree knee joint osteoarthritis after having had COVID-19. The expression levels of COMP, TLR2, TLR4, and NFKB1 genes in knee joint synovial liquid cells was measured by RT-qPCR. The concentration of IL-6, IL-8, TLR-2, and COMP was estimated with enzyme-linked immunosorbent assay. More significant decrease in the COMP gene expression in osteoarthritic patients that had COVID-19 was shown compared to the group with knee joint osteoarthritis alone on the background of more intensive COMP concentration increase in patients with osteoarthritis after SARS-CoV-2 infection. At the same time, the increase in expression levels of TLR2, TLR4, and NFKB1 was also detected as more evident in osteoarthritic patients after having COVID-19 disease if compared to the group of patients with knee joint osteoarthritis on the background of more substantial increase in IL-6, IL-8, and TLR-2 in osteoarthritic patients after having SARS-CoV-2 infection. Exacerbation of systemic inflammation due to the body's response to viral invasion can cause such a pathological connection. The results indicate the intensification of destructive processes in the cells of the synovial fluid of patients with osteoarthritis after SARS-CoV-2 infection, which may indicate the risk of a more severe course of this disease.

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