查看更多>>摘要：In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture (25 μL total volume) that contained 2.0 μL 10 x buffer, 0.45 mmol·L~(-1) dNTPs, 2.0 mmol· L~(-1) Mg~(2+), 2 U Taq DNA polymerase, 0.30 umol· L~(-1) primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style.
查看更多>>摘要：Based on chicken' consensus map issued in 2000, 17 microsatellites near 4 candidate genes such as IGF2, OBR, GDF8 and APOA1 in 4 chromosomes (chromosome 5, 7, 8 and 24) were chosen for polymorphism analysis and construction of linkage map. Combining the technique of PCR and the fluorescent semi-automated detection, genome scanning was performed for 440 chickens, which was derived from China Agricultural University chicken resource families within three generations. The individuals of this resource families were genotyped. The results showed that the number of alleles ranged from 4 to 14; heterozygosity (H) of markers was between 0.3116 and 0.9148. Polymorphic information content (PIC) varied from 0.2672 to 0.8679. Microsatellites along with above-mentioned 4 candidate genes doing as general markers were used to construct linkage map. The spans of 4 linkage maps constructed in the part region of chromosome 5, 7, 8 and 24 were 263.5, 79.9, 206.2 and 104.2 cM, respectively. The order of markers was consistent with that of counterpart of reported consensus map. However, The spans of linkage map were larger than that of consensus map. The constructed linkage maps laid the foundation for mapping quantitative trait loci (QTL) responsible for economically important traits in chicken.
查看更多>>摘要：Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate. In this study the eggs of Yellow catfish (Pelteobagrus fulvidraco) were irradiated by UV suspending in the synthetic ovarian fluid (OF), and the total dosage of UV irradiation was 220 mJ·cm~(-2). Diploid could be induced by heat shock (40℃, 2 min, 3 min) with different time period after fertilization (15-37 min). The result showed that heat shock with 2 min was better than 3 min; there were two apices of induction in 17-21 min and 27-31 min after fertilization. The highest hatching rate was 3.30% at 29 min after fertilization, and the difference between two apices of induction was unobvious.
查看更多>>摘要：A 2 x2 x2 factorial design was adopted to study the effects of temperature, moisture and choline chloride on vitamin A stability in premix. The results indicated that temperature, moisture and choline chloride damaged vitamin A significantly. The regression equations of vitamin A disappearance rate and storage time were as follows: in room temperature (18±3)℃, y=14.368Ln(x)+ 4.1425, R~2=0.978; in high temperature (4℃), y = 22.24Ln(x)+13.27, R~2=0.9918; in low moisture (2%-3%), y = 10.408Ln(x)+9.5418, R~2=0.9322; in high moisture (8%-9%), y = 26.199Ln(x)+7.8741, R~2=0.9949; in the condition of choline chloride free, y = 9.5125Ln(x)+ 8.9869, R~2=0.9826; supplemented with choline chloride, y =27.094Ln (x)+8.4276, R~2 = 0.9984. Temperature had highly significant interaction with moisure and choline chloride on destruction of vitamin A, respectively from the periods of two months storage. However, from the period of the first month storage, the interaction of moisture and choline chloride, as well as the interaction of temperature, moisture and choline destroyed vitamin A remarkably.
查看更多>>摘要：In order to study the mechanism of immunopotentiator, the quantity of T lymphocyte was observed. Total 240 1-day chikens were divided into 3 groups randomly: one contrast group and two groups being drunk immunopotentiator according to the concentration 10 mL·L~(-1) and 5 mL·L~(-1) lasting for 48 d. The number of T lymphocyte in blood was measured by E-rosette when the chikens were at 12-, 24-, 36-, and 48-day. The results showed that the percent of T lymphocyte in the trial group was obviously higher than that of the contrast, and the 10 mL·L~(-1) group was higher than that of the 5 mL·L~(-1) group; the quantity distribution of T lymphocyte in intestinal mucosa lymphoid tissue of 14-day, 21-day chiken was surveyed hi the contrast group and the 10 mL·L~(-1) group with the method of routine histology-slices and ANAE, the results showed that the quantity of T lymphocyte in the 10 mL·L~(-1) group was significantly higher than that of the contrast, which indicated that immunopotentiator increased markedly the quantity of T lymphocyte, and the 10 mL·L~(-1) group was higher than that of the 5 mL ·L~(-1) group.
查看更多>>摘要：It is shown by the result of the dual-cultured experiment that the inhibitory rate of DZW-47 was 60.42%, and the inhibitory rates of R.solani by actinomyces ZLR-2 and ZLR-11 were 43.75% and 43.05%, lower than that of DZW-47. The inhibitory mycelia growth mechanism of different strains on R.solani was quite different, with DZW-3 mainly on the aspect of hyperparasitism, DZW-21 on the synergism of hyperparasitism and metabolite, DZW-47 on the synergism of nutrient competition and secondary metabolite, ZLR-2 and ZLR-11 on producing secondary metabolite. Controlling efficiency of seedling bed accorded basically with that of the broth. The controlling efficiency of DZW-47, ZLR-2, ZLR-11, DZW-21 and DZW-3 were 97.20%, 95.7%, 94.6%, 93.6% and 89.20%, respectively.
查看更多>>摘要：In sewage sludge composting, adding ash from stove (FA20) inactivated active Cu effectively and the results were significantly better than control. For Zn, Mn, Pb and Cd, satisfying results was achieved by adding ash from stove and phosphorite powder (FA20, PR10), phosphorite powder and lime (PR15, CL10), ash from stove and phosphorite powder (FA10, PR10), and ash from stove and peat (FA20, PT10). Based on their effects of inactivating available forms of heavy metals, ash from stove, phosphorite powder and peat were supposed to be effective inactivators with optimum percentages of 20%, 15% and 10% (FA20, PR15, PT10).
查看更多>>摘要：This paper made a research on a new kind of rice fat mimics, which was obtained from rice powder hydrolyzed by a -amylase. Through the comparison between the yoghurt added with diverse proportions of above mentioned rice fat mimics and the reduced-fat yoghurt without any fat mimics as well as full-fat ones, the effect of the rice fat mimics in different proportions was examined upon the composition, the microstructure, the texture and the sensory evaluation of reduced-fat yoghurt. The results showed that the yoghurts added with rice fat mimics exhibited similar organoleptic attribute, textual characteristics and acceptability compared to those of full-fat controls (P > 0.05), but with lower fat content and looser microstructures.
查看更多>>摘要：Gelatin extracted from deer bone was hydrolyzed for 3.5-120 min. The degree of hydrolysis was higher from Alcalase- hydrolyzed gelatin than that from neutral proteinase-hydrolyzed gelatin. Alcalase-hydrolyzed gelatin exhibited a stronger antioxidant activity than that of neutral proteinase-hydrolyzed gelatin. Hydrolysates showed strong radical-scavenging ability and Fe~(2+)-chelating activity, both of which were influenced by hydrolysis time. Although nonhydrolyzed gelatin displayed a certain antioxidative effect, it was far less than that of hydrolysates. The hydrolysates of deer bone gelatin can work as a radical stabilizer and metal ion chelator to inhibit lipid oxidation.
查看更多>>摘要：Controlled release NPK compound fertilizers were prepared by means of in situ polymerization of monomers on the surface of fertilizer granules at room temperature. Methacrylate, α-methyl acrylic acid, and ethylene dimethylacrylate were used as monomers, Dibenzoyl peroxide as initiator, and cobalt naphthenate, and triethyl amine as promoters. The structures of coating materials were characterized by IR spectra. The thermogravimetric analysis result indicated that the coating materials were of good thermal stability. The mean thickness of single coating measured with screw gauge was ca. 140 μm. The morphologies of uncoated and coated fertilizer granules analyzed by using scanning electron microscopy were changed from porosities and gullies to hills and plain. The release rate of coated compound fertilizers in water could be controlled by the hydrophicity and thickness of coating. The increase in coating hydrophicity caused the increase in release rate of fertilizer. The increase in thickness of coating slowed the release rate.
NETL
NSTL